Study on the Bacteriostatic Activity and Stability of Ethanolic Extract from Yunnan <i>Litsea cubeba</i>

CHEN Cong; XUE Qiaoli; HU Yongjin; WEI Meijuan; CHEN Zhongai

doi:10.13386/j.issn1002-0306.2022100111

Volume 44 Issue 16

Aug. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(16): 147-154. > DOI: 10.13386/j.issn1002-0306.2022100111

CHEN Cong, XUE Qiaoli, HU Yongjin, et al. Study on the Bacteriostatic Activity and Stability of Ethanolic Extract from Yunnan Litsea cubeba[J]. Science and Technology of Food Industry, 2023, 44(16): 147−154. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100111.

Citation:

PDF (4182 KB)

Study on the Bacteriostatic Activity and Stability of Ethanolic Extract from Yunnan Litsea cubeba

1.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China
2.
Editorial Office of Journal of Yunnan Agricultural University, Kunming 650201, China

More Information

Received Date: October 13, 2022
Available Online: June 10, 2023

Graphical Abstract

Abstract

Abstract

In this study, the antibacterial effects of five different ethanolic concentrations (0%, 55%, 75%, 95%, 100%) EELC (Ethanol extract of Yunnan Litsea cubeba) on two kinds of pathogenic bacteria were determined. Ethanol extracts which had the strongest antibacterial activity and their stability were analyzed. The inhibition zone diameter of different concentrations in ethanolic EELC against E.coil and S.aureus were determined by KB paper method. At the same time, using TTC (2,3,5-triphenyltetrazolium chloride) method detected the MIC of EELC to screen out the best antibacterial activity of EELC. At the end, the temperature, heat treatment time, pH and UV light stability of EELC were further studied. The results showed that 55% and 75% EELC had the best inhibitory effect on S.aureus and E.coli, with MIC of 19 and 819 μg/mL, respectively. 55% EELC treated at 40 ℃, the OD₆₀₀ was 0.118, which had the optimal antibacterial activity. Furthermore, the antibacterial activity remained stable after treatment for 1~5 hours at this condition. There was no significant difference in antibacterial activity between the two groups after irradiation with 30W UV light for 10~60 min (P>0.05). The bacteriostatic activity decreased at pH4, while it remained at a stable level at pH6~10. 75% EELC was treated at 100 ℃, the retention rate of antibacterial activity reaching 92.88%, and no significant difference was found in antibacterial activity after heating at this temperature for 1~5 h (P>0.05). The antibacterial activity was significantly decreased at pH4~8 (P<0.05), but it showed increasing trend at pH10. UV light irradiation for 10~60 min had no significant effect on the inhibitory activity (P>0.05). It was suggested tha, the 55% EELC had a high stability and low action concentration against S.aureus, while 75% EELC had a stable inhibition effect against E.coil. The results would provide a theoretical basis for the comprehensive utilization and development of Yunnan Litsea cubeba.
- Yunnan Litsea cubeba,
- ethanolic extract,
- antibacterial activity,
- minimal inhibitory concentration,
- stability

FullText(HTML)

References (33)

