Protective Effect of 6′′′-Feruloylspinosin on A<i>β</i><sub>1-42</sub>-Induced SH-SY5Y Cells Injury

ZHANG Mei; ZHANG Yanqing; XIE Junbo; WANG Qing; QI Wuqin

doi:10.13386/j.issn1002-0306.2021120012

Volume 43 Issue 13

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(13): 373-380. > DOI: 10.13386/j.issn1002-0306.2021120012

ZHANG Mei, ZHANG Yanqing, XIE Junbo, et al. Protective Effect of 6′′′-Feruloylspinosin on Aβ_1-42-Induced SH-SY5Y Cells Injury[J]. Science and Technology of Food Industry, 2022, 43(13): 373−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120012.

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Protective Effect of 6′′′-Feruloylspinosin on Aβ_1-42-Induced SH-SY5Y Cells Injury

1.
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
2.
College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China

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Received Date: December 01, 2021
Available Online: April 29, 2022

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Abstract

Abstract

Objective: To investigate the protective effects of 6′′′-feruloylspinosinon Aβ_1-42-induced SH-SY5Y cells injury. Methods: SH-SY5Y cells were pretreated with 6′′′-feruloylspinosin (1、5、10、20 and 40 μmol/L) for 2 h and then co-treated with 5 μmol/L of Aβ_1-42 for 24 h. Cell viability was measured by CCK-8 assay; cell morphology was observed by Calcein-AM/PI assay; intracellular reactive oxygen species, malonic dialdehyde level, Glutathione peroxidaseactivity and mitochondrial membrane potential level were detected by the kit; expression of apoptosis-related proteins: Bcl-xL/Bcl-2 associated death promoter, B-cell lymphoma/leukemia-2 were detected by Western Blotting. Results: 6′′′-feruloylspinosin could inhibit Aβ_1-42-induced apoptosis and improve cell viability (P<0.001); reduce intracellular reactive oxygen species (P<0.001) and malondialdehyde (P<0.01) levels; increase glutathione peroxidase activity (P<0.001); upregulate cell mitochondrial membrane potential level (P<0.001); regulate apoptosis-related protein expression (P<0.01). Conclusion: The results validated that 6′′′-feruloylspinosin had a protective effect on SH-SY5Y cells with Aβ_1-42-induced damage, and the mechanism was that 6′′′-feruloylspinosin could alleviate oxidative damage and regulate the expression of apoptosis related proteins.
- Ziziphi Spinosae Semen,
- 6′′′-feruloylspinosin,
- amyloid β,
- antioxidant activity,
- mitochondria

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