WANG Jiaoling, LI Jiaxing, YUE Yuanyuan. Process Optimization on Mixed Fermentation of Pickled Radish with Lactobacillus plantarum and Leuconostoc mesenteroides[J]. Science and Technology of Food Industry, 2023, 44(3): 172−182. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040282.
Citation: WANG Jiaoling, LI Jiaxing, YUE Yuanyuan. Process Optimization on Mixed Fermentation of Pickled Radish with Lactobacillus plantarum and Leuconostoc mesenteroides[J]. Science and Technology of Food Industry, 2023, 44(3): 172−182. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040282.

Process Optimization on Mixed Fermentation of Pickled Radish with Lactobacillus plantarum and Leuconostoc mesenteroides

More Information
  • Received Date: April 24, 2022
  • Available Online: December 01, 2022
  • In order to improve the quality of pickled radish, Lactobacillus plantarum and Leuconostoc mesenteroides were mixedly used as fermentation strains. The nitrite content, pH value, total acid content, total sugar content, texture character-istics and sensory score of pickled radish during fermentation were used as evaluation index to study the effects of strain ratio, inoculation amount, salt type and addition amount, calcium chloride and sucrose addition amount on the quality of pickled radish, and the contrastive analysis of taste was used by electronic tongue. Through the response surface test, the optimum fermentation parameters of pickled radish was as follows: The ratio of Lactobacillus plantarum and Leuconostoc mesenterica was 1.6:1, the inoculation amount was 5.7%, the addition amount of low sodium salt was 4.5%, the addition amount of calcium chloride was 0.3%, and the addition amount of sucrose was 2%. Under these conditions, compared with the natural fermentation group, the total acid content, brittleness and sensory score of inoculated radish after 6 days of fermentation increased significantly, which were 0.59 g/100 g, 108.8 N and 38.9 points, respectively, and the nitrite content was 0.41 mg/kg. The results of electronic tongue test showed that the mixed fermentation could effectively reduce the bitter-ness and aftertaste of pickled radish and enhance the acidity, sweetness and freshness. In conclusion, this study showed that mixed fermentation was beneficial to improve the texture, flavor and safety of pickled radish.
  • [1]
    杜伟利. 中国萝卜的栽培利用史研究[D]. 南京: 南京农业大学, 2019.

    DU W L. Research on the cultivation and utilization history of Chinese radish[D]. Nanjing: Nanjing Agricultural University, 2019.
    [2]
    戴希尧. 萝卜主要营养品质性状遗传的研究[D]. 北京: 中国农业科学院, 2012.

    DAI X Y. Genetic analysis of nutrient content in radish (Raphanus sativus L.)[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012.
    [3]
    GOYENECHE R, SCALA K D, ROURA S. Biochemical characterization and thermal inactivation of polyphenol oxidase from radish (Raphanus sativus var.sativus)[J]. LWT-Food Science and Technology,2013,54(1):57−62. doi: 10.1016/j.lwt.2013.04.014
    [4]
    梅明鑫, 刘卫, 宋颖, 等. 不同包装低盐腌制白萝卜贮藏货架期预测模型[J]. 食品与发酵工业,2017,43(4):69−77. [MEI M X, LIU W, SONG Y, et al. Storage shelf life prediction model of low salt pickled white radish by different packaging[J]. Food and Fermentation Industries,2017,43(4):69−77. doi: 10.13995/j.cnki.11-1802/ts.201704011
    [5]
    TAEKYUN S, MEEJUNG A, GI O K, et al. Biological activity of various radish species[J]. Oriental Pharmacy and Experimental Medicine,2015,15(2):105−111. doi: 10.1007/s13596-015-0183-9
    [6]
    CASTRO-TORRES I G, O-ARCINIEGA M, GALLEGOS-ESTUDILLO J, et al. Raphanus sativus L. var niger as a source of phytochemicals for the prevention of cholesterol gallstones[J]. Phytotherapy research: PTR,2014,28(2):167−71. doi: 10.1002/ptr.4964
    [7]
    TANG Y Y, ZHOU X R, HUANG S L, et al. Microbial community analysis of different qualities of pickled radishes by Illumina MiSeq sequencing[J]. Journal of Food Safety,2019,39(2):1−8.
    [8]
    周强, 刘蒙佳, 游慧英. 发酵条件对腌制萝卜品质影响及其工艺优化研究[J]. 中国调味品,2018,43(2):12−16. [ZHOU Q, LIU M J, YOU H Y. Research on the influences of fermentation conditions on the pickled radishes and the optimization of producing process[J]. China Condiment,2018,43(2):12−16. doi: 10.3969/j.issn.1000-9973.2018.02.004
    [9]
    JING P, SONG L H, SHEN S Q, et al. Characterization of phytochemicals and antioxidant activities of red radish brines during lactic acid fermentation[J]. Molecules,2014,19(7):9675−9688. doi: 10.3390/molecules19079675
    [10]
    李共国, 孙志栋. 乳酸菌影响腌制芥菜亚硝酸盐含量的通径分析[J]. 食品安全质量检测学报,2021,12(6):2351−2356. [LI G G, SUN Z D. Path analysis of lactic acid bacteria affecting nitrite content in pickled mustard[J]. Journal of Food Safety and Quality,2021,12(6):2351−2356. doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.06.045
    [11]
    刘永逸, 林华, 杨超, 等. 不同发酵方式对酸豆角品质和风味的影响[J]. 食品工业科技, 2022, 43(14): 43−51.

