Optimization of Extraction Process of Aqueous Extract of <i>Rehmannia glutinosa</i> Based on Entropy Weight Method in Cooperation with Analytic Hierarchy Process and Back Propagation Neural Network with Multiple Indicators

ZHU Ziyu; XIE Yuxin; YU Yueting; ZHANG Mei

doi:10.13386/j.issn1002-0306.2021120331

Volume 43 Issue 19

Sep. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(19): 264-272. > DOI: 10.13386/j.issn1002-0306.2021120331

ZHU Ziyu, XIE Yuxin, YU Yueting, et al. Optimization of Extraction Process of Aqueous Extract of Rehmannia glutinosa Based on Entropy Weight Method in Cooperation with Analytic Hierarchy Process and Back Propagation Neural Network with Multiple Indicators[J]. Science and Technology of Food Industry, 2022, 43(19): 264−272. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120331.

Citation:

PDF (3216 KB)

Optimization of Extraction Process of Aqueous Extract of Rehmannia glutinosa Based on Entropy Weight Method in Cooperation with Analytic Hierarchy Process and Back Propagation Neural Network with Multiple Indicators

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610000, China

More Information

Received Date: January 02, 2022
Available Online: July 28, 2022

Graphical Abstract

Abstract

Abstract

Objective: To optimize the ultrasonic extraction process of aqueous extract of Rehmannia glutinosa based on entropy weight method (EWM) together with analytic hierarchy process (AHP) and back propagation neural network (BPNN). Methods: Taking ultrasonic temperature, ultrasonic time, solid-liquid ratio as orthogonal test factors, catalpa, rehmannioside D, ajugol, rehmannioside polysaccharide content, and the yield of water-soluble extract as indexes, EWM-AHP method was used to determine the weight of each index, to obtain the comprehensive score of multiple indexes. Then, the experimental process conditions were used as the input and the synthesis score was used as the output to build and train a BPNN model to find the best process for the extraction process of Rehmannia glutinosa. Results: The preferred process of BPNN was extraction at 60 ℃ with 33 times of water for 70 min. The mean values of catalpa, rehmannioside D, ajugol, rehmannioside polysaccharide content, and the yield of water-soluble extract were 17.04, 3.75, 10.57, 24.86 mg/g and 0.82 g/g, respectively, with a combined score of 97.74; The best process of the orthogonal test was extraction at 50 ℃ with 25 times of water for 1 h. The mean values of catalpa, rehmannioside D, ajugol, rehmannioside polysaccharide content, and the yield of water-soluble extract were 12.72, 2.58, 8.20, 25.02 mg/g and 0.80 g/g, respectively, with a combined score of 97.26. Conclusion: This study determined that the BPNN preferred process was optimal and provided a reference for the application of the EWM-AHP method combined with BPNN in the extraction process.
- Rehmannia glutinosa,
- extraction process,
- back propagation neural network,
- entropy weighting method,
- analytic hierarchy process

FullText(HTML)

References (31)

