Ultrasound-Assisted Extraction and Analysis of Antioxidation and Hypolipidemia Activities of Polysaccharides from Lilium brownii var. viridulum

WANG Weizhen; YANG Panpan; TI Yongrui; TANG Yuchao; XU Leifeng; WU Xuewei; SONG Zihan; MING Jun

doi:10.13386/j.issn1002-0306.2022100237

Volume 44 Issue 18

Sep. 2023

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Science and Technology of Food Industry > 2023 > 44(18): 251-257. > DOI: 10.13386/j.issn1002-0306.2022100237

WANG Weizhen, YANG Panpan, TI Yongrui, et al. Ultrasound-Assisted Extraction and Analysis of Antioxidation and Hypolipidemia Activities of Polysaccharides from Lilium brownii var. viridulum[J]. Science and Technology of Food Industry, 2023, 44(18): 251−257. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100237.

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Ultrasound-Assisted Extraction and Analysis of Antioxidation and Hypolipidemia Activities of Polysaccharides from Lilium brownii var. viridulum

1.
School of Agriculture, Yunnan University, Kunming 650091, China
2.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

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Received Date: October 23, 2022
Available Online: July 12, 2023

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Abstract

Abstract

Lilium brownii var. viridulum is one of the main traditional edible and medicinal lily in China. This study aimed to explore the extraction technology, physicochemical properties and functional activity of polysaccharide from Lilium brownii var. viridulum, used Lilium brownii var. viridulum scales as materials, polysaccharide yield as the index, solid-liquid ratio, extraction time and extraction temperature as factors to optimize the extraction process of lily polysaccharide. Then the polysaccharide was purified through removing protein. The physicochemical properties of the polysaccharides were analyzed, and the antioxidant and lipid-lowering abilities were measured in vitro. The results showed that the optimal extraction conditions were the material to liquid ratio 1:20 g/mL, extraction time 20 min, extraction temperature 75℃, and polysaccharide yield was 11.98%. The contents of total sugar, uronic acid and protein of crude polysaccharide were 58.46%, 8.06% and 10.85%, respectively. After deproteinization, the contents of total sugar and uronic acid of polysaccharide were increased to 84.78% and 15.41%, respectively, and the content of protein was reduced to 4.73%. The scavenging rates of 10 mg/mL deproteinized polysaccharide for DPPH and ABTS⁺ free radicals were 8.75% and 38.59%, respectively, which were lower than those of control V_C. The inhibition rate of deproteinized polysaccharide for pancreatic lipase was 85.78%, which was higher than that of the control orlistat, indicating good inhibit pancreatic lipase effect in vitro. These results would provide a basis for further study on the structure-activity relationship and development of potential functional food, and provide theoretical reference for the application of lily.
- Lilium brownii var. viridulum,
- polysaccharide,
- orhogonal design,
- antioxidant activity,
- pancreatic lipase