References

[1]	李萍, 钱波, 王霞. 食品安全现状及食品安全检测[J]. 食品安全导刊,2022(22):10−12. [LI P, QIAN B, WANG X. Current Situation of Food Safety and Food Safety Inspection[J]. China Food Safety Magazine,2022(22):10−12. doi: 10.3969/j.issn.1674-0270.2022.22.spaqdk202222012 LI P, QIAN B, WANG X. Current Situation of Food Safety and Food Safety Inspection[J]. China Food Safety Magazine, 2022(22): 10-12. doi: 10.3969/j.issn.1674-0270.2022.22.spaqdk202222012
[2]	贾睿蔡丹, 葛思彤, 等. 红豆皮多酚提取物对两种致病菌的抑菌活性及作用机理[J]. 食品科学,2021,42(23):64−71. [JIA R, CAI D, GE S T, et al. Antibacterial activity and mechanism of polyphenol extracts from Adzuki bean seed coat against two pathogens[J]. Food Science,2021,42(23):64−71. JIA R, CAI D, GE S T, et al. Antibacterial activity and mechanism of polyphenol extracts from Adzuki bean seed coat against two pathogens [J] Food Science, 2021, 42 (23): 64-71
[3]	龙婧, 李婷婷, 王全林, 等. 快速检测金黄色葡萄球菌的一种氮源的酶解优化策略[J]. 微生物学杂志,2018,38(3):29−35. [LONG J, LI T T, WANG Q L, et al. Optimization tactics of enzymatic hydrolysis of nitrogen source for rapid detection of Staphylococcus aureus[J]. Journal of Microbiology,2018,38(3):29−35. LONG J, LI T T, WANG Q L, et al. Optimization tactics of enzymatic hydrolysis of nitrogen source for rapid detection of Staphylococcus aureus [J]. Journal of Microbiology, 2018, 38 (3): 29−35.
[4]	ALVAREZ-FERNANDEZ E, CANCELO A, DIAZ-VEGA C, et al. Antimicrobial resistance in E. coli isolates from conventionally and organically reared poultry: A comparison of agar disc diffusion and SensiTest Gram-negative methods[J]. Food Control,2013,30(1):227−234. doi: 10.1016/j.foodcont.2012.06.005
[5]	KAPER J B. Pathogenic Escherichia coli[J]. International Journal of Medical Microbiology,2005,295(6/7):355.
[6]	CUI H Y, ZHANG C H, LI C Z, et al. Inhibition of Escherichia coli O157:H7 biofilm on vegetable surface by solid liposomes of clove oil[J]. LWT-Food Science and Technology,2020,117:108656. doi: 10.1016/j.lwt.2019.108656
[7]	VáSQUEZ-GARCíA A, DE OLIVEIRA A P S C D, MEJIABALLESTEROS J E, et al. Escherichia coli detection and identification in shellfish from southeastern Brazil[J]. Aquaculture,2019,504:158−163. doi: 10.1016/j.aquaculture.2019.01.062
[8]	李文菁. 核桃青皮提取物抑菌活性及抑菌机理研究[D]. 乌鲁木齐: 新疆农业大学, 2021 LI W J. Study on antibacterial activity and mechanism of walnut peel green husk extract [D] Urumqi: Xinjiang Agricultural University, 2021.
[9]	张伟豪, 翁道明, 宋慧云, 等. 9种夹竹桃科和大戟科植物抗菌和抗氧化活性测定[J]. 南方农业学报,2018,49(1):85−90. [ZHANG W H, WENG D M, SONG H Y, et al. Identification on antibacterial activity and antioxidant activity of nine Apocynaceae and Euphorbiaceae plants[J]. Journal of Southern Agriculture,2018,49(1):85−90. doi: 10.3969/j.issn.2095-1191.2018.01.14 ZHANG W H, WENG D M, SONG H Y, et al. Identification on antibacterial activity and antioxidant activity of nine Apocynaceae and Euphorbiaceae plants [J]. Journal of Southern Agriculture, 2018, 49(1): 85-90. doi: 10.3969/j.issn.2095-1191.2018.01.14