References

[1]	张维方, 樊克锋, 雷敬卫, 等. 地黄药材的红外指纹图谱及多元统计分析[J]. 光谱学与光谱分析,2021,41(11):3392−3398. [ZHANG W F, FAN K F, LEI J W, et al. Infrared fingerprint and multivariate statistical analysis of Rehmannia glutinosa[J]. Spectroscopy and Spectral Analysis,2021,41(11):3392−3398. ZHANG W F, FAN K F, LEl J W, et al. Infrared fingerprint and multivariate statistical analysis of Rehmannia glutinosa[J]. Spectroscopy and Spectral Analysis, 2021, 41(11): 3392-3398.
[2]	THU V K, THOA N T, HIEN N T T, et al. Iridoid glycosides link with phenylpropanoids from Rehmannia glutinosa[J]. Natural Product Research,2021(5):1−6.
[3]	徐卓, 戴新新, 宿树兰, 等. 基于UPLC-TQ-MS的地黄中核苷类和氨基酸类成分动态积累研究[J]. 中草药,2021,52(23):7323−7330. [XU Z, DAI X X, XIU S L, et al. Analysis of dynamic accumulation of nucleosides and amino acids in Rehmannia glutinosa based on UPLC-TQ-MS[J]. Chinese Traditional and Herbal Drug,2021,52(23):7323−7330. doi: 10.7501/j.issn.0253-2670.2021.23.027 XU Z, DAI X X, XIU S L, et al. Analysis of dynamic accumulation of nucleosides and amino acids in Rehmannia glutinosa based on UPLC-TQ-MS[J]. Chinese Traditional and Herbal Drug, 2021, 52(23): 7323-7330. doi: 10.7501/j.issn.0253-2670.2021.23.027
[4]	汪程远, 张浩, 孟莉, 等. 大孔吸附树脂分离纯化生地黄中苷类与糖类[J]. 中药材,2003(3):202−204. [WANG C Y, SUN H, MENG L, et al. Separation and purification of polysaccharides from Rehmannia glutinosa by macroporous adsorption resin[J]. Journal of Chinese Medicinal Materials,2003(3):202−204. doi: 10.3321/j.issn:1001-4454.2003.03.022 WANG C Y, SUN H, MENG L, et al. Separation and purification of polysaccharides from Rehmannia glutinosa by macroporous adsorption resin[J]. Journal of Chinese Medicinal Materials, 2003(3): 202-204. doi: 10.3321/j.issn:1001-4454.2003.03.022
[5]	陈金鹏, 张克霞, 刘毅, 等. 地黄化学成分和药理作用的研究进展[J]. 中草药,2021(6):1772−1784. [CHEN J P, ZHANG K X, LIU Y, et al. Research progress on chemical constituents and pharmacological actions of Rehmannia glutinosa[J]. Chinese Traditional and Herbal Drug,2021(6):1772−1784. doi: 10.7501/j.issn.0253-2670.2021.06.028 CHEN J P, ZHANG K X, LIU Y, et al. Research progress on chemical constituents and pharmacological actions of Rehmannia glutinosa[J]. Chinese Traditional and Herbal Drug, 2021(6): 1772-1784. doi: 10.7501/j.issn.0253-2670.2021.06.028
[6]	JO R, MIN J R, JEONG S H. Anti-diabetic effects of water extracts of Rehmannia glutinosa Libosch root in 3T3-L1 adipocytes and C57BL/KsJ-db/db mice[J]. Journal of the Korean Society of Food Science and Nutrition,2018,47(10):957−965. doi: 10.3746/jkfn.2018.47.10.957
[7]	TSENG Y T, CHANG W H, LIN C C, et al. Protective effects of Liuwei dihuang water extracts on diabetic muscle atrophy[J]. Phytomedicine, 2018, 53: 96-106.
[8]	ZHOU X D, SHI D D, ZHANG Z J, et al. Ameliorative effects of Radix rehmanniae extract on the anxiety and depression like symptoms in ovariectomized mice: A behavioral and molecular study[J]. Phytomedicine,2019,63:153012. doi: 10.1016/j.phymed.2019.153012
[9]	GONG W, ZHANG N D, CHENG G, et al. Rehmannia glutinosa Libosch extracts prevent bone loss and architectural deterioration and enhance osteoblastic bone formation by regulating the IGF-1/PI3K/mTOR pathway in streptozotocin-induced diabetic rats[J]. International Journal of Molecular Sciences,2019,20(16):3964. doi: 10.3390/ijms20163964
