Comparative Study on Hepatoprotective Effects of Alcohol Extract and Total Flavonoids of <i>Penthorum chinense</i> Pursh against Alcohol-induced Subacute Liver Injury in Mice

JIU Hong; FU Manling; LIU Hongsheng; ZHANG Shibo; HOU Xiaofang; YAO Jiqiang; YUAN Yefei

doi:10.13386/j.issn1002-0306.2022030254

Volume 44 Issue 3

Jan. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(3): 391-397. > DOI: 10.13386/j.issn1002-0306.2022030254

JIU Hong, FU Manling, LIU Hongsheng, et al. Comparative Study on Hepatoprotective Effects of Alcohol Extract and Total Flavonoids of Penthorum chinense Pursh against Alcohol-induced Subacute Liver Injury in Mice[J]. Science and Technology of Food Industry, 2023, 44(3): 391−397. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030254.

Citation:

PDF (2646 KB)

Comparative Study on Hepatoprotective Effects of Alcohol Extract and Total Flavonoids of Penthorum chinense Pursh against Alcohol-induced Subacute Liver Injury in Mice

1.
Pharmacy College, Southwest Medical University, Luzhou 646000, China
2.
Science and Technology Department, Southwest Medical University, Luzhou 646000, China
3.
Department of Pharmacy, Luzhou Hospital of Traditional Chinese Medicine, Luzhou 646000, China
4.
Luzhou Feixiang Pharmaceutical Technology Co., Ltd., Luzhou 646000, China

More Information

Received Date: March 21, 2022
Available Online: December 03, 2022

Graphical Abstract

Abstract

Abstract

Object: To compare the hepatoprotective effects of alcohol extract and total flavonoids of Penthorum chinense Pursh on alcohol-induced subacute liver injury in mice. Methods: The mice were randomized into the normal group, model group, positive group (bifendate, 150 mg/kg), ethanol extract of Penthorum chinense Pursh with three different dose treatment groups (400, 800 and 1600 mg/kg), and total flavonoids of Penthorum chinense Pursh with three different dose treatment groups (100, 200 and 400 mg/kg). The model of subacute alcoholic liver injury in mouse was set up by intragastric administration of alcohol. The liver index, pathological changes of liver tissue and serum biochemical indexes were measured in each group. Results: Compared with mice in the model group, the hepatic index was decreased significantly (P<0.05), the liver injury pathological state was ameliorated, the activities of serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and the content of total bilirubin (TBIL), total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) were decreased significantly (P<0.05, P<0.01) in the high and medium treatment groups of alcohol extract and total flavonoids of Penthorum chinense Pursh. The activities of serum ALT, AST, ALP, LDH as well as the content of TBIL, TC, TG and LDL-C in the total flavonoids group were significantly lower than those in the alcohol extract group with the same crude drug dose (P<0.05, P<0.01). Conclusion: Both alcohol extract and total flavonoids of Penthorum chinense Pursh can protect subacute alcoholic liver injury, but total flavonoids does better than alcohol extract. The total flavonoids is a main functional component of Penthorum chinese Pursh to protect alcoholic liver injury.

FullText(HTML)

References (30)

