Effects of Rhinacanthins-rich Extract from <i>Rhinacanthus nasutus</i> on Nonalcoholic Fatty Liver Rats Induced by High Fat Diet

GONG Zhiqiang; XIA Yuhong; HAN Sha; HUANG Yanhong; HUO Yu; CHEN Xiaolan; MENG Tianxiu; YANG Lifang

doi:10.13386/j.issn1002-0306.2022030138

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 389-397. > DOI: 10.13386/j.issn1002-0306.2022030138

GONG Zhiqiang, XIA Yuhong, HAN Sha, et al. Effects of Rhinacanthins-rich Extract from Rhinacanthus nasutus on Nonalcoholic Fatty Liver Rats Induced by High Fat Diet[J]. Science and Technology of Food Industry, 2023, 44(1): 389−397. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030138.

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Effects of Rhinacanthins-rich Extract from Rhinacanthus nasutus on Nonalcoholic Fatty Liver Rats Induced by High Fat Diet

1.
School of Chemistry and Chemical Engineering, Guangxi Minzu University, Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Nanning 530008, China
2.
Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530222, China

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Received Date: March 10, 2022
Available Online: October 28, 2022

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Abstract

Abstract

In order to evaluate the effect of RRE on reducing blood lipid and protecting liver, a model of nonalcoholic fatty liver disease (NAFLD) mice induced by high-fat diet was used, and then the body weight, liver weight and liver coefficient of each group were calculated, and the TC、TG、HDL-C、LDL-C、AST、ALT、AKP were checked by sample test. The liver tissue was stained with HE and oil Red O for pathological observation and fat particles observation; Animal experiment results showed that RRE intervention could effectively improve the process of liver pathological changes, alleviate inflammatory injury and reduce the accumulation of liver fat particles in NAFLD mice; both high and medium doses of RRE could significantly reduce the levels of TC, TG and LDL-C in serum of NAFLD mice (P<0.05), significantly increase the level of HDL-C (P<0.01), and significantly reduce the activities of AST, ALT and AKP in serum of NAFLD mice (P<0.05 or P<0.01); The index levels were better than those of ursolic acid group; RRE low dose group could significantly reduce the levels of TG, LDL-C, AST and in serum of NAFLD mice (P<0.05), and could reduce the levels of TC, ALT and AKP, but there was no significant difference (P>0.05); Through network pharmacology, the intersection of Rhinacanthus nasutus and NAFLD disease-related targets was carried out, and the effective active substances and action mechanism of RRE were predicted combined with molecular docking technology, which showed that rhinacanthin-C had good binding potential with the intersection targets. The experimental results showed that the RRE could reduce blood lipid, improved liver lipid deposition and inflammatory injury in NAFLD mice, so as to protect the liver. Its mechanism may be that the active substance rhinacanthin-C can improve liver lipid deposition and metabolic disorder through AMPK-SREBP1c-SIRT1 signal pathway. The experimental results can provide a scientific basis for the development of Zhuang medicine Rhinacanthus nasutus as a national drug for reducing blood lipid and improving fatty liver.
- Rhinacanthus nasutus,
- rhinacanthins-rich extract,
- nonalcoholic fatty liver disease (NAFLD),
- hypolipidemic,
- liver protection

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References

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