LI Qiuyue, YUAN Mingyang, WU Lina, et al. Protective Effect of Sauchinone on Apoptosis and Oxidative Stress of Islet β Cells in STZ Induced Diabetic Mice[J]. Science and Technology of Food Industry, 2023, 44(16): 420−426. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120010.
Citation: LI Qiuyue, YUAN Mingyang, WU Lina, et al. Protective Effect of Sauchinone on Apoptosis and Oxidative Stress of Islet β Cells in STZ Induced Diabetic Mice[J]. Science and Technology of Food Industry, 2023, 44(16): 420−426. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120010.

Protective Effect of Sauchinone on Apoptosis and Oxidative Stress of Islet β Cells in STZ Induced Diabetic Mice

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  • Received Date: December 14, 2022
  • Available Online: June 18, 2023
  • Objective: To investigate the protective effect of sauchinone (Sch) on apoptosis and oxidative stress of pancreatic β-cells in Streptozocin (STZ)-induced diabetic mice and its relevant mechanisms. Methods: Among 50 male BALB/C mice, 8 mice were randomly selected and grouped to be the normal control group, and the other mice were intraperitoneally received 40 mg/kg STZ. After the confirmation of the onset of diabetes mellitus in 32 mice by measuring the fasting blood glucose level (FBGL), the mice were evenly divided into the model group, low-dose Sch group, high-dose Sch group and metformin-treated positive control group. And the mice in low-dose Sch group and high-dose Sch group were respectively gavaged with 10 or 30 mg/kg Sch, and the mice in metformin-treated positive control group were gavaged with 100 mg/kg metformin once a day for 4 weeks, while the mice in the normal control group and the model group were gavaged with the same volume of saline. During the treatment period, the body weight of each mouse was weekly checked, and at 12 h after the last dosing, the FBGL, the serum insulin concentration, the homeostasis model assessment insulin resistance (HOMA-IR), the serum TNF-α and IL-6 levels were determined. The malondialdehyde (MDA) concentration, the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in pancreatic tissues were measured. Moreover, the pancreatic protein expressions of Bcl-2, Caspase-3 and NF-κB were detected by Western blot. Results: Compared with the model group, the body weights in the high-dose Sch groups checked on the 3rd and 4th week were significantly increased (P<0.05). The FBGL and the serum insulin concentration in Sch-treated groups rose significantly in a dose-dependent manner (P<0.05). The HOMA-IR was significantly decreased (P<0.05). The levels of TNF-α and IL-6 in serum were significantly decreased (P<0.05). The MDA concentrations in the Sch-treated pancreatic tissues decreased, while the SOD and GSH-Px activities increased significantly in a dose-dependent manner (P<0.05). Additionally, the Sch treatments significantly raised the pancreatic Bcl-2 protein expression while lowed the pancreatic Caspase-3 and NF-κB protein expressions in a dose-dependent manner compared with the model group (P<0.05). Conclusions: Sch protects diabetic mice islet β cells, and the mechanism may be mediated by its anti-inflammation and anti-oxidative stress as well as anti-apoptosis actions.
  • [1]
    LI Y, TENG D, SHI D, et al. Prevalence of diabetes recorded in mainland China using 2018 diagnostic criteria from the American diabetes association: National cross sectional study[J]. BMJ,2020,369:m997.
    [2]
    SEMWAL D K, KUMAR A, ASWAL S, et al. Protective and therapeutic effects of natural products against diabetes mellitus via regener-ating pancreatic β-cells and restoring their dysfunction[J]. Phytother Res,2020,35(3):1218−1229.
    [3]
    尤丽, 党娅, 杨彬彦. 蓝莓花青素对2型糖尿病小鼠糖脂代谢的调节作用[J]. 食品工业科技,2022,43(9):381−388. [YOU L, DANG Y, YANG B Y. Regulation effects of blueberry anthocyanins on glucose-lipid metabolism in type 2 diabetic mice[J]. Science and Technology of Food Industry,2022,43(9):381−388.