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[1]	中国科学院中国植物志编辑委员会. 中国植物志: 第14卷[M]. 北京: 科学出版社, 1980: 157−166 Editorial Committee of Flora of China, Chinese Academy of Sciences. Flora of China: Volume 14[M]. Beijing: Science Press, 1980: 166−157.
[2]	国家药典委员会. 中华人民共和国药典[M].2010年版. 一部. 北京: 中国医药科技出版社, 2010 Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia[M]. 2010. Part I. Beijing: China Medical Science Press, 2010
[3]	李玉帆, 明军, 刘新艳, 等. 15个百合种和品种的食用性比较研究[C]// 中国园艺学会2013年学术年会论文摘要集[A]. 成都: 园艺学报, 2013: 124 LI Y F, MING J, LIU X Y, et al. Comparative study on edible applicability of 15 lily species and varieties[C]// Collection of abstracts from 2013 annual conference papers of Chinese Society for Horticultural Science[A]. Chengdu: Acta Horticulturae Sinica, 2013: 124.
[4]	袁泽玥, 张典, 崔迎春. 百合多糖的药理作用研究进展[J]. 科学技术创新,2020(15):25−26. [YUAN Z Y, ZHANG D, CUI Y C. Research progress on pharmacological effects of lily polysaccharide[J]. Scientific and Technological Innovation,2020(15):25−26. YUAN Z Y, ZHANG D, CUI Y C. Research progress on pharmacological effects of lily polysaccharide[J]. Scientific and technological innovation, 2020(15): 25-26.
[5]	赵祥杰, 杨荣玲, 邝哲师, 等. 植物来源多糖的研究进展[J]. 安徽农业科学,2012,40(35):17016−17018. [ZHAO X J, YANG R L, CUI Y C, et al. Research progress of polysaccharides derived from plants[J]. Journal of Anhui Agri,2012,40(35):17016−17018. Zhao X J, Yang R L, Cui Y C, et al. Research progress of polysaccharides derived from plants[J]. Journal of Anhui Agri. 2012, 40(35): 17016-17018
[6]	KOPELMAN P G. Obesity as a medical problem[J]. Nature,2000,404(6778):635−643. doi: 10.1038/35007508
[7]	ALLISON D B, NEALE M C, KEZIS M I, et al. Assortative mating for relative weight: Genetic implications[J]. Behavior Genetics,1996,26(2):103−111. doi: 10.1007/BF02359888
[8]	HARIRI N, THIBAULT L. High-fat diet-induced obesity in animal models[J]. Nutrition Research Reviews,2010,23(2):270−299. doi: 10.1017/S0954422410000168
[9]	SEYEDAN A, ABDULLAH M A, ALSHAGGA M A, et al. Medicinal plants and their inhibitory activities against pancreatic lipase: A review[J]. Evidence-Based Complementary and Alternative Medicine, 2015: 1-13.
[10]	WEIBEL E K, HADBARY P, HOCHULI E, et al. Lipstatin, an inhibitor of pancreatic lipase, produced by Streptomyces toxytricini. II. Chemistry and structure elucidation[J]. Journal of Antibiotics,1987,40(8):1081−1085. doi: 10.7164/antibiotics.40.1081
[11]	TISS A, LENGSFELD H, CARRIÈRE F, et al. Inhibition of human pancreatic lipase by tetrahydrolipstatin: Further kinetic studies showing its reversibility[J]. Journal of Molecular Catalysis B Enzymatic,2009,58(1-4):41−47. doi: 10.1016/j.molcatb.2008.11.003
[12]	VINER R, HSIA Y, TOMSIC T, et al. Efficacy and safety of anti-obesity drugs in children and adolescents: Systematic review and meta-analysis: Obesity management[J]. Obesity Reviews An Official Journal of the International Association for the Study of Obesity,2010,11(8):593−602.
[13]	FILIPPATOS T D, DERDEMEZIS C S, GAZI I F, et al. Orlistat-associated adverse effects and drug interactions: A critical review[J]. Drug Safety,2008,31(1):53−65. doi: 10.2165/00002018-200831010-00005
[14]	李鑫鑫, 王一棠, 陈琛, 等. 12种植物粗多糖的体外抗肥胖及抗氧化活性研究[J]. 食品科技,2018,43(2):222−227. [LI X X, WAGN Y T, CHEN S, et al. The anti-obesity and anti-oxidant activities of 12 crude polysaccharide[J]. Food Science and Technology,2018,43(2):222−227. LI X X, WAGN Y T, CHEN S, et al. The anti-obesity and anti-oxidant activities of 12 crude polysaccharide[J]. Food Science and Technology, 2018, 43(2): 222-227.
[15]	ZHOU X, WANG D, SUN P, et al. Effects of soluble tea polysaccharides on hyperglycemia in alloxan-diabetic mice[J]. Journal of Agricultural & Food Chemistry,2007,55(14):5523−5528.
[16]	ZHANG J, WEN C, WEI Q, et al. Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes[J]. International Journal of Biological Macromolecules,2018,118:2269−2277. doi: 10.1016/j.ijbiomac.2018.07.098