    LIU Y Y, LIN H, YANG C, et al. Effects of different fermentation methods on the quality and flavor of capers[J]. Science and Technology of Food Industry, 2022, 43(14): 43−51.
    [12]
    云琳, 毛丙永, 崔树茂, 等. 不同发酵方式对萝卜泡菜理化特性和风味的影响[J]. 食品与发酵工业,2020,46(13):69−75. [YUN L, MAO B Y, CUI S M, et al. Effects of different fermentation methods on the physicochemical properties and flavor of pickles[J]. Food and Fermentation Industries,2020,46(13):69−75. doi: 10.13995/j.cnki.11-1802/ts.023677
    [13]
    YE H Q, LANG X S, JI Y Y, et al. The interaction between Lactobacillus plantarum SC-5 and its biogenic amine formation with different salt concentrations in Chinese Dongbei Suancai[J]. Food Research International,2021,150(PB):110813.
    [14]
    PARK D W, KIM S H, PARK J H. Distribution and characterization of prophages in Lactobacillus plantarum derived from Kimchi[J]. Food Microbiology,2022,102:103913. doi: 10.1016/j.fm.2021.103913
    [15]
    李庆羊, 吴祖芳, 翁佩芳, 等. 植物乳杆菌和棒状乳杆菌对发酵萝卜干风味品质的影响[J]. 中国食品学报,2021,21(9):150−159. [LI Q Y, WU Z F, WENG P F, et al. Effects of Lactobacillus plantarum and Lactobacillus rodarum on flavor quality of fermented dried radish[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(9):150−159. doi: 10.16429/j.1009-7848.2021.09.016
    [16]
    史婷, 高甜甜, 刘伟, 等. 不同发酵剂对剁辣椒品质的影响[J]. 食品与发酵工业, 2022, 48(15): 144−153.

    SHI T, GAO T T, LIU W, et al. Effect of different starters on quality of chopped pepper[J]. 食品与发酵工业, 2022, 48(15): 144−153.
    [17]
    ZHANG P, ZHANG P F, WU J R, et al. Effects of Leuconostoc mesenteroides on physicochemical and microbial succession characterization of soybean paste, Da-jiang[J]. Lebensmittel Wissenschaft UND Technologie,2019,115(C):108028−108028.
    [18]
    PARK S E, SEO S H, KIM E J, et al. Changes of microbial community and metabolite in kimchi inoculated with different microbial community starters[J]. Food Chemistry,2018,274:558−565.
    [19]
    侯晓艳. 接种不同乳酸菌发酵对泡萝卜品质的影响[D]. 雅安: 四川农业大学, 2015.

    HOU X Y. Effects of different fermentation methods on the quality of pickled radishes[D]. Yaan: Sichuan Agricultural University, 2015.
    [20]
    李苹苹, 张冬梅, 全永亮, 等. 泡菜优良发酵剂的筛选研究[J]. 安徽农业科学,2012,40(16):8969−8971. [LI P P, ZHANG D M, QUAN Y L, et al. Selection of fermentation agent for pickles[J]. Journal of Anhui Agricultural Sciences,2012,40(16):8969−8971. doi: 10.3969/j.issn.0517-6611.2012.16.071
    [21]
    赵楠. 四川泡菜的主要特性及其成因分析[D]. 无锡: 江南大学, 2017.

    ZHAO N. Analysis of the main characteristics of Sichuan Paocai and its formation mechanism[D]. Wuxi: Jiangnan University, 2017.
    [22]
    孙钟雷, 熊玥, 李宇. 胭脂萝卜质地特性分析研究[J]. 中国调味品,2020,45(7):53−58. [SUN Z L, XIONG Y, LI Y. Study on the texture properties of carmine radish[J]. China Condiment,2020,45(7):53−58. doi: 10.3969/j.issn.1000-9973.2020.07.013
    [23]
    蔡乔宇. 黄酒酿造用米专用化评价体系构建[D]. 武汉: 武汉轻工大学, 2020.

    CAI Q Y. Construction of rice-specific evaluation system for brewing Huangjiu[D]. Wuhan: Wuhan Polytechnic University, 2020.
    [24]
    云琳. 不同发酵方式的萝卜泡菜风味特征解析及发酵剂菌种的筛选[D]. 无锡: 江南大学, 2020.

