Identification of A Novel Capsular Polysaccharide Cluster in <i>Lactiplantibacillus plantarum</i> YZH81

ZHAO Fang; HUANG Lu; HUAN Hailin; HUANG Shuting; ZHANG Wenjun; LIANG Qingqing; YANG Yao

doi:10.13386/j.issn1002-0306.2023020075

Volume 44 Issue 23

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(23): 118-126. > DOI: 10.13386/j.issn1002-0306.2023020075

ZHAO Fang, HUANG Lu, HUAN Hailin, et al. Identification of A Novel Capsular Polysaccharide Cluster in Lactiplantibacillus plantarum YZH81[J]. Science and Technology of Food Industry, 2023, 44(23): 118−126. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020075.

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Identification of A Novel Capsular Polysaccharide Cluster in Lactiplantibacillus plantarum YZH81

1.
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China
2.
School of Tourism & Culinary Arts, Zhejiang Business College, Hangzhou 310053, China
3.
Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nangjing 210014, China
4.
Xuzhou Xiangran Agriculture and Animal Husbandry Development Co., Ltd., Xuzhou 221622, China

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Received Date: February 08, 2023
Available Online: October 07, 2023

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Abstract

Abstract

In order to investigate the mechanism of extracellular polysaccharide (EPS) synthesis in Lactobacillus plantarum, a strain of Lactobacillus plantarum YZH81 with high EPS production was selected for the study of extracellular polysaccharide synthesis gene cluster (cps). After whole-genome sequencing, alignment and analysis, it was determined that the genome of YZH81 contained two cps gene clusters, one of which (cps1) had yet to be identified in terms of structure and function, while the other (cps2) was highly homologous to other reported Lactobacillus plantarum strains. The conclusions in this study, the cps1 gene cluster resulted 52.28% reduction in EPS production, accelerated self-aggregation, reduced adhesion and 32.42% reduction in the ability to generated 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH·) in the YZH81∆cps1 strain compared to the wild-type YZH81 strain. The results suggested that the cps1 gene cluster of strain YZH81 was associated with EPS synthesis and established favorable conditions for further studies on the mechanism of EPS biosynthesis in this strain.
- Lactiplantibacillus plantarum,
- exopolysaccharides (EPS),
- capsular polysaccharide cluster (cps cluster),
- adhesion,
- probiotics

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References (33)

References

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