[17]	CHEN G, FANG C, RAN C X, et al. Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products[J]. International Journal of Biological Macromolecules,2019,130:903−914. doi: 10.1016/j.ijbiomac.2019.03.038
[18]	王荣琨, 王钦, 罗欣, 等. 不同提取方法对竹荪多糖提取率及抗氧化性影响[J]. 中国食用菌,2020,39(3):24−28. [WANG R K, WAGN Q, LUO X, et al. Effects of different extraction methods on the extraction rate and antioxidant properties of dictyophora indusiata polysaccharide[J]. Edible Fungi of China,2020,39(3):24−28. WANG R K, WAGN Q, LUO X, et al. Effects of different extraction methods on the extraction rate and antioxidant properties of dictyophora indusiata polysaccharide[J]. Edible Fungi of China, 2020, 39(3): 24-28.
[19]	马高兴, 王晗, 杨文建, 等. 不同提取工艺对杏鲍菇多糖结构特征及免疫活性的影响[J]. 食品科学,2022,43(17):42−49. [MA G X, WANG H, YANG W J, et al. Effects different extraction processes on structural characteristic and immunomodulatory activity of pleurotus eryngii polysaccharide[J]. Food Science,2022,43(17):42−49. MA G X, WANG H, YANG W J, et al. Effects different extraction processes on structural characteristic and immunomodulatory activity of pleurotus eryngii polysaccharide[J]. Food Science, 2022, 43(17): 42-49.
[20]	钟敏. 百合多糖的提纯、结构表征及免疫调节作用研究[D]. 北京: 北京林业大学, 2021 ZHOGN M. Extraction, purification, structure characterization and immunomodulatory activity of polysaccharides from edible lily[D]. Beijing: Beijing Forestry University, 2021.
[21]	陈小蒙. 水溶性龙牙百合多糖的纯化、一级结构及其生物活性研究[D]. 南昌: 南昌大学, 2008 CHEN X M. Study on the purification and primary structure of water-soluble longya lily polysaccharide and its biological activities[D]. Nanchang: Nanchang University, 2008.
[22]	唐明. 百合多糖的提取、纯化及抑菌活性研究[D]. 长沙: 湖南农业大学, 2010 TANG M. Research on extraction, purification and bacteriostatic activity of polysaccharides in Lilium[D]. Changsha: Hunan Agricultural University, 2010.
[23]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 15672-2009 食用菌中总糖含量的测定[S]. 北京: 中国标准出版社, 2009 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China. GB/T 15672-2009 Determination of total sugar content in edible fungi[S]. Beijing: Standards Press of China, 2009.
[24]	MEYER A S, ISAKSEN A. Application of enzymes as food antioxidants[J]. Trends in Food Science & Technology,1995,6(9):300−304.
[25]	何执中, 金瓯, 何执静. 介绍一种胰脂肪酶的简便测定方法[J]. 生化药物杂志,1991(2):79−81. [HE Z Z, JIN O, HE Z J. A simple method for the determination of pancreatic lipase was introduced[J]. Chinese Journal of Biochemical Pharmaceutics,1991(2):79−81. HE Z Z, JIN O, HE Z J. A simple method for the determination of pancreatic lipase was introduced[J]. Chinese Journal of Biochemical Pharmaceutics, 1991(2): 79-81.
[26]	杨文雅, 李长征, 张海晖, 等. 蛹虫草多糖的亚临界水萃取及其抗氧化活性研究[J]. 食品工业科技,2016,37(5):252−257. [YANG W Y, LI C Z, ZHAGN H H, et al. Study on the optimization for the extraction and antioxidant activity of polysaccharide from cordyceps militaris by subcritical water[J]. Science and Technology of Food Industry,2016,37(5):252−257. YANG W Y, LI C Z, ZHAGN H H, et al. Study on the optimization for the extraction and antioxidant activity of polysaccharide from cordyceps militaris by subcritical water[J]. Science and Technology of Food Industry, 2016, 37(5): 252-257.
[27]	秦德利, 贾坤, 窦珺荣, 等. 茶树花多糖超声波辅助热水浸提工艺优化[J]. 食品工业科技,2015,36(4):215−218,223. [QIN D L, JIA K, DOU Q R, et al. Optimization of ultrasound-assisted hot water extraction of polysaccharides from tea flower[J]. Science and Technology of Food Industry,2015,36(4):215−218,223. QIN D L, JIA K, DOU Q R, et al. Optimization of ultrasound-assisted hot water extraction of polysaccharides from tea flower[J]. Science and Technology of Food Industry, 2015, 36(4): 215-218+223.
[28]	MING M, BAI A, BO J, et al. Characterisation of a novel water-soluble polysaccharide from Leuconostoc citreum SK24.002[J]. Food Hydrocolloids,2014,36(4):265−272.