[10]	曹瑞, 张红, 师延琼, 等. 响应面法分析优化生地黄提取工艺研究[J]. 医学研究杂志,2014,43(2):69−72. [CAO R, ZHANG H, SHI Y Q, et al. Optimization of extraction technology of Rehmannia glutinosa by response surface methodology[J]. Journal of Medical Research,2014,43(2):69−72. CAO R, ZHANG H, SHI Y Q, et al. Optimization of extraction technology of Rehmannia glutinosa by response surface methodology[J]. Journal of Medical Research, 2014, 43(2): 69-72.
[11]	熊辉, 朱军, 陈琴华. 地黄不同提取工艺中梓醇含量的比较[J]. 中国医院药学杂志,2015,35(17):1610−1612. [XIONG H, ZHU J, CHEN Q H. Comparison of contents of catalpol in Radix Rehmanniae by different extraction methods[J]. Chinese Journal of Hospital Pharmacy,2015,35(17):1610−1612. doi: 10.13286/j.cnki.chinhosppharmacyj.2015.17.21 XIONG H, ZHU J, CHEN Q H. Comparison of contents of catalpol in Radix Rehmanniae by different extraction methods[J]. Chinese Journal of Hospital Pharmacy, 2015, 35(17): 1610-1612. doi: 10.13286/j.cnki.chinhosppharmacyj.2015.17.21
[12]	王慧森, 刘明, 李更生, 等. 鲜地黄提取物中3种原型入血成分的含量测定[J]. 中国实验方剂学杂志,2013,19(12):66−70. [WANG H S, LIU M, LI G S, et al. Determination for three prototype constituents into the blood from extract of fresh Rehmannia qlutinosa[J]. Chinese Journal of Experimental Traditional Medical Formulae,2013,19(12):66−70. [WANG H S, LIU M, LI G S, et al. Determination for three prototype constituents into the blood from extract of fresh Rehmannia qlutinosa[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2013, 19(12): 66-70].
[13]	BHATTAMISRA S K, KOH H M, LIM S Y, et al. Molecular and biochemical pathways of catalpol in alleviating diabetes mellitus and its complications[J]. Biomolecules,2021,11(2):27.
[14]	BI J J, LI J, CHEN S J, et al. Catalpol ameliorates type II collagen-induced arthritis in rats and inhibits LPS-stimulated inflammatory response in SW982 human synovial cells[J]. Journal of Functional Foods,2019,59:291−300. doi: 10.1016/j.jff.2019.05.050
[15]	田平平, 潘颖, 施维, 等. 地黄中益母草苷对食管癌细胞增殖抑制作用的研究[J]. 园艺与种苗,2021,41(10):13−15,17. [TIAN P P, PAN Y, SHI W, et al. Inhibitory effect of ajugol in Rehmannia qlutinosa on the proliferation of esophageal carcinoma cells[J]. Horticulture & Seed,2021,41(10):13−15,17. TIAN P P, PAN Y, SHI W, et al. Inhibitory effect of ajugol in Rehmannia qlutinosa on the proliferation of esophageal carcinoma cells[J]. Horticulture & Seed, 2021, 41(10): 13-15, 17.
[16]	ZHOU J, XU G, YAN J Y, et al. Rehmannia glutinosa (Gaertn. ) DC. polysaccharide ameliorates hyperglycemia, hyperlipemia and vascular inflammation in streptozotocin-induced diabetic mice[J]. Journal of Ethnopharmacology,2015,164:229−238. doi: 10.1016/j.jep.2015.02.026
[17]	高帅, 泮思林, 杜占慧, 等. 地黄多糖抑制大鼠主动脉血管钙化的实验研究[J]. 中华实验外科杂志,2021(8):1466−1469. [GAO S, PAN S L, DU Z H, et al. Inhibitory effect of Rehmannia glutinosa polysaccharide on aortic calcification in rats[J]. Chinese Journal of Experimental Surgery,2021(8):1466−1469. doi: 10.3760/cma.j.cn421213-20210116-00057 GAO S, PAN S L, DU Z H, et al. Inhibitory effect of Rehmannia glutinosa polysaccharide on aortic calcification in rats[J]. Chinese Journal of Experimental Surgery, 2021(8): 1466-1469. doi: 10.3760/cma.j.cn421213-20210116-00057
[18]	潘小红, 李涛, 殷帅, 等. 超声波辅助提取对地黄中药材及成方制剂含量测定的影响[J]. 中医药导报,2020,26(8):12−15,23. [PAN X H, LI T, YIN S, et al. Effect of ultrasonic-assisted extraction on the content determination of dihuang (Radix Rehmanniae) and its preparations[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy,2020,26(8):12−15,23. PAN X H, LI T, YIN S, et al. Effect of ultrasonic-assisted extraction on the content determination of dihuang (Radix Rehmanniae) and its preparations[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2020, 26(8): 12-15, 23.
