Optimization of Ultrasonic-Assisted Deep Eutectic Solvent Extraction Process, Biological Activities and Components of Burdock Polyphenols

WU Yonghua; WANG Jieyu; HUANG Lili; PAN Sicen; ZHANG Jianping; LIU Enqi

doi:10.13386/j.issn1002-0306.2022110252

Volume 44 Issue 21

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(21): 171-179. > DOI: 10.13386/j.issn1002-0306.2022110252

WU Yonghua, WANG Jieyu, HUANG Lili, et al. Optimization of Ultrasonic-Assisted Deep Eutectic Solvent Extraction Process, Biological Activities and Components of Burdock Polyphenols[J]. Science and Technology of Food Industry, 2023, 44(21): 171−179. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110252.

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Optimization of Ultrasonic-Assisted Deep Eutectic Solvent Extraction Process, Biological Activities and Components of Burdock Polyphenols

Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou 221018, China

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Received Date: November 23, 2022
Available Online: September 06, 2023

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Abstract

Abstract

The extraction effects of six deep eutectic solvents on burdock polyphenols (BPs) were investigated, and the response surface methodology was used to optimize the process conditions of ultrasonic-assisted deep eutectic solvent extraction of BPs, moreover, the total reducing power, the DPPH• scavenging capacity, the inhibition of α-glucosidase and cholic acid binding capacity in vitro were studied, and its composition were analyzed by HPLC. The results showed that DES 5 (the molar ratio of choline chloride to ethylene glycol was 1:4) was the best solvent system for the extraction of BPs. The optimum technological conditions were as follows, the water content of deep eutectic solvent was 40%, the ratio of material to liquid was 55:1 mg/mL, the extraction temperature was 49 ℃, and the extraction time was 41 min, under the above conditions, the yield of BPs was 1.19%±0.11%. The biological activity analysis showed that the IC₅₀ of the total reducing power and DPPH• scavenging capacity of burdock polyphenol were 0.93 mg/mL and 7.03 μg/mL, respectively, indicating that it had good antioxidant capacity, but weaker than V_C. The IC₅₀ of α-glucosidase inhibition was 0.13 mg/mL, which was higher than acarbose, indicating that it possessed excellent hypoglycemic activity. In addition, its cholic acid binding rate was 33.23% when the mass concentration was 1.5 mg/mL, implied that it had a certain hypolipidemic activity. HPLC analysis showed that BPs contained chlorogenic acid, caffeic acid and ferulic acid. The results would provide a theoretical basis for the green preparation of burdock polyphenol and its development and utilization as a functional component of antioxidation, hypoglycemia and blood lipids.
- deep eutectic solvent,
- burdock,
- polyphenols,
- biological activity,
- component analysis

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