Effects of <i>Tetrastigma hemsleyanum</i> Superfine Powder on Intestinal Microflora in Rats with Alcohol-Induced Liver Injury

CHENG Xiaoyang; LIAO Ming; HE Quanguang; MO Caifeng; HUANG Maokang; HUANG Meihua

doi:10.13386/j.issn1002-0306.2022090022

Volume 44 Issue 18

Sep. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(18): 415-424. > DOI: 10.13386/j.issn1002-0306.2022090022

CHENG Xiaoyang, LIAO Ming, HE Quanguang, et al. Effects of Tetrastigma hemsleyanum Superfine Powder on Intestinal Microflora in Rats with Alcohol-Induced Liver Injury[J]. Science and Technology of Food Industry, 2023, 44(18): 415−424. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090022.

Citation:

PDF (5227 KB)

Effects of Tetrastigma hemsleyanum Superfine Powder on Intestinal Microflora in Rats with Alcohol-Induced Liver Injury

1.
Guangxi Medical University, Nanning 530021, China
2.
Dinghu People's Hospital, Zhaoqing 526070, China
3.
Institute of Agro-products Processing and Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
4.
Guangxi Huayin Medical Laboratory Center, Nanning 530000, China

More Information

Received Date: September 04, 2022
Available Online: July 20, 2023

Graphical Abstract

Abstract

Abstract

Objective: To investigate and reveal protective mechanism which the Tetrastigma hemsleyanum (SYQ) ultramicro powder alleviate alcohol-induced liver injury, and to provide a new research idea for clinical treatment of alcohol-induced liver injury. Methods: The rat model of alcoholic liver injury was established by continuous administration of alcohol for 8 weeks. At the same time, rats were given different doses SYQ ultramicro powder by intragastric to observe its protective effect on liver injury. Changes of ALT, AST, ALP and GSH, SOD, MDA were detected in serum and liver tissue, respectively. Pathological sections of liver tissue were used to observe the damage. Genomic DNA was extracted from feces and analyzed by 16S rDNA full-length sequencing. Results: Compared with the model group, medium and high doses of SYQ (0.5 and 1.0 g·kg⁻¹) significantly (P<0.05) reduced the levels of ALT, AST ALP and MDA, increased the activities of SOD and GSH, and alleviated the liver lesions in rats. The pathological sections revealed that SYQ ultramicro powder dramatically alleviated liver cells swelling and steatosis, and these effectiveness were similar to the group of bifendate. Intestinal microflora analysis showed that the intestinal microflora structure significantly changed in rats with alcoholic liver injury. Compared with the model group, both SYQ and bifendate intervention improved the intestinal flora to a certain extent at phylum, genus and species level. SYQ effectively recovered the abundance of Prevotella, Lactobacillus_johnsonii, Raoultibacter_massiliensis altered by alcohol, and the effect of medium doses of SYQ was better than positive control group (P<0.05). Conclusion: The SYQ superfine powder had a significant protective effect on alcoholic-induced liver injury in rats, the mechanism may be related to its antioxidant stress and intestinal flora regulation.
- Tetrastigma hemsleyanum,
- superfine powder,
- alcohol-induced liver injury,
- oxidative stress,
- intestinal microflora

FullText(HTML)

References (38)