    YOU L, DANG Y, YANG B Y. Regulation effects of blueberry anthocyanins on glucose-lipid metabolism in type 2 diabetic mice[J]. Science and Technology of Food Industry, 2022, 43(9): 381-388.
    [4]
    DAI B, WU Q X, ZENG C X, et al. The effect of Liuwei Dihuang decoction on PI3K/Akt signaling pathway in liver of type 2 diabetes mellitus (T2DM) rats with insulin resistance[J]. J Ethnopharmacol,2016,19(2):382−389.
    [5]
    GHADGE A A, KUBALEKAR A A. Controversy of oral hypoglycemic agents in type 2 diabetes mellitus: Novel move towards combinationtherapies[J]. Diabetes & Metabolic Syndrome Clinical Research & Reviews,2017,11(Suppl 1):S5−S13.
    [6]
    HANCHANG W, KHAMCHAN A, WONGMANEE N, et al. Hesperidin ameliorates pancreatic β-cell dysfunction and apoptosis in streptozotocin-induced diabetic rat model[J]. Life Sci,2019,235:116858. doi: 10.1016/j.lfs.2019.116858
    [7]
    GURZOV E N, KE P C, AHIGREN U, et al. Novel strategies to protect and visualize pancreatic β-cells in diabetes[J]. Trends Endocrinol Metab,2020,31(12):905−917. doi: 10.1016/j.tem.2020.10.002
    [8]
    WU X, HUANG L T, ZHOU X L, et al. Curcumin protects cardiomyopathy damage through inhibiting the production of reactive oxygen species in type 2 diabetic mice[J]. Biochem Biophys Res Commun,2020,5301(1):15−21.
    [9]
    赵培媛, 唐泳楠, 林上伟, 等. 三白草本草考证[J]. 亚太传统医药,2022,18(7):175−183. [ZHAO P Y, TANG Y N, LIN S W, et al. Herbal textual research of Saururus chinensis (Lour.) Baill[J]. Asia-Pacific Traditional Medicine,2022,18(7):175−183.

    ZHAO P Y, TANG Y N, LIN S W, et al. Herbal textual research of Saururus chinensis(Lour. ) Baill[J]. Asia-pacific Traditional Medicine, 2022, 18(7): 175-183.
    [10]
    何传波, 邓婷, 魏好程, 等. 仙草多糖对细胞氧化损伤的保护作用[J]. 食品科学,2020,41(13):160−168. [HE C B, DENG T, WEI H C, et al. Protective effect of polysaccharide from mesona blumes on oxidative damage of cells[J]. Food Science,2020,41(13):160−168. doi: 10.7506/spkx1002-6630-20190714-183

    HE C B, DENG T, WEI H C, et al. Protective effect of polysaccharide from mesona blumes on oxidative damage of cells[J]. Food Science, 2020, 41(13): 160-168. doi: 10.7506/spkx1002-6630-20190714-183
    [11]
    随家宁, 李芳婵, 郭勇秀, 等. 三白草化学成分与药理作用研究进展及质量标志物预测分析[J]. 食品工业科技,2020,41(18):353−359,367. [SUI J N, LI F C, GUO Y X, et al. Research progress on chemical composition and pharmacological effects of Saururus chinensis and predictive analysis on quality marker[J]. Science and Technology of Food Industry,2020,41(18):353−359,367.

    SUI J N, LI F C, GUO Y X, et al. Research progress on chemical composition and pharmacological effects of Saururus chinensis and predictive analysis on quality marker[J]. Science and Technology of Food Industry, 2020, 41(18): 353-359, 367.
    [12]
    JEONG H J, KOO B S, KANG T H, et al. Inhibitory effects of Saururus chinensis and its components on stomach cancer cells[J]. Phytomedicine,2015,22(2):256−261. doi: 10.1016/j.phymed.2014.12.003
    [13]
    姜敏, 李露露, 吴桐, 等. 三白草酮通过调控海马Nrf2/HO -1表达减少酒精戒断大鼠抑郁样行为[J]. 中国病理生理杂志,2022,38(10):1803−1811. [JIANG M, LI L L, WU T, et al. Sauchinone attenuates alcohol withdrawal -induced depression like behaviors in rats by modulating hippocampal Nrf2/HO -1 expressionr[J]. Chinese Journal of Pathophysiology,2022,38(10):1803−1811.

