Anti-photoaging Effect and Mechanism of Tomato and <i>Aronia melanocarpa</i> Extract

LUO Zhen; NING Chuguang; LI Zuozhen; YAO Chunyan; WEI Xiaoli; LI Hongying; CAI Ya; JIANG Shandong

doi:10.13386/j.issn1002-0306.2022090229

Volume 44 Issue 16

Aug. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(16): 395-402. > DOI: 10.13386/j.issn1002-0306.2022090229

LUO Zhen, NING Chuguang, LI Zuozhen, et al. Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract[J]. Science and Technology of Food Industry, 2023, 44(16): 395−402. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090229.

Citation:

PDF (2661 KB)

Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract

1.
Infinitus (China) Company Ltd., Guangzhou 510623, China
2.
Hangzhou Medical College, Hangzhou 310013, China
3.
Healthy-Star Biological Technology Research and Development (Shanghai) Co., Ltd., Shanghai 200335, China)

More Information

Received Date: September 21, 2022
Available Online: June 10, 2023

Graphical Abstract

Abstract

Abstract

In order to investigate the anti-photoaging effect and mechanism of tomato and aronia extracts, the effects of tomato extract and aronia extract on apoptosis, reactive oxygen species (ROS) and prostaglandin E₂ (PGE₂) were studied in UVB-irradiated HaCaT cells. Hematoxylin-eosin (HE) staining was used to evaluate the anti-photoaging effect of tomato and aronia complex (LA) on skin photoaging in UVB-irradiated ICR mice. The activity of superoxide dismutase (SOD), the level of malondialdehyde (MDA) and the expression of pro-inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) were detected. The results showed that tomato extract and aronia extract were not cytotoxic to HaCaT cells. In the photoaging model of HaCaT cells, compared with the model group, the number of apoptotic cells in 100 μg/mL tomato extract group and 30μg/mL aronia extract group were significantly decreased (P<0.05). Aronia extract (100 μg/mL) significantly decreased ROS level (P<0.01). At 100 μg/mL, PEG₂ was significantly decreased in both tomato and aronia extract groups (P<0.05). Compared with the model group, LA treatment group resulted in a significant reduction in dermal thickness (P<0.01) and MDA levels (P<0.01), and a significant increase in SOD activity (P<0.01) in skin tissue, while the proinflammatory factors IL-6, COX-2 and TNF-α were all significantly decreased (P<0.05). Tomato and aronia complex can improve oxidative stress and inflammation induced by UVB, and has the effect of anti-photoaging.
- tomato,
- Aronia melanocarpa,
- skin,
- UVB,
- photoaging

FullText(HTML)

References (41)