References

[1]	TORRUELLAS C, FRENCH S W, MEDICI V. Diagnosis of alcoholic liver disease[J]. World J Gastroenterol,2014,20(33):11684−11699. doi: 10.3748/wjg.v20.i33.11684
[2]	CHAYANUPATKUL M, LIANGPUNSAKUL S. Alcoholic hepatitis: A comprehensive review of pathogenesis and treatment[J]. World J Gastroenterol,2014,20(20):6279−6286. doi: 10.3748/wjg.v20.i20.6279
[3]	KOURKOUMPETIS T, SOOD G. Pathogenesis of alcoholic liver disease: An update[J]. Clinics in Liver Disease,2019,23(1):71−80. doi: 10.1016/j.cld.2018.09.006
[4]	臧月, 王生, 刘楠, 等. 肠道菌群失调介导酒精性肝病发生发展的机制研究进展[J]. 中国药理学通报,2016,32(4):451−454. [ZANG Y, WANG S, LIU N, et al. Alcoholic liver disease: gut microbiota and therapeutic perspectives[J]. Chinese Pharmacological Bulletin,2016,32(4):451−454. ZANG Y, WANG S, LIU N, et al. Alcoholic liver disease: gut microbiota and therapeutic perspectives[J]. Chinese Pharmacological Bulletin, 2016, 32(4): 451-454.
[5]	DING F, LIU J, DU R, et al. Qualitative and quantitative analysis for the chemical constituents of Tetrastigma hemsleyanum Diels et Gilg using ultra-high performance liquid chromatography/hybrid quadrupole-orbitrap mass spectrometry and preliminary screening for anti-influenza virus components[J]. Evid Based Complement Alternat Med,2019,2019:9414926.
[6]	黄真, 毛庆秋. 三叶青总氨基酸对四氯化碳致小鼠肝损伤的保护作用[J]. 中国现代应用药学,2007,24(3):190−192. [HUANG Z, MAO Q Q. Protective effects of total amino acids from Radix Tetrastigmae on acute hepatic injury induced by CCl₄[J]. Chinese Journal of Modern Applied Pharmacy,2007,24(3):190−192. HUANG Z, MAO Q Q. Protective effects of total amino acids from Radix Tetrastigmae on acute hepatic injury induced by CCl₄[J]. Chinese Journal of Modern Applied Pharmacy, 2007, 24(3): 190-192.
[7]	李萍, 吉薇薇, 彭昕. 三叶青提取物抗ANIT所致小鼠肝损伤作用的研究[J]. 中国现代医生,2018,56(30):32−35,169. [LI P, JI W W, PENG X. Study on the trifolium extract in anti ANIT-induced liver injury in mice[J]. China Modern Doctor,2018,56(30):32−35,169. LI P, JI W W, PENG X. Study on the trifolium extract in anti ANIT-induced liver injury in mice[J]. China Modern Doctor, 2018, 56(30): 32-35,169.
[8]	李萍, 彭昕, 楼天灵, 等. 三叶青多糖抗免疫性肝损伤的作用机制研究[J]. 中药材,2020,43(3):712−715. [LI P, PENG X, LOU T L, et al. Mechanism of Radix Tetrastigmae polysaccharides against immune liver injury[J]. Journal of Chinese Medicinal Materials,2020,43(3):712−715. LI P, PENG X, LOU T L, et al. Mechanism of Radix Tetrastigmae polysaccharides against immune liver injury[J]. Journal of Chinese Medicinal Materials, 2020, 43(3): 712-715.
[9]	钟晓明, 毛庆秋, 黄真, 等. 三叶青提取物对四氯化碳致急性肝损伤小鼠的保护作用及急性毒性实验[J]. 中成药,2006,28(3):422−424. [ZHONG X M, MAO Q Q, HUANG Z, et al. Protective effects and acute toxicity experiments of Radix Tetrastigmae extract on acute liver injury induced by CCl₄ in mice[J]. Chinese Traditional Patent Medicine,2006,28(3):422−424. ZHONG X M, MAO Q Q, HUANG Z, et al. Protective effects and acute toxicity experiments of Radix Tetrastigmae extract on acute liver injury induced by CCl₄ in mice[J]. Chinese Traditional Patent Medicine, 2006, 28(3): 422-424.
[10]	范蕾, 鞠爱华, 刘丽娟, 等. 蒙药大栀子超微粉与原粉抗炎、镇痛作用对比[J]. 中药药理与临床,2015,31(6):109−111. [FAN L, JÜ A H, LIU L J, et al. The comparative study of anti-inflammatory and analgesic effect between ultramicro powder and fine powder of Mongolian medicine gardenia[J]. Pharmacology and Clinics of Chinese Materia Medica,2015,31(6):109−111. FAN L, JU A H, LIU L J, et al. The comparative study of anti-inflammatory and analgesic effect between ultramicro powder and fine powder of Mongolian medicine Gardenia[J]. Pharmacology and Clinics of Chinese Materia Medica, 2015, 31(6): 109-111.