[10]	HU W, LI C Z, DAI J M, et al. Antibacterial activity and mechanism of Litsea cubeba essential oil against methicillin-resistant Staphylococcus aureus (MRSA)[J]. Industrial Crops and Products, 2019: 34−41.
[11]	张丽, 陈尚钘, 范国荣, 等. 山苍子精油的化学成分和功能活性及其应用研究进展[J]. 江西农业大学学报,2021,43(2):355−363. [ZHANG L, CHEN S X, FAN G R, et al. Research progress in application and functions of Litsea cubeba essential oil components[J]. Acta Agriculturae Universitatis Jiangxiensis,2021,43(2):355−363. ZHANG L, CHEN S X, FAN G R, et al. Research progress in application and functions of Litsea cubeba essential oil components[J]. Acta agriculturae universitatis Jiangxiensis, 2021, 43(2): 355-363. ]
[12]	黄志端. 木姜子的研究进展及应用价值[J]. 当代化工研究,2022(9):164−166. [HUANG Z D. Research progress and application value of Zingiberaceae[J]. Modern Chemical Research,2022(9):164−166. doi: 10.3969/j.issn.1672-8114.2022.09.053 HUANG Z D. Research progress and application value of Zingiberaceae [J]. Modern Chemical Research, 2022(9): 164-166. doi: 10.3969/j.issn.1672-8114.2022.09.053
[13]	李捷, 李锡文. 中国樟科木姜子属植物纪要[J]. 云南植物研究,2006,28(2):103−107. [LI J, LI X W. A summary of the genus Lauraceae in China[J]. Yunnan Botanical Research,2006,28(2):103−107. LI J, LI X W. A summary of the genus Lauraceae in China[J]. Yunnan Botanical Research, 2006, 28(2): 103-107.
[14]	谢宗万, 余友芩. 全国中草药名鉴[M]. 北京人民卫生出版社, 1996: 5 XIE Z W, YU Y Q. National Chinese herbal medicine list [M]. Beijing People's Medical Publishing House, 1996: 5.
[15]	KONG D G, ZHAO Y, LI G H, et al. The genus Litsea in traditional Chinese medicine: An ethnomedical, phytochemical and pharmacological review[J]. Journal of Ethnopharmacology,2015,164:256−264. doi: 10.1016/j.jep.2015.02.020
[16]	中国油脂植物编写委员会. 中国油脂植物[M]. 北京: 北京科学出版社, 1987: 143−144 China Oil Plant Compilation Committee. Chinese oil plant [M]. Beijing: Beijing Science Press, 1987: 143−144.
[17]	曾朝懿, 张琦, 何绍志, 等. 响应曲面法结合模糊感官评定在木姜子风味酸辣酱研制中的应用[J]. 中国调味品,2022,47(7):77−83. [ZENG C Y, ZHANG Q, HE S Z, et al. Application of response surface methodology combined with fuzzy sensory evaluation in the development of Litsea cubeba flavor chutney[J]. China Condiment,2022,47(7):77−83. ZENG C Y, ZHANG Q, HE S Z, et al. Application of response surface methodology combined with fuzzy sensory evaluation in the development of Litsea cubeba flavor chutney [J]. China Condiment, 2022, 47(7): 77-83.
[18]	TSAI I L, CHENG M J, HUNG H W, et al. Chemical constitunets from the leaves of Litsea acutivena[J]. Journal of the Chinese Chemical Society,2007,54(2):503−506. doi: 10.1002/jccs.200700071
[19]	杨秀芳, 王媛, 马养民, 等. 木姜子枝叶中化学成分活性的研究[J]. 陕西科技大学学报(自然科学版),2014,32(6):78−81. [YANG X F, WANG Y, MA Y M, et al. Anti-microbial activity of compounds from Litsea pungens[J]. Journal of Shanxi University of Science & Technoology (Natural Science Edition),2014,32(6):78−81. YANG X F, WANG Y, MA Y M, et al. Anti-microbial activity of compounds from Litsea pungens [J]. Journal of Shanxi University of Science & Technoology (Natural Science Edition), 2014, 32(6): 78-81.
