Improvement Effect of Blueberry Anthocyanin on Liver and Kidney Injury in Type 2 Diabetic Mice

DANG Ya; YOU Li; YANG Binyan

doi:10.13386/j.issn1002-0306.2022020171

Volume 43 Issue 20

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(20): 387-394. > DOI: 10.13386/j.issn1002-0306.2022020171

DANG Ya, YOU Li, YANG Binyan. Improvement Effect of Blueberry Anthocyanin on Liver and Kidney Injury in Type 2 Diabetic Mice[J]. Science and Technology of Food Industry, 2022, 43(20): 387−394. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020171.

Citation:

PDF (6728 KB)

Improvement Effect of Blueberry Anthocyanin on Liver and Kidney Injury in Type 2 Diabetic Mice

DANG Ya^{1, 2, 3, ,},
YOU Li^{1, 2,},
YANG Binyan^{1, 2}

1.
College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
2.
Shaanxi Province Fruit Industry Administration Qinba Mountain Blueberry Research Institute, Hanzhong 723000, China
3.
Shaanxi Provincial Key Laboratory of Resource Biology, Hanzhong 723000, China

More Information

Received Date: February 20, 2022
Available Online: August 07, 2022

Graphical Abstract

Abstract

Abstract

Objective: To discuss the improvement effect of blueberry anthocyanins (BA) on liver and kidney tissue injury in type 2 diabetic mice. Methods: Type 2 diabetic mouse model was established by high-fat and high-sugar diet combined with intraperitoneal injection of Streptozocin (STZ). Established normal control group, diabetes mellitus group, metformin hydrochloride group (250 mg/kg), blueberry anthocyanin group low-dose, medium-dose and high-dose groups (100, 200 and 300 mg/kg), and continued feeding for 4 weeks. The mice were sacrificed. The organ index was measured, the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in serum and the content of serum creatinine (Scr) and urea nitrogen (BUN) were measured. Liver and kidney were obtained for determining, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) levels. Liver and kidney HE stained pathological sections were made to observe the liver and kidney histopathological changes. Results: BA significantly decreased liver and kidney organ index (P<0.05). The activities of ALT and AST in serum were inhibited (P<0.01), and the contents of Scr and BUN in serum were decreased (P<0.01). The activities of SOD and GSH-Px in liver and kidney tissues were increased (P<0.05), and the content of MDA was decreased. Histopathological observation showed that BA alleviated the pathological damage of these organs, and the higher the dose, the more obvious the effect. Conclusion: BA had strong antioxidant activity, could improve liver and kidney function, and also had a certain repair effect on liver and kidney damage caused by type 2 diabetes, so it could be used as a potential functional food to prevent diabetes and its complications.
- blueberry anthocyanins,
- type 2 diabetes,
- liver,
- kidney,
- mice,
- improvement effect

FullText(HTML)

References (36)