[19]	张红果, 张汝学, 贾正平, 等. 正交实验法优选地黄寡糖有效部位的提取工艺[J]. 中国实验方剂学杂志,2010,16(5):27−29. [ZHANG H G, ZHANG R X, JIA Z P, et al. Orthogonal experiment was used to optimize the extraction process of oligosaccharide from Rehmannia glutinosa[J]. Chinese Journal of Experimental Traditional Medical Formulae,2010,16(5):27−29. doi: 10.3969/j.issn.1005-9903.2010.05.007 ZHANG H G, ZHANG R X, JIA Z P, et al. Orthogonal experiment was used to optimize the extraction process of oligosaccharide from Rehmannia glutinosa[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2010, 16(5): 27-29. doi: 10.3969/j.issn.1005-9903.2010.05.007
[20]	尤游, 张林静. 基于组合赋权和BPNN的高校学生综合素质评价研究[J]. 安徽电子信息职业技术学院学报,2021,20(5):5−9. [YOU Y, ZHANG L J. Research on comprehensive quality evaluation of college students based on combined weight and BP neural network[J]. Journal of Anhui Yocatlonal College of Electronic& Information Technology,2021,20(5):5−9. doi: 10.3969/j.issn.1671-802X.2021.05.002 YOU Y, ZHANG L J. Research on comprehensive quality evaluation of college students based on combined weight and BP neural network[J]. Journal of Anhui Yocatlonal College of Electronic& Information Technology, 2021, 20(5): 5-9. doi: 10.3969/j.issn.1671-802X.2021.05.002
[21]	黄露, 徐顶巧, 乐世俊, 等. 基于层次分析-熵权法和网络药理学的野马追质量标志物研究[J]. 中国中药杂志,2021,46(21):5545−5554. [HUANG L, XU D Q, LE S J, et al. Research on Q-markers of Eupatorium lindleyanum based on analytic hierarchy process-entropy weight method and network pharmacology[J]. China Journal of Chinese Materia Medica,2021,46(21):5545−5554. HUANG L, XU D Q, LE S J, et al. Research on Q-markers of Eupatorium lindleyanum based on analytic hierarchy process-entropy weight method and network pharmacology[J]. China Journal of Chinese Materia Medica, 2021, 46(21): 5545-5554.
[22]	谢平, 魏海峰, 温仁华, 等. 基于BPNN-遗传算法和信息熵理论优化凉粉草煎煮提取工艺[J]. 中国中医药信息杂志,2021,29(2):86−92. [XIE P, WEI H F, WEN R H, et al. Optimization on extraction process of Mesona chinensis Benth. based on BP neural network genetic algorithm and information entropy theory[J]. Chinese Journal of Information on TCM,2021,29(2):86−92. XIE P, WEI H F, WEN R H, et al. Optimization on extraction process of Mesona chinensis Benth. based on BP neural network genetic algorithm and information entropy theory[J]. Chinese Journal of Information on TCM, 2021, 29(2): 86-92.
[23]	张晓萍, 张建民. 基于BPNN的复合型挑坎体型参数反演研究[J]. 水利水电技术(中英文),2021,53(4):138−147. [ZHANG X P, ZHANG J M. Study on shape parameter inversion of compound bucket based on BP neural network[J]. Water Resources and Hydropower Engineering,2021,53(4):138−147. ZHANG X P, ZHANG J M. Study on shape parameter inversion of compound bucket based on BP neural network[J]. Water Resources and Hydropower Engineering, 2021, 53(4): 138-147.
[24]	张留记, 王建霞, 屠万倩, 等. 生地黄与熟地黄中5个苷类成分和总多糖的含量比较[J]. 天然产物研究与开发,2019,31(4):566−571. [ZHANG L J, WANG J X, TU W Q, et al. Comparation of the contents of 5 glycosides and total polysaccharides in Rehmanniae Radix and Rehmanniae Radix Praeparata[J]. Natural Product Research and Development,2019,31(4):566−571. ZHANG L J, WANG J X, TU W Q, et al. Comparation of the contents of 5 glycosides and total polysaccharides in Rehmanniae Radix and Rehmanniae Radix Praeparata[J]. Natural Product Research and Development, 2019, 31(4): 566-571.
