Characteristics of Water-soluble Pectin from Greengage and Its Anti-inflammatory Activity in LPS-induced RAW246.7 Cells

HOU Jiali; ZHU Zhenzhu; XIE Minhao; YANG Qian; LIU Qin

doi:10.13386/j.issn1002-0306.2022010013

Volume 43 Issue 19

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(19): 401-409. > DOI: 10.13386/j.issn1002-0306.2022010013

HOU Jiali, ZHU Zhenzhu, XIE Minhao, et al. Characteristics of Water-soluble Pectin from Greengage and Its Anti-inflammatory Activity in LPS-induced RAW246.7 Cells[J]. Science and Technology of Food Industry, 2022, 43(19): 401−409. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010013.

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Characteristics of Water-soluble Pectin from Greengage and Its Anti-inflammatory Activity in LPS-induced RAW246.7 Cells

College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China

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Received Date: January 03, 2022
Available Online: August 02, 2022

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Abstract

Abstract

In this study, the water soluble pectin (WSP) from greengage juice was extracted. The structure and composition of WSP were characterized by high performance liquid chromatography (HPLC), high performance gel filtration chromatography (HPGFC), Fourier transform infrared spectrometer (FT-IR), scanning electron microscope (SEM) and X-ray diffraction (XRD). And the anti-inflammatory activity of WSP in lipopolysaccharide (LPS)-induced RAW246.7 cells was evaluated by enzyme-linked immunosorbent assay (ELISA). The results showed that WSP was a low-methoxy pectin with DE value of 18.89%. The content of galacturonic acid (GalA) was 69.72%, and the galactose (Gal) was the predominant neutral sugar with the content of 18.36%. The molecular weight (Mw) of WSP was 353.73 kDa. WSP significantly inhibited the release of tumor necrosis factor α (TNF-α) (P<0.05), interferon-γ (IFN-γ) (P<0.05), interleukin-6 (IL-6) (P<0.001) and increasing the secretion of interleukin-10 (IL-10) (P<0.01). The effects of WSP on Janus kinase/signal transduction and transcription activator signaling pathway (JAK/STAT signaling pathway) were investigated by real-time quantitative PCR and Western blot in order to clarify the anti-inflammatory mechanism. Compared with LPS group, WSP significantly upregulated the mRNA expressions of JAK1 (P<0.01), JAK2 (P<0.05), JAK3 (P<0.001), STAT1 (P<0.001), STAT2 (P<0.001) and STAT3 (P<0.05). Further analysis showed that the phosphorylation levels of JAK1 and STAT3 were significantly inhibited by WSP treatment (P<0.05). Therefore, the WSP can alleviate LPS-induced inflammation in RAW264.7 cells by interfering with JAK/STAT signaling pathway and inhibiting the phosphorylation levels of JAK and STAT families related to inflammatory signaling. This study can provide a basis for the evaluation of the health improvement effect of greengage fruit and the development of value added greengage product.
- greengage,
- water-soluble pectin,
- RAW246.7 cells,
- anti-inflammatory,
- JAK/STAT pathway