    JIANG M, LI L L, WU T, et al. Sauchinone attenuates alcohol withdrawal-induced depression like behaviors in rats by modulating hippocampal Nrf2/HO-1 expressionr[J]. Chinese Journal of Pathophysiology, 2022, 38(10): 1803-1811.
    [14]
    吴丽娜, 范晓萌, 武爽, 等. 人参皂苷Rg1调节氧化应激和炎症因子表达改善糖尿病大鼠周围神经损伤[J]. 中国免疫学杂志,2021,37(4):486−491. [WU L N, FAN X M, WU S, et al. Ginsenoside Rg1 attenuates diabetic peripheral neuropathy in rats via anti-oxidant and anti inflammatory mechanisms[J]. Chinise J Immunology,2021,37(4):486−491.

    WU L N, FAN X M, WU S, et al. Ginsenoside Rg1 attenuates diabetic peripheral neuropathy in rats via anti-oxidant and anti inflammatory mechanisms[J]. Chinise J Immunology, 2021, 37(4): 486-491.
    [15]
    黄继汉, 黄晓晖, 陈志扬, 等. 药理试验中动物间和动物与人体间的等效剂量换算[J]. 中国临床药理学与治疗学,2004,9(9):1069−1072. [HUANG J H, HUANG X H, CHEN Z Y, et al. Dose conversion among different animals and healthy volunteers in pharmacological study[J]. Chin J Clin Pharmacol Ther,2004,9(9):1069−1072.

    HUANG J H, HUANG X H, CHEN Z Y, et al. Dose conversion among different animals and healthy volunteers in pharmacological study[J]. Chin J Clin Pharmacol Ther, 2004, 9(9): 1069-1072.
    [16]
    VELIKVA T V, KABAKCHIEVA P P, ASSYOV Y S, et al. Targeting inflammatory cytokines improve type 2 diabetes control[J]. Biomed Res Int,2021(2021):7297419.
    [17]
    ZHANG P, LI T, WU X, et al. Oxidatuve stress and diabetes: antioxidative strategies[J]. Front Med,2020,14(5):583−600. doi: 10.1007/s11684-019-0729-1
    [18]
    王宏杨, 苑天彤, 张福利. 中医药基于氧化应激途径防治糖尿病研究机制进展[J]. 辽宁中医药大学学报,2022,24(1):198−204. [WANG H Y, YUAN T T, ZHANG F L. Progress in research mechnism of traditional Chinese medicine in preventing and treating diabetes based on oxidative stress[J]. J Liaoning University of Traditional Chinese Medicine,2022,24(1):198−204.

    WANG H Y, YUAN T T, ZHANG F L. Progress in research mechnism of traditional Chinese medicine in preventing and treating diabetes based on oxidative stress[J]. J Liaoning University of Traditional Chinese Medicine, 2022, 24(1): 198-204.
    [19]
    LI H, WANG G, YU Y, et al. α-1, 2-Mannosidase MAN1C1 inhibits proliferation and invasion of clear cell renal cell car-cinoma[J]. J Cancer,2018,9(24):4618−4626. doi: 10.7150/jca.27673
    [20]
    HONG Y, HUANG X, AN L, et al. Overexpression of COPS3 promotes clear cell renal cell carcinoma progression via regulation of Phospho-AKT (Thr308), Cyclin D1 and Caspase-3[J]. Exp Cell Res,2018,365(2):163−170. doi: 10.1016/j.yexcr.2018.02.025
    [21]
    YANG S X, ZHANG Z C, BAI H L. ClC-5 alleviates renal fibrosis inunilateral ureteral obstruction mice[J]. Hum Cell,2019,32(3):297−305. doi: 10.1007/s13577-019-00253-5
    [22]
    高婷婷, 叶鸣, 吴凤瑶, 等. 二甲双胍对二型糖尿病大鼠肠道刺激影响[J]. 临床医药文献电子杂志,2019,6(23):178. [GAO T T, YE M, WU F Y, et al. Effect of metformin on intestinal stimulation in type 2 diabetic rats[J]. Journal of Clinical Medical Literature (Electronic Edition),2019,6(23):178.

