Comparison of <i>in Vitro</i> Antioxidant, Probiotic and Anticancer Activities of Different Coarse Cereal Polysaccharides

YANG Sihui; MA Mingfang; CAO Ya'nan; REN Yuanhang; WAN Yan; ZOU Liang; PENG Lianxin

doi:10.13386/j.issn1002-0306.2022070332

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 1-10. > DOI: 10.13386/j.issn1002-0306.2022070332

YANG Sihui, MA Mingfang, CAO Ya'nan, et al. Comparison of in Vitro Antioxidant, Probiotic and Anticancer Activities of Different Coarse Cereal Polysaccharides[J]. Science and Technology of Food Industry, 2023, 44(1): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070332.

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Comparison of in Vitro Antioxidant, Probiotic and Anticancer Activities of Different Coarse Cereal Polysaccharides

YANG Sihui^{1, 2,},
MA Mingfang³,
CAO Ya'nan^{1, 2},
REN Yuanhang^{1, 2},
WAN Yan¹,
ZOU Liang^{1, 2},
PENG Lianxin^{1, 2, ,}

1.
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, China
2.
Sichuan Province Engineering Technology Research Center of Coarse Cereal Industralization, Chengdu 610106, China
3.
Qinghai Product Quality Inspection and Testing Institute, Xining 810004, China

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Received Date: August 01, 2022
Available Online: November 02, 2022

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Abstract

Abstract

Taking oat, adlay, quinoa, brown rice, yellow millet, barley, highland barley, tartary buckwheat, and rye as raw materials, and wheat as a control, 10 kinds of polysaccharides were obtained by water extraction and alcohol precipitation, respectively. The antioxidant activity of different polysaccharides, the inhibition of HCT116 activity of colon cancer cells and the effect on the growth of probiotics were determined and compared, and the polysaccharides with strong in vitro activity were screened out. In this study, DPPH, ABTS and hydroxyl radical assays were used to study the in vitro antioxidant activities of different polysaccharides, and MTT assay was used to detect the inhibitory effects of different polysaccharides on the proliferation of HCT116 cells at 48 and 72 h. Bifidobacterium longum Reuter, Bifidobacterium breve Reuter, Bifidobacterium adolescentis and Lactobacillus rhamnosus were cultured in vitro to study their effects on the growth of probiotics.The results showed that there were significant differences in the in vitro activities of different coarse cereal polysaccharides. Ten kinds of polysaccharides had scavenging abilities to DPPH, ABTS and hydroxyl radicals. Among them, tartary buckwheat polysaccharide had the strongest scavenging ability to three free radicals, which were 12.76 μmol Trolox/g DW, 39.56 μmol Trolox/g DW and 1615.32 μmol V_C/g DW, respectively. In terms of the effect on the growth of probiotics, 1% concentration of yellow millet, barley and oat polysaccharides could be more easily selected and utilized by Bifidobacterium breve Reuter, Bifidobacterium adolescentis and Lactobacillus rhamnosus. At 48 h, the inhibitory activity of polysaccharide on HCT116 cell proliferation was as follows: Wheat>brown rice>adlay>yellow millet; at 72 h, the inhibitory activity of polysaccharide on HCT116 cell proliferation was as follows: Wheat>adlay>brown rice>yellow millet>barley. The experimental results would provide reference for the quality evaluation of miscellaneous coarse cereals, and provide the basis for the selection of raw materials in the development of functional food.
- coarse cereal polysaccharides,
- antioxidation,
- probiotics,
- against colon cancer cells

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2.	蔡全龙，程昊，唐婷范，张文康，卫政涛，李利军. 双极性MgO纳米棒的制备、表征及其在原糖回溶糖浆脱色的应用. 食品研究与开发. 2025(03): 125-131 .
3.	干莉娜，张文康，赵家欣，唐婷范，卫政涛，程昊. 壳聚糖-甘蔗渣活性炭复合材料的制备及在糖汁清净中的应用. 中国调味品. 2024(04): 25-31 .
4.	林华，唐婷范，陈金足，苏萍萍，程昊. 水热法制备多孔硅酸镁及其对糖汁的脱色性能分析. 广西糖业. 2024(06): 419-425 .
5.	唐婷范，任逸，朱家庆，黄芳丽，程昊. 氢氧化镁对蔗糖溶液中单宁酸的吸附性能研究. 中国调味品. 2022(08): 46-50 .
6.	田鑫莉，张文康，李利军，卫政涛，程昊 . 硅酸镁-石灰乳法对原糖回溶糖浆脱色性能的研究. 食品工业. 2022(09): 51-55 .
7.	王琪浩，王余莲，王楠，李闯，张俊，朱益斌，刘珈伊，时天骄，林永瑾，田伊笛，苏德生，袁志刚. 高比表面积羟基硅酸镁的制备及其形成机理研究. 沈阳理工大学学报. 2022(06): 58-65 .