References

[1]	付双楠, 朱平生, 高达. 中医药防治化学性肝损伤的研究进展[J]. 中医学报,2017,32(3):449−454. [FU S N, ZHU P S, GAO D. Progress of TCM studies in TCM treatment of liver injury due to chemical factors[J]. Acta Chinese Medicine,2017,32(3):449−454. doi: 10.16368/j.issn.1674-8999.2017.03.116
[2]	刘袆帆, 朱珍, 苏敏璇, 等. 多糖对酒精性肝损伤干预作用的研究进展[J]. 现代食品科技,2020,36(8):379−393. [LIU H F, ZHU Z, SU M X, et al. Efficacy of polysaccharides as alcoholic liver disease therapeutics: A review[J]. Modern Food Science and Technology,2020,36(8):379−393. doi: 10.13982/j.mfst.1673-9078.2020.8.0185
[3]	SALEEM T S M, CHETTY C M, RAMKANTH S, et al. Hepatoprotective herbs-A review[J]. International Journal of Research in Pharmaceutical Sciences,2010,1(5):1−5.
[4]	唐川, 李志, 王升贵, 等. 霍山石斛多糖的制备、功能和产品开发的研究进展[J]. 食品工业科技,2019,40(3):313−320. [TANG C, LI Z, WANG S G, et al. Research progress on preparation, function and product development of polysaccharide of Dendrobium huoshanense[J]. Science and Technology of Food Industry,2019,40(3):313−320. doi: 10.13386/j.issn1002-0306.2019.03.050
[5]	李志满, 邵紫君, 李珊珊, 等. 人参枳子提取物对小鼠酒精性肝损伤的保护作用[J]. 食品工业科技,2019,40(14):302−306, 313. [LI Z M, SHAO Z J, LI S S, et al. Protective effect of extract from Panax ginsen and Hovenia dulcis Thunb against alcohol-induced liver injury in mice[J]. Science and Technology of Food Industry,2019,40(14):302−306, 313.
[6]	孟海涛, 汪鹤, 查学强, 等. 霍山石斛不同提取物抗小鼠亚急性酒精性肝损伤活性的比较研究[J]. 食品科学,2015,36(13):229−234. [MENG H T, WANG H, ZHA X Q, et al. Comparison of hepatoprotective effects of different extracts from Dendrobium huoshanense against alcohol-induced subacute liver injury in mice[J]. Food Science,2015,36(13):229−234. doi: 10.7506/spkx1002-6630-201513042
[7]	LOGGE W B, MORLEY K C, HABER P S, et al. Executive function moderates responses to appetitive cues: A study in severe alcohol use disorder and alcoholic liver disease[J]. Alcohol and Alcoholism,2019,54(1):38−46. doi: 10.1093/alcalc/agy083
[8]	刘畅, 成玉梁, 郭亚辉, 等. 芦荟多糖联合茶多酚对小鼠酒精性肝损伤的预防作用[J]. 食品工业科技,2019,40(22):300−305. [LIU C, CHENG Y L, GUO Y H, et al. Preventive effect of aloe polysaccharide combined with tea polyphenol on alcoholic liver injury in mice[J]. Science and Technology of Food Industry,2019,40(22):300−305. doi: 10.13386/j.issn1002-0306.2019.22.052
[9]	牛佳卉, 袁静, 张慧芳, 等. 王浆酸对小鼠酒精性肝损伤的保护作用[J]. 食品工业科技,2020,41(3):291−296. [LIU J H, YUAN J, ZHANG H F, et al. Protective effect of royal jelly acid on the alcoholliver injury in mice[J]. Science and Technology of Food Industry,2020,41(3):291−296. doi: 10.13386/j.issn1002-0306.2020.03.048
[10]	SHEN T, LIU Y, SHANG J, et al. Incidence and etiology of drug-induced liver injury in mainland China[J]. Gastroenterology,2019,156(8):1−12. doi: 10.1053/j.gastro.2019.01.006
[11]	白路平, 乔向宇, 吉日木图, 等. 骆驼乳对小鼠酒精性肝损伤的保护作用[J]. 中国食品学报,2022,22(2):140−149. [BAI L P, QIAO X Y, JIRIMUTU, et al. Protective effect of camel milk on alcoholic liver injury in mice[J]. Journal of Chinese Institute of Food Science and Technology,2022,22(2):140−149. doi: 10.16429/j.1009-7848.2022.02.016
[12]	LIU Y S, YUAN M H, ZHANG C Y, et al. Puerariae lobatae radix flavonoids and puerarin alleviatealcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism[J]. BiomedicineI & Pharmcotherapy,2021,134:111121−111128.
[13]	DING Q C, JIN Z, DONG J H, et al. Bioactivity evaluation of pinocembrin derivatives from Penthorum chinense Pursh stems[J]. Natural Product Communications,2019,14(9):1−9.
[14]	WANG A Q, LI M X, HUANG M, et al. A review of Penthorum chinense Pursh for hepatoprotection: Traditional use, phytochemistry, pharmacology, toxicology and clinical trials[J]. Journal of Ethnopharmacol,2020,251(6):26−32.
[15]	HUANG D D, JIANG Y, CHEN W S, et al. Polyphenols with anti-proliferative activities from Penthorum chinense Pursh[J]. Molecules,2014,19(8):11045−11055. doi: 10.3390/molecules190811045
[16]	张剑, 伍淑明, 杨肖, 等. 赶黄草中化学成分研究进展[J]. 中草药,2017,48(21):4571−4577. [ZHANG J, WU S M, YANG X, et al. Advances in chemical constituents of Penthorum chinense Pursh[J]. Chinese Traditional and Herbal Drugs,2017,48(21):4571−4577. doi: 10.7501/j.issn.0253-2670.2017.21.033
