QU Guangfan, WANG Rui, MA Chao, et al. Isolation and Identification of the Pathogens of 'East Red' Kiwifruit Soft Rot and Its Control Effect by Melatonin[J]. Science and Technology of Food Industry, 2023, 44(2): 170−177. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030364.
Citation: QU Guangfan, WANG Rui, MA Chao, et al. Isolation and Identification of the Pathogens of 'East Red' Kiwifruit Soft Rot and Its Control Effect by Melatonin[J]. Science and Technology of Food Industry, 2023, 44(2): 170−177. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030364.

Isolation and Identification of the Pathogens of 'East Red' Kiwifruit Soft Rot and Its Control Effect by Melatonin

More Information
  • Received Date: March 29, 2022
  • Available Online: November 11, 2022
  • To clarify the species of the pathogens of soft rot of 'East Red' kiwifruit and the effect of melatonin on its control, using naturally disease 'East Red' kiwifruit as test material, the pathogenic bacteria of soft rot were isolated and cultured using a tissue isolation method. The pathogenic bacteria isolated were tested for pathogenicity according to Koch's rule, and identified based on fungal morphological identification and rDNA-ITS sequence analysis. The effect of melatonin at different concentrations (0, 100, 300 and 500 μmol/L) on the control of the two pathogenic strains isolated was also investigated. The results showed that Diaporthe phaseolorum and Botryosphaeria dothidea were the main pathogenic bacteria causing soft rot of 'East Red' kiwifruit during storage. The in vitro inhibition test showed that melatonin significantly inhibited the mycelial growth of both pathogens (P<0.05). At 5 d of incubation, the inhibition rates of 500 μmol/L melatonin were 71.22% and 63.34% for Diaporthe phaseolorum and Botryosphaeria dothidea, respectively. At the meanwhile, melatonin was effective in controlling the expansion of soft rot lesions under damage inoculation. At 5 d of damage inoculation, the diameter of fruit spots after the inoculation of Diaporthe phaseolorum and Botryosphaeria dothidea with 500 μmol/L melatonin treatment was only 37.40% and 45.50% of the control group, respectively. It showed that melatonin had potential application value for the control of soft rot pathogens of 'East Red' kiwifruit.
  • [1]
    黄文俊, 刘小莉, 张琦, 等. 黄肉红心猕猴桃‘东红’果实在不同贮藏方式下的生理和品质变化研究[J]. 植物科学学报,2019,37(3):382−388. [HUANG W J, LIU X L, ZHANG Q, et al. Physiological and quality changes of yellow flesh red heart kiwifruit 'Donghong' under different storage methods[J]. Journal of Plant Science,2019,37(3):382−388.
    [2]
    HU H, L P, WANG Y, et al. Hydrogen-rich water delays postharvest ripening and senescence of kiwifruit[J]. Food Chem,2014,156:100−109. doi: 10.1016/j.foodchem.2014.01.067
    [3]
    ZHOU Y, GONG G S, CUI Y L, et al. Identification of botryosphaeriaceae species causing kiwifruit rot in Sichuan Province, China[J]. Plant Disease,2015,99(5):699−708. doi: 10.1094/PDIS-07-14-0727-RE
    [4]
    THOMIDIS T, EXADAKTYLOU E, CHEN S F. Diaporthe neotheicola, a new threat for kiwifruit in Greece[J]. Crop Protection,2013,47(5):35−40.
    [5]
    LI L, PAN H, CHEN M Y, et al. First report of Pestalotiopsis microspore causing postharvest rot of kiwifruit in Hubei Province, China[J]. Plant Disease,2016,100(10):21−61.
    [6]
    左盼盼, 付苏, 彭丽桃, 等. 猕猴桃采后软腐病病原菌鉴定及香芹酚对其控制效果[J]. 华中农业大学学报,2020,39(6):15−22. [ZUO P P, FU F, PENG L T, et al. Identification of pathogen of postharvest soft rot of kiwifruit and control effect of carvacrol[J]. Journal of Huazhong Agricultural University,2020,39(6):15−22.
    [7]
    王小洁, 李士谣, 李亚巍, 等. 猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J]. 植物保护学报,2017,44(5):826−832. [WANG X J, LI S Y, LI Y W, et al. Isolation and identification of pathogen of kiwifruit soft rot and screening of its control agents[J]. Journal of Plant Protection,2017,44(5):826−832.
    [8]
    吴文能, 张起, 雷霁卿, 等. “贵长”猕猴桃软腐病病原菌分离鉴定及抑菌药剂筛选[J]. 北方园艺,2018(16):47−54. [WU W N, ZHANG Q, LEI J Q, et al. Isolation and identification of pathogen of "Guichang" kiwifruit soft rot and screening of antimicrobial agents[J]. Northern Horticulture,2018(16):47−54.
    [9]
    REITER R J, TAN D X, ZHOU Z. Phytomelatonin: assisting plants to survive and thrive[J]. Molecules,2015,20(4):7396−7437. doi: 10.3390/molecules20047396
    [10]
    GAO H, LU Z M, YANG Y, et al. Melatonin treatment reduces chilling injury in peach fruit through its regulation of membrane fatty acid contents and phenolic metabolism[J]. Food Chemistry,2018,245:87−93.
    [11]
    WANG F, ZHANG X P, YANG Q Z, et al. Exogenous melatonin delays postharvest fruit senescence and maintains the quality of sweet cherries[J]. Food Chemistry,2019,301:1−10.
    [12]
    孙子荀, 倪照君, 高志红, 等. 外源褪黑素提高草莓黑斑病抗性的效果和作用机制初探[J]. 西北植物学报,2020,40(10):1679−1687. [SUN Z X, NI Z J, GAO Z H, et al. Preliminary study on the effect and mechanism of exogenous melatonin on increasing strawberry black spot disease resistance[J]. Northwest Botanical Journal,2020,40(10):1679−1687. doi: 10.7606/j.issn.1000-4025.2020.10.1679
    [13]
    曹晶晶, 于子超, 张颖, 等. 外源褪黑素对苹果采后灰霉病的防效及防御酶活性的影响[J]. 植物生理学报,2017,53(9):1753−1760. [CAO J J, YU Z C, ZHANG Y, et al. The effect of exogenous melatonin on the control effect of postharvest apple gray mold and the activity of defensive enzymes[J]. Acta Plant Physiology,2017,53(9):1753−1760.
    [14]
    ZHANG Z K, WANG T, LIU G S. Inhibition of downy blight and enhancement of resistance in litchi fruit by postharvest application of melatonin[J]. Food Chemistry,2021,347:2−8.
    [15]
    生吉萍, 赵瑞瑞, 陈玲玲, 等. 褪黑素采前喷施对采后番茄果实抗病性和贮藏品质的影响[J]. 食品科学,2020,41(9):188−193. [SHENG J P, ZHAO R R, CHEN L L, et al. Effect of spraying melatonin before harvest on disease resistance and storage quality of tomato fruits after harvest[J]. Food Science,2020,41(9):188−193.
    [16]
    向妙莲, 吴帆, 李树成, 等. 褪黑素处理对梨果实采后黑斑病及贮藏品质的影响[J]. 中国农业科学,2022,55(4):785−795. [XIANG M L, WU F, LI S C, et al. Effects of melatonin treatment on postharvest black spot and storage quality of pear fruit[J]. Chinese Agricultural Science,2022,55(4):785−795. doi: 10.3864/j.issn.0578-1752.2022.04.013
    [17]
    唐琦. 采后褪黑素处理对大枣青霉病和贮藏品质的影响[D]. 锦州: 渤海大学, 2019

