Screening of A Neutral Protease-producing Strain and Optimization of Fermentation Conditions

LI Na; FU Junjie; LIU Jun; WEN Xueping; LI Li

doi:10.13386/j.issn1002-0306.2022040055

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 189-199. > DOI: 10.13386/j.issn1002-0306.2022040055

LI Na, FU Junjie, LIU Jun, et al. Screening of A Neutral Protease-producing Strain and Optimization of Fermentation Conditions[J]. Science and Technology of Food Industry, 2023, 44(1): 189−199. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040055.

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Screening of A Neutral Protease-producing Strain and Optimization of Fermentation Conditions

LI Na^1,,
FU Junjie¹,
LIU Jun¹,
WEN Xueping¹,
LI Li^{1, 2, ,}

1.
College of Bioengineering, Sichuan University of Science & Engineering, Yibin 644000, China
2.
Key Laboratory of Solid-state Fermentation Resource Utilization in Sichuan Province, Yibin 644000, China

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Received Date: April 07, 2022
Available Online: November 05, 2022

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Abstract

Abstract

Currently, low enzyme activity and high raw material cost seriously restricted the development of the industrial production of protease. In this study, transparent circle and shaking flask fermentation methods were used to screen out a neutral protease-producing strain, which was identified as Bacillus velezensis by morphological observation, physiological and biochemical results and 16S rDNA analysis, and named ppr3. The medium composition and fermentation conditions for this strain to produce neutral protease were optimized by one-at-a-time strategy and response surface methodology. The results showed that: The fermentation temperature was 33 ℃, inoculation amount was 8.5% (v/v), initial pH was 5.7, lactose 5.55 g/L, rapeseed 31.89 g/L, yeast extract 7.09 g/L, tween-80 0.03%, MgSO₄ 0.04%, K₂HPO₄ 0.04%, the protease activity was 500 U/mL. Compared with before optimization, it increased by 132%. The addition of rapeseed meal as nitrogen source to the medium significantly reduced the fermentation cost and provided a new way for the reuse of agricultural and sideline products, which had major implications in protease industrial fermentation.
- Bacillus velezensis,
- screening,
- protease activity,
- optimization of fermentation conditions

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References

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