LU Huiqin, MEI Yiwen, XIE Qilan, et al. Effects of L-Lys on the Quality Characteristics and Oxidation Degree of Yak Meat under MDA Induced Oxidation[J]. Science and Technology of Food Industry, 2023, 44(16): 90−98. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100175.
Citation: LU Huiqin, MEI Yiwen, XIE Qilan, et al. Effects of L-Lys on the Quality Characteristics and Oxidation Degree of Yak Meat under MDA Induced Oxidation[J]. Science and Technology of Food Industry, 2023, 44(16): 90−98. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100175.

Effects of L-Lys on the Quality Characteristics and Oxidation Degree of Yak Meat under MDA Induced Oxidation

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  • Received Date: October 18, 2022
  • Available Online: June 07, 2023
  • In order to study the effects of L-Lys on the quality characteristics and protein oxidation of yak meat induced by MDA. In this study, MDA was used as the simulated oxidation system, and yak meat were used as the research objects. The changes of edible quality, lipid oxidation, protein oxidation and muscle histological characteristics during storage were measured after yak meat treated with different concentrations of L-Lys and MDA. The results showed that 10 mmol/L L-Lys could reduce the increase of pH caused by MDA, alleviate the excessive increase of hardness and chewiness, and significantly inhibit the conversion of oxygenated myoglobin into high iron myoglobin, thereby increasing the L* value and a* value (P<0.05), so as to maintain good color. In addition, L-Lys had a significant inhibitory effect on MDA by inducing lipid and protein oxidation, which was manifested by the decrease of POV and TBARS (P<0.05), and the significant increase of sulfhydryl and active sulfhydryl (P<0.05). L-Lys of 10 mmol/L could also significantly inhibit protein carbonylation (P<0.05) in the early stage of storage. Meanwhile, L-Lys could maintain the integrity of muscle fiber structure and reduce MFI to a certain extent. The above results indicated that L-Lys could delay MDA induced lipid and protein oxidation in meat to a certain extent through concentration dependence, thereby improving the oxidative stability of meat and reducing the deterioration of meat quality. In general, L-Lys at 10 mmol/L had a better effect. Therefore, L-Lys was proved to have a potential application value in inhibiting the oxidation of yak meat products and improving their edible quality.
  • [1]
    王兆明, 贺稚非, 李洪军. 脂质和蛋白质氧化对肉品品质影响及交互氧化机制研究进展[J]. 食品科学,2018,39(11):295−301. [WANG Z M, HE Z F, LI H J. A review of the effect of lipid and protein oxidation on meat quality and their interrelationship[J]. Food Science,2018,39(11):295−301. doi: 10.7506/spkx1002-6630-201811045

    WANG Z M, HE Z F, LI H J. A review of the effect of lipid and protein oxidation on meat quality and their interrelationship[J]. Food Science, 2018, 39(11): 295-301. doi: 10.7506/spkx1002-6630-201811045
    [2]
    周非白. 氧化修饰对猪肉肌原纤维蛋白结构与功能特性的调控研究[D]. 广州: 华南理工大学, 2016

    ZHOU F B. Oxidative modification on porcine myofibrillar protein in relation to structural changes and functional properties[D]. Guangzhou: South China University of Technology, 2016.
    [3]
    陈腊梅, 唐善虎, 李思宁, 等. 丙二醛氧化对牦牛肉肌原纤维蛋白结构及功能特性的影响[J]. 食品科学,2023,44(8):46−54. [CHEN L M, TANG S H, LI S N, et al. Effects of malondialdehyde oxidation on the structure and functional properties of myofibrillar proteins in yak meat[J]. Food Science,2023,44(8):46−54. doi: 10.7506/spkx1002-6630-20220627-299

    CHEN L M, TANG S H, LI S N, et al. Effects of malondialdehyde oxidation on the structure and functional properties of myofibrillar proteins in yak meat[J]. Food Science, 2023, 44(8): 46-54. doi: 10.7506/spkx1002-6630-20220627-299
    [4]
    郭亚晶, 刘昆仑. 脂质氧化产物-蛋白质相互作用研究进展[J]. 食品安全质量检测学报,2021,12(13):5327−5332. [GUO Y J, LIU K L. Research progress of interaction between lipid oxidation product and protein[J]. Journal of Food Safety and Quality,2021,12(13):5327−5332.