[11]	靳子明, 宋治荣, 窦霞. 党参超微粉对胃溃疡模型大鼠胃黏膜保护作用的研究[J]. 中国现代应用药学,2017,34(5):659−661. [JIN Z M, SONG Z R, DOU X. Protective effects of codonopsis radix on gastric mucosa in rats with gastric ulcer[J]. Chinese Journal of Modern Applied Pharmacy,2017,34(5):659−661. JIN Z M, SONG Z R, DOU X. Protective effects of codonopsis radix on gastric mucosa in rats with gastric ulcer[J]. Chinese Journal of Modern Applied Pharmacy, 2017, 34(5): 659-661.
[12]	武炜. 菊苣根超微粉对免疫抑制小鼠肠道菌群的影响[J]. 天然产物研究与开发,2016,28(3):429−432. [WU W. Effect of superfine powder of Cichorium intybus L. root on intestinal microflora in immunosuppressive mice[J]. Natural Product Research and Development,2016,28(3):429−432. WU W. Effect of superfine powder of Cichorium intybus L. root on intestinal microflora in immunosuppressive mice[J]. Natural Product Research and Development, 2016, 28(3): 429-432.
[13]	杨章坚, 徐子金, 叶锡勇, 等. 中药三叶青专利技术分析[J]. 科技视界,2017(24):49−50. [YANG Z J, XU Z J, YE X Y, et al. Patent technology analysis of Chinese traditional medicine Tetrastigma hemsleyanum[J]. Science and Technology Horizon,2017(24):49−50. YANG Z J, XU Z J, YE X Y, et al. Patent technology analysis of Chinese traditional medicine Tetrastigma hemsleyanum[J]. Science and Technology Horizon, 2017(24): 49-50.
[14]	刘明, 陈绍红, 钟赣生, 等. 葛花枳椇子配伍对酒精性肝损伤大鼠肝脏功能及病理形态的影响[J]. 南京中医药大学学报,2015,31(2):147−151. [LIU M, CHEN S H, ZHONG G S, et al. Effects of flos puerariae-semen hoveniae compatibility on liver function and pathologic morphology in rats with alcoholic liver disease[J]. Journal of Nanjing University of Traditional Chinese Medicine,2015,31(2):147−151. LIU M, CHEN S H, ZHONG G S, et al. Effects of flos puerariae-semen hoveniae compatibility on liver function and pathologic morphology in rats with alcoholic liver disease[J]. Journal of Nanjing University of Traditional Chinese Medicine, 2015, 31(2): 147-151.
[15]	刘泽鑫, 刘畅, 钱和. 白首乌花多糖对小鼠酒精性肝损伤的保护作用[J]. 食品研究与开发,2020,41(13):72−79. [LIU Z X, LIU C, QIAN H. Protective effect of cynanchum auriculatum flower polysaccharide on alcoholic liver injury in mice[J]. Food Research and Development,2020,41(13):72−79. LIU Z X, LIU C, QIAN H. Protective effect of cynanchum auriculatum flower polysaccharide on alcoholic liver injury in mice[J]. Food Research and Development, 2020, 41(13): 72-79.
[16]	朱诗雅. 具有缓解慢性酒精性肝损伤功能的乳杆菌筛选与功效评价[D]. 无锡: 江南大学, 2020. ZHU S Y. Screening and functional evaluation of Lactobacillus for alleviating chronic alcoholic liver injury[D]. Wuxi: Jiangnan University, 2020.
[17]	SHIN J, LEE S, GO M J, et al. Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing[J]. Scientific Reports,2016(6):29681.
[18]	BERESWILL S, EKMEKCIU I, ESCHER U, et al. Lactobacillus johnsonii ameliorates intestinal, extra-intestinal and systemic pro-inflammatory immune responses following murine Campylobacter jejuni infection[J]. Scientific Reports,2017(7):2138.
[19]	CHAGWEDERA D N, ANG Q Y, BISANZ J E, et al. Nutrient sensing in CD11c cells alters the gut microbiota to regulate food intake and body mass[J]. Cell Metabolism,2019(30):364−373.
[20]	FONSECA W, MALINCZAK C A, FUJIMURA K, et al. Maternal gut microbiome regulates immunity to RSV infection in offspring[J]. 2021, 218(11): e20210235.
[21]	FUNG T C, VUONG H E, LUNA C D G, et al. Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut[J]. Nature Microbiology,2019(4):2064−2073.
[22]	LOUVET A, MATHURIN P. Alcoholic liver disease: mechanisms of injury and targeted treatment[J]. Nature Reviews Gastroenterology & Hepatology,2015,12(4):231−42.
[23]	PAZ A L, HAO F, NELSON L J, et al. Alcoholic liver disease: Utility of animal models[J]. World J Gastroenterol,2018,24(45):5063−5075. doi: 10.3748/wjg.v24.i45.5063
[24]	DIEHL A M. Liver disease in alcohol abusers: clinical perspective[J]. Alcohol,2002,27(1):7−11. doi: 10.1016/S0741-8329(02)00204-5
