Effects of Phenyllactic Acid on <i>Listeria monocytogenes</i> Biofilms

JIANG Congyi; KANG Rui; YU Tao; JIANG Xiaobing

doi:10.13386/j.issn1002-0306.2022100239

Volume 44 Issue 18

Sep. 2023

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Science and Technology of Food Industry > 2023 > 44(18): 164-172. > DOI: 10.13386/j.issn1002-0306.2022100239

JIANG Congyi, KANG Rui, YU Tao, et al. Effects of Phenyllactic Acid on Listeria monocytogenes Biofilms[J]. Science and Technology of Food Industry, 2023, 44(18): 164−172. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100239.

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Effects of Phenyllactic Acid on Listeria monocytogenes Biofilms

1.
College of Life Sciences, Henan Normal University, Xinxiang 453007, China
2.
School of Life Sciences & Basic Medicine, Xinxiang University, Xinxiang 453000, China

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Received Date: October 23, 2022
Available Online: July 13, 2023

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Abstract

Abstract

To investigate the effect of phenyllactic acid (PLA) on Listeria monocytogenes biofilms, crystal violet method was used to measure the biomass of L. monocytogenes biofilms, and inverted microscope and scanning electron microscope were used to observe the structure of L. monocytogenes biofilms, the content of extracellular polysaccharide, extracellular protein and extracellular DNA in biofilm was measured by phenol-sulfuric acid method, Folin-phenol method and diphenylamine method, respectively. The effect of PLA on the transcription of genes associated with biofilm formation of L. monocytogenes was detected by real-time quantitative PCR. The results showed that the minimum inhibitory concentration values of PLA against eight L. monocytogenes strains were 6 mg/mL. PLA inhibited L. monocytogenes biofilm formation by reducing the synthesis of extracellular polysaccharides and extracellular proteins. For mature biofilms of L. monocytogenes, PLA destroyed the biofilm structure and reduced the biofilm biomass by reducing the amount of extracellular polysaccharides and extracellular proteins. PLA also inhibited the swarming motility of L. monocytogenes. The transcription levels of quorum sensing system agr in L. monocytogenes were significantly (P<0.05) decreased in the presence of PLA. As a natural antimicrobial compound, PLA had the potential application to control food contamination by L. monocytogenes and its biofilms.
- Listeria monocytogenes,
- phenyllactic acid,
- biofilm,
- extracellular polymeric substances

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