    GUO Y J, LIU K L. Research progress of interaction between lipid oxidation product and protein[J]. Journal of Food Safety and Quality, 2021, 12: 5327-5332.
    [5]
    WANG Z, HE Z, EMARA A M, et al. Effects of malondialdehyde as a byproduct of lipid oxidation on protein oxidation in rabbit meat[J]. Food Chemistry,2019,288:405−412. doi: 10.1016/j.foodchem.2019.02.126
    [6]
    ZHOU C, LI J, TAN S. Effect of l-lysine on the physicochemical properties of pork sausage[J]. Food Science and Biotechnology,2014,23(3):775−780. doi: 10.1007/s10068-014-0104-6
    [7]
    HWANG H S, WINKLER-MOSER J K. Antioxidant activity of amino acids in soybean oil at frying temperature: Structural effects and synergism with tocopherols[J]. Food Chemistry,2017,221:1168−1177. doi: 10.1016/j.foodchem.2016.11.042
    [8]
    ZHANG D, XU B, ZHANG Y, et al. l-Arginine/l-lysine alleviated the deterioration of emulsion sausages with partial replacement of porcine backfat by soybean oil by hindering hydroxyl radical stressed oxidation of meat proteins[J]. ACS Food Science & Technology,2021,1(5):967−974.
    [9]
    CAO Y G, LI B L, FAN X, et al. Synergistic recovery and enhancement of gelling properties of oxidatively damaged myofibrillar protein by L-lysine and transglutaminase[J]. Food Chemistry,2021,358:129860. doi: 10.1016/j.foodchem.2021.129860
    [10]
    WANG L, ZHANG M, BHANDARI B, et al. Effects of malondialdehyde-induced protein modification on water functionality and physicochemical state of fish myofibrillar protein gel[J]. Food Research International,2016,86:131−139. doi: 10.1016/j.foodres.2016.06.007
    [11]
    KRZYWICKI. The determination of haem pigments in meat[J]. Meat Science,1982,7(1):29−36. doi: 10.1016/0309-1740(82)90095-X
    [12]
    李文博, 罗玉龙, 郭月英, 等. 苏尼特羊宰后成熟过程中肌原纤维蛋白特性与肉品质的变化分析[J]. 食品科学,2021,42(3):48−55. [LI W B, LUO Y L, GUO Y Y, et al. Analysis of changes in myofibrillar protein properties and meat quality during post-mortem aging of Sunit sheep carcasses[J]. Food Science,2021,42(3):48−55. doi: 10.7506/spkx1002-6630-20191225-299

    LI W B, LUO Y L, GUO Y Y, et al. Analysis of changes in myofibrillar protein properties and meat quality during post-mortem aging of Sunit sheep carcasses[J]. Food Science, 2021, 42(3): 48-55. doi: 10.7506/spkx1002-6630-20191225-299
    [13]
    毛云, 周昱宇, 刘静雅, 等. 不同种类脂肪替代物对牦牛肉糜凝胶品质特性影响的研究[J]. 食品与发酵工业, 2023, 49(6): 227−234

    MAO Y, ZHOU Y Y, LIU J Y, et al. Effects of different kinds of fat substitutes on gel quality characteristics of yak meat surimi[J]. Food and Fermentation Industries, 2023, 49(6): 227−234.
    [14]
    姚峥. 复合盐对菊花猪肉香肠脂质氧化特性和品质特性的影响[D]. 合肥: 合肥工业大学, 2013

