Anti-fatigue Effects of Mulberry Anthocyanins in Mice

YANG Shu; HUANG Xuying; TU Han; LIU Zhong; LIU Xin

doi:10.13386/j.issn1002-0306.2022090071

Volume 44 Issue 16

Aug. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(16): 377-385. > DOI: 10.13386/j.issn1002-0306.2022090071

YANG Shu, HUANG Xuying, TU Han, et al. Anti-fatigue Effects of Mulberry Anthocyanins in Mice[J]. Science and Technology of Food Industry, 2023, 44(16): 377−385. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090071.

Citation:

PDF (1511 KB)

Anti-fatigue Effects of Mulberry Anthocyanins in Mice

Pharmaceutical Preparation Section, Wuhan Fourth Hospital, Wuhan 430034, China

More Information

Received Date: September 07, 2022
Available Online: June 12, 2023

Graphical Abstract

Abstract

Abstract

Objective: To study the anti-fatigue effects of mulberry anthocyanins (AM) and discuss the mechanism in mice. Methods: After preparation and analysis, the the antioxidant activity AM was evaluated. The mice were randomly divided into the quiet group, the aerobic exercise group, the model group, the low, medium and high does groups (the AM dosage were 100, 200 and 400 mg/kg/d, respectively), and exhaustive swimming test were performed. After mouse were orally administered for 4 weeks, the fatigue-related biochemical indicators were measured to evaluate the anti-fatigue effect of AM, such as the levels of blood lactic acid (Lac), blood urea nitrogen (BUN) and malondialdehyde (MDA) the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Meanwhile, the anti-fatigue mechanism was explained by the relative expression levels of Nrf2 and HO-1 mRNA. Result: After enrichment and purification, the contents of active components in AM were increased, and showed the antioxidant capacity that equivalented to vitamin C. Compared with the model group, the low, medium and high does groups were significantly prolonged exhaustive swimming time (P<0.05), as well as the exercise capacity was increased. The medium and high does of AM significantly decreased the levels of Lac and BUN (P<0.05), the activities of LDH and CK (P<0.05) in rat blood, improved the activity of SOD and GSH-Px, as well as low-regulate the level of MDA, 8-OHdG and ROS (P<0.05) in muscle. Furthermore, AM significantly increased the expression levels of Nrf2/HO-1 mRNA in muscle (P<0.05). Conclusion: AM could improve the ability of the antioxidant capacity to exert anti fatigue effect through regulating Nrf2/HO-1 signaling pathway.
- mulberry,
- anthocyanins,
- anti-fatigue,
- antioxidant,
- oxidative stress

FullText(HTML)

References (35)