[29]	ZHANG Z, WANG X, MO X, et al. Degradation and the antioxidant activity of polysaccharide from Enteromorpha linza[J]. Carbohydrate Polymers,2013,92(2):2084−2087. doi: 10.1016/j.carbpol.2012.11.096
[30]	曾桥, 韦承伯, 韩国锋, 等. 桑叶茯砖茶多糖的响应面提取工艺优化及其体外抗氧化降血脂作用[J]. 食品工业科技,2018,39(18):193−200. [ZENG Q, WEI C B, HAN G F, et al. Optimization of polysaccharides extraction from mulberry leaves fu brick tea by response surface methodology and its antioxidant activities & hypolipidemic effects in vitro[J]. Science and Technology of Food Industry,2018,39(18):193−200. ZENG Q, WEI C B, HAN G F, et al. Optimization of polysaccharides extraction from mulberry leaves fu brick tea by response surface methodology and its antioxidant activities & hypolipidemic effects in vitro[J]. Science and Technology of Food Industry, 2018, 39(18): 193-200.
[31]	钟丽霞, 江震宇, 汪嘉妮, 等. 山楂多糖提取工艺优化及其降血糖、降血脂活性[J]. 食品工业科技,2019,40(13):119−124,147. [ZHONG L X, JIANG Z Y, WAGN J N, et al. Optimization of extraction technology of hawthorn polysaccharides and its hypoglycemic and hypolipidemic activity[J]. Science and Technology of Food Industry,2019,40(13):119−124,147. ZHONG L X, JIANG Z Y, WAGN J N, et al. Optimization of extraction technology of hawthorn polysaccharides and its hypoglycemic and hypolipidemic activity[J]. Science and Technology of Food Industry, 2019, 40(13): 119-124+147.
[32]	余倩莎. 龙牙百合多糖提取物抗氧化活性及其对肿瘤细胞生长影响[D]. 长沙: 湖南农业大学, 2017 YU Q S. Lilium polysaccharide extract antioxidant activity and its effect on tumor cell. Changsha: Hunan Agricultural University, 2017.
[33]	LI Q, YU N, WANG Y, et al. Extraction optimization of Bruguiera gymnorrhiza polysaccharides with radical scavenging activities[J]. Carbohydrate Polymers,2013,96(1):148−155. doi: 10.1016/j.carbpol.2013.03.054
[34]	朱泉. 百合多糖分离纯化、结构鉴定及其生物活性研究[D]. 南京: 南京农业大学, 2012 ZHU Q. Isolation, purification, structure determination and biological activities of polysaccharides from bulb of lily (Lilium Lancifolium Thunb.)[D]. Nanjing: Nanjing Agricultural University, 2012.
[35]	WANG X, ZHANG Z, YAO Q, et al. Phosphorylation of low-molecular-weight polysaccharide from Enteromorpha linza with antioxidant activity[J]. Carbohydrate Polymers,2013,96(2):371−375. doi: 10.1016/j.carbpol.2013.04.029
[36]	黄菊青. 竹茹多糖的化学结构和免疫活性研究[D]. 杭州: 浙江大学, 2015 HAUNG J Q. Chemical characterization and immunomodulatory activity of polysaccharides from bamboo shavings[D]. Hangzhou: Zhejiang University, 2015.
[37]	李化强, 吴菲菲, 龙艳珍, 等. 隆回龙牙百合多糖的体外抗氧化活性研究[J]. 食品安全质量检测学报,2017,8(8):3068−3073. [LI H Q, WU F F, LONG Y Z, et al. In vitro antioxidative effects of Longhui Longya lilium polysaccharides[J]. Journal of Food Safety and Quality,2017,8(8):3068−3073. doi: 10.3969/j.issn.2095-0381.2017.08.038 LI H Q, WU F F, LONG Y Z, et al. In vitro antioxidative effects of Longhui Longya lilium polysaccharides[J]. Journal of Food Safety and Quality, 2017, 8(8): 3068-3073. doi: 10.3969/j.issn.2095-0381.2017.08.038
[38]	冉琳, 何钢, 梁立, 等. 黑木耳多糖对胰脂肪酶活性的抑制作用[J]. 食品工业科技,2017,38(22):56−60. [RAN L, HE G, LAIGN L, et al. Inhibitory effect of Auricularia auricula polysaccharides on pancreatic lipase activity[J]. Science and Technology of Food Industry,2017,38(22):56−60. RAN L, HE G, LAIGN L, et al. Inhibitory effect of Auricularia auricula polysaccharides on pancreatic lipase activity[J]. Science and Technology of Food Industry, 2017, 38(22): 56-60.
[39]	高航, 吴楠, 高延芬, 等. 莲子红衣多糖对胰脂肪酶活性抑制作用的研究[J]. 粮食与油脂,2017,30(5):87−91. [GAO H, WU N, GAO Y F, et al. Inhibitory effect on pancreatic lipase activity of polysaccharide from lotus seed skin[J]. Cereals & Oils,2017,30(5):87−91. GAO H, WU N, GAO Y F, et al. Inhibitory effect on pancreatic lipase activity of polysaccharide from lotus seed skin[J]. Cereals & Oils, 2017, 30(5): 87-91.

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