    YAO Z. Effects of compound salt on fat oxidation and qualities of pork sausage with chrysanthemum[D]. Hefei: Hefei University of Technology, 2013
    [15]
    JONGBERG S, TORNGREN M A, GUNVIG A, et al. Effect of green tea or rosemary extract on protein oxidation in Bologna type sausages prepared from oxidatively stressed pork[J]. Meat Science,2013,93(3):538−546. doi: 10.1016/j.meatsci.2012.11.005
    [16]
    HUANG X, SUN L, LIU L, et al. Study on the mechanism of mulberry polyphenols inhibiting oxidation of beef myofibrillar protein[J]. Food Chemistry,2022,372:131241. doi: 10.1016/j.foodchem.2021.131241
    [17]
    瞿丞, 贺稚非, 王兆明, 等. 不同食盐添加量腌制对鸡肉脂质氧化、蛋白质氧化及食用品质的影响[J]. 食品科学,2020,41(16):77−85. [QU C, HE Z F, WANG Z M, et al. Effects of different salt concentrations on lipid oxidation, protein oxidation and eating quality of cured chicken meat[J]. Food Science,2020,41(16):77−85.

    QU C, HE Z F, WANG Z M, et al. Effects of different salt concentrations on lipid oxidation, protein oxidation and eating quality of cured chicken meat[J]. Food Science, 2020, 41(16): 77-85.
    [18]
    吴盈茹, 廖月琴, 杜琪, 等. 盐浓度对冷藏即食中华管鞭虾肌肉品质的影响[J]. 食品工业科技,2023,44(2):90−97. [WU Y R, LIAO Y Q, DU Q, et al. Effect of salt concentration on muscle quality of ready-to-eat shrimp (Solenocera crassicornis) during chilled storage[J]. Science and Technology of Food Industry,2023,44(2):90−97.

    WU Y R, LIAO Y Q, DU Q, et al. Effect of salt concentration on muscle quality of ready-to-eat shrimp (Solenocera crassicornis) during chilled storage[J]. Science and Technology of Food Industry, 2023, 44(2): 90-97
    [19]
    刘彩华, 朱新荣, 张建. 羟自由基氧化对冷藏高白鲑品质及微观结构的影响[J]. 食品工业科技,2017,38(23):238−244. [LIU C H, ZHU X R, ZHANG J. Effects of protein oxidation on qualities and microstructure in refrigerated Coregonus peled muscle exposed to a hydroxyl radical-generating system[J]. Science and Technology of Food Industry,2017,38(23):238−244.

    LIU C H, ZHU X R, ZHANG J. Effects of protein oxidation on qualities and microstructure in refrigerated Coregonus peled muscle exposed to a hydroxyl radical-generating system[J]. Science and Technology of Food Industry, 2017, 38(23): 238-244.
    [20]
    LIU Y, YANG Y, LI B, et al. Effects of lipids with different oxidation levels on protein degradation and biogenic amines formation in Sichuan-style sausages[J]. LWT,2022,161:113344. doi: 10.1016/j.lwt.2022.113344
    [21]
    LI C, PENGQI B, YAN W, et al. Improving quality attributes of refrigerated prepared pork chops by injecting L-arginine and L-lysine solution[J]. LWT, 2022, 153.
    [22]
    陈蒙. 碱性氨基酸赖氨酸、精氨酸辅助微波对大肠杆菌的致死作用研究[D]. 无锡: 江南大学, 2017

    CHEN M. Study on the lethal effect of basic amino acids lysine and arginine assisted microwave on Escherichia coli[D]. Wuxi: Jiangnan University, 2017.
    [23]
    MAHMUDUL H M, VIPASHA S, CHYNGYZ E, et al. Principal component analysis of lipid and protein oxidation products and their impact on color stability in bison longissimus lumborum and psoas major muscles[J]. Meat Science,2021,178:108523. doi: 10.1016/j.meatsci.2021.108523
    [24]
    陈骋, 余群力, 韩玲, 等. 丙二醛对牛肉线粒体高铁肌红蛋白还原能力的影响[J]. 农业机械学报,2015,46(12):253−259. [CHEN C, YU Q L, HAN L, et al. Effects of malondialdehyde on metmyoglobin reduction ability of bovine muscle mitochondria[J]. Transactions of the Chinese Society for Agricultural Machinery,2015,46(12):253−259. doi: 10.6041/j.issn.1000-1298.2015.12.034