References

[1]	ZHANG S, DUAN E. Fighting against skin aging: the way from bench to bedside[J]. Cell Transplantation, 2018, 27(2): 096368971772575.
[2]	CAVINATO M, JANSEN D P. Molecular mechanisms of UVB-induced senescence of dermal fibroblasts and its relevance for photoaging of the human skin[J]. Experimental Gerontology,2017,94:78−82. doi: 10.1016/j.exger.2017.01.009
[3]	GU Y, HAN J, JIANG C, et al. Biomarkers, oxidative stress and autophagy in skin aging[J]. Ageing Research Reviews,2020,59:101036. doi: 10.1016/j.arr.2020.101036
[4]	杨斌, 刁庆春, 钟白玉, 等. 番茄红素对长波紫外线照射大鼠皮肤中丙二醛形成和基质金属蛋白酶表达的抑制作用[J]. 中华医学美学美容杂志,2006,12(6):358−361. [YANG B, DIAO Q C, ZHONG B Y, et al. Lycopene prevents UVA-induced MDA formation and matrix metalloproteinases mRNA expression in rat skin[J]. Chinese Journal of Medical Aesthetics and Cosmetology,2006,12(6):358−361. doi: 10.3760/cma.j.issn.1671-0290.2006.06.011 YANG B, DIAO Q C, ZHONG B Y, et al. Lycopene prevents UVA-induced MDA formation and matrix metalloproteinases mRNA expression in rat skin[J]. Chin J Med Aesth, 2006, 12(6): 358-361. doi: 10.3760/cma.j.issn.1671-0290.2006.06.011
[5]	SANTOS V M, CAMILO A G, SOUZA L A, et al. A woman with treated breast cancer, recent neurological symptoms and xanthoderma[J]. Acta Medica Iranica, 2013: 195−198.
[6]	MELÉNDEZ M A J, MAPELLI B P, STINCO C M. The colourless carotenoids phytoene and phytofluene: From dietary sources to their usefulness for the functional foods and nutricosmetics industries[J]. Journal of Food Composition and Analysis,2018,67:91−103. doi: 10.1016/j.jfca.2018.01.002
[7]	MELÉNDEZ M A J, STINCO C M, MAPELLI B P. Skin carotenoids in public health and nutricosmetics: The emerging roles and applications of the UV radiation-absorbing colourless carotenoids phytoene and phytofluene[J]. Nutrients,2019,11(5):1093. doi: 10.3390/nu11051093
[8]	VON O B L, HAVAS F, RAMOT O, et al. Phytoene and phytofluene for (photo) protection, anti-aging, lightening and evening of skin tone[J]. Sofw Journal Internationales Journal Fur Angewandte Wissenschaft,2014,140:8−12.
[9]	VON O B L, FISHBEIN D, HAVAS F, et al. The photoprotective effects of a food supplement tomato powder rich in phytoene and phytofluene, the colorless carotenoids, a preliminary study[J]. Glob Dermatol,2015,2:178−182.
[10]	史俊燕, 邓天, 侯相林. 野樱莓果实提取物抗氧化性研究[J]. 农产品加工,2015,8(15):14−16. [SHI J Y, DENG T, HOU X L. Antioxidative activity from extracts of aronia[J]. Farm Products Processing,2015,8(15):14−16. SHI J Y, DENG T S, HOU X L. Antioxidative activity from extracts of aronia[J]. Farm Products Processing, 2015, 8: 14-16.
[11]	GOH A R, YOUN G S, YOO K Y, et al. Aronia melanocarpa concentrate ameliorates pro-inflammatory responses in HaCaT keratinocytes and 12-O-tetradecanoylphorbol-13-acetate-induced ear edema in mice[J]. Journal of Medicinal Food,2016,19(7):654−662. doi: 10.1089/jmf.2015.3624
[12]	HER Y, LEE T K, KIM J D, et al. Topical application of aronia melanocarpa extract rich in chlorogenic acid and rutin reduces UVB-induced skin damage via attenuating collagen disruption in mice[J]. Molecules,2020,25(19):4577. doi: 10.3390/molecules25194577
[13]	ZHAO Y, LIU X, ZHENG Y, et al. Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota[J]. Scientific Reports,2021,11(1):1−15. doi: 10.1038/s41598-020-79139-8