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References

[1]	黄伟素, 潘秋月, 高一勇. 青梅果产品的开发现状和发展趋势[J]. 食品工业科技,2011,32(11):519−521,524. [HUANG W S, PAN Q Y, GAO Y Y. The development status and development trend of green plum fruit products[J]. Science and Technology of Food Industry,2011,32(11):519−521,524. HUANG W S, PAN Q Y, GAO Y Y. The development status and development trend of green plum fruit products[J]. Science and Technology of Food Industry, 2011, 32(11): 519−521, 524.
[2]	IGLESIAS-CARRES L, MAS-CAPDEVILA A, BRAVO F I, et al. Optimization of extraction methods for characterization of phenolic compounds in apricot fruit (Prunus armeniaca)[J]. Food & Function,2019,10(10):6492−6502.
[3]	马嫄, 罗鸣, 殷晓翠, 等. 果梅的化学成分及应用进展研究[J]. 食品工业科技,2018,29(4):337−341. [MA Y, LUO M, YIN X C, et al. Research on the chemical constituents and application progress of fruit plum[J]. Science and Technology of Food Industry,2018,29(4):337−341. MA Y, LUO M, YIN X C, et al. Research on the chemical constituents and application progress of fruit plum[J]. Science and Technology of Food Industry, 2018, 29(4): 337-341.
[4]	CUI J, ZHAO C, FENG L, et al. Pectins from fruits: Relationships between extraction methods, structural characteristics, and functional properties[J]. Trends in Food Science & Technology,2021,110:39−54.
[5]	HOSSEINI S S, KHODAIYAN F, KAZEMI M, et al. Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method[J]. International Journal of Biological Macromolecules,2019,125(15):621−629.
[6]	NI L Y, WANG L, FU X T, et al. In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica[J]. International Journal of Biological Macromolecules,2020,156:717−729. doi: 10.1016/j.ijbiomac.2020.04.012
[7]	任多多, 江伟, 孙印石, 等. 果胶的分类、功能及其在食品工业中应用的研究进展[J]. 食品工业科技,2022,43(3):438−446. [REN D D, JIANG W, SUN Y S, et al. Research progress on classification, function and application of pectin in food industry[J]. Science and Technology of Food Industry,2022,43(3):438−446. doi: 10.13386/j.issn1002-0306.2021020069 REN D D, JIANG W, SUN Y S, et al. Research progress on classification, function and application of pectin in food industry[J]. Science and Technology of Food Industry, 2022, 43(3): 438-446. doi: 10.13386/j.issn1002-0306.2021020069
[8]	HO Y Y, LIN C M, WU M C. Evaluation of the prebiotic effects of citrus pectin hydrolysate[J]. Journal of Food and Drug Analysis,2017,25(3):550−558. doi: 10.1016/j.jfda.2016.11.014
[9]	XIONG L, OUYANG K H, JIANG Y, et al. Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage[J]. International Journal of Biological Macromolecules:Structure,2018,107(Pt.B):1898−1907.
[10]	YEUNG Y K, KANG Y R, BO R S, et al. Structural, antioxidant, prebiotic and anti-inflammatory properties of pectic oligosaccharides hydrolyzed from okra pectin by Fenton reaction[J]. Food Hydrocolloids,2021,118(3-4):106779.
[11]	LI X, WEI Z, WANG X, et al. Premna microphylla Turcz leaf pectin exhibited antioxidant and anti-inflammatory activities in LPS-stimulated RAW 264.7 macrophages[J]. Food Chemistry,2021,349(6494):129164.
[12]	ZHANG H, GUO Q F, LIANG Z H, et al. Anti-inflammatory and antioxidant effects of Chaetoglobosin V_B in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways[J]. Food and Chemical Toxicology,2021,147:111915. doi: 10.1016/j.fct.2020.111915
[13]	岳彩霞, 陈磊, 王文魁, 等. 山楂酸调控STAT3磷酸化保护LPS致RAW264.7细胞炎症反应的研究[J]. 食品与机械,2021,37(6):36−42. [YUE C X, CHEN L, WANG W K, et al. Maslinic acid regulates STAT3 phosphorylation to protect LPS-induced RAW264.7 cell inflammatory response[J]. Food and Machinery,2021,37(6):36−42. YUE C X, CHEN L, WANG W K, et al. Maslinic acid regulates STAT3 phosphorylation to protect LPS-induced RAW264.7 cell inflammatory response[J]. Food and Machinery, 2021, 37(6): 36-42.
[14]	LE B, PHAM T N A, YANG S H. Prebiotic potential and anti-inflammatory activity of soluble polysaccharides obtained from soybean residue[J]. Foods,2020,9(1808):1−13.
[15]	TAN H D, CHEN W, LIU Q S, et al. Pectin oligosaccharides ameliorate colon cancer by regulating oxidative stress- and inflammation-activated signaling path ways[J]. Frontiers in Immunology,2018,9:1504. doi: 10.3389/fimmu.2018.01504
[16]	WELLALA C, BI J F, LIU X, et al. Juice related water-soluble pectin characteristics and bioaccessibility of bioactive compounds in oil and emulsion incorporated mixed juice processed by high pressure homogenization[J]. Food Hydrocolloids,2019,93:56−67. doi: 10.1016/j.foodhyd.2019.02.011
[17]	ZHOU L, WANG Y, LIU F, et al. Effect of high pressure carbon dioxide on the properties of water soluble pectin in peach juice[J]. Food Hydrocolloids,2014,40:173−181. doi: 10.1016/j.foodhyd.2014.02.016
