Optimization of Liquid Fermentation Medium Formulation of <i>Morchella eohespera</i> Exopolysaccharide and Its Hypoglycemic Activity<i> in Vitro</i>

LIN Shan; ZHAO Ping; FU Yunna; LIU Bing; FENG Peiyao; LI Haozhe; XIA Xingxing

doi:10.13386/j.issn1002-0306.2021120191

Volume 43 Issue 20

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(20): 196-203. > DOI: 10.13386/j.issn1002-0306.2021120191

LIN Shan, ZHAO Ping, FU Yunna, et al. Optimization of Liquid Fermentation Medium Formulation of Morchella eohespera Exopolysaccharide and Its Hypoglycemic Activity in Vitro[J]. Science and Technology of Food Industry, 2022, 43(20): 196−203. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120191.

Citation:

PDF (5626 KB)

Optimization of Liquid Fermentation Medium Formulation of Morchella eohespera Exopolysaccharide and Its Hypoglycemic Activity in Vitro

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China

More Information

Received Date: December 19, 2021
Available Online: August 10, 2022

Graphical Abstract

Abstract

Abstract

In this study, strains of wild Morchella eohespera in Gansu province were selected as the research object to explore the optimal solution of liquid fermentation medium of M. eohespera extracellular polysaccharide and its hypoglycemic activity in vitro. A single factor experiments were conducted to compare the effects of seven additives, including brown sugar and corn meal, on the content of M. eohespera extracellular polysaccharide during liquid fermentation. On this basis, response surface methodology was used to optimize the liquid fermentation medium formula of M. eohespera exopolysaccharides. The hypoglycemic activities of extracellular polysaccharide from M. eohespera on α-amylase and α-glucosidase were determined in vitro. The results showed that when the amounts of addition of brown sugar was 2.20 g/L, urea was 3.18 g/L and corn flour was 2.40 g/L, the actual content of extracellular polysaccharide of M. eohespera could reach 1.20 g/L. At the concentration of 1.0 mg/mL, effects of extracellular polysaccharide of M. eohespera on α-glucosidase and α-amylase showed that the inhibition rates for these enzymes were 73.46% and 36.37%, respectively. The results showed that extracellular polysaccharide of M. eohespera was with hypoglycemic activity.
- Morchella eohespera,
- extracellular polysaccharides,
- medium optimization,
- response surface methodology,
- hypoglycemia

FullText(HTML)

References (33)