[20]	DAI J, LI C, CUI H, et al. Unraveling the anti-bacterial mechanism of Litsea cubeba essential oil against E. coli O157: H7 and its application in vegetable juices[J]. International Journal of Food Microbiology,2021,338:108989. doi: 10.1016/j.ijfoodmicro.2020.108989
[21]	孙畅, 李湘, 王银红, 等. 山苍子精油对柑橘意大利青霉的抑菌活性及作用机制[J/OL]. 食品科学: 1−15[2022-12-05]. http://kns.cnki.net/kcms/detail/11.2206.TS.20221009.1552.002.html SUN C, LI X, WANG Y H, et al. Antibacterial activity and mechanism of Litsea cubeba essential oil against Penicillium italicum in citrus fruit [J/OL]. Food Science: 1−15[2022-10-12]. http://kns.cnki.net/kcms/detail/11.2206.TS.20221009.1552.002.html
[22]	李欣越, 苟玉虹, 申瀚君, 等. 山苍子精油抑制沙门菌作用机制研究[J]. 四川农业大学学报,2021,39(3):385−390. [LI X Y, GOU Y H, SHEN H J, et al. Antibacterial mechanism of Litsea cubeba essential oil on Salmonella[J]. Journal of Sichuan Agricultural University,2021,39(3):385−390. LI X Y, GOU Y H, SHEN H J, et al. Antibacterial Mechanism of Litsea cubeba Essential Oil on Salmonella [J]. Journal of Sichuan Agricultural University, 2021, 39(3): 385-390.
[23]	聂冬阳, 郝凯源, 徐百昌, 等. 山苍子精油与丁香精油对金黄色葡萄球菌的协同抑菌作用[J]. 饲料研究,2022(16):78−82. [NIE D Y, HAO K Y, XU B C, et al. Synergistic bacteriostatic effect of Litsea cubeba essential oil and clove essential oil on Staphylococcus aureus[J]. Feed Reseaech,2022(16):78−82. NIE D Y, HAO K Y, XU B C, et al. Synergistic bacteriostatic effect of Litsea cubeba essential oil and clove essential oil on Staphylococcus aureus[J]. Feed Reseaech, 2022（16）: 78−82.
[24]	赵红艳, 张淑颖, 张玲, 等. 木姜子乙醇提取物抑菌活性研究[J]. 贵州大学学报(自然科学版),2008(5):543−545. [ZHAO H Y, ZHANG S Y, ZHANG L, et al. Study on fungicide activity of Nelitsea sericea Koidz extracts to several kind sof plant fungi[J]. Journal of Guizhou University (Natural Sciences),2008(5):543−545. doi: 10.3969/j.issn.1000-5269.2008.05.025 [ZHAO H Y, ZHANG S Y, ZHANG L, et al. Study on fungicide activity of Nelitsea sericea Koidz extracts to several kind sof plant fungi[J]. Journal of Guizhou University (Natural Sciences), 2008(5): 543-545. doi: 10.3969/j.issn.1000-5269.2008.05.025
[25]	罗耀玲, 黄铀新, 杨建琼, 等. 木姜子乙醇提取物对肝癌细胞侵袭和迁移能力的影响及机制初探[J]. 中国医院药学杂志,2017,37(9):839−842. [LUO Y L, HUANG Y X, YANG J Q, et al. Effects and mechanism of ethanol extract of Litsea pungens on migration and invasion of hepatocellular carcinoma cells[J]. Chinese Journal of Hospital Pharmacy,2017,37(9):839−842. LUO Y L, HUANG Y X, YANG J Q, et al. Effects and mechanism of ethanol extract of Litsea pungens on migration and invasion of hepatocellular carcinoma cells [J]. Chinese Journal of Hospital Pharmacy, 2017, 37（9）: 839-842.
[26]	中国标准化研究院. GB/T 38483-2020微生物源抗生素类次生代谢产物抗细菌活性测定: 抑菌圈法[S]. 2020-11-19 China institute of standardization. GB/T 38483-2020 Determination of antibacterial activity of secondary metabolites of antibiotics of microbial origin: Inhibition zone method[S]. 2020-11-19.
[27]	SAVIANO A M, LOURENCO F R. Rapid microbiological methods (RMMs) for evaluating the activity of cephalosporin antibiotics employing triphenyltetrazolium chloride[J]. Talanta,2018,185:520−527. doi: 10.1016/j.talanta.2018.04.020