    GAO T T, YE M, WU F Y, et al. Effect of metformin on intestinal stimulation in type 2 diabetic rats[J]. Journal of Clinical Medical Literature (Electronic Edition), 2019, 6(23): 178.
    [23]
    BO J S, XIE S Y, GUO Y, et al. Methylglyoxal impairs insulin secretion of pancreatic β-cells through increased production of ROS and mitochondrial dysfunction mediated by upregulation of UCP2 and MAPKs[J]. Journal of Diabetes Research, 2016: 2029854.
    [24]
    YANG M, KAN L, WU L, et al. Effect of baicalin on renal function in patients with diabetic nephropathy and its therapeutic mechanism[J]. Exp Ther Med,2019,17(3):2071−2076.
    [25]
    叶蕻芝, 许雪琴, 林薇, 等. 三白草对四氧嘧啶型糖尿病小鼠治疗作用的实验研究[J]. 福建中医学院学报,2004,14(3):34−35. [YE H Z, XU X Q, LIN W, et al. The study of Saururus on alloxan diabetic mice[J]. Journal of Fujian College of Traditional Medicine,2004,14(3):34−35.

    YE H Z, XU X Q, LIN W, et al. The study of Saururus on alloxan diabetic mice[J]. Journal of Fujian College of Traditional Medicine, 2004, 14(3): 34-35.
    [26]
    郭翔宇, 段颖, 李娟娥, 等. 三白草提取物对肥胖Zucker大鼠胰岛素抵抗的疗效观察[J]. 中华中医药杂志,2010,25(12):2291−2294. [GUO X Y, DUAN Y, LI J E, et al. Study on antidiabetic effects of Saururus Chinensis on obese Zucker rats[J]. CJTCMP,2010,25(12):2291−2294.

    GUO X Y, DUAN Y, LI J E, et al. Study on antidiabetic effects of Saururus Chinensis on obese Zucker rats[J]. CJTCMP, 2010, 25(12): 2291-2294.
    [27]
    LEE M S, ZHAO H X, LIU X M, et al. Protective effect of hydroxysafflor yellow a on nephropathy by attenuating oxidative stress and inhibiting apoptosis in induced type 2 diabetes in rat[J]. Oxid Med Cell Longev, 2020, 2020(1):1-11
    [28]
    LIAO Z Z, ZHANG J Y, WANG J Y, et al. The anti-nephritic activity of a polysaccharide from okra (Abelmoschus esculentus (L.) Moench) via modulation of AMPK-Sirt1-PGC-1α signaling axis mediated anti-oxidative in type 2 diabetes model mice[J]. Int J Biol Macromol,2019,140(11):568−576.
    [29]
    ROJAS J, BERMUDEZ V, PALMAR J, et al. Pancreatic beta cell death: Novel potential mechanisms in diabetes therapy[J]. J Diabetes Res,2018,2018:9601801.
    [30]
    张芸绮, 蔺晓菁, 王妙然, 等. 橙皮苷对高脂高糖诱导胰岛β细胞损伤的保护作用[J]. 中国药理学通报,2021,37(5):652−656. [ZHANG Y Q, LIN X J, WANG M R, et al. The protective effect of hesperidin on injury induced by glucolipotoxicity in islet beta cells[J]. Chinese Pharmacological Bulletin,2021,37(5):652−656.

    ZHANG Y Q, LIN X J, WANG M R, et al. The protective effect of hesperidin on injury induced by glucolipotoxicity in islet beta cells[J]. Chinese Pharmacological Bulletin, 2021, 37(5): 652-656.
    [31]
    MALIK S, SUCHAL K, KHAN SI, et al. Apigenin ameliorates streptozotocin induced diabetic nephropathy in rats via MAPK -NF-κB- TNF-α and TGF-β1-MAPK-fibronectin pathways[J]. Am J Physiol Renal Physiol,2017,313(2):F414−F422. doi: 10.1152/ajprenal.00393.2016
    [32]
    JI L, CHEN Y, WANG H, et al. Overexpression of Sirt6 promotes M2 macrophage transformation, alleviating renal injury in diabetic nephropathy[J]. Int J Oncol,2019,55(1):103−115.
    [33]
    YANG P Y, LI P C, FENG B. Protective effects of gliclazide on high glucose and AGEs induced damage of glomerular mesangial cells and renal tubular epithelial cells via inhibiting RAGE -p22phox -NF -kB pathway[J]. Eur Rev Med Pharmacol Sci,2019,23(20):9099−9107.
    [34]
    ALAKLABIA, ARIFIA, AHAMED A, et al. Evaluation of antioxidant and anticancer activities of chemical constituents of the saururus chinensis root extracts[J]. Saudi Journal of Biological Sciences,2018,25(7):1387−1392. doi: 10.1016/j.sjbs.2016.12.021
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