    YUN L. Analysis of the flavor characteristics of radish pickles with different fermentation methods and selection of the starter cultures[D]. Wuxi: Jiangnan University, 2020.
    [25]
    经骐源, 李婷, 曾凡坤, 等. 发酵剂对泡萝卜品质的影响[J]. 食品科学,2021,42(22):171−177. [JING Q Y, LI T, ZENG F K, et al. Effect of starters on the quality of pickled radish[J]. Food Science,2021,42(22):171−177. doi: 10.7506/spkx1002-6630-20201127-279
    [26]
    张锡茹, 关慧, 邢少华, 等. 泡菜微生物演替与风味物质变化的研究进展[J]. 食品科学,2021,42(23):294−305. [ZHANG X R, GUAN H, XING S H, et al. Advances in research on microbial succession and flavor changes in pickles[J]. Food Science,2021,42(23):294−305. doi: 10.7506/spkx1002-6630-20201213-143
    [27]
    王冉. 发酵方式对萝卜泡菜发酵过程中品质的影响[D]. 雅安: 四川农业大学, 2014.

    WANG R. Effects of fermentation on methods on the quality of pickled radishes in the fermentation[D]. Yaan: Sichuan Agricultural University, 2014.
    [28]
    朴泓洁, 黄存辉, 金清. 肠膜明串珠菌发酵对四川泡菜品质的影响[J]. 食品科技,2018,43(8):31−35. [PU H J, HUANG C H, JIN Q. Effects of Leuconostoc mesenteroides fermentation on quality of Sichuan pickle[J]. Food Science and Technology,2018,43(8):31−35. doi: 10.13684/j.cnki.spkj.2018.08.006
    [29]
    刘宗敏, 谭兴和, 周红丽, 等. 不同乳酸菌发酵萝卜干挥发性成分分析[J]. 食品科学,2017,38(24):144−149. [LIU Z M, TAN X H, ZHOU H L, et al. Analysis of volatile components in dried radish fermented by different Lactobacillus species[J]. Food Science,2017,38(24):144−149. doi: 10.7506/spkx1002-6630-201724023
    [30]
    洪冰. 大头菜发酵工艺及其品质变化研究[D]. 重庆: 西南大学, 2016.

    HONG B. Study on the fermention process and quality change of kohlrabi[D]. Chongqing: Southwest University, 2016.
    [31]
    王向阳, 吴婷, 俞兴伟. 食品添加剂对贮藏泡菜质地的影响[J]. 中国调味品,2015,40(12):73−75. [WANG X Y, WU T, YU X W. Effect of food additives on texture of stored pickles[J]. China Condiment,2015,40(12):73−75. doi: 10.3969/j.issn.1000-9973.2015.12.017
    [32]
    梁曹雯, 李清明, 邓洁红, 等. 泡萝卜感官评价及质构相关性分析[J]. 湖南农业科学,2016(8):99−102. [LIANG C W, LI Q M, DENG J H, et al. Correlation between texture and sensory evaluation of pickled radish[J]. Hunan Agricultural Sciences,2016(8):99−102. doi: 10.16498/j.cnki.hnnykx.2016.08.029
    [33]
    闫凯. 泡菜发酵工艺及保藏性的研究[D]. 武汉: 华中农业大学, 2013.

    YAN K. Study on salted technics and storing technology of pickles[D]. Wuhan: Huazhong Agricultural University, 2013.
    [34]
    刘超瑞. 几种食盐腌制甘蓝过程中亚硝酸盐变化规律[J]. 农产品加工(学刊),2012(5):49−51. [LIU C R. Several kinds of salted cabbage salt process change law of nitrate in central asia[J]. Academic Periodical of Farm Products Processing,2012(5):49−51.
    [35]
    王晶晶, 王蓉蓉, 刘成国, 等. 低钠盐发酵剁辣椒的挥发性成分研究[J]. 农产品加工,2017(18):32−36. [WANG J J, WANG R R, LIU C G, et al. Volatile compounds of chopped pepper with low sodium salt[J]. Farm Products Processing,2017(18):32−36. doi: 10.16693/j.cnki.1671-9646(X).2017.09.036
    [36]
    梅明鑫, 刘卫, 晏敏, 等. 萝卜低盐腌制过程中脆度变化原因探究[J]. 中国酿造,2017,36(12):27−33. [MEI M X, LIU W, YAN M, et al. Reasons for brittleness changes of radish during pickling process with low-salt content[J]. China Brewing,2017,36(12):27−33.
    [37]
    王馨. 用于发酵工程的乳酸菌筛选及复配菌剂研究[D]. 哈尔滨工业大学, 2021.

    WANG X. Screening of lactic acid bacteria used in fermentation engineering and engineering and research on compound bacteria[D]. Harbin: Harbin Institute of Technology, 2021.
    [38]
    陈小宇, 李丹, 叶峻. 腌制萝卜脆度的影响因素探究[J]. 农村科学实验,2020(2):28−31. [CHEN X Y, LI D, YE J. Factors affecting the crispness of radish[J]. Rural Scientific Experiment,2020(2):28−31.
  • Cited by

    Periodical cited type(1)

    1. 朱蕾,刘静,徐欢,潘迎捷,赵勇,张昭寰. 植物基食品安全风险及应对策略. 食品科学. 2024(20): 376-386 .

    Other cited types(1)

Catalog

    Article Metrics

    Article views (219) PDF downloads (30) Cited by(2)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return