References

[1]	SUN H, SAEEDI P, KARURANGA S, et al. IDF diabetes atlas: Global, regional and country-level diabetes prevalence estimates for 2021 and projections for 2045[J]. Diabetes Res Clin Pract,2022,83:109119.
[2]	JONCZYK-SKORKA K, KOWALSKI J. The assessment of risk factors for glucose metabolism disorders[J]. Polski Merkuriusz Lekarski,2017,42(252):241−246.
[3]	CHO N H, SHAW J E, KARURANGA S, et al. IDF diabetes atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045[J]. Diabetes Research & Clinical Practice,2018,138:271−281.
[4]	李晓倩, 顾勇清, 薛莉, 等. 中医药治疗糖尿病肾病的临床研究进展[J]. 中医药临床杂志,2020,32(9):1606−1609. [LI X Q, GU Y, XUE L, et al. Clinical research progress of traditional Chinese medicine in the treatment of diabetic nephropathy[J]. Clinical Journal of Traditional Chinese Medicine,2020,32(9):1606−1609.
[5]	TAKIKAWA A, USUI I, FUJISAKA S, et al. Macrophage-specific hypoxia-inducible factor-1α deletion suppresses the development of liver tumors in high-fat diet-fed obese and diabetic mice[J]. Journal of Diabetes Investigation,2019,10(6):1411−1418. doi: 10.1111/jdi.13047
[6]	毛跟年, 何亚娟, 杨文娟, 等. 裂叶荨麻提取物对2型糖尿病小鼠模型糖脂代谢影响的研究[J]. 动物医学进展,2020,41(1):61−65. [MAO G N, HE Y J, YANG W J, et al. Effect of Urticaria schizophyllum extract on glycolipid metabolism in type 2 diabetic mouse model[J]. Progress in Veterinary Medicine,2020,41(1):61−65. doi: 10.3969/j.issn.1007-5038.2020.01.011
[7]	BIONDI B, KAHALY G J, ROBERTSON R P. Thyroid dysfunction and diabetes mellitus: Two closely associated disorders[J]. Endocrine Reviews,2019,40(3):789−824. doi: 10.1210/er.2018-00163
[8]	李卫霞, 陈剑华, 王艳芳, 等. 黑米与欧洲越橘花色苷对小鼠肝功及抗氧化作用对比分析[J]. 食品研究与开发,2020,41(23):51−55. [LI W X, CHEN J H, WANG Y F, et al. Comparative analysis of anthocyanins from black rice and european bilberry on function of liver and anti-oxidation in serum of mice[J]. Food Research and Development,2020,41(23):51−55.
[9]	刘贺, 贺阳, 谷家栋, 等. 山葡萄花青素对慢性酒精中毒小鼠认知障碍的改善作用[J]. 食品与机械,2021,37(8):163−168,200. [LIU H, HE Y, GU J D, et al. Effects of anthocyanins from vitis amurensis on cognitive impairment in mice[J]. Food & Machinery,2021,37(8):163−168,200.
[10]	FANG J. Bioavailability of anthocyanins[J]. Drug Metabolism Reviews,2014,46(4):508−520. doi: 10.3109/03602532.2014.978080
[11]	BRANDENBURG J P, GILES L V. Four days of blueberry powder supplementation lowers the blood lactate response to running but has no effect on time-trial performance[J]. International Journal of Sport Nutrition and Exercise Metabolism,2019,29(6):636−642. doi: 10.1123/ijsnem.2019-0040
[12]	韩鹏祥, 张蓓, 冯叙桥, 等. 蓝莓的营养保健功能及其开发利用[J]. 食品工业科技,2015,36(6):370−375,379. [HAN P X, ZHANG B, FENG X Q, et al. Nutritional and health functions of blueberry and its development and utilization[J]. Science and Technology of Food Industry,2015,36(6):370−375,379.
[13]	SI X, TIAN J L, SHU C, et al. Serum ceramide reduction by blueberry anthocyanin-rich extract alleviates insulin resistance in hyperlipidemia mice[J]. Journal of Agricultural and Food Chemistry,2020,68(31):8185−8194. doi: 10.1021/acs.jafc.0c01931
[14]	魏奇. 姬松茸多酚降血糖活性及其作用机制的研究[D]. 福州: 福建农林大学, 2020 WEI Q. Study on the hypoglycemic activity and its mechanism of polyphenol from Agaricus blazei Murrill' fruiting bodies[D]. Fuzhou: Fujian Agriculture and Forestry University, 2020.
[15]	牛强. 软枣猕猴桃枝条多糖的分离纯化及降血糖活性研究[D]. 佳木斯: 佳木斯大学, 2020 NIU Q. Study on the separation, purification and hypoglycemic activity of polysaccharides from the branches of actinidia argute[D]. Jiamusi: Jiamusi University, 2020.
[16]	李仪奎. 中药药理实验方法学[M]. 上海: 上海科学技术出版社, 2006 LI Y K. Experimental methodology of traditional Chinese medicine pharmacology[M]. Shanghai: Science and Technology Press, 2006.
[17]	王云威, 王景雪. 铁皮石斛多糖对2型糖尿病小鼠降糖降脂的作用[J]. 食品科学,2020,41(21):127−132. [WANG Y W, WANG J X. Effects of Dendrobium officinale polysaccharide on hypoglycemic and lipid-lowering effects in type 2 diabetic mice[J]. Food Science,2020,41(21):127−132. doi: 10.7506/spkx1002-6630-20191101-001
[18]	HAN Y, XU Q, HU J N, et al. Maltol, a food flavoring agent, attenuates acute alcohol-induced oxidative damage in mice[J]. Nutrients,2015,7(1):682−696. doi: 10.3390/nu7010682
[19]	LI W, LIU Y, WANG Z, et al. Platycodin D isolated from the aerial parts of Platycodon grandiflorum protects alcohol-induced liver injury in mice[J]. Food Function,2015,6(5):1418−1427. doi: 10.1039/C5FO00094G