[17]	SUN X L, WU A G, BETTY Y K L, et al. The active components derived from Penthorum chinense Pursh protect against oxidative-stress-induced vascular injury via autophagy induction[J]. Free Radical Biology and Medicine,2020,146:160−180. doi: 10.1016/j.freeradbiomed.2019.10.417
[18]	袁叶飞, 白雪. 赶黄草总黄酮制备方法: 中国, 201811373488.7[P]. 2019-01-25 YUAN Y F, BAI X. Preparation method of total flavone of Penthorum chinense Pursh: China, 201811373488.7[P]. 2019-01-25.
[19]	付满玲, 九红, 袁叶飞. 赶黄草总黄酮对四氯化碳所致小鼠急性化学性肝损伤的保护作用[J]. 现代食品科技,2021,37(6):50−56. [FU M L, JIU H, YUAN Y F. Protective effect of total flavonoids of Penthorum chinense Pursh on acute chemical liver injury induced by carbon tetrachloride in mice[J]. Modern Food Science and Technology,2021,37(6):50−56. doi: 10.13982/j.mfst.1673-9078.2021.6.1054
[20]	王爽, 徐清华, 史保银, 等. 葛白复配颗粒对大鼠亚急性酒精性肝损伤的保护作用[J]. 毒理学杂志,2019,33(1):48−50. [WANG S, XU Q H, SHI B Y, et al. Protective effects of Gebai formulation granule on alcohol-induced hepatic injury in rats[J]. Journal of Toxicology,2019,33(1):48−50. doi: 10.16421/j.cnki.1002-3127.2019.01.008
[21]	苏林梁, 黄业宇, 冯丁山, 等. 槟榔花茶对大鼠亚急性酒精性肝损伤的保护作用[J]. 现代食品科技,2017,33(6):15−19. [SU L L, HUANG Y Y, FENG D S, et al. Protective effects of areca inflorescence tea on alcohol-induced hepatic injury in rats[J]. Modern Food Science and Technology,2017,33(6):15−19. doi: 10.13982/j.mfst.1673-9078.2017.6.003
[22]	QIAO J Y, LI H W, LIU F G, et al. Effects of Portulaca oleracea extract on acute alcoholic liver injury of rats[J]. Molecules,2019,24(16):2887−2910. doi: 10.3390/molecules24162887
[23]	叶文斌, 樊亮, 王昱, 等. 黄果槲寄生果实多糖对急性酒精肝损伤小鼠的保护作用[J]. 安徽农业大学学报,2017,44(2):218−223. [YE W B, FAN L WANG Y, et al. The protectionmechanism of Viscum coloratum (Kom.) Nakai f. lutescens Kitag fruit polysaccharides on acute alcoholic liver injury in mice[J]. Journal of Anhui Agricultural University,2017,44(2):218−223. doi: 10.13610/j.cnki.1672-352x.20170419.021
[24]	易宏伟, 朱笑笑, 黄小莉, 等. 富硒长双歧杆菌抑制乙醇诱导的小鼠肝损伤[J]. 中国药学杂志,2019,54(22):1859−1864. [YI H W, ZHU X X, HUANG X L, et al. Inhibition effect of selenium enriched Bifidobacterium longum on alcohol induced liver damage in mice[J]. Journal of Chinese Pharmaceutical Sciences,2019,54(22):1859−1864.
[25]	JIANG Z, CHEN C, WANG J, et al. Purple potato (Solanum tuberosum L.) anthocyaanins attenuate alcohol-induced hepatic injury by enhancing antioxidant defense[J]. Journal of Natural Medicines,2016,70(1):45−53. doi: 10.1007/s11418-015-0935-3
[26]	NIE G, ZHANG Y, ZHOU Z H, et al. Dynamic evaluation of the protective effect of Dendrobium officinale polysaccharide on acute alcoholic liver injury mice in vitro and in vivo by NIR fluorescence imaging[J]. Analytical and Bioanalytical Chemistry,2021,413(23):5715−5724. doi: 10.1007/s00216-021-03546-7
[27]	GOVINDAN S H, JAYABAL A G, SHANMUGAM J K, et al. Antioxidant and hepatoprotective effectsof Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats[J]. Food Science and Human Wellness,2021,10(4):523−535. doi: 10.1016/j.fshw.2021.04.015
[28]	GAO B, XU M J, BERTOLA A, et al. Animal models of alcoholic liver disease: Pathogenesis and clinical relevance[J]. Gene Express,2017,17(3):173−186. doi: 10.3727/105221617X695519
[29]	JAYARAMAN J, NAMASIVAYAM N. Naringenin modulates circulatory lipid peroxidation, antioxidant status and hepatic alcohol metabolizing enzymes in rats with ethanol induced liver injury[J]. Fundamental and Clinical Pharmacology,2011,25(6):682−689. doi: 10.1111/j.1472-8206.2010.00899.x
[30]	FANG C, ZHOU Q W, LIU Q Y, et al. Crosstalk between gut microbiota and host lipid metabolism in a mouse model of alcoholic liver injury by chronic Baijiu or ethanol feeding[J]. Food & Function,2022,13(2):596−608.

Supplements (0)

Cited By

Cited by

Periodical cited type(3)

1.	李佩艳，苏娇，肖鑫鑫，党东阳，罗登林，韩四海. 草酸处理对采后银条贮藏品质与细胞壁降解的影响及其通径分析. 食品与发酵工业. 2025(04): 83-90 .
2.	张高鹏，曲珈莹，侯雪宁，赵薇，侯双迪，薛敏，刘霞，范艳丽. 不同高氧预处理时间对枸杞鲜果贮藏保鲜作用比较. 现代食品科技. 2023(07): 130-137 .
3.	李彤，魏超昆，张惠玲，魏兆军. 枸杞（Lycium barbarum L.）籽饼粕酶解及其美拉德肽盐的开发. 中国食品添加剂. 2022(12): 62-74 .