    CHEN C, YU Q L, HAN L, et al. Effects of malondialdehyde on metmyoglobin reduction ability of bovine muscle mitochondria[J]. Transactions of the Chinese Society for Agricultural Machinery, 2015, 46(12): 253-259 doi: 10.6041/j.issn.1000-1298.2015.12.034
    [25]
    齐婷婷, 张一敏, 杨啸吟, 等. 氧化应激对牛肉肉色及其稳定性的影响研究进展[J]. 食品科学,2023,44(7):260−266. [QI T T, ZHANG Y M, YANG X Y, et al. Recent progress in research on the effects of oxidative stress on beef color and stability[J]. Food Science,2023,44(7):260−266. doi: 10.7506/spkx1002-6630-20220516-213

    QI T T, ZHANG Y M, YANG X Y, et al. Recent Progress in Research on the Effects of Oxidative Stress on beef color and stability[J]. Food Science, 2023, 44(7): 260-266. doi: 10.7506/spkx1002-6630-20220516-213
    [26]
    ZHENG Y, XU P, LI S, et al. Effects of L-lysine/L-arginine on the physicochemical properties and quality of sodium-reduced and phosphate-free pork sausage[J]. International Journal of Nutrition and Food Sciences,2017,6(1):12. doi: 10.11648/j.ijnfs.20170601.13
    [27]
    NING C, BAO P, ZHANG D, et al. Reduction and coordination properties of L-lysine/ L-arginine/ L-cysteine for the improvement of the color of cured sausage[J]. Food Chemistry,2020,312(C):126122.
    [28]
    蒋祎人, 李涛, 刘友明, 等. 丙二醛氧化修饰对白鲢肌原纤维蛋白结构性质的影响[J]. 食品科学,2020,41(6):1−7. [JIANG Y R, LI T, LIU Y M, et al. Effect of malondialdehyde-induced protein oxidation on structural properties of myofifibrillar protein from silver carp (Hypophthalmichthys molitrix)[J]. Food Science,2020,41(6):1−7.

    JIANG Y R, LI T, LIU Y M, et al. Effect of malondialdehyde-induced protein oxidation on structural properties of myofifibrillar protein from silver carp (Hypophthalmichthys molitrix)[J]. Food Science, 2020, 41(6): 1-7.
    [29]
    LI X, WANG W, WANG S, et al. The Solubility and structures of porcine myofibrillar proteins under low-salt processing conditions as affected by the presence of L-lysine[J]. Foods,2022,11(6):855. doi: 10.3390/foods11060855
    [30]
    师希雄, 岳建伟, 张攀高, 等. 宰后H2O2处理的藏羊肉能量代谢、细胞凋亡及嫩化研究[J]. 农业机械学报,2021,52(3):357−363. [SHI X X, YUE J W, ZHANG P G, et al. Tibetan sheep meat energy metabolism, apoptosis and tenderization during postmortem aging after H2O2 treatment[J]. Transactions of the Chinese Society for Agricultural Machinery,2021,52(3):357−363. doi: 10.6041/j.issn.1000-1298.2021.03.040

    SHI X X, YUE J W, ZHANG P G, et al. Tibetan sheep meat energy metabolism、apoptosis and tenderization during postmortem aging after H2O2 treatment[J]. Transactions of the Chinese Society for Agricultural Machinery, 2021, 52(3): 357-363. doi: 10.6041/j.issn.1000-1298.2021.03.040
    [31]
    BHAT Z, MORTON J D, MASON S, et al. Role of calpain system in meat tenderness: A review[J]. Food Science and Human Wellness,2018,7(03):196−204. doi: 10.1016/j.fshw.2018.08.002
    [32]
    张银银. 外源性L-精氨酸和L-赖氨酸对卤鸡胸肉嫩度的影响及机理研究[D]. 合肥: 合肥工业大学, 2020

