Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS

QIN Zhiwei; YE Bo; LIU Ling

doi:10.13386/j.issn1002-0306.2021120176

Volume 43 Issue 19

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(19): 340-348. > DOI: 10.13386/j.issn1002-0306.2021120176

QIN Zhiwei, YE Bo, LIU Ling. Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS[J]. Science and Technology of Food Industry, 2022, 43(19): 340−348. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120176.

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Quantitative Analysis of Three Amines in Thermally Processed Meat Products Using QuEChERS Combined with UHPLC-MS/MS

QIN Zhiwei^1,,
YE Bo^{1, 2},
LIU Ling^1, ,

1.
College of Food Science, Shenyang Agricultural University, Shenyang 110000, China
2.
Liaoning Modern Agricultural Engineering Center, Shenyang 110000, China

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Received Date: December 19, 2021
Available Online: August 03, 2022

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Abstract

Abstract

In this study, QuEChERS technology combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to establish a method for the simultaneous detection of acrylamide (AA), nitrosamines (NAs) and heterocyclic amines (HAAs) in thermally processed meat products. The results showed that the analytic methods of 20 amines in the three components showed good linearity (R²>0.991) in the corresponding concentration range, and the detection limit and quantification limit were 0.01~1.6 ng/g and 0.03~4.8 ng/g, respectively. The average daily recovery rate was between 66.3%~116.5%, and the intraday precision was between 0.78%~9.0%. The daily accuracy range of the 5×LOQ spiked level calculation for each amine was 3.4%~9.4%. The method was applied to the analysis of AA, NAs and HAAs in four meat products for grilling, in them 9 amines were detected with the concentration range of 0.03~31.26 ng/g.
- acrylamide,
- heterocyclic amines,
- nitrosamines,
- thermally processed meat products