References

[1]	ZHANG T, ZHAO W, XIE B, et al. Effects of Auricularia auricula and its polysaccharide on diet-induced hyperlipidemia rats by modulating gut microbiota[J]. Journal of Functional Foods,2020,72:104038. doi: 10.1016/j.jff.2020.104038
[2]	XIONG C, LI Q, CHEN C, et al. Neuroprotective effect of crude polysaccharide isolated from the fruiting bodies of Morchella importuna against H₂O₂-induced PC₁₂ cell cytotoxicity by reducing oxidative stress[J]. Biomedicine & Pharmacotherapy,2016,83:569−576.
[3]	杨芳, 王新风, 翁良, 等. 两种羊肚菌胞内多糖体外抗氧化性[J]. 食品科学,2010,31(23):76−78. [YANG F, WANG X F, FENG L, et al. Antioxidant activity of intracellular polysaccharides (IPS) from Morchella esculenta L J]. Food Science,2010,31(23):76−78.
[4]	张强, 王松华, 孙玉军, 等. 羊肚菌蛋白的硒化修饰及其体外抗氧化活性[J]. 精细化工,2017,34(11):1252−1259. [ZHANG Q, WANG S H, SUN Y J, et al. Selenylation modification of Morchella esculenta proteins and their antioxidant activity in vitro[J]. Fine Chemicals,2017,34(11):1252−1259. doi: 10.13550/j.jxhg.2017.11.009
[5]	罗果, 保玉心, 李三华, 等. 羊肚菌发酵粗提产物的抗氧化活性鉴定[J]. 生物化工,2018,4(2):42−45. [LUO G, BAO Y X, LI S H, et al. The antioxidant activity of crude extraction from fermented Morchella esculenta[J]. Biochem Technol,2018,4(2):42−45. doi: 10.3969/j.issn.2096-0387.2018.02.011
[6]	王峰, 陶明煊, 程光宇, 等. 4种食用菌提取物自由基清除作用及降血糖作用的研究[J]. 食品科学,2009,30(21):343−347. [WANG F, TAO M X, CHENG G Y, et al. Free radical scavenging activities and hypoglycemic effects of aqueous extracts of four species of edible fungi[J]. Food Science,2009,30(21):343−347. doi: 10.3321/j.issn:1002-6630.2009.21.080
[7]	明建, 曾凯芳, 赵国华, 等. 羊肚菌水溶性多糖PMEP-1降血脂作用研究[J]. 食品科学,2009,30(17):285−288. [MING J, TAO M X, ZHAO G H, et al. Hypolipidemic activity of water soluble polysaccharide pmep-1 from Morchella esculenta (L.) pers[J]. Food Science,2009,30(17):285−288. doi: 10.3321/j.issn:1002-6630.2009.17.066
[8]	刘华晶, 赵妍. 羊肚菌液体培养基最适碳源氮源优化研究[J]. 南方农业,2019,13(26):136−137,145. [LIU H J, ZHAO Y. Optimization of carbon and nitrogen sources for Morchella liquid culture medium[J]. Southern Agriculture,2019,13(26):136−137,145.
[9]	刘梅森, 陈海晏, 孙红斌. 液态发酵技术培养食用菌的研究概况[J]. 江西科学,1997(3):193−198. [LIU M S, CHEN H Y, SUN H B. A study of studies on edible fungi by submerged culture technology[J]. Jiangxi Science,1997(3):193−198.
[10]	吴宽, 米伟丽, 吴云锋. 云芝糖肽发酵条件的响应面法优化试验[J]. 陕西农业科学,2020,66(1):55−58. [WU K, MI W L, WU Y F. Optimization of saccharopeptide fermentation conditions of Ganoderma formosa by response surface methodology[J]. Shanxi Agricultural Sciences,2020,66(1):55−58. doi: 10.3969/j.issn.0488-5368.2020.01.014
[11]	冮洁, 盖萌. 羊肚菌菌丝体液体培养产胞外多糖条件的研究[J]. 食用菌,2010,32(3):11−13. [GANG J, GAI M. Study on extracellular polysaccharide production conditions of Morchella mycelium by liquid culture[J]. Edible Fungi,2010,32(3):11−13. doi: 10.3969/j.issn.1000-8357.2010.03.007
[12]	杨恒, 赵萍, 刘裕慧, 等. 响应面优化发酵磨盘柿褐变条件[J]. 食品工业科技,2019,40(22):187−191. [YANG H, ZHAO P, LIU Y H, et al. Optimization of browning condition of fermented mopan persimmon by response surface methodology[J]. Science and Technology of Food Industry,2019,40(22):187−191.
[13]	DUBOIS M, GILLES K, HAMILTON J K, et al. A colorimetric method for the determination of sugars[J]. Nature,1951,168(4265):167.
[14]	赵萍, 曹心张, 张新国, 等. 冬虫夏草菌丝体发酵液中多糖的抗氧化活性研究[J]. 中医药学报,2014,42(5):27−29. [ZHAO P, CAO X Z, ZHANG X G, et al. Antioxidant activity of polysaccharide in fermentation broth of Cordyceps sinensis[J]. Acta Chinese Medicine and Pharmacology,2014,42(5):27−29. doi: 10.3969/j.issn.1002-2392.2014.05.010
[15]	王珍珍, 官月, 刘洋, 等. 六妹羊肚菌多糖的提取工艺优化及结构表征和抗氧化活性[J]. 菌物学报,2019,38(9):1548−1558. [WANG Z Z, GUAN Y, LIU Y, et al. Extraction process optimization, structural characterization and antioxidant activities of polysaccharide from Morchella sextelata[J]. Mycosystema,2019,38(9):1548−1558. doi: 10.13346/j.mycosystema.190067
[16]	刘璐, 兰阿峰, 郭素芬, 等. 羊肚菌胞外多糖提取工艺研究[J]. 生物技术,2019,29(4):382−386. [LIU L, LAN A F, GUO S F, et al. Study on the extraction technology of extracellular polysaccharide from morel[J]. Biotechnology,2019,29(4):382−386.