    ZHANG Y Y. Study on the effect and mechanism of exogenous L-arginine and L-lysine on tenderness of cookedmarinated chicken breast[D] Hefei: University of Technology, 2020.
    [33]
    崔文斌. 不同氧化体系对牦牛肉成熟过程中肌原纤维蛋白生化特性及质构的影响[D]. 兰州: 甘肃农业大学, 2019

    CUI W B. Effects of different oxidation systems on biochemicalcharacteristics and texture of myofibrillar protein during beef ripening[D]. Lanzhou: Gansu Agricultural University, 2019.
    [34]
    SOLADOYE O P, PRIETO N, LOPEZ-CAMPOS O, et al. Potential of near infrared (NIR) spectroscopy and dual energy X-ray absorptiometry (DXA) in predicting pork belly softness[J]. Meat Science,2018,142:1−4. doi: 10.1016/j.meatsci.2018.03.025
    [35]
    SHEN H, ELMORE J S, ZHAO M, et al. Effect of oxidation on the gel properties of porcine myofibrillar proteins and their binding abilities with selected flavour compounds[J]. Food Chemistry,2020,329:127032. doi: 10.1016/j.foodchem.2020.127032
    [36]
    许鹏. 赖氨酸/精氨酸对乳化香肠脂肪和蛋白质氧化及品质影响的研究[D]. 合肥: 合肥工业大学, 2017

    XU P. The study of the effects of lysine/arginine on lipid and protein oxidation and quality properties of emulsion sausages[D]. Hefei: Hefei University of Technology, 2017.
    [37]
    布鑫荣, 王惠惠, 宋艳艳, 等. 羟自由基和高铁肌红蛋白氧化体系对牦牛肉肌原纤维蛋白氨基酸含量变化的影响[J]. 食品科学,2022,43(5):1−10. [BU X R, WANG H H, SONG Y Y, et al. Effect of iron-catalyzed and metmyoglobin oxidizing systems on amino acid changes of yak myofibrillar protein[J]. Food Science,2022,43(5):1−10.

    BU X R, WANG H H, SONG Y Y, et al. Effect of iron-catalyzed and metmyoglobin oxidizing systems on amino acid changes of yak myofibrillar protein[J]. Food Science, 2022, 43(5): 1-10.
    [38]
    张朵朵, 刘永峰, 古明辉, 等. 猕猴桃多酚在羊肉反复冻融过程中的改善肉质和延缓氧化作用[J]. 食品科学,2022,43(16):36−45. [ZHANG D D, LIU Y F, GU M H, et al. Improving quality and delaying oxidation in goat meat under repeated freeze-thaw treatments by polyphenols from thinned young kiwifruit[J]. Food Science,2022,43(16):36−45.

    ZHANG D D, LIU Y F, GU M H, et al. Improving quality and delaying oxidation in goat meat under repeated freeze-thaw treatments by polyphenols from thinned young kiwifruit[J]. Food Science, 2022, 43(16): 36-45.
    [39]
    LÜ Y Q, FENG X C, YANG R, et al. Dual role (promotion and inhibition) of transglutaminase in mediating myo fi brillar protein gelation under malondialdehyde-induced oxidative stress[J]. Food Chemistry,2021,353:129453. doi: 10.1016/j.foodchem.2021.129453
    [40]
    吕远奇. 丙二醛氧化应激对猪肉肌原纤维蛋白乳化和凝胶特性的影响[D]. 杨凌: 西北农林科技大学, 2019

    LV Y Q. Effects of malondialdehyde oxidative stress on emulsifying and gelling properties of pork myofibrillar protein[D]. Yangling: Northwest A&F University, 2019.
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