Response Surface Optimization of the QuEChERS Combined with UHPLC-MS/MS Method for Determination of Tetracyclines in Muscle of <i>Channa argus</i>

LI Lichun; LIU Shugui; LIN Jiawei; YIN Yi; ZHENG Guangming; ZHAO Cheng; MA Lisha; SHAN Qi; DAI Xiaoxin; WEI Linting; ZHOU Hao

doi:10.13386/j.issn1002-0306.2022120266

Volume 44 Issue 21

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(21): 216-226. > DOI: 10.13386/j.issn1002-0306.2022120266

LI Lichun, LIU Shugui, LIN Jiawei, et al. Response Surface Optimization of the QuEChERS Combined with UHPLC-MS/MS Method for Determination of Tetracyclines in Muscle of Channa argus[J]. Science and Technology of Food Industry, 2023, 44(21): 216−226. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120266.

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Response Surface Optimization of the QuEChERS Combined with UHPLC-MS/MS Method for Determination of Tetracyclines in Muscle of Channa argus

LI Lichun^{1, 2, 3,},
LIU Shugui^{1, 2, 3},
LIN Jiawei^{1, 2, 3},
YIN Yi^{1, 2, 3, ,},
ZHENG Guangming^{1, 2, 3},
ZHAO Cheng^{1, 2, 3},
MA Lisha^{1, 2, 3},
SHAN Qi^{1, 2, 3},
DAI Xiaoxin^{1, 2, 3},
WEI Linting^{1, 2, 3},
ZHOU Hao^{1, 2, 3}

1.
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
2.
Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species, Ministry of Agriculture and Rural Affairs, Guangzhou 510380, China
3.
Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Guangzhou 510380, China

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Received Date: January 03, 2023
Available Online: October 24, 2023

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Abstract

Abstract

A quick, easy, cheap, effective, rugged and safe pre-treatment method (QuEChERS) was established to evaluate the risk of tetracycline residues in the muscle of Channa argus. The Box-Behnken design (BBD) response surface optimization method was used to optimize the extraction and purification conditions. The samples were extracted with 10 mL acetonitrile (containing 1% formic acid) and 2 mL 0.1 mol/L Na₂EDTA-Mcllvaine buffer solution, and the extracts were purified by 150 mg primary secondary amine（PSA）and 200 mg C₁₈, and detected with Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The result showed that the calibration curve was linear in the range of 0.50~100 ng/mL, with high correlation coefficients (r≥0.999). The limits of detection (LOD) and quantification (LOQ) were 0.25～0.75 μg/kg and 0.84～2.00 μg/kg, respectively. The average recoveries of tetracycline, oxytetracycline, chlortetracycline and doxycycline at three different spiked levels in the muscle of Channa argus were 89.5%～118%, with relative standard deviations (RSDs) of 1.11%～8.88%. This method is simple, sensitive and accurate, and can be used for the rapid quantitative determination of tetracyclines in Channa argus.
- QuEChERS,
- UPLC-MS/MS,
- Channa argus,
- tetracycline,
- risk assessment

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References (43)

References

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