Activity and Mechanism of <i>Citri grandis </i>Exocarpium-<i>Cordyceps militaris</i> Compound on Reliving Cough Based on Network Pharmacology

JIAO Chunwei; ZHONG Jing; HUANG Haiyong; LIANG Huijia; ZHENG Jiaoji; CHEN Jiaming; XIE Yizhen

doi:10.13386/j.issn1002-0306.2021100261

Volume 43 Issue 13

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(13): 17-24. > DOI: 10.13386/j.issn1002-0306.2021100261

JIAO Chunwei, ZHONG Jing, HUANG Haiyong, et al. Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology[J]. Science and Technology of Food Industry, 2022, 43(13): 17−24. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100261.

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Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology

1.
Guangdong Yuewei Biotechnology Co., Ltd., Zhaoqing 512000, China
2.
Guangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, China
3.
Guangdong Nan Yue Pharmaceutical Co., Ltd., Guangzhou 510530, China

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Received Date: October 25, 2021
Available Online: May 20, 2022

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Abstract

Abstract

This study aimed to investigate the activity and mechanism of Citri grandis Exocarpium-Cordyceps militaris compound on reliving cough by the use of network