[17]	罗倩, 邹荣灿, 刘明月, 等. 羊肚菌多糖的提取分离纯化及保健功效研究进展[J]. 食品研究与开发,2019,40(15):211−216. [LUO Q, ZOU C R, LIU M Y, et al. Research progress of extraction, isolation, purification and health benefits of polysaccharides in Morchella[J]. Food Research and Development,2019,40(15):211−216. doi: 10.12161/j.issn.1005-6521.2019.15.035
[18]	LU X, BRENNAN M A, NARCISO J, et al. Correlations between the phenolic and fibre composition of mushrooms and the glycaemic and textural characteristics of mushroom enriched extruded products[J]. LWT,2020,118:108730. doi: 10.1016/j.lwt.2019.108730
[19]	陈树俊, 崔云. 甘薯渣多糖提取, 结构鉴定及体外功能研究[J]. 中国粮油学报,2021(9):67−73. [CHEN S J, CUI Y. Extraction, structure identification and in vitro function study of polysaccharides from sweet potato residue[J]. Journal of the Chinese Cereals and Oils Society,2021(9):67−73. doi: 10.3969/j.issn.1003-0174.2021.09.012
[20]	殷伟伟, 张松, 吴金凤. 尖顶羊肚菌活性提取物降血脂作用的研究[J]. 菌物学报,2009,28(6):873−877. [YIN W W, ZHANG S, WU J F. Hypolipidemic effect of the bioactive extract from Morchella conica[J]. Mycosystema,2009,28(6):873−877.
[21]	李井雷, 刘玉婷, 宗帅, 等. 羊肚菌胞外多糖体外降血糖降血脂活性研究[J]. 食品研究与开发,2020,41(16):39−45. [LI J L, LIU Y T, ZONG S, et al. In vitro hyperglycemic and hypolipidemic activity of Morchella esculenta extracellular polysaccharides[J]. Food Research and Development,2020,41(16):39−45. doi: 10.12161/j.issn.1005-6521.2020.16.006
[22]	YUHAN D, YANRAN Q, MIN L, et al. Antioxidant, anti-hyperlipidemia and hepatic protection of enzyme-assisted Morehella esculenta polysaccharide[J]. International Journal of Biological Macromolecules,2018:120.
[23]	ZHONG Q, ZHOU T, QIU W, et al. Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida[J]. Food Chemistry,2021,341:128148. doi: 10.1016/j.foodchem.2020.128148
[24]	LIU Y, SUN J, LUO Z, et al. Chemical composition of five wild edible mushrooms collected from Southwest China and their antihyperglycemic and antioxidant activity[J]. Food and Chemical Toxicology,2012,50(5):1238−1244. doi: 10.1016/j.fct.2012.01.023
[25]	金朝霞, 王云龙, 王培忠, 等. 羊肚菌液体发酵培养条件优化[J]. 大连工业大学学报,2014(6):416−419. [JING Z X, WANG Y L, WANG P Z, et al. Optimization of liquid fermentation conditions of Morchella[J]. Journal of Dalian Polytechnic University,2014(6):416−419. doi: 10.19670/j.cnki.dlgydxxb.2014.06.007
[26]	LI Y, YUAN Y, LEI L, et al. Carboxymethylation of polysaccharide from Morchella angusticepes peck enhances its cholesterol-lowering activity in rats[J]. Carbohydrate Polymers,2017,172:85−92. doi: 10.1016/j.carbpol.2017.05.033
[27]	CHEN J, LI L, ZHANG X, et al. Structural characterization of polysaccharide from Centipeda minima and its hypoglycemic activity through alleviating insulin resistance of hepatic HepG₂ cells[J]. Journal of Functional Foods,2021,82:104478. doi: 10.1016/j.jff.2021.104478
[28]	WU M, LI W, ZHANG Y, et al. Structure characteristics, hypoglycemic and immunomodulatory activities of pectic polysaccharides from Rosa setate×Rosa rugosa waste[J]. Carbohydrate Polymers,2021,253:117190. doi: 10.1016/j.carbpol.2020.117190
[29]	TAKEWAKI F, NAKAJIMA H, TAKEWAKI D, et al. Habitual dietary intake affects the altered pattern of cut microbiome by acarbose in patients with type 2 diabetes[J]. Nutrients,2021,13(6):2107. doi: 10.3390/nu13062107
[30]	NSARI P, FLATT P R, HARRIOTT P, et al. Insulinotropic and antidiabetic properties of eucalyptus citriodora leaves and isolation of bioactive phytomolecules[J]. Journal of Pharmacy and Pharmacology,2021,73(8):1−13.
[31]	YE L H, SUN H, ZHANG X, et al. A novel polysaccharide from Pleurotus citrinopileatus mycelia: Structural characterization, hypoglycemic activity and mechanism[J]. Food Bioscience,2020,37:100735. doi: 10.1016/j.fbio.2020.100735
[32]	王梦雅, 赵喆禛, 薛娇, 等. 桦褐孔菌纯化多糖体外降血糖活性研究[J]. 食品工业科技,2020,41(10):6. [WANG M Y, ZHAO Z Z, XUE J, et al. Hypoglycemic activity of purified polysaccharides from Inonotus obliquus in vitro[J]. Science and Technology of Food Industry,2020,41(10):6.
[33]	柏秋月, 邓百万, 杨学英, 等. 4株羊肚菌胞外多糖含量及其生物活性的研究[J]. 食品研究与开发,2020,41(8):25−31. [BAI Q Y, DENG B W, YANG X Y, et al. Study on extracellular polysaccharide content and biological activity of four Morchella strains[J]. Food Research and Development,2020,41(8):25−31. doi: 10.12161/j.issn.1005-6521.2020.08.004

