Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS

QIN Zhiwei; YE Bo; LIU Ling

doi:10.13386/j.issn1002-0306.2021120176

Volume 43 Issue 19

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(19): 340-348. > DOI: 10.13386/j.issn1002-0306.2021120176

QIN Zhiwei, YE Bo, LIU Ling. Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS[J]. Science and Technology of Food Industry, 2022, 43(19): 340−348. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120176.

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Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS

QIN Zhiwei^1,,
YE Bo^{1, 2},
LIU Ling^1, ,

1.
College of Food Science, Shenyang Agricultural University, Shenyang 110000, China
2.
Liaoning Modern Agricultural Engineering Center, Shenyang 110000, China

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Received Date: December 19, 2021
Available Online: August 03, 2022

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Abstract

Abstract

In this study, QuEChERS technology combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to establish a method for the simultaneous detection of acrylamide (AA), nitrosamines (NAs) and heterocyclic amines (HAAs) in thermally processed meat products. The results showed that the analytic methods of 20 amines in the three components showed good linearity (R²>0.991) in the corresponding concentration range, and the detection limit and quantification limit were 0.01~1.6 ng/g and 0.03~4.8 ng/g, respectively. The average daily recovery rate was between 66.3%~116.5%, and the intraday precision was between 0.78%~9.0%. The daily accuracy range of the 5×LOQ spiked level calculation for each amine was 3.4%~9.4%. The method was applied to the analysis of AA, NAs and HAAs in four meat products for grilling, in them 9 amines were detected with the concentration range of 0.03~31.26 ng/g.
- acrylamide,
- heterocyclic amines,
- nitrosamines,
- thermally processed meat products

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