Optimization of Process of Hydrolysis of <i>Dendrobium officinale</i> Polysaccharide and Determination of Contents of Its Mannose

TAN Shancai; TAN Changjin; WU Xiaolan; YANG Yang; LIU Hongyan

doi:10.13386/j.issn1002-0306.2021120032

Volume 43 Issue 19

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(19): 220-227. > DOI: 10.13386/j.issn1002-0306.2021120032

TAN Shancai, TAN Changjin, WU Xiaolan, et al. Optimization of Process of Hydrolysis of Dendrobium officinale Polysaccharide and Determination of Contents of Its Mannose[J]. Science and Technology of Food Industry, 2022, 43(19): 220−227. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120032.

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Optimization of Process of Hydrolysis of Dendrobium officinale Polysaccharide and Determination of Contents of Its Mannose

1.
Tongren Polytechnic College, Tongren 554300, China
2.
Key Open Laboratory of Traditional Chinese Veterinnary Medicine, Tongren 554300, China
3.
Xinjiang Medical University, Urumqi 830000, China

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Received Date: December 05, 2021
Available Online: August 02, 2022

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Abstract

Abstract

Process of hydrolysis of Dendrobium officinale polysaccharide by high temperature and pressure-sulfuric acid assisted was optimized by response surface method (RSM) according to Central Composite Design (CCD), and polysaccharides of Dendrobium officinale under the conditions of different cultivation were determined by phenol-sulfuric acid method, and the contents of mannose from different parts (stems, leaves and flowers) of Dendrobium officinale were analyzed by pre-column derivation HPLC method. The research showed that the optimum parameters was as follow: Material-liquid ratio was 1:1.5 (mg/mL), concentrations of sulfuric acid was 1 mol/L, hydrolysis temperature was 112 ℃ (0.15 MPa) and hydrolysis time was 44 min. Under optimal conditions, the content of the mannose was 64.35%±0.76% with stem as the test material. The contents of polysaccharide in stems, leaves and flowers of Dendrobium officinale under the conditions of same cultivation were significantly differences (P<0.01, stems>leaves>flowers). And the contents of mannose in the samples was the same as that of polysaccharide. The content of polysaccharide and mannose in the stems were 29.32%~33.78% or 18.17%~21.05%, respectively. And the contents of polysaccharide and mannose in the leaves were 15.91%~17.31% or 10.28%~11.11%, respectively. And the contents of polysaccharide and mannose in the flowers were 8.37%~8.68% or 5.30%~5.75%, respectively. The results indicated that the method was effective and efficient, which could be used for the hydrolysis of Dendrobium officinale polysaccharide by high temperature and pressure-sulfuric acid assisted. And the stems, leaves and flowers of Dendrobium officinale were high-quality natural resources for the preparation of mannose.
- Dendrobium officinale,
- polysaccharide,
- hydrolysis,
- response surface method,
- mannose

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