[14]	FURUMURA M, SATO N, KUSABA N, et al. Oral administration of french maritime pine bark extract (Flavangenol(®)) improves clinical symptoms in photoaged facial skin. Clinical Interventions in Aging. 2012, 7: 275-86.
[15]	戴妍, 林捷, 郑华, 等. 鸡肉酶解物对HepG2细胞抗氧化活性及小鼠血清抗氧化酶活性的影响[J]. 食品工业科技,2020,41(15):310−314,331. [DAI Y, LIN J, ZHENG H, et al. Effect of chicken hydrolysate on antioxidative activity of HepG2 cells and antioxidative enzyme activity of mouse serum[J]. Science and Technology of Food Industry,2020,41(15):310−314,331. DAI Y, LIN J, ZHENG H, et al. Effect of chicken hydrolysate on antioxidative activity of HepG2 cells and antioxidative enzyme activity of mouse serum[J]. Science and Technology of Food Industry, 2020, 41(15): 310- 314, 331.
[16]	MUZAFFER U, PAUL V I, PRASAD N R, et al. Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes[J]. Biochemistry and Biophysics Reports,2018,13:109−115. doi: 10.1016/j.bbrep.2018.01.004
[17]	MOU K, LIU W, HAN D, et al. HMGB1/RAGE axis promotes autophagy and protects keratinocytes from ultraviolet radiation-induced cell death[J]. Journal of Dermatological Science,2017,85(3):162−169. doi: 10.1016/j.jdermsci.2016.12.011
[18]	BARALLA E, DEMONTIS M P, VARONI M V, et al. Bisphenol A and bisphenol S oxidative effects in sheep red blood cells: An in vitro study[J]. BioMed Research International, 2021, 2021.
[19]	VITALE N, KISSLINGER A, PALADINO S, et al. Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells[J]. PloS One,2013,8(11):e80728. doi: 10.1371/journal.pone.0080728
[20]	CHOI S H, CHOI S I, JUNG T D, et al. Anti-photoaging effect of jeju putgyul (unripe citrus) extracts on human dermal fibroblasts and ultraviolet B-induced hairless mouse skin[J]. International Journal of Molecular Sciences,2017,18(10):2052. doi: 10.3390/ijms18102052
[21]	SU Z Y, YANG Z Z, XU Y Q, et al. Apoptosis, autophagy, necroptosis, and cancer metastasis[J]. Molecular Cancer,2015,14(1):48. doi: 10.1186/1476-4598-14-1
[22]	FAZEKAS Z, GAO D, SALADI R N, et al. Protective effects of lycopene against ultraviolet B-induced photodamage[J]. Nutrition and Cancer,2003,47(2):181−187. doi: 10.1207/s15327914nc4702_11
[23]	CALÒ R, MARABINI L. Protective effect of vaccinium myrtillus extract against UVA-and UVB-induced damage in a human keratinocyte cell line (HaCaT cells)[J]. Journal of Photochemistry and Photobiology B:Biology,2014,132:27−35. doi: 10.1016/j.jphotobiol.2014.01.013
[24]	GEA B S, MORENO C B, DE LA CASA L, et al. Carotenoids from persimmon (Diospyros kaki Thunb.) byproducts exert photoprotective, antioxidative and microbial anti-adhesive effects on HaCaT[J]. Pharmaceutics,2021,13(11):1898. doi: 10.3390/pharmaceutics13111898
[25]	IMRAN M, GHORAT F, UL H I, et al. Lycopene as a natural antioxidant used to prevent human health disorders[J]. Antioxidants,2020,9(8):706. doi: 10.3390/antiox9080706
[26]	YOSHIHISA Y, REHMAN M U, SHIMIZU T. Astaxanthin, a xanthophyll carotenoid, inhibits ultraviolet-induced apoptosis in keratinocytes[J]. Experimental dermatology,2014,23(3):178−183. doi: 10.1111/exd.12347
[27]	SCHWAGER J, RICHARD N, MUSSLER B, et al. Tomato aqueous extract modulates the inflammatory profile of immune cells and endothelial cells[J]. Molecules,2016,21(2):168. doi: 10.3390/molecules21020168