[20]	孙宏莱, 刘悦, 刘德江, 等. 毛水苏多糖对糖尿病小鼠肾脏的保护作用[J]. 食品工业科技,2021,42(17):373−380. [SUN H L, LIU Y, LIU D J, et al. Protective effect of polysaccharides from Stachys baicalensis on kidneys of diabetic disease mice[J]. Science and Technology of Food Industry,2021,42(17):373−380.
[21]	李巧琳. 辣木叶提取物的制备、降血糖活性评价及降糖功效因子结构解析[D]. 广州: 华南理工大学, 2019 LI Q L. Preparation of Moringa oleifera leaf extract, evaluation of its hypoglycemic effect and structure characterization of the main hypoglycemic ingredient[D]. Guangzhou: South China University of Technology, 2019.
[22]	缪云萍, 陈爱瑛, 夏志国, 等. 角鲨烯对小鼠急性酒精性肝损伤的保护作用[J]. 食品工业科技,2015,36(16):364−377. [MIAO Y P, CHEN A Y, XIA Z G, et al. Protective effects of squalene on acute alcohol-induced liver injury in mice[J]. Science and Technology of Food Industry,2015,36(16):364−377.
[23]	LI Y, LIU Y, LIANG J, et al. Gymnemic acid ameliorates hyperglycemia through PI3K/AKT and AMPK-mediated signaling pathways in type 2 diabetes mellitus rats[J]. Journal of Agricultural and Food Chemistry,2019,67(47):13051−13060. doi: 10.1021/acs.jafc.9b04931
[24]	赵苗鑫. 红花黄色素对糖尿病肾病小鼠肾脏的保护作用及相关机制研究[D]. 锦州: 锦州医科大学, 2021 ZHAO M X. The study of the protective effect and relevant mechanism of safflower yellow on kidney in diabetic nephropathy mice[D]. Jinzhou: Jinzhou Medical University, 2021.
[25]	HUANG W, WANG Y, WANG J, et al. Clinical characteristics of 192 adult hemophagocytic lymphohistiocytosis[J]. Zhonghua Xue Ye Xue Za Zhi,2014,35(9):796−801.
[26]	TANGVARASITTICHAI S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus[J]. World Journal of Diabetes,2015,6(3):456−80. doi: 10.4239/wjd.v6.i3.456
[27]	FORBES J M, COUGHLAN M T, COOPER M E. Oxidative stress as a major culprit in kidney disease in diabetes[J]. Diabetes,2008,57(6):1446−1454. doi: 10.2337/db08-0057
[28]	CHEN X, QIAN L, WANG B, et al. Synergistic hypoglycemic effects of pumpkin polysaccharides and puerarin on type II diabetes mellitus mice[J]. Molecules,2019,24(5):955. doi: 10.3390/molecules24050955
[29]	宋娜, 苏东峰, 刘晓燕, 等. 麦冬多糖对糖尿病围绝经期大鼠血清SOD、GSH-Px、CAT、MDA水平的影响[J]. 东南大学学报(医学版),2019,38(6):979−984. [SONG N, SU D F, LIU X Y, et al. Effects of Ophiopogon japonicuson polysaccharide on serum SOD, GSH-Px, CAT and MDA in diabetic perimenopausal rats[J]. Journal of Southeast University (Medical Science Edition),2019,38(6):979−984.
[30]	李霄, 孙延平, 王秋红, 等. 商陆及其性味拆分组分对阿霉素肾病大鼠肾脏的保护作用研究[J]. 中医药学报,2021,49(6):12−17. [LI X, SUN Y P, WANG Q H, et al. Protective effect of radix phytolaccae and its components on kidney in rats with doxorubicin-induced nephropathy[J]. Acta Chinese Medicine and Pharmacology,2021,49(6):12−17.
[31]	BRIL F, MILLAN L, KALAVALAPALLI S, et al. Use of a metabolomic approach to non-invasively diagnose non-alcoholic fatty liver disease in patients with type 2 diabetes mellitus[J]. Diabetes Obesity and Metabolism,2018,20(7):1702−1709. doi: 10.1111/dom.13285
[32]	HAN P, SHAO M, GUO L, et al. Niclosamide ethanolamine improves diabetes and diabetic kidney disease in mice[J]. American Journal of Translational Research,2018,10(4):1071−1084.
[33]	LEE S, KEIRSEY K I, KIRKLAND R, et al. Blueberry supplementation influences the gut microbiota, inflammation, and insulin resistance in high-fat-diet-fed rats[J]. The Journal of Nutrition,2018,148(2):209−219. doi: 10.1093/jn/nxx027
[34]	景志行. 中国野生蓝莓及其总花青素保肝效果的研究[D]. 上海: 上海海洋大学, 2017 JIN Z H. Hepato protective effect of vaccinium and its uliginosum linn anthocyanins on liver injury[D]. Shanghai: Shanghai Ocean University, 2017.
[35]	DAS S K. Free radicals, antioxidants and nutraceuticals in health, disease & radiation biology. Preface[J]. Indian Journal of Biochemistry & Biophysics,2012,49(5):291−292.
[36]	THOMPSON K, PEDERRICK W, SANTHAKUMAR A B. Anthocyanins in obesity-associated thrombogenesis: A review of the potential mechanism of action[J]. Food & Function,2016,7(5):2169−2178.

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	张黎梅，汪云飞，陈艳，董天秋，马文思，陈娴. HPLC-DAD法测定养生茶中的双氯芬酸钠. 食品与发酵科技. 2025(01): 169-172 .
2.	李肖斐. 超高效液相色谱-串联质谱法测定酵素梅中12种酚汀（酚丁）、酚酞及其酯类衍生物或类似物. 食品安全导刊. 2025(07): 105-109 .

Other cited types(1)

Get Citation

PDF

XML

Article Metrics

Article views (232) PDF downloads (24) Cited by(3)

Science and Technology of Food Industry

Improvement Effect of Blueberry Anthocyanin on Liver and Kidney Injury in Type 2 Diabetic Mice

Abstract

References

Cited by

Periodical cited type(2)

Other cited types(1)

Catalog

Article Metrics

Export File

Citation

Format

Content