Supplements (0)

Cited By

Cited by

Periodical cited type(7)

1.	左子珍，王海波，柴志欣，符健慧，张翔飞，罗晓林，钟金城. 过瘤胃蛋氨酸对牦牛半腱肌肉品质、挥发性风味物质及脂肪酸组成的影响. 畜牧兽医学报. 2024(03): 1102-1114 .
2.	姚伟琴，员丽娟，任小雨，申雯轩，徐舒婕，巩志国. 进口红花籽油氧化过程中挥发性成分变化分析. 化学试剂. 2024(08): 91-98 .
3.	王丽，张耀，刘光宪，李雪，黄锦卿，程文龙，董博，刘彩玲，何雪平，张晓. 顶空固相微萃取-气相色谱-质谱法分析桂花板鸭加工过程中挥发性风味物质的变化. 食品安全质量检测学报. 2024(19): 111-121 .
4.	王晓雨，徐恒，杨丽，张恒，王金铎，刘伟，赵旭东，王艺晓，裴龙英. 五种干腌火腿的理化特性及品质对比分析. 中国调味品. 2024(12): 73-78 .
5.	王琪，高厚基，瞿静，谢政泽，吴春霞，陈韬. 云南黑松露脂肪酶酶学性质研究及应用. 中国调味品. 2023(08): 33-38+44 .
6.	刁小琴，王莹，贾瑞鑫，孙薇婷，刘登勇，关海宁. 动物性脂肪对肉品风味影响机制研究进展. 肉类研究. 2022(03): 45-51 .
7.	王琪，瞿静，刘琨毅，黄元相，陈韬. 黑松露不同预处理温度对中式香肠品质的影响. 食品安全质量检测学报. 2022(06): 1942-1950 .