Optimization of Oligosaccharide Preparation Process and Physiological Activity Analysis of Konjac Crude Powder

ZHANG Wei; SUN Nan; ZHANG Yuyao; WU Xiaoya; ZHAO Yu; ZHANG Bingqian; ZHANG Baoshan

doi:10.13386/j.issn1002-0306.2022100174

Volume 44 Issue 18

Sep. 2023

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Science and Technology of Food Industry > 2023 > 44(18): 147-155. > DOI: 10.13386/j.issn1002-0306.2022100174

ZHANG Wei, SUN Nan, ZHANG Yuyao, et al. Optimization of Oligosaccharide Preparation Process and Physiological Activity Analysis of Konjac Crude Powder [J]. Science and Technology of Food Industry, 2023, 44(18): 147−155. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100174.

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Optimization of Oligosaccharide Preparation Process and Physiological Activity Analysis of Konjac Crude Powder

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China

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Received Date: October 18, 2022
Available Online: July 19, 2023

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Abstract

In this paper, konjac powde were hydrolyzed by Bacillus licheniformis to prepare oligosaccharides. The effects of concentration of konjac powder, inoculum amount of strain, bacteriolysis temperature and hydrolysis time on hydrolysis rate of konjac powder were determined by single factor tests. The antioxidant activity of konjac powder oligosaccharides was evaluated with the scavenging ability of ·OH, ·O₂⁻· and DPPH· as indexes. The prebiotic activity of konjac powder oligosaccharides was investigated by in vitro fermentation test. The results showed that the optimal hydrolysis process parameters was as follows: concentration of konjac powder 20 g/L, inoculation capacity 1×10⁷ CFU/g, hydrolysis temperature 43.6 ℃, hydrolysis time 10.2 h. Under the optimal conditions, the hydrolysis rate of konjac powder was 39.06%±0.12%, and the yield of KGOS was 66.72%. With the prolongation of hydrolysis time, the viscosity of solution decreased from 2430.33 mPa·s to 18.06 mPa·s. When the concentration of konjac powder was 10 mg/mL, the clearance rates of ·OH, ·O₂⁻ and DPPH· were 61.47%, 34.60% and 81.43%, respectively. The OD₆₀₀ of KGOS, glucose and konjac powder on the proliferation of Lactobacillus plantarum, Lactobacillus brevity and Lactobacillus casei in vitro were 0.775, 1.371, 0.236, 0.791, 1.339, 0.224 and 0.916, 1.336, 0.243, respectively. The results provide a theoretical basis for the further processing and utilization of oligosaccharides in konjac powder.
- KGM oligosacchrrides,
- antioxidant activity,
- prebiotics,
- physiological activity,
- thin layer chromatography (TLC)