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[1]	LUND M N, RAY C A. Control of Maillard reactions in foods: Strategies and chemical mechanisms[J]. Journal of Agricultural and Food Chemistry,2017,65(23):4537−4552. doi: 10.1021/acs.jafc.7b00882
[2]	OZ F, KABAN G, KAYA M. Effects of cooking methods and levels on formation of heterocyclic aromatic amines in chicken and fish with oasis extraction method[J]. LWT-Food Science and Technology,2010,43(9):1345−1350. doi: 10.1016/j.lwt.2010.04.014
[3]	杨旭卉, 黄运安, 关天琪, 等. 红肉及其加工制品的营养价值及癌症风险控制的研究进展[J]. 现代食品,2021(8):33−37. [YANG X H, HUANG Y A, GUAN T Q, et al. Research progress on nutritional value and cancer risk control of red meat and its processed products[J]. Modern Food,2021(8):33−37. doi: 10.16736/j.cnki.cn41-1434/ts.2021.08.011 YANG X H, HUANG Y A, GUAN T Q, et al. Research progress on nutritional value and cancer risk control of red meat and its processed products[J]. Modern Food, 2021(8): 33-37. doi: 10.16736/j.cnki.cn41-1434/ts.2021.08.011
[4]	杨光, 李博, 李岳桦. QuEChERS-气相色谱-串联质谱法测定香肠和火腿肠制品中13种N-亚硝胺化合物[J]. 食品安全质量检测学报,2019,10(24):8436−8443. [YANG G, LI B, LI Y H. Determination of 13 N-nitrosamines in sausage and ham products by QuEChERS-gas chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality,2019,10(24):8436−8443. doi: 10.19812/j.cnki.jfsq11-5956/ts.2019.24.041 YANG G, LI B, LI Y H. Determination of 13 N-nitrosamines in sausage and ham products by QuEChERS-gas chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality, 2019, 10(24): 8436-8443. doi: 10.19812/j.cnki.jfsq11-5956/ts.2019.24.041
[5]	DUDA-CHODAK A, TARKO T, SROKA P, et al. A review of the interactions between acrylamide, microorganisms and food components[J]. Food Function,2016,3:1282−1295.
[6]	BIESALSKI H K. Meat as a component of a healthy diet-are there any risks or benefits if meat is avoided in the diet[J]. Meat Science,2005,70(3):509−524. doi: 10.1016/j.meatsci.2004.07.017
[7]	OZ F, KABAN G, KAYA M. Effects of cooking methods on the formation of heterocyclic aromatic amines of two different species of trout[J]. Food Chemistry,2007,10:67−72.
[8]	MAAN A A, ANJUM M A, KASHIF M, et al. Acrylamide formation and different mitigation strategies during food processing-A review[J]. Food Reviews International,2022,38:78−80.
[9]	MOLLAKHALILI-MEYBODI N, KHORSHIDIAN N, NEMATOLLAHI A, et al. Acrylamide in bread: A review on formation, health risk assessment, and determination by analytical techniques[J]. Environmental Science and Pollution Research,2021,28(13):15627−15645. doi: 10.1007/s11356-021-12775-3
[10]	QIN L, ZHANG Y Y, XU X B, et al. Isotope dilution HPLC-MS/MS for simultaneous quantification of acrylamideand 5-hydroxymethylfurfural (HMF) in thermally processed seafood[J]. Food Chemistry,2017,232:633−638. doi: 10.1016/j.foodchem.2017.04.069
[11]	JAMALI M A, ZHANG Y W, TENG H, et al. Inhibitory effectof Rosa rugosa tea extract on the formation of heterocyclic amines in meat patties at different temperatures[J]. Molecules,2016,21:173−187. doi: 10.3390/molecules21020173
[12]	SUN S Y, YUN F, YONG M X. A facile detection of acrylamide in starchy food by using a solid extraction-GC strategy[J]. Food Control,2012,26(2):220−222. doi: 10.1016/j.foodcont.2012.01.028
[13]	ZOKAEI M, ABEDI A, KAMANKESH M, et al. Ultrasonic-assisted extraction and dispersive liquid-liquid microextraction combined with gas chromatography-mass spectrometry as an efficient and sensitive method for determining of acrylamide in potato chips samples[J]. Food Chemistry,2017,234:55−61. doi: 10.1016/j.foodchem.2017.04.141
[14]	AEENEHVAND S, TOUDEHROUSTA Z, KAMANKESH M. Evaluation and application of microwave-assisted extraction and dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for the determination of polar heterocyclic aromatic amines in hamburger patties[J]. Food Chemistry,2016,190:429−435. doi: 10.1016/j.foodchem.2015.05.103
[15]	AMAYREH M, CHANBASHA B, ALHOOSHANI K, et al. Determination of N-nitrosamines by automated dispersive liquid-liquid microextraction integrated with gas chromatography and mass spectrometry[J]. Journal of Separation Science,2015,38(10):1741−1748. doi: 10.1002/jssc.201401043
[16]	IAMMARINO M, MICHELE M, CHIARAVALLE A E. Anion exchange polymeric sorbent coupled to high-performance liquid chromatography with UV diode array detection for the determination of ten N-nitrosamines in meat products: A validated approach[J]. International Journal of Food Science and Technology,2019,55(3):1097−1109.
[17]	BORTOLOMEAZZI R, ANESE M, VERARDO G, et al. Rapid mixed mode solid phase extraction method for the determination of acrylamide in roasted coffee by HPLC-MS/MS[J]. Food Chemistry,2012,135(4):2687−2693. doi: 10.1016/j.foodchem.2012.07.057
[18]	NI W, MCNAUGHTON L, LEMASTER D M, et al. Quantitation of 13 heterocyclic aromatic amines in cooked beef, pork, and chicken by liquid chromatography-electrospray ionization/tandem mass spectrometry[J]. Journal of Agricultural and Food Chemistry,2008,56(1):68−78. doi: 10.1021/jf072461a
[19]	DONG H, XIAN Y, XIAO K, et al. Development and comparison of single-step solid phase extraction and QuEChERS clean-up for the analysisof 7 mycotoxins in fruits and vegetables during storage by UHPLC-MS/MS[J]. Food Chemistry,2019,274:471−479. doi: 10.1016/j.foodchem.2018.09.035
[20]	张甜. 肉制品中N-亚硝胺检测的前处理方法及色谱条件优化[D]. 晋中: 山西农业大学, 2017. ZHANG T. Pretreatment method and optimization of chromatographic conditions for the detection of N-nitrosamines in meat products[D]. Jinzhong: Shanxi Agricultural University, 2017.
[21]	AKYÜZ M, ATA S, DINÇ E. A chemometric optimization of method for determination of nitrosamines in gastric juices by GC-MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2016,117:26−36. doi: 10.1016/j.jpba.2015.08.021
[22]	侯慧文. 不同烤制程度下羊肉中杂环胺生成规律的研究[D]. 锦州: 渤海大学, 2021. HOU H W. Study on the formation law of heterocyclic amines in mutton under different roasting degrees[D]. Jinzhou: Bohai University, 2021.
[23]	ALAM S, AHMAD R, PRANAW K, et al. Asparaginase conjugated magnetic nanoparticles used for reducing acrylamide formation in food model system[J]. Bioresource Technology,2018,269:121−126. doi: 10.1016/j.biortech.2018.08.095
[24]	DOURADO C, PINTO C A, CUNHA S C, et al. A novel strategy of acrylamide mitigation in fried potatoes using asparaginase and high pressure technology[J]. Innovative Food Science and Emerging Technologies,2020,60:102310. doi: 10.1016/j.ifset.2020.102310
[25]	ZUO S, ZHANG T, JIANG B. et al. Reduction of acrylamide level through blanching with treatment by an extremely thermostable L-asparaginase during French fries process[J]. Extremophiles,2015,19(4):841−851. doi: 10.1007/s00792-015-0763-0
[26]	李潇潇. 肉制品中丙烯酰胺形成规律的研究[D]. 天津: 天津科技大学, 2016. LI X X. Study on formation regularity of acrylamide in meat products[D]. Tianjin: Tianjin University of Science and Technology, 2016.
[27]	DONG H, ZENG X F, BAI W D. Solid phase extraction with high polarity Carb/PSA as composite fillers prior to UPLC-MS/MS to determine six bisphenols and alkylphenols in trace level hotpot seasoning[J]. Food Chemistry,2018,258:206−213. doi: 10.1016/j.foodchem.2018.03.074
[28]	FLORES M, MORA L, REIG M, et al. Risk assessment of chemical substances of safety concern generated in processed meats[J]. Food Science and Human Wellness,2019,8(3):244−251. doi: 10.1016/j.fshw.2019.07.003
[29]	朱清清. 腊肉加工过程中亚硝胺生成规律及其控制研究[D]. 天津: 天津科技大学, 2015. ZHU Q Q. Research on the formation law and control of nitrosamines in the processing of bacon[D]. Tianjin: Tianjin University of Science and Technology, 2015.
[30]	HASKARACA G, DEMIROK SONCU E, KOLSARICI N, et al. Heterocyclic aromatic amines content in chicken burgers and chicken nuggets sold in fast food restaurants and effects of green tea extract and microwave thawing on their formation[J]. Journal of Food Processing and Preservation,2017,41(6):e13240. doi: 10.1111/jfpp.13240
[31]	GU Y S, KIM I S, AHN J K. Mutagenic and carcinogenic heterocyclic amines as affected by muscle types/skin and cooking in pan roasted mackerel[J]. Mutatation Research,2020,515(1−2):189−195.

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