[25]	张骆琪, 刘苏伟, 王飞, 等. 地黄多糖超声提取工艺优化及其抗氧化活性[J]. 中成药,2018,40(12):2662−2667. [ZHANG L Q, LIU S W, WANG F, et al. Polysaccharides from Rehmannia glutinosa, their ultrasonic extraction process optimization and antioxidant activity[J]. Chinese Traditional Patent Medicine,2018,40(12):2662−2667. doi: 10.3969/j.issn.1001-1528.2018.12.010 ZHANG L Q, LIU S W, WANG F, et al. Polysaccharides from Rehmannia glutinosa, their ultrasonic extraction process optimization and antioxidant activity[J]. Chinese Traditional Patent Medicine, 2018, 40(12): 2662-2667. doi: 10.3969/j.issn.1001-1528.2018.12.010
[26]	扈本荃, 徐玥, 吴曼, 等. 地黄饮超声提取工艺的研究[J]. 化学工程师,2021,35(12):71−73,19. [HU B Q, XU Y, WU M, et al. Study on the medthod for ultrasonic extraction of Dihuang Yin by orthogonal experiment[J]. Chemical Engineer,2021,35(12):71−73,19. HU B Q, XU Y, WU M, et al. Study on the medthod for ultrasonic extraction of Dihuang Yin by orthogonal experiment[J]. Chemical Engineer, 2021, 35(12): 71-73, 19.
[27]	祁小妮, 林楠楠, 李振亮, 等. 生地黄多糖提取工艺的优化及抗氧化活性研究[J]. 食品与发酵工业,2016,42(2):231−235. [QI X N, LIN N N, LI Z L, et al. Optimization of Rehmannia glutinosa polysaccharide extraction process and antioxidant activity study[J]. Food and Fermentation Industries,2016,42(2):231−235. QI X N, LIN N N, LI Z L, et al. Optimization of Rehmannia glutinosa polysaccharide extraction process and antioxidant activity study[J]. Food and Fermentation Industries, 2016, 42(2): 231-235.
[28]	国家药典委员会. 中华人民共和国药典. 四部[S]. 北京: 中国医药科技出版社, 2020. National Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Four Parts[S]. Beijing: China Pharmaceutical Science and Technology Press, 2020.
[29]	刘路路, 李可, 韦智江, 等. 基于响应面法和熵权法优化连翘叶中连翘酯A、连翘苷和芦丁提取工艺[J]. 食品工业科技,2022,43(2):166−173. [LIU L L, LI K, WEI Z J, et al. Optimization of extraction process of forsythioside A, forsythin and rutin from Forsythia suspensa leaves based on response surface methodology and entropy weight method[J]. Science and Technology of Food Industry,2022,43(2):166−173. LIU L L, LI K, WEI Z J, et al. Optimization of extraction process of forsythioside A, forsythin and rutin from Forsythia suspensa leaves based on response surface methodology and entropy weight method[J]. Science and Technology of Food Industry, 2022, 43(2): 166-173.
[30]	钱怡洁, 皮文霞, 朱广飞, 等. 基于熵权法结合层次分析法和反向传播神经网络优选酒萸肉蒸制工艺[J]. 中草药,2021,52(22):6816−6824. [QIAN Y J, PI W X, ZHU G F, et al. Optimization of processing technology of wine-steamed Corni Fructus based on entropy method combined with analytic hierarchy process and BP neural network[J]. Chinese Traditional and Herbal Drugs,2021,52(22):6816−6824. doi: 10.7501/j.issn.0253-2670.2021.22.006 QIAN Y J, PI W X, ZHU G F, et al. Optimization of processing technology of wine-steamed Corni Fructus based on entropy method combined with analytic hierarchy process and BP neural network[J]. Chinese Traditional and Herbal Drugs, 2021, 52(22): 6816-6824. doi: 10.7501/j.issn.0253-2670.2021.22.006
[31]	田春莲, 丁文. 湖北地黄梓醇超声波提取及含量测定[J]. 安徽农业科学,2009,37(28):13788−13789,13792. [TIAN C L, DING W. Ultrasonic extraction and content determination of Rehmannia glutinosa in Hubei[J]. Journal of Anhui Agricultural Sciences,2009,37(28):13788−13789,13792. doi: 10.3969/j.issn.0517-6611.2009.28.133 TIAN C L, DING W. Ultrasonic extraction and content determination of Rehmannia glutinosa in Hubei[J]. Journal of Anhui Agricultural Sciences, 2009, 37(28): 13788-13789, 13792. doi: 10.3969/j.issn.0517-6611.2009.28.133

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	陈星星. 水产品中重金属分析技术的研究进展. 浙江农业科学. 2023(06): 1404-1407 .
2.	闫奕霏，高薪，汤修映. 基于近红外漫反射光谱的面包老化过程中非冻结水含量无损检测. 食品安全质量检测学报. 2022(22): 7264-7271 .