[28]	宋姗姗, 杨艾华, 王小敏等. 湄潭白茶多糖对金黄色葡萄球菌的抑菌活性及其稳定性[J]. 食品工业科技,2022,43(3):114−119. [SONG S S, YANG A H, WANG X M, et al. Antibacterial activity and stability of polysaccharides from meitan white tea on Staphylococcus aureus[J]. Science and Technology of Food Industry,2022,43(3):114−119. SONG S S, YANG A H, WANG X M, et al. Antibacterial activity and stability of polysaccharides from meitan white tea on Staphylococcus aureus [J]. Science and Technology of Food Industry, 2022, 43(3): 114-119.
[29]	艾薇, 张星和, 冯李院. 山姜精油抑菌活性及稳定性研究[J]. 中国食品添加剂,2020,31(9):8−13. [AI W, ZHANG X H, FENG L Y. Study on antimicrobial activity and stability of essential oil from Alpinia japonica[J]. China Food Additives,2020,31(9):8−13. AI W, ZHANG X H, FENG L Y. Study on antimicrobial activity and stability of essential oil from Alpinia japonica [J]. China Food Additives, 2020, 31(9): 8-13.
[30]	彭湘莲, 付红军, 刘微微. 湘西永顺产山苍子精油成分分析与抑菌活性研究[J]. 中国粮油学报,2018,33(11):61−64,78. [PENG X L, FU H J, LIU W W. Antibacterial activity research and composition analysis on Litsea cubeba essential oil produced in Yongshun of western Hunan Province[J]. Journal of the Chinese Cereals and Oils Association,2018,33(11):61−64,78. doi: 10.3969/j.issn.1003-0174.2018.11.011 PENG X L, FU H J, LIU W W. Antibacterial activity research and composition analysis on Litsea cubeba essential oil produced in Yongshun of western Hunan Province[J]. Journal of the Chinese Cereals and Oils Association, 2018, 33(11): 61-64, 78. doi: 10.3969/j.issn.1003-0174.2018.11.011
[31]	肖勇, 李良. 木姜子属挥发油成分及其生物活性研究概况[J]. 云南化工,2007,34(5):85−92. [XIAO Y, LI L. Research advance on essential oils from Litsea and their biological activity[J]. Yunnan Chemical Technology,2007,34(5):85−92. doi: 10.3969/j.issn.1004-275X.2007.05.022 XIAO Y, LI L. Research advance on essential oils from Litsea and their biological activity[J]. Yunnan Chemical Technology, 2007, 34（5）: 85-92. doi: 10.3969/j.issn.1004-275X.2007.05.022
[32]	韦春玲, 崔培梧, 肖瑾, 等. 具有抗菌活性的天然二萜类化合物研究进展[J]. 中成药,2022,44(7):2240−2249. [WEI C L, CUI P W, XIAO J, et al. Research progress of natural diterpenoids with antibacterial activity[J]. Chinese Traditional Patent Medicine,2022,44(7):2240−2249. doi: 10.3969/j.issn.1001-1528.2022.07.030 WEI C L, CUI P W, XIAO J, et al. Research progress of natural diterpenoids with antibacterial activity[J]. Chinese Traditional Patent Medicine, 2022, 44（7）: 2240-2249. doi: 10.3969/j.issn.1001-1528.2022.07.030
[33]	LIU T T, YANG T S. Antimicrobial impact of the components of essential oil of Litsea cubeba from Taiwan and antimicrobial activity of the oil in food systems[J]. International Journal of Food Microbiology,2016,156(1):68−75.

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	唐佳代，石雨菲，赵益梅，王怡，冷枝，郭敏，孟卓妮，杨亮. 不同感官特性酱香型大曲理化指标与霉菌群落关联分析. 食品工业科技. 2024(03): 153-161 . 本站查看
2.	王洋，谢菲，杜礼泉，范昌明，冯波. 酿酒专用小麦大曲中挥发性风味成分与微生物群落相关性分析. 中国酿造. 2024(02): 71-81 .
3.	韩冰玙，陈辉太，李宗军. 酱、浓、清香型白酒酿造过程中真菌及其功能特性研究进展. 中国酿造. 2024(03): 27-32 .
4.	宋文霞，徐媛媛，韩小龙，李桂菊，成冬冬，林范学，秦宏伟. 人工窖泥在浓香型白酒酿造中微生物群落结构的变化. 食品科技. 2024(06): 40-46 .