[18]	ZHANG L, YE X, DING T, et al. Ultrasound effects on the degradation kinetics, structure and rheological properties of apple pectin[J]. Ultrason Sonochem,2013,20(1):222−231. doi: 10.1016/j.ultsonch.2012.07.021
[19]	FANG Y, XU Z, SHI Y, et al. Protection mechanism of Se-containing protein hydrolysates from Se-enriched rice on Pb²⁺-induced apoptosis in PC12 and RAW264.7 cells[J]. Food Chemistry,2017,219:391−398. doi: 10.1016/j.foodchem.2016.09.131
[20]	HAMMER K, YUM M Y, DIXON P M, et al. Identification of JAK-STAT pathways as important for the anti-inflammatory activity of a Hypericum perforatum fraction and bioactive constituents in RAW 264.7 mouse macrophages[J]. Phytochemistry,2010,71(7):716−725. doi: 10.1016/j.phytochem.2010.02.006
[21]	ZHOU M, BI J F, CHEN J, et al. Impact of pectin characteristics on lipid digestion under simulated gastrointestinal conditions: Comparison of water-soluble pectins extracted from different sources[J]. Food Hydrocolloids,2021,112:106350. doi: 10.1016/j.foodhyd.2020.106350
[22]	LI Y, DENG Y, LI Z, et al. Composition physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins[J]. International Journal of Biological Macromolecules,2020,165:2630−2639. doi: 10.1016/j.ijbiomac.2020.10.167
[23]	MARCELIN O, SAULNIER L, BRILLOUET J M. Extraction and characterisation of water-soluble pectic substances from guava (Psidium guajava L.)[J]. Carbohydrate Research,1991,212(91):159−167.
[24]	JIN M Y, LI M Y, HUANG R M, et al. Structural features and anti-inflammatory properties of pectic polysaccharides: A review[J]. Trends in Food Science & Technology,2021,107(11):284−298.
[25]	HE L, YAN X T, LIANG J, et al. Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem[J]. Carbohydrate Polymers,2018,198(15):101−108.
[26]	JIANG Y, DU J H. Properties of high-methoxyl pectin extracted from “Fuji” apple pomace in China[J]. Journal of Food Process Engineering,2017,40(3):12497. doi: 10.1111/jfpe.12497
[27]	GNANASAMBANDAM R, PROCTOR A. Determination of pectin degree of esterification by diffuse reflectance Fourier transform infrared spectroscopy-quantitative evaluation of uronic acid and acetamidodeoxyhexose moieties[J]. Food Chemistry,2000,68(3):327−332. doi: 10.1016/S0308-8146(99)00191-0
[28]	PAN X, ZHAO W, WANG Y, et al. Physicochemical and structural properties of three pectin fractions from muskmelon (Cucumis melo) and their correlation with juice cloud stability[J]. Food Hydrocolloids, 2022, 124, Part B, 107313.
[29]	XIE Z L, WANG Y, HUANG J Q, et al. Anti-inflammatory activity of polysaccharides from Phellinus linteus by regulating the NF-κB translocation in LPS-stimulated RAW264.7 macrophages[J]. International Journal of Biological Macromolecules,2019,129:61−67. doi: 10.1016/j.ijbiomac.2019.02.023
[30]	WU D, CHEN S, YE X, et al. Enzyme-extracted raspberry pectin exhibits a high-branched structure and enhanced anti-inflammatory properties than hot acid-extracted pectin[J]. Food Chemistry,2022,383(30):132387.
[31]	POPOV S V, MARKOV P A, POPOVA G Y, et al. Anti-inflammatory activity of low and high methoxylated citrus pectins[J]. Biomedicine & Preventive Nutrition,2013,3(1):59−63.
[32]	CAO J, YANG J, WANG Z M, et al. Modified citrus pectins by UV/H₂O₂ oxidation at acidic and basic conditions: Structures and in vitro anti-inflammatory, anti-proliferative activities[J]. Carbohydrate Polymers,2020,247:116742. doi: 10.1016/j.carbpol.2020.116742
[33]	MOHR A, CHATAIN N, DOMOSZLAI T, et al. Dynamics and non-canonical aspects of JAK/STAT signalling[J]. European Journal of Cell Biology,2012,91(6-7):524−532. doi: 10.1016/j.ejcb.2011.09.005
[34]	ZHOU M J, GUO C H, LI X, et al. JAK/STAT signaling controls the fate of CD8+CD103+tissue-resident memory T cell in lupus nephritis[J]. Journal of Autoimmunity,2020,109:102424. doi: 10.1016/j.jaut.2020.102424
[35]	SALAS A, HERNANDEZ R C, DUIJVESTEIN M, et al. JAK-STAT pathway targeting for the treatment of inflammatory bowel disease[J]. Nature Reviews Gastroenterology & Hepatology,2020,17:323−337.
[36]	MA Y, TANG T, SHENG L, et al. Aloin suppresses lipopolysaccharide induced inflammation by inhibiting JAK1-STAT1/3 activation and ROS production in RAW264.7 cells[J]. International Journal of Molecular Medicine,2018,42(4):1925−1934.
[37]	MAO S, PARK Y, HASEGAWA Y, et al. Intrinsic apoptotic pathways of gingival epithelial cells modulated by Porphyromonas gingivalis[J]. Cellular Microbiology,2007,9(8):1997−2007. doi: 10.1111/j.1462-5822.2007.00931.x

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