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References

[1]	杨大伟, 卢智锋. 乙醇脱除魔芋粗粉三甲胺异味的效果研究[J]. 湖北民族学院学报(自然科学版),2014,32(1):1−4. [YANG Dawei, LU Zhifeng. Effect of ethanol on removing trimethlamine from konjac powder[J]. Journal of Hubei Minzu University (Natural Science Edition),2014,32(1):1−4. YANG Dawei, LU Zhifeng. Effect of ethanol on removing trimethlamine from konjac powder[J]. Journal of Hubei Minzu University (Natural Science Edition), 2014, 32(1): 1-4.
[2]	LI Y, DENG R, CHEN N, et al. Review of konjac glucomannan: Isolation, structure, chain conformation and bioactivities[J]. Journal of Single Molecule Research,2013,1(1):7. doi: 10.12966/jsmr.07.03.2013
[3]	NISHINARI K, WILLIAMS P A, PHILLIPS G O. Review of the physico-chemical characteristics and properties of konjac mannan[J]. Food Hydrocolloids,1992,6(2):199−222. doi: 10.1016/S0268-005X(09)80360-3
[4]	董振香, 顾秋亚, 李丹晨, 等. 不同分子质量魔芋甘露聚糖的制备及功效活性分析[J]. 食品与发酵工业,2020,46(8):48−53. [DONG Zhenxiang, GU Qiuya, LI Danchen, et al. Preparation and active component analysis of Konjac mannans with different molecular weights[J]. Food and Fermention Industries,2020,46(8):48−53. DONG Zhenxiang, GU Qiuya, LI Danchen, et al. Preparation and active component analysis of Konjac mannans with different molecular weights[J]. Food and Fermention Industries, 2020, 46(8): 48-53.
[5]	仝泽方, 李利军, 卢美欢, 等. 葡甘聚糖酶制备魔芋葡甘露低聚糖的工艺研究[J]. 中国调味品,2021,46(7):167−170. [TONG Zefang, LI Lijun, LU Meihuan, et al. Study on preparation process of konjac glucomannan oligosaccharide by glucomannase[J]. China Condiment,2021,46(7):167−170. TONG Zefang, LI Lijun, LU Meihuan, et al. Study on preparation process of konjac glucomannan oligosaccharide by glucomannase[J]. China Condiment, 2021, 46(7): 167-170.
[6]	周旻昱. 魔芋葡甘露低聚糖酶法制备及对肉鸡生理活性的研究[D]. 南京: 南京林业大学 ZHOU Wenyu. Study on the enzymatic hydrolysis of glucomannan from konjac and its probiotic activity in broilers[D]. Nanjing: Nanjing Forestry University, 2022.
[7]	YIN Junyi, MA Luyao, XIE Mingyong, et al. Molecular properties and gut health benefits of enzyme-hydrolyzed konjac glucomannans[J]. Carbohydrate Polymers,2020,237:116117.
[8]	张晓. 食药用菌生物降解魔芋葡甘聚糖技术研究[D]. 武汉: 华中科技大学, 2015 ZHANG Xiao. Study on biodegradation of konjac glucomannan by edible and medicinal bacteria[D]. Wuhan: Huazhong University of Science and Technology. 2015
[9]	张盼文, 李浩, 徐钰红, 等. 一株来源于豆豉的地衣芽孢杆菌全基因组学及其风味物质形成分析[J]. 食品与发酵工业,2022,48(19):36−44. [ZHANG Panwen, LI Hao, XU Yuhong, et al. Whole genome and flavor formation analysiss of a Bacillus licheniformis strain from Douchi[J]. Food and Fermentation Industries,2022,48(19):36−44. ZHANG Panwen, LI Hao, XU Yuhong, et al. Whole genome and flavor formation analysiss of a Bacillus licheniformis strain from Douchi[J]. Food and Fermentation Industries, 2022, 48(19): 36-44.
[10]	李娅楠. 乳杆菌与地衣芽孢杆菌复合微贮对稻草营养价值影响及机理研究[D]. 银川: 宁夏大学, 2022 LI Yanan. Effect and mechanism research of compound silage of Lactobacillus and Bacillus licheniformis on nutritional value of rice straw[D]. Yinchuan: Ningxia University, 2022.
[11]	WAN Xin, WEI Lusha, ZHANG Wei, et al. Production, characterization, and prebiotic activity of oligosaccharides from konjac glucomannan by Bacillus amyloliquefaciens WX-1[S]. Journal of Functional Foods, 2022, 88: 104872.
[12]	张百忍, 王显安, 张宝善. 魔芋[M]. 西安: 陕西出版传媒集团, 三秦出版社, 2014: 127−144 ZHANG Bairen, WANG Xian’an, ZHANG Baoshan. Konjac [M]. Xi'an: Shaanxi Publishing Media Group, Sanqin Publishing House, 2014: 127−144
[13]	刘佩瑛. 魔芋学[M]北京: 中国农业出版社, 2004: 285−295 LIU Peiying. Konjac[M] Beijing: China Agricultural Press, 2004: 285−295.
