The Effect of Polysaccharide of <i>Poria cocos</i> on Key Metabolites of <i>Bifidobacterium</i> BB-12

ZHANG Tianyang; ZHANG Xuyan; WANG Danping; XU Xiaoxi

doi:10.13386/j.issn1002-0306.2021110200

Volume 43 Issue 11

May 2022

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Science and Technology of Food Industry > 2022 > 43(11): 24-33. > DOI: 10.13386/j.issn1002-0306.2021110200

ZHANG Tianyang, ZHANG Xuyan, WANG Danping, et al. The Effect of Polysaccharide of Poria cocos on Key Metabolites of Bifidobacterium BB-12[J]. Science and Technology of Food Industry, 2022, 43(11): 24−33. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110200.

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The Effect of Polysaccharide of Poria cocos on Key Metabolites of Bifidobacterium BB-12

ZHANG Tianyang^{1, 2,},
ZHANG Xuyan^{1, 2},
WANG Danping^{1, 2},
XU Xiaoxi^{1, 2, ,}

1.
College of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Key Laboratory of Dairy Science, Ministry of Education, Harbin 150030, China

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Received Date: November 16, 2021
Available Online: April 16, 2022

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Abstract

Abstract

In order to explore the key metabolites of Bifidobacterium animalis spp. lactis BB-12 metabolizing polysaccharides of Poria cocos, a non-targeted metabolomics method based on liquid chromatography-mass spectrometry (LC-MS/MS) was used, and glucose was used as a control. The effects of polysaccharides of Poria cocos on key metabolites of Bifidobacterium animalis spp. lactis BB-12 were analyzed. The results showed that polysaccharides of Poria cocos could significantly promote the growth of Bifidobacterium BB-12. By using component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), some representative differential metabolites were screened out such as palmatine and ganolucidic acid, etc. There were 12 kinds of key differential metabolites, and 17 differential metabolic pathways were significantly enriched such as ABC transporters, linoleic acid metabolism, and galactose metabolism. These metabolites and pathways demonstrated that Bifidobacterium BB-12 exhibited better growth performance and prebiotic efficacy in the case of fermenting polysaccharides of Poria cocos. This experiment analyzed the metabolites produced by polysaccharides of Poria cocos from the level of small molecular metabolites which were metabolized by Bifidobacterium animalis spp. lactis BB-12. The results provide the theoretical basis and guidance for further study on the probiotic effect of polysaccharides of Poria cocos and Bifidobacterium animalis spp. lactis BB-12 in human bodies.
- polysaccharides of Poria cocos,
- prebiotics,
- Bifidobacterium BB-12,
- non-targeted metabolomics,
- key metabolites

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