[25]	JI W W, PENG X, LOU T L, et al. Total flavonoids from Tetrastigma hemsleyanum ameliorates inflammatory stress in concanavalin A-induced autoimmune hepatitis mice by regulating Treg/Th17 immune homeostasis[J]. Inflammopharmacology,2019,27(12):1297−1307.
[26]	谢阶飞, 伍昭龙, 罗志华, 等. 三叶青抗肝病变药效研究及临床应用的回顾与展望[J]. 中国民族民间医药,2008,17(9):28−30. [XIE J F, WU Z L, LUO Z H, et al. Research review on drug effects and clinical application of Tetrastigmatis hems leyani in liver disease prevention[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy,2008,17(9):28−30. XIE J F, WU Z L, LUO Z H, et al. Research review on drug effects and clinical application of Tetrastigmatis hems leyani in liver disease prevention[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2008, 17(9): 28-30.
[27]	RU Y, CHEN X, WANG J, et al. Polysaccharides from Tetrastigma hemsleyanum Diels et Gilg: Extraction optimization, structural characterizations, antioxidant and antihyperlipidemic activities in hyperlipidemic mice[J]. International Journal of Biological Macromolecules,2019,125:1033−1041. doi: 10.1016/j.ijbiomac.2018.11.236
[28]	CHEN S, LUO M, MA L, et al. Ethylacetate extract from Tetrastigma hemsleyanum inhibits proliferation and induces apoptosis in HepG2 and SMMC-7721 cells[J]. Cancer Management and Research,2018,10:3793−3799. doi: 10.2147/CMAR.S168333
[29]	PENG X, ZHANG Y Y, WANG J, et al. Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells[J]. Tumour Biol,2016,37(1):865−876. doi: 10.1007/s13277-015-3579-8
[30]	张鹏, 刘瑢, 张霞, 等. 秦艽对小鼠酒精性肝损伤的保护作用[J]. 中国医院药学杂志,2014,34(21):1822−1825. [ZHANG P, LIU R, ZHANG X, et al. Protective effect of Gentiana macrophylla Pall against liver injury induced by alcohol in mice[J]. Chinese Journal of Hospital Pharmacy,2014,34(21):1822−1825. ZHANG P, LIU R, ZHANG X, et al. Protective effect of Gentiana macrophylla Pall against liver injury induced by alcohol in mice[J]. Chinese Journal of Hospital Pharmacy, 2014, 34(21): 1822-1825.
[31]	SHA L, TAN H Y, WANG N, et al. The role of oxidative stress and antioxidants in liver diseases[J]. International Journal of Molecular Sciences,2015,16(11):26087−26124. doi: 10.3390/ijms161125942
[32]	CENI E, MELLO T, GALLI A. Pathogenesis of alcoholic liver disease: role of oxidative metabolism[J]. World J Gastroenterol,2014,20(47):17756−17772. doi: 10.3748/wjg.v20.i47.17756
[33]	XIAO J, ZHANG R F, ZHOU Q Y, et al. Lychee (Litchi chinensis Sonn. ) pulp phenolic extract provides protection against alcoholic liver injury in mice by alleviating intestinal microbiota dysbiosis, intestinal barrier dysfunction, and liver inflammation[J]. Journal of Agricultural and Food Chemistry,2017,65(44):9675−9684. doi: 10.1021/acs.jafc.7b03791
[34]	BULL OTTERSON L, FENG W, KIRPICH I, et al. Metagenomic analyses of alcohol induced pathogenic alterations in the intestinal microbiome and the effect of Lactobacillus rhamnosus GG treatment[J]. PloS One,2013,8(1):e53028. doi: 10.1371/journal.pone.0053028
[35]	CHEN P, TORRALBA M, TAN J, et al. Supplementation of saturated long-chain fatty acids maintains intestinal eubiosis and reduces ethanol-induced liver injury in mice[J]. Gastroenterology,2015,148(1):203−214. doi: 10.1053/j.gastro.2014.09.014
[36]	HERBERT T, PATRICE D C, EMERAN A M. Gut microbiome and liver diseases[J]. Basic Science,2016,65:2035−2044.
[37]	GRANDER C, ADOLPH T E, WIESER V, et al. Recovery of ethanol-induced Akkermansia muciniphila depletion ameliorates alcoholic liver disease[J]. Gut: Journal of the British Society of Gastroenterology,2018,67(5):891−901.
[38]	王文成. 海兔素对慢性酒精性肝损伤大鼠TLR4信号转导通路和肠道菌群的影响[D]. 青岛: 青岛大学, 2016. WANG W C. Effects of Aplysin on TLR4 signaling pathway and intestinal floras in rats with chronic alcohol-induced liver damage[D]. Qingdao: Qingdao University, 2016.