[28]	TSOYI K, PARK H B, KIM Y M, et al. Anthocyanins from black soybean seed coats inhibit UVB-induced inflammatory cylooxygenase-2 gene expression and PGE₂ production through regulation of the nuclear factor-κB and phosphatidylinositol 3-kinase/Akt pathway[J]. Journal of Agricultural and Food Chemistry,2008,56(19):8969−8974. doi: 10.1021/jf801345c
[29]	HE Y, HU Y, JIANG X, et al. Cyanidin-3-O-glucoside inhibits the UVB-induced ROS/COX-2 pathway in HaCaT cells[J]. Journal of Photochemistry and Photobiology B: Biology,2017,177:24−31. doi: 10.1016/j.jphotobiol.2017.10.006
[30]	RODRÍGUEZ L A, ÁVILA R J, GONZÁLEZ-RODRÍGUEZ M L, et al. Fucoxanthin-containing cream prevents epidermal hyperplasia and UVB-induced skin erythema in mice[J]. Marine Drugs,2018,16(10):378. doi: 10.3390/md16100378
[31]	ALAM S, PAL A, SINGH D, et al. Topical application of nexrutine inhibits ultraviolet B-induced cutaneous inflammatory responses in SKH-1 hairless mouse[J]. Photodermatology, Photoimmunology & Photomedicine,2018,34(1):82−90.
[32]	VIYOCH J, MAHINGSA K, INGKANINAN K. Effects of thai musa species on prevention of UVB-induced skin damage in mice[J]. Food and Chemical Toxicology,2012,50(12):4292−4301. doi: 10.1016/j.fct.2012.08.060
[33]	MOON J M, PARK S H, JHEE K H, et al. Protection against UVB-induced wrinkle formation in SKH-1 hairless mice: Efficacy of tricin isolated from enzyme-treated zizania latifolia extract[J]. Molecules,2018,23(9):2254. doi: 10.3390/molecules23092254
[34]	AMER R I, EZZAT S M, ABOREHAB N M, et al. Downregulation of MMP1 expression mediates the anti-aging activity of Citrus sinensis peel extract nanoformulation in UV induced photoaging in mice[J]. Biomedicine & Pharmacotherapy,2021,138:111537.
[35]	HATA T R, SCHOLZ T A, PERSHING L K, et al. Non-invasive raman spectroscopic detection of carotenoids in human skin[J]. Journal of Investigative Dermatology,2000,115(3):441−448. doi: 10.1046/j.1523-1747.2000.00060.x
[36]	DE JAGER T L, COCKRELL A E, DU PLESSIS S S. Ultraviolet light induced generation of reactive oxygen species[J]. Ultraviolet Light in Human Health, Diseases and Environment, 2017: 15−23.
[37]	SIISKONEN H, SMORODCHENKO A, KRAUSE K, et al. Ultraviolet radiation and skin mast cells: Effects, mechanisms and relevance for skin diseases[J]. Experimental Dermatology,2018,27(1):3−8. doi: 10.1111/exd.13402
[38]	PENTLAND A P, MAHONEY M, JACOBS S C, et al. Enhanced prostaglandin synthesis after ultraviolet injury is mediated by endogenous histamine stimulation. A mechanism for irradiation erythema[J]. The Journal of Clinical Investigation,1990,86(2):566−574. doi: 10.1172/JCI114746
[39]	PRASANTH M I, GAYATHRI S, BHASKAR J P, et al. Understanding the role of p38 and JNK mediated MAPK pathway in response to UV-A induced photoaging in caenorhabditis elegans[J]. Journal of Photochemistry and Photobiology B: Biology,2020,205:111844. doi: 10.1016/j.jphotobiol.2020.111844
[40]	HUR S, LEE Y S, YOO H, et al. Homoisoflavanone inhibits UVB-induced skin inflammation through reduced cyclooxygenase-2 expression and NF-κB nuclear localization[J]. Journal of Dermatological Science,2010,59(3):163−169. doi: 10.1016/j.jdermsci.2010.07.001
[41]	MUTHUSAMY V, PIVA T J. The UV response of the skin: A review of the MAPK, NF kappaB and TNF alpha signal transduction pathways[J]. Archives of Dermatological Research,2010,302(1):5−17.