References

[1]	ZHOU S S, JIANG J G. Anti-fatigue effects of active ingredients from traditional Chinese medicine: a review[J]. Current Medicinal Chemistry,2019,26(10):1833−1848. doi: 10.2174/0929867324666170414164607
[2]	PENG X, GAO L, AIBAI S. Antifatigue effects of Anshenyizhi compound in acute excise-treated mouse via modulation of AMPK/PGC-1α-related energy metabolism and Nrf2/ARE-mediated oxidative stress[J]. Journal of Food Science,2020,85(6):1897−1906. doi: 10.1111/1750-3841.15149
[3]	康鹏, 李国薇, 马宏祥, 等. 运动营养食品及其抗疲劳活性成分研究进展[J]. 食品安全质量检测学报,2021,12(23):9157−9164. [KANG P, LI G W, MA H Y, et al. Research progress of sports nutrition food and its anti-fatigue bioactive components[J]. Journal of Food Safety and Quality,2021,12(23):9157−9164. doi: 10.3969/j.issn.2095-0381.2021.23.spaqzljcjs202123025 KANG P, LI G W, MA H Y, et al. Research progress of sports nutrition food and its anti-fatigue bioactive components[J]. Journal of Food Safety and Quality, 2021, 12(23): 9157-9164. doi: 10.3969/j.issn.2095-0381.2021.23.spaqzljcjs202123025
[4]	靳铁柱. 响应面法优化桑葚多糖纯化工艺及不同产物的抗运动疲劳活性比较[J]. 保鲜与加工,2022,22(4):67−73. [JIN T Z. Optimization of purification process of mulberry polysaccharide by response surface methodology and comparison of anti-exercise fatigue activity of different products[J]. Storage and Process,2022,22(4):67−73. doi: 10.3969/j.issn.1009-6221.2022.04.009 JIN T Z. Optimization of purification process of mulberry polysaccharide by response surface methodology and comparison of anti-exercise fatigue activity of different products[J]. Storage and Process, 2022, 22(4): 67-73. doi: 10.3969/j.issn.1009-6221.2022.04.009
[5]	马永昆, 许满青, 陈必祥, 等. 桑椹果醋营养功能功效解读[J]. 食品安全质量检测学报,2021,12(6):2117−2124. [MA Y K, XU M Q, CHEN B X, et al. Interpretation of the nutritional function of mulberry vinegar[J]. Journal of Food Safety and Quality,2021,12(6):2117−2124. doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.06.011 MA Y K, XU M Q, CHEN B X, et al. Interpretation of the nutritional function of mulberry vinegar[J]. Journal of Food Safety and Quality, 2021, 12(6): 2117-2124. doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.06.011
[6]	马识淳, 韩伟. 襄荷桑葚复合饮料研制及其抗运动疲劳作用研究[J]. 中国食品添加剂,2021,32(8):67−75. [MA S C, HAN W. Development and anti-fatigue effect of compound beverage of Zingiber strioatum and mulberry[J]. China Food Additive,2021,32(8):67−75. doi: 10.19804/j.issn1006-2513.2021.08.010 MA S C, HAN W. Development and anti-fatigue effect of compound beverage of Zingiber strioatum and mulberry[J]. China Food Additive, 2021, 32(8): 67-75. doi: 10.19804/j.issn1006-2513.2021.08.010
[7]	薛宏坤, 李鹏程, 钟雪, 等. 高速逆流色谱分离纯化桑葚花色苷及其抗氧化活性[J]. 食品科学,2020,41(15):96−104. [XUE H K, LI P C, ZHONG X, et al. Separation and purification of anthocyanins from mulberry fruit by high-speed counter-current chromatography and their antioxidant activity[J]. Food Science,2020,41(15):96−104. doi: 10.7506/spkx1002-6630-20190715-193 XUE H K, LI P C, ZHONG X, et al. Separation and purification of anthocyanins from mulberry fruit by high-speed counter-current chromatography and their antioxidant activity[J]. Food Science, 2020, 41(15): 96-104. doi: 10.7506/spkx1002-6630-20190715-193
[8]	FANG J L, JIA S S, LIN Y, et al. Extraction, purification, content analysis and hypoglycemic effect of mulberry marc anthocyanin[J]. Pharmacognosy Magazine,2020,16:68−75. doi: 10.4103/pm.pm_169_19
[9]	赵秀玲, 范道春. 桑椹的生理活性成分、提取检测及药理作用研究进展[J]. 药物分析杂志,2017,37(3):378−385. [ZHAO X L, FAN D C. Review of physiological active components, extraction and detection methods and pharmacological bioactivities of mulberry[J]. Chinese Journal of Pharmaceutical Analysis,2017,37(3):378−385. doi: 10.16155/j.0254-1793.2017.03.02 ZHAO X L, FAN D C. Review of physiological active components, extraction and detection methods and pharmacological bioactivities of mulberry[J]. Chinese Journal of Pharmaceutical Analysis, 2017, 37(3): 378-385. doi: 10.16155/j.0254-1793.2017.03.02
