Optimization of PEG 200 Extraction Process and <i>in Vitro</i> Antioxidant Activity of Polyphenols from <i>Dendrobium nobilis</i> Flower

ZHAO Yangyang; XING Jie; LI Li; ZHAN Maoling; LI Fei; LIN Lifangzhou; WANG Lan; YANG Wenyu

doi:10.13386/j.issn1002-0306.2022040072

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 260-267. > DOI: 10.13386/j.issn1002-0306.2022040072

ZHAO Yangyang, XING Jie, LI Li, et al. Optimization of PEG 200 Extraction Process and in Vitro Antioxidant Activity of Polyphenols from Dendrobium nobilis Flower[J]. Science and Technology of Food Industry, 2023, 44(1): 260−267. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040072.

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Optimization of PEG 200 Extraction Process and in Vitro Antioxidant Activity of Polyphenols from Dendrobium nobilis Flower

1.
College of Food and Biological Engineering, Xihua University, Chengdu 610039, China
2.
Guizhou Xiancao Biological Technology Co., Ltd., Chishui 564700, China

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Received Date: April 07, 2022
Available Online: October 25, 2022

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Abstract

Abstract

Objective: To extract polyphenols in Dendrobium nobilis flower (DNF) through a green process method and to evaluate its antioxidant activity in vitro. Methods: Using PEG 200 as solvent, the single factors tests and response surface experiments were conducted to optimize the extraction conditions on the yield of polyphenols from DNF. AB-8 macroporous resin adsorption technology was applied for the enrichment of the above polyphenols and the recycling of PEG 200. The ABTS and DPPH assays were performed to investigate the antioxidant properties of those polyphenols. Results: The optimal parameters were as follows: The solvent volume fraction was 55%, the liquid-solid ratio was 38:1 mL·g⁻¹, the ultrasonic time was 99 min, the ultrasonic temperature was 80 ℃. The polyphenols yield could reached up to 338.88 mg·g⁻¹ in these conditions. Within the test concentration range, the antioxidant capacity in vitro of those polyphenols was similar to that of ascorbic acid. The IC₅₀ of ABTS and IC₅₀ of DPPH free radicals of the polyphenols were 4.486 and 2.792 mg·mL⁻¹ respectively. Conclusion: The green solvent PEG 200 can be used for the extraction of polyphenols from Dendrobium nobilis flower and can be reused; the in vitro antioxidant capacity of the extracted polyphenols is strong, which has good prospects for development and application.
- Dendrobium nobilis flower,
- green extraction,
- polyphenols,
- ultrasonic assisted extraction,
- antioxidant activity