Supplements (0)

Cited By

Cited by

Periodical cited type(12)

1.	崔蓬勃，梁健亲，程天宇，吕飞，丁玉庭. 日本鲭的保鲜技术研究进展. 水产学报. 2024(07): 3-17 .
2.	罗振玲，高海波，杨挺，付余. 超高效液相色谱-串联质谱法同时测定小黄花鱼中9种生物胺. 食品工业科技. 2023(05): 251-257 . 本站查看
3.	杨梓璐，石懿平，郑火建，李洪彪，汪立平. 产胺菌拮抗菌的筛选鉴定及其抑菌物质特性研究. 湖北民族大学学报(自然科学版). 2023(03): 308-313+330 .
4.	黄岩，鲜双，李倩，陈其青，徐飞，陈安均. 豇豆泡菜中产生物胺菌株的筛选鉴定及其产胺特性研究. 食品与发酵工业. 2023(23): 119-126 .
5.	高建操，杜金梁，邵乃麟，张幸，李昺之，宋超，徐跑，徐钢春. 中华绒螯蟹质量安全新兴检测与控制技术研究进展. 中国渔业质量与标准. 2022(04): 62-70 .
6.	杨姗姗，王晓雯，林翠苹. 水产品中生物胺的研究进展. 青岛农业大学学报(自然科学版). 2021(01): 65-73 .
7.	王纯纯，刘智禹，黄鹭强. 水产品中微生物产胺的研究概况. 福建轻纺. 2021(11): 8-12 .
8.	李璇，刘琪，朱蔚姗，陈静，张佩娜，蒋立文. 不同发酵豆制品中生物胺调查分析. 食品安全质量检测学报. 2020(01): 298-305 .
9.	李少丽，邓建朝，李春生，杨贤庆，吴燕燕，陈胜军，马海霞. 生食大眼金枪鱼中生物胺产生菌的分离与鉴定. 食品与发酵工业. 2020(14): 121-126 .
10.	孙项丽，王联珠，郭莹莹，江艳华，王婧媛，尹大芳. 不同储藏温度下鲅鱼组胺含量与其品质变化的关系. 南方农业学报. 2020(08): 2005-2012 .
11.	孙项丽，郭莹莹，于秀娟，王静媛，文艺晓，王联珠. 鲭鱼品质评价及品质变化与组胺含量研究进展. 食品安全质量检测学报. 2019(12): 3708-3713 .
12.	邓建朝，李少丽，杨贤庆，陈胜军，吴燕燕，李春生，马海霞，荣辉. 金枪鱼中生物胺的防控技术与检测技术的研究发展. 食品与发酵工业. 2019(24): 262-268 .