[10]	罗晓玲, 徐嘉红, 杨武斌, 等. 蓝莓花色苷抗氧化功能及稳定性研究进展[J]. 食品工业科技,2018,39(4):312−317. [LUO X L, XU J H, YANG W B, et al. Research progress in antioxidant function and stability of blueberry anthocyanins[J]. Science and Technology of Food Industry,2018,39(4):312−317. doi: 10.13386/j.issn1002-0306.2018.04.057 LUO X L, XU J H, YANG W B, et al. Research progress in antioxidant function and stability of blueberry anthocyanins[J]. Science and Technology of Food Industry, 2018, 39(4): 312-317. doi: 10.13386/j.issn1002-0306.2018.04.057
[11]	YANG S, WAN C, LI X Y, et al. Investigation on the biological activity of anthocyanins and polyphenols in blueberry[J]. Food Science,2021,86(2):614−627. doi: 10.1111/1750-3841.15598
[12]	WU T, YIN J, ZHANG G, et al. Mulberry and cherry anthocyanin consumption prevents oxidative stress and inflammation in diet-induced obese mice[J]. Molecular Nutrition & Food Research,2016,60:687−694.
[13]	CHEN Y, DU F, WANG W, et al. Large-scale isolation of high-purity anthocyanin monomers from mulberry fruits by combined chromatographic techniques[J]. Journal of Separation Science,2017,40(17):3506−3512. doi: 10.1002/jssc.201700471
[14]	ZHOU Y J, SONG J L, XU Q, et al. Optimization and application of HPLC for simultaneous separation of six well-known major anthocyanins in blueberry[J]. Preparative Biochemistry & Biotechnology,2021,51(10):961−970.
[15]	SURIYAPROM S, KAEWKOD T, PROMPUTTHA I, et al. Evaluation of antioxidant and antibacterial activities of white mulberry (Morus alba L. ) fruit extracts[J]. Plants,2021,10(12):2736. doi: 10.3390/plants10122736
[16]	郑传痴, 杨艳, 韦余, 等. 金丝桃苷对小鼠的抗疲劳作用及机制研究[J]. 食品工业科技,2021,42(23):350−355. [ZHENG C C, YAN Y, WEI Y, et al. Study on the effects and mechanism of hyperoside on anti-fatigue in mice[J]. Science and Technology of Food Industry,2021,42(23):350−355. doi: 10.13386/j.issn1002-0306.2021010227 ZHENG C C, YAN Y, WEI Y, et al. Study on the effects and mechanism of hyperoside on anti-fatigue in mice[J]. Science and Technology of Food Industry, 2021, 42(23): 350-355. doi: 10.13386/j.issn1002-0306.2021010227
[17]	程美玲, 黄鑫, 安曈昕, 等. 玫瑰花青素对小鼠抗疲劳及抗氧化研究[J]. 云南农业大学学报(自然科学),2021,36(6):956−961. [CHEN M L, HUANG X, AN T X, et al. Study on anti-fatigue and anti-oxidation effects of rose anthocyanin in mice[J]. Journal of Yunnan Agricultural University (Natural Science),2021,36(6):956−961. CHEN M L, HUANG X, AN T X, et al. Study on anti-fatigue and anti-oxidation effects of rose anthocyanin in mice[J]. Journal of Yunnan Agricultural University (Natural Science), 2021, 36(6): 956-961.
[18]	ZHANG L, FAN G, KHAN M A, et al. Ultrasonic-assisted enzymatic extraction and identification of anthocyanin components from mulberry wine residues[J]. Food Chemistry,2020,323:126714. doi: 10.1016/j.foodchem.2020.126714
[19]	ZHANG S, LIU B, YAN G, et al. Chemical properties and anti-fatigue effect of polysaccharide from Pholiota nameko[J]. Journal of Food Biochemistry,2022,46:e14015.
[20]	MA C, DENG Y, XIAO R, et al. Anti-fatigue effect of phlorizin on exhaustive exercise-induced oxidative injury mediated by Nrf2/ARE signaling pathway in mice[J]. European Journal of Pharmacology,2022,918:174563. doi: 10.1016/j.ejphar.2021.174563
[21]	LI Y, DENG Y, LI Z, et al. Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins[J]. International Journal of Biological Macromolecules,2020,165:2630−2639. doi: 10.1016/j.ijbiomac.2020.10.167
[22]	QIAO Y B, YE Y, CAI T X, et al. Anti-fatigue activity of the polysaccharides isolated from Ribes stenocarpum Maxim[J]. Journal of Functional Foods,2022,89:104947. doi: 10.1016/j.jff.2022.104947
[23]	HU M, DU J, DU L D, et al. Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim.) epicarp in mice[J]. Journal of Functional Foods,2020,65:103725. doi: 10.1016/j.jff.2019.103725