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[1]	SHU Y, GONG Q H, WU Q, et al. Alkaloids enriched extract from Dendrobium nobile Lindl. attenuates tau protein hyperphosphorylation and apoptosis induced by lipopolysaccharide in rat brain[J]. Phytomedicine International Journal of Phytotherapy & Phytopharmacology,2014,21(5):712−716.
[2]	ZHANG Y, ZHANG L H, LIU J J, et al. Dendrobium officinale leaves as a new antioxidant source[J]. Journal of Functional Foods,2017,37:400−415. doi: 10.1016/j.jff.2017.08.006
[3]	郑家欢, 吴观健, 吴岳滨, 等. GC-MS分析金钗石斛花挥发油成分[J]. 中药材,2016,39(8):1797−1799. [ZHENG J H, WU G J, WU Y B, et al. GC-MS analysis of volatile oil components in Dendrobium nobile flowers[J]. Journal of Chinese Medicinal Materials,2016,39(8):1797−1799.
[4]	宋小蒙. 金钗石斛花花色苷和精油的纯化、产物分析及其活性研究[D]. 无锡: 江南大学, 2017. SONG X M. Study on the purification, components analysis and biological activity of anthocyanin and volatile oil from Dendrobium nobile Lindl. flower[D]. Wuxi: Jiangnan University, 2017.
[5]	KAROLINA J, AGNIESZKA Ł, IZABELA G, et al. Edible flowers extracts as a source of bioactive compounds with antioxidant properties-in vitro studies[J]. Applied Sciences,2021,11(5):2120. doi: 10.3390/app11052120
[6]	PIRES T C S P, DIAS M I, BARROS L, et al. Edible flowers as sources of phenolic compounds with bioactive potential[J]. Food Research International,2018,105:580−588. doi: 10.1016/j.foodres.2017.11.014
[7]	DIMITRIOS S. Antioxidant activity of polyphenolic plant extracts[J]. Antioxidants,2019,9(1):19. doi: 10.3390/antiox9010019
[8]	GKE P Y, SUSAN B, PASCAL D. Antibacterial activity of polyphenol-rich pomegranate peel extract against Cronobacter sakazakii[J]. International Journal of Food Properties,2019,22(1):985−993. doi: 10.1080/10942912.2019.1622564
[9]	PRITAM B B, SANG E H, PREETHI V, et al. Functions of polyphenols and its anticancer properties in biomedical research: A narrative review[J]. Translational Cancer Research,2020,9(12):7619−7631. doi: 10.21037/tcr-20-2359
[10]	OLUWASEUN R, NOUR H A, CHINONSO I U. Extraction of phenolic compounds: A review[J]. Current Research in Food Science,2021,4:200−214. doi: 10.1016/j.crfs.2021.03.011
[11]	MAI X M, LIU Y T, TANG X, et al. Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled totemperature induced cloud point extraction[J]. Ultrasonics Sonochemistry,2020,66:105073. doi: 10.1016/j.ultsonch.2020.105073
[12]	HUANG H, XU Q, TARUN B, et al. Ultrasonic impact on viscosity and extraction efficiency of polyethylene glycol: A greener approach for anthocyanins recovery from purple sweet potato[J]. Food Chemistry,2019,283:59−67. doi: 10.1016/j.foodchem.2019.01.017
[13]	ZHOU T, XIAO X H, LI G K, et al. Study of polyethylene glycol as a green solvent in the microwave-assisted extraction of flavone and coumarin compounds from medicinal plants[J]. Journal of Chromatography A,2011,1218(23):3608−3615. doi: 10.1016/j.chroma.2011.04.031
[14]	唐静月, 颜美秋, 齐芳芳, 等. 铁皮石斛花总黄酮提取工艺优化及体外抗氧化活性研究[J]. 浙江中医药大学学报,2017,41(3):235−242. [TANG J Y, YAN M Q, QI F F, et al. Study on optimum extraction of total flavones in Dendrobium officinale flower and its antioxidant activity in vitro[J]. Journal of Zhejiang Chinese Medical University,2017,41(3):235−242. doi: 10.16466/j.issn1005-5509.2017.03.017
[15]	李娟, 麻晓雪, 李顺祥, 等. 铁皮石斛中总酚的含量测定[J]. 中国实验方剂学杂志,2013,19(24):60−62. [LI J, MA X X, LI S X, et al. Determination of total phenols in Dendrobii officinalis Caulis J]. Chinese Journal of Experimental Traditional Medical Formulae,2013,19(24):60−62.
[16]	LIU H, DU X L, YUAN Q P, et al. Optimisation of enzyme assisted extraction of silybin from the seeds of Silybum marianum by Box-Behnken experimental design[J]. Phytochemical Analysis,2010,20(6):475−483.
[17]	ZHAO C F, LI S, LI S J, et al. Extraction optimization approach to improve accessibility of functional fraction based on combination of total polyphenol, chromatographic profiling and antioxidant activity evaluation: Pyracantha fortuneana fruit as an example[J]. Journal of Functional Foods,2013,5(2):715−728. doi: 10.1016/j.jff.2013.01.017