[14]	中华人民共和国国家卫生和计划生育委员会. GB 5009.3-2016食品安全国家标准食品中水分的测定[S]. 北京: 中国标准出版社, 2016: 3 National Health and Family Planning Commission of the People’s Republic of China. GB 5009.3-2016 National food safety standard. Determination of water in food[S]. Beijing: China Standards Press, 2016: 3.
[15]	中华人民共和国农业部. 魔芋粉: NY/T 494-2010[S]. 北京: 中国农业出版社, 2010: 8−9 Ministry of Agriculture of the PRC. Konjac flour: NY/T 494-2010 [S]. Beijing: China Agriculture Press, 2010: 8−9.
[16]	中华人民共和国卫生部中国国家标准化管理委员会. GB/T 5009.10-2003 植物类食品中粗纤维的测定[S]. 北京: 中国标准出版社, 2003: 3 The Ministry of Health of the People’s Republic of China China National Standardization Administration. GB/T 5009.10-2003 Determination of crude fiber in plant food[S]. Beijing: China Standards Press, 2003: 3.
[17]	中华人民共和国国家卫生和计划生育委员会国家食品药品监督管理总局. GB 5009.5-2016 食品安全国家标准食品中蛋白质的测定[S]. 北京: 中国标准质检出版社, 2016: 3−5 State Food and Drug Administration of the State Health and Family Planning Commission of the People’s Republic of China. GB 5009.5-2016 National food safety standard. Determination of protein in food[S]. Beijing: China Standards and Quality Inspection Press, 2016: 3−5.
[18]	国家食品药品监督管理总局国家卫生和计划生育委员会. GB 5009.6-2016 食品安全国家标准食品中脂肪的测定[S]. 北京: 中国标准出版社, 2016: 3−4 State Health and Family Planning Commission of the State Food and Drug Administration. GB 5009.6-2016 National food safety standard. Determination of fat in food[S] Beijing: China Standards Press, 2016: 3−4.
[19]	国家卫生和计划生育委员会. GB 5009.4-2016 食品安全国家标准食品中灰分的测定[S]. 北京: 中国标准出版社, 2016: 3−6 National Health and Family Planning Commission. GB 5009.4-2016 National food safety. Determination of ash in food[S] Beijing: China Standards Press, 2016: 3−6.
[20]	国家卫生和计划生育委员会. GB 5009.237-2016 食品安全国家标准食品pH值的测定[S]. 北京: 中国标准出版社, 2016: 3−6 National Health and Family Planning Commission. GB 5009.237-2016 National food safety standard. Determination of food pH[S]. Beijing: Standards Press of China, 2016: 3−6.
[21]	CHEN J, LIU D, SHI B, et al. Optimization of hydrolysis conditions for the production of glucomanno-oligosaccharides from konjac using β-mannanase by response surface methodology[J]. Carbohydrate Polymers,2013,93(1):81−88. doi: 10.1016/j.carbpol.2012.05.037
[22]	ZHANG Yuan, ZHAO Yi, YANG Wanfu, et al. Structural complexity of konjac glucomannan and its derivatives governs the diversity and outputs of gut microbiota[J]. Carbohydrate Polymers,2022,292:119639. doi: 10.1016/j.carbpol.2022.119639
[23]	李文超. 特定聚合度魔芋低聚糖的筛选与制备技术研究[D]. 武汉: 华中科技大学, 2014 LI Wenchao. Screening and preparation technology analysis of konjac oligosaccharides with specific polymerization degree[D]. Wuhan: Huazhong University of Science and Technology, 2014.
[24]	郭丽萍. 不同水化与流变学性质魔芋葡甘露聚糖调控血糖和抑制肥胖的功能研究[D]. 无锡: 江南大学, 2021 GUO Liping. Study on the function of konjac glucomannan with different hydration and rheological properties in regulating glycemic response and preventing obesity[D]. Wuxi: Nanjing University. 2021
[25]	张敏. 微波-酶法从野皂荚胶制备半乳甘露低聚糖技术研究[D]. 南京: 南京农业大学, 2008 ZHANG Min. Study on preparation of galacto-mannan-oligosaccharides by microwave-enzyme hydrolysis of g. heterophylla gum in technology[D]. Nanjing: Nanjing Agricultural University, 2008.
[26]	LI W A, PZZ A, JWS B, et al. Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular weights extracted from Pleurotus ostreatus[J]. International Journal of Biological Macromolecules,2019,141:150−160. doi: 10.1016/j.ijbiomac.2019.08.250
[27]	CHENG H, HUANG G. Extraction, characterisation and antioxidant activity of Allium sativum polysaccharide[J]. International Journal of Biological Macromolecules,2018,114:415−419. doi: 10.1016/j.ijbiomac.2018.03.156