[1]	CHEN Yongfang, LI Yanke, ZHANG Shujing. Mechanism of Poria cocos Polysaccharide Regulating Autophagy and Chemotherapy Resistance of Hepatocellular Carcinoma Cells through LncRNAHCG11/miR-539-3p 539-3p[J]. Science and Technology of Food Industry, 2024, 45(22): 322-330. DOI: 10.13386/j.issn1002-0306.2023110291
[2]	LÜ Chenhao, LI Junjian, CHEN Chang'an, HE Zhilin, DU Bing, LI Pan. Anti-aging and in Vitro Antioxidant Effects of Water Extracts of Fermented Pericarpium Citri Reticulatae on Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2023, 44(17): 428-437. DOI: 10.13386/j.issn1002-0306.2022110162
[3]	YAN Jing, XUE Qiuyan, WANG Yang, CHEN Wenyi, XIE Shiqing, JIANG Jinjin, LI Pan, DU Bing. Hypolipidemic and Antioxidant Effects of Fermented Rice Buckwheat on High-fat Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2023, 44(6): 8-15. DOI: 10.13386/j.issn1002-0306.2022070044
[4]	WANG Gaojian, WANG Zhenzhen, LI Jiajia, FAN Haoan, SHA Ruyi, MAO Jianwei. Antioxidant Activity in Vitro and Promoting Resistance to Oxidative Stress in Caenorhabditis elegans of Blueberry Jiaosu[J]. Science and Technology of Food Industry, 2021, 42(15): 343-350. DOI: 10.13386/j.issn1002-0306.2020110157
[5]	LIU Chang, XU Yue, SHAN Cheng-ying, ZHU Chang-ling, ZHAO Fei, ZHANG Huan-shi. Exploring the Antioxidant Activity in Vivo of Coriander Extract in Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2020, 41(20): 285-289. DOI: 10.13386/j.issn1002-0306.2020.20.047
[6]	XU Jie, WANG Jun, TAN Xin-tong, LI Da-peng. Research Progress of Foodborne Flavonoids Interfering with Diseases by Regulating Autophagy[J]. Science and Technology of Food Industry, 2020, 41(16): 326-333. DOI: 10.13386/j.issn1002-0306.2020.16.052
[7]	SUN Yan, CUI Xu-sheng, LIU Jing, TIAN He, YANG Yan-fang, LI Ya-fen, BAO Jing-jing, LI Chun-lin, WANG Qing, ZHANG Yan-qing, XIE Jun-bo. Optimization of Extraction Process of Flavonoids from Ziziphus jujuba Mill var. spinosa Leaves and Its Antioxidant Damage Activity in Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2020, 41(8): 143-150. DOI: 10.13386/j.issn1002-0306.2020.08.023
[8]	XIE Yong-lei, CUI Ming-chen, HUANG Ya-nan, LI Pan-xin, XU Wan-ling, MA Yong-chao. Effects of Soybean Peptide on Haemodynamics and Myocardial Autophagy Levels in Rats with Exhaustive Exercise[J]. Science and Technology of Food Industry, 2019, 40(22): 316-320. DOI: 10.13386/j.issn1002-0306.2019.22.055
[9]	LIAO Xin, CHEN Wen-ying, LI Yi-zhou, LI Chen, LIU Xin-miao, SHEN Xiao-li. Oleanolic Acid Antagonized Autophagic Death of HEK293T Cells Induced by Ochratoxin A[J]. Science and Technology of Food Industry, 2019, 40(3): 286-289,295. DOI: 10.13386/j.issn1002-0306.2019.03.045
[10]	CHEN Jing-yao, ZHOU Jie, HAN Bin, LI Fei, ZHU Yan-feng, YU Xiao-ping. Delphinidin sensitizes anti-tumor effect to HER-2+breast cancer cells by combining with 3-MA[J]. Science and Technology of Food Industry, 2017, (02): 354-357. DOI: 10.13386/j.issn1002-0306.2017.02.060

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	杨宇恒，郑宇航，王文卓，刘芳，张新笑，孙芝兰. 真空包装鸡肉肠产气微生物分离鉴定及胀袋原因探析. 肉类研究. 2024(04): 36-42 .
2.	胡文静，刘小雪，梁栋，焦凌霞. 肌苷对酸土脂环酸芽孢杆菌生长及生物膜形成的影响. 中国食品学报. 2023(09): 242-251 .
3.	许育民，任兰兰，张颖，刘亚慧，王海花，张晓静，张晓峰. 抗食源性病原菌细菌素的筛选及特性研究. 食品安全质量检测学报. 2022(04): 1170-1175 .
4.	刘小杰，舒志成，赵志红，左迪. 调节血脂保健粥的研制. 食品工业科技. 2021(22): 240-245 . 本站查看