[18]	JU J Q, YU Y L, LIU Y L, et al. Physical properties and interaction studies for polyethylene glycol 200 and 1, 2-propanediamine system at temperature from 293.15 to 318.15 K under atmospheric pressure[J]. Journal of Molecular Liquids,2022,350:118419. doi: 10.1016/j.molliq.2021.118419
[19]	JAVIER A D, MOSHE R, CARLOS A C. Extraction of phenolic compounds from spent blackberry pulp by enhanced-fluidity liquid extraction[J]. AIChE Journal,2019,65(8):e16609.
[20]	陈思卿, 胡齐, 潘飞兵, 等. 超声辅助提取槟榔核多酚工艺优化及降糖活性研究[J]. 中国林副特产,2022,1:8−11. [CHEN S Q, HU Q, PAN F B, et al. Optimization and hypoglycemic activity of ultrasound-assisted extraction of polyphenols from betel-nut[J]. Forest By-Product and Speciality in China,2022,1:8−11.
[21]	张珍林, 闵运江, 黄仁术, 等. 霍山石斛和铁皮石斛干花成分含量和抗氧化性的比较[J]. 天然产物研究与开发,2020,32(7):1104−1110. [ZHANG Z L, MIN Y J, HUANG R S, et al. Comparison of dried Dendrobium huoshanense and D. officinale in component content and antioxidant activity[J]. Natural Product Research and Development,2020,32(7):1104−1110.
[22]	耿文慧, 卢绍基, 明艳林. 铁皮石斛化学成分及其抗氧化活性研究[J]. 亚热带植物科学,2020,49(6):438−442. [GENG W H, LU S J, MING Y L. The chemical constituents and antioxidant activities of Dendrobium officinale[J]. Subtropical Plant Science,2020,49(6):438−442. doi: 10.3969/j.issn.1009-7791.2020.06.005
[23]	ŠURAN J, CEPANEC I, MAŠEK T, et al. Nonaqueous polyethylene glycol as a safer alternative to ethanolic propolis extracts with comparable antioxidant and antimicrobial activity[J]. Antioxidants,2021,10(6):978. doi: 10.3390/antiox10060978
[24]	CARLOS-EDUARDO N-C, MARY-LUCÍA I-C, ANDRÉS-MAURICIO H-B, et al. The phenolic compounds, tocopherols, and phytosterols in the edible oil of guava (Psidium guava) seeds obtained by supercritical CO₂ extraction[J]. Journal of Food Composition and Analysis,2020,89:103467. doi: 10.1016/j.jfca.2020.103467
[25]	SEYED A K, SAMIN H, MOHAMMAD R S S. Current trends in sample preparation by solid-phase extraction techniques for the determination of antibiotic residues in foodstuffs: A review[J]. Critical Reviews in Food Science and Nutrition,2021,61(20):3361−3382. doi: 10.1080/10408398.2020.1798349
[26]	JINZE D, JARI H, TAPANI V, et al. Chromatographic recovery and purification of natural phytochemicals from underappreciated willow bark water extracts[J]. Separation and Purification Technology,2020,261:118247.
[27]	CAI X Y, YU W, C Y, et al. Green extraction of cannabidiol from industrial hemp (Cannabis sativa L.) using deep eutectic solvents coupled with further enrichment and recovery by macroporous resin[J]. Journal of Molecular Liquids,2019:287.
[28]	LIU R L, YU P, GE X L, et al. Establishment of an aqueous PEG200-based deep eutectic solvent extraction and enrichment method for pumpkin (Cucurbita moschata) seed protein[J]. Food Analytical Methods,2017,10(6):1669−1680. doi: 10.1007/s12161-016-0732-y
[29]	ZHOU X Y, LIU R L, MA X, et al. Polyethylene glycol as a novel solvent for extraction of crude polysaccharides from pericarpium granati[J]. Carbohydrate Polymers,2014,101(1):886−889.
[30]	ZHOU X Y, ZHANG J, XU R P, et al. Aqueous biphasic system based on low-molecular-weight polyethylene glycol for one-step separation of crude polysaccharides from Pericarpium granati using high-speed countercurrent chromatography[J]. Journal of Chromatography A,2014,1362:129−134. doi: 10.1016/j.chroma.2014.08.034
[31]	LIAN P, LI L, QI Q, et al. Extraction and determination of flavonoid in an aqueous two-phase system of PEG600-Triton X-100 combined surfactant[J]. Analytical Laboratory,2005,24(5):55−58.
[32]	LIU L, LIU R L, ZHANG J, et al. Study on the PEG-based microwave-assisted extraction of flavonoid compounds from persimmon leaves[J]. Journal of Separation Science,2012,35(23):3412−3420. doi: 10.1002/jssc.201200495

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