[28]	WU Caiyun, LI Tianlin, QI Jing, et al. Effects of lactic acid fermentation-based biotransformation on phenolic profiles, antioxidant capacity and flavor volatiles of apple juice[J]. LWT, 2020, 122: 109064.
[29]	李斌, 谢笔钧. 魔芋葡甘聚糖凝胶机理研究[J]. 中国农业科学,2002,35(11):1411−1415. [LI Bin, XIE Bijun. Study on the gelatin mechanism of konjac glucomannans[J]. Scientia Agricultura Sinica,2002,35(11):1411−1415. LI Bin, XIE Bijun. Study on the gelatin mechanism of konjac glucomannans[J]. Scientia Agricultura Sinica, 2002, 35(11): 1411-1415.
[30]	YIN J Y, MA L Y, XIE M Y, et al. Molecular properties and gut health benefits of enzyme-hydrolyzed konjac glucomannans[J]. Carbohydrate Polymers,2020,237:116117. doi: 10.1016/j.carbpol.2020.116117
[31]	JIAN W J, SUNY M, HUANG H, et al. Study on preparation and separation of konjac oligosaccharides[J]. Carbohydrate Polymers,2013,92(2):1218−1224. doi: 10.1016/j.carbpol.2012.09.065
[32]	郑梅霞, 朱育菁, 刘波, 等. 黄原胶的流变性及与魔芋胶等的协效性研究[J]. 食品工业科技,2016,37(8):303−306,322. [ZHENG Meixia, ZHU Yujing, LIU Bo, et al. Study on the rheology of xanthan and synergistic interaction with konjac gum and other gump[J]. Science and Technology of Food Industry,2016,37(8):303−306,322. ZHENG Meixia, ZHU Yujing, LIU Bo, et al. Study on the rheology of xanthan and synergistic interaction with konjac gum and other gump[J]. Science and Technology of Food Industry, 2016, 37(8): 303-306, 322.
[33]	JANA U K, KANGO N. Characteristics and bioactive properties of mannooligosaccharides derived from agro-waste mannans[J]. International Journal of Biological Macromolecules,2020,149:931−940. doi: 10.1016/j.ijbiomac.2020.01.304
[34]	QU W J, FENG Y T, XIONG T, et al. Preparation of corn ACE inhibitory peptide-ferrous chelate by dual-frequency ultrasound and its structure and stability analyses[J]. Ultrasonics Sonochemistry,2022,83:105937. doi: 10.1016/j.ultsonch.2022.105937
[35]	常相娜, 陈雪峰, 苏瑶. 酶解苹果渣中多糖的结构分析及其体外抗氧化活性[J]. 陕西科技大学学报,2022,40(5):63−69. [CHANG Xiangna, CHEN Xuefeng, SU Yao, et al. Structure analysis and in vitro antioxidant activity of polysaccharides fro enzymatic hydrolysis apple pomace[J]. Journal of Shaanxi University of Science & Technology,2022,40(5):63−69. CHANG Xiangna, CHEN Xuefeng, SU Yao, et al. Structure analysis and in vitro antioxidant activity of polysaccharides fro enzymatic hydrolysis apple pomace[J]. Journal of Shaanxi University of Science & Technology, 2022, 40(5): 63-69.
[36]	王艳妮. 魔芋全粉酶解方法及其特性研究[D]. 长沙: 湖南农业大学, 2019 WANG Yanni. Study on properties and enzymatic hydrolysis method of konjac powder[D]. Changsha: Hunan Agricultural University, 2019.
[37]	葛珍珍, 许明月, 靳学远, 等. 脱乙酰基魔芋葡甘聚糖理化性质及其对凉皮质构和体外消化的影响[J]. 食品科学,2023,44(4):85−90. [GE Zhenzhen, XU Mingyue, JIN Xueyuan, et al. The physicochemical properties of deacetyiated konjac giucomannan and its effects on the quality andin vitro digestion of liangpi[J]. Food Science,2023,44(4):85−90. GE Zhenzhen, XU Mingyue, JIN Xueyuan, et al. The physicochemical properties of deacetyiated konjac giucomannan and its effects on the quality and in vitro digestion of liangpi[J]. Food Science, 2023, 44(4): 85−90.
[38]	孟凡冰, 王小燕, 范定涛, 等. 脱敏魔芋粉制备集成技术初探及其对魔芋粉理化性质的影响[J]. 食品科学,2015,36(2):63−68. [MENG Fanbing, WANG Xiaoyan, FAN Dingtao et al. Preparation of konjac flour by combined use of different techniques and its physicochemical properties[J]. Food Science,2015,36(2):63−68. MENG Fanbing, WANG Xiaoyan, FAN Dingtao et al. Preparation of konjac flour by combined use of different techniques and its physicochemical properties[J]. Food Science, 2015, 36(2): 63-68.

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