CHANG Xiangxiang, TIAN Yongli, YAN Juan, et al. Isolation, Purification and Characterization of β-Glucosidase from Cassava Roots[J]. Science and Technology of Food Industry, 2023, 44(3): 141−147. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050202.
Citation: CHANG Xiangxiang, TIAN Yongli, YAN Juan, et al. Isolation, Purification and Characterization of β-Glucosidase from Cassava Roots[J]. Science and Technology of Food Industry, 2023, 44(3): 141−147. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050202.

Isolation, Purification and Characterization of β-Glucosidase from Cassava Roots

  • The isolation, purification and enzymatic properties of β-glucosidase from cassava roots were studied. The crude enzyme extract was obtained from cassava roots with buffer solution, and the activity of crude enzyme was 9.37 U/g cassava dry weight. Purified by acetone precipitation, ion exchange chromatography and gel filtration chromatography, β-glucosidase activity was 1.14 U/g cassava dry weight, purified β-glucosidase purity increased by 14.62 times, the total activity recovery was 12.14%, the molecular weight of β-glucosidase was about 70 kDa. The Km and Vmax of the enzyme were 3.60 mmol/L and 12.36 µmol/(min·mg protein) respectively. The optimum pH was 7.0, and it was stable when the pH was between 6.0 and 8.0. It had good stability within 40 ℃, and 81.78% enzyme activity remained after 30 days at 4 ℃. Mn2+ and K+ promoted the enzyme to a certain extent. Al3+, Cu2+, Mg2+, Zn2+, Ca2+, Ba2+, Na+, urea and SDS had no significant effect on the enzyme activity (P>0.05). Fe3+, Fe2+, Ag+ and EDTA all inhibited the enzyme activity to varying degrees, among which Ag+ had the strongest inhibitory effect. The results can provide theoretical basis for the application of β-glucosidase in cassava roots in the future.
  • loading

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return