    TANG Q. Effects of postharvest melatonin treatment on penicilliosis and storage quality of jujube [D]. Jinzhou: Bohai University, 2019.
    [18]
    王荣波, 陈姝樽, 李本金, 等. 福建省彩叶草叶斑病病原菌的分离鉴定与室内药剂筛选[J]. 福建农业学报,2021,36(12):1471−1477. [WANG R B, CHEN S Z, LI B J, et al. Isolation and identification of pathogen of leaf spot of color leafgrass in Fujian Province and screening of indoor fungicides[J]. Fujian Agricultural Journal,2021,36(12):1471−1477.
    [19]
    SUDHIR K, GLEN S, KOICHIRO T. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology & Evolution,2016,33(7):1870.
    [20]
    EDWARDS S G, SEDDON B. Mode of antagonism of Brevibacillus brevis against Botrytis cinerea in vitro[J]. Journal of Applied Microbiology,2010,91(4):652−659.
    [21]
    王丹, 张静, 贾晓曼, 等. 蓝莓采后主要病原菌的分离鉴定及肉桂精油抑菌效果[J]. 食品科学,2019,40(24):167−172. [WANG D, ZHANG J, JIA Z M, et al. Isolation and identification of main postharvest pathogens of blueberry and antibacterial effect of cinnamon essential oil[J]. Food Science,2019,40(24):167−172. doi: 10.7506/spkx1002-6630-20181029-338
    [22]
    石浩, 王仁才, 王芳芳, 等. 猕猴桃软腐病主要病原菌的分离、鉴定及其生长特性研究[J]. 核农学报,2020,34(11):2425−2434. [SHI H, WANG R C, WANG F F, et al. Isolation, identification and growth characteristics of main pathogens of kiwifruit soft rot[J]. Journal of Nuclear Agriculture,2020,34(11):2425−2434. doi: 10.11869/j.issn.100-8551.2020.11.2425
    [23]
    李爱华, 郭小成. 秦美猕猴桃软腐病的发生规律与防治初探[J]. 陕西农业科学,1994(3):44. [LI A H, GUO X C. Occurrence and control of soft rot of kiwifruit in Qinmei[J]. Shaanxi Agricultural Science,1994(3):44.
    [24]
    虞凡霜, 强遥, 秦双林, 等. 一株侵染黄桃果实的葡萄座腔菌菌株鉴定[J]. 江西农业大学学报,2021,43(3):566−573. [YU F S, QIANG Y, QING S L, et al. Identification of a strain of Botryosphaeria dothidea infecting yellow peach fruit[J]. Acta Agriculturae Universitatis Jiangxiensis,2021,43(3):566−573. doi: 10.13836/j.jjau.2021063
    [25]
    朱琪丽, 赵会长, 谢甲涛, 等. 葡萄座腔菌侵染柑橘果实的报道[J]. 植物病理学报,2018,48(2):154−158. [ZHU Q L, ZHAO H C, XIE J T, et al. A report on the infection of citrus fruit by Botryosphaeria dothidea[J]. Journal of Plant Pathology,2018,48(2):154−158. doi: 10.13926/j.cnki.apps.000126
    [26]
    熊桃. 柑橘间座壳菌的群体遗传结构和有性生殖研究[D]. 杭州: 浙江大学, 2021