[24]	JIN S H, CAI R R, CHENG F R, et al. Content determination and anti-fatigue effect of the purified anthocyanin from purple Daucus carota[J]. Pharmacognosy Magazine,2020,16(71):670−674. doi: 10.4103/pm.pm_452_19
[25]	HE W, GUO F, JIANG Y, et al. Enzymatic hydrolysates of soy protein promote the physicochemical stability of mulberry anthocyanin extracts in food processing[J]. Food Chemistry,2022,386:132811. doi: 10.1016/j.foodchem.2022.132811
[26]	ZHU H, XU W, WANG N, et al. Anti-fatigue effect of Lepidium meyenii Walp. (Maca) on preventing mitochondria-mediated muscle damage and oxidative stress in vivo and vitro[J]. Food Function,2021,12(7):3132−3141. doi: 10.1039/D1FO00383F
[27]	HAO J, GAO Y, XUE J, et al. Phytochemicals, pharmacological effects and molecular mechanisms of mulberry[J]. Foods,2022,11(8):1170. doi: 10.3390/foods11081170
[28]	CHEN T, SHUANG F F, FU Q Y, et al. Evaluation of the chemical composition and antioxidant activity of mulberry (Morus alba L.) fruits from different varieties in China[J]. Molecules,2022,27(9):2688. doi: 10.3390/molecules27092688
[29]	ZHU S, YANG W, LIN Y, et al. Antioxidant and anti-fatigue activities of selenium-enriched peptides isolated from Cardamine violifolia protein hydrolysate[J]. Journal of Functional Foods,2021,79:104412. doi: 10.1016/j.jff.2021.104412
[30]	ZHANG X, JING S, LIN H, et al. Anti-fatigue effect of anwulignan via the NRF2 and PGC-1α signaling pathway in mice[J]. Food Function,2019,10(12):7755−7766. doi: 10.1039/C9FO01182J
[31]	张馨芸, 林慧娇, 李欣, 等. 五味子酯甲通过调节肝脏Nrf2/ARE抗氧化通路改善小鼠疲劳的作用[J]. 食品科学,2020,41(1):190−195. [ZHANG X Y, LIN H Q, LI X, et al. Schisantherin a improves fatigue in mice by regulating the Nrf2/ARE antioxidant pathway in liver[J]. Food Science,2020,41(1):190−195. doi: 10.7506/spkx1002-6630-20181120-230 ZHANG X Y, LIN H Q, LI X, et al. Schisantherin a improves fatigue in mice by regulating the Nrf2/ARE antioxidant pathway in liver[J]. Food Science, 2020, 41(1): 190-195. doi: 10.7506/spkx1002-6630-20181120-230
[32]	蓝瑞高, 梁益军. 人参皂苷CK对力竭游泳大鼠抗疲劳作用及骨骼肌氧化应激的影响[J]. 云南农业大学学报(自然科学),2022,37(3):491−496. [LAN R G, LIANG Y J. Effects of ginsenoside CK on anti-fatigue and oxidative stress of skeletal muscle in exhaustive swimming rats[J]. Journal of Yunnan Agricultural University (Natural Science),2022,37(3):491−496. doi: 10.12101/j.issn.1004-390X(n).202109040 LAN R G, LIANG Y J. Effects of ginsenoside CK on anti-fatigue and oxidative stress of skeletal muscle in exhaustive swimming rats[J]. Journal of Yunnan Agricultural University (Natural Science), 2022, 37(3): 491-496. doi: 10.12101/j.issn.1004-390X(n).202109040
[33]	BAI X, LIAN Y, HU C, et al. Cyanidin-3-glucoside protects against high glucose-induced injury in human nucleus pulposus cells by regulating the Nrf2/HO-1 signaling[J]. Journal of Applied Toxicology,2022,42(7):1137−1145. doi: 10.1002/jat.4281
[34]	SONG Y, HUANG L, YU J. Effects of blueberry anthocyanins on retinal oxidative stress and inflammation in diabetes through Nrf2/HO-1 signaling[J]. Journal of Neuroimmunology,2016,301:1−6. doi: 10.1016/j.jneuroim.2016.11.001
[35]	ALI T, KIM T, REHMAN S U, et al. Natural dietary supplementation of anthocyanins via PI3K/Akt/Nrf2/HO-1 pathways mitigate oxidative stress, neurodegeneration, and memory impairment in a mouse model of Alzheimer's disease[J]. Molecular Neurobiology,2018,55(7):6076−6093. doi: 10.1007/s12035-017-0798-6

Supplements (0)

Cited By

Cited by

Periodical cited type(3)

1.	Tiantian Xu，Zheng Yang，Shichao Xie，Tingting Zhu，Wenli Zhao，Min Jin，Qicun Zhou. Evaluation of cottonseed oil as a substitute for fish oil in the commercial diet for juvenile swimming crabs(Portunus trituberculatus). Animal Nutrition. 2024(04): 466-479 .
2.	冼才凝，范立成，郭学骞，王锡昌. 渔光一体养殖模式对中华绒螯蟹肝胰腺和性腺气味品质的影响. 渔业科学进展. 2023(03): 209-221 .
3.	董妍玉，张莹，苏仁敬，窦志英，何俊，王晖. 基于生姜炮制品研究和GC-MS验证的气味分析仪应用性探讨. 中国中药杂志. 2022(24): 6633-6640 .