Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

GU Yue; TANG Huixin; LI Shuo; MA Ling; WANG Ke; YANG Lili

doi:10.13386/j.issn1002-0306.2022100072

Volume 44 Issue 18

Sep. 2023

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Science and Technology of Food Industry > 2023 > 44(18): 313-320. > DOI: 10.13386/j.issn1002-0306.2022100072

GU Yue, TANG Huixin, LI Shuo, et al. Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(18): 313−320. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100072.

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Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

GU Yue^1,,
TANG Huixin¹,
LI Shuo²,
MA Ling²,
WANG Ke^{1, 2, ,},
YANG Lili¹

1.
College of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang 050024, China
2.
Shijiazhuang Technology Innovation Center for Chemical Poison Detection and Risk Early Warning, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011, China

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Received Date: October 09, 2022
Available Online: July 19, 2023

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Abstract

Abstract

A method for the determination of 10 tetracycline antibiotics in fruit products and meat sauce was established by QuEChERS coupled with ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS). The optimal experimental conditions were determined by optimizing the mass spectrometric parameters, chromatographic conditions and pre-treatment techniques. The samples were extracted by 0.2% formic acid acetonitrile under ultrasonication, and then purified with PSA for fruit products and C₁₈ for meat sauce. The target analytes were separated on a ZORBAX SB-Aq column (2.1 mm×150 mm, 3.5 μm), and detected by UPLC-MS/MS. The results demonstrated that the 10 tetracycline antibiotics showed good linearity in the respective linear range with the correlation coefficients (r) higher than 0.999. The limits of detection (S/N=3) were 0.09~3.92 μg/kg, and the limits of quantification (S/N=10) were 0.31~13.05 μg/kg. The average recoveries at three different spiked levels of 75, 300 and 750 μg/kg ranged from 70.0% to 101.0% with the relative standard deviation (RSD) of 1.0%~10.3% (n=7). The method is sensitive, simple and suitable for the rapid and simultaneous detection of 10 tetracycline antibiotics in fruit products and meat sauce.

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