    XIONG T. Study on population genetic structure and sexual reproduction of citrus Diaporthe phaseolorum[D]. Hangzhou: Zhejiang University, 2021.
    [27]
    雷霁卿, 吴文能, 刘颖, 等. 贵州六盘水地区“红阳”猕猴桃软腐病病原菌分离鉴定及致病力差异测定[J]. 北方园艺,2019(4):31−38. [LEI J Q, WU W N, LIU Y, et al. Isolation and identification of the pathogen of “Hongyang” kiwifruit soft rot in Liupanshui area of guizhou and determination of pathogenicity difference[J]. Northern Horticulture,2019(4):31−38.
    [28]
    HE J G, CHENG Y D, GUAN J F, et al. Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development[J]. Journal of Integrative Agriculture,2017,16(2):471−477. doi: 10.1016/S2095-3119(16)61496-X
    [29]
    LI S G, XU Y H, YANG B, et al. Melatonin treatment inhibits gray mold and induces disease resistance in cherry tomato fruit during postharvest[J]. Postharvest Biology and Technology,2019,157(C):110962.
    [30]
    ZHANG L L, YU Y W, CHANG L L, et al. Melatonin enhanced the disease resistance by regulating reactive oxygen species metabolism in postharvest jujube fruit[J]. Journal of Food Processing and Preservation,2022,182:111706.
    [31]
    唐琦. 采后褪黑素处理对大枣青霉病和贮藏品质的影响[D]. 锦州: 渤海大学, 2019

    TANG Q. Effects of melatonin treatment on penicilliosis and storage quality of jujube[D]. Jinzhou: Bohai Universityy, 2019.
    [32]
    王甜. 外源褪黑素对荔枝采后褐变和病害的控制作用[D]. 海口: 海南大学, 2020

    WANG T. Control effect of exogenous melatonin on postharvest browning and disease of litchi[D]. Haikou: Hainan University, 2020.
  • Cited by

    Periodical cited type(1)

    1. 杨丽娟,王伟伟,许勇泉,江和源. 外源酶在红茶加工中的应用研究进展. 食品工业科技. 2024(07): 344-351 . 本站查看

    Other cited types(2)

Catalog

    Article Metrics

    Article views (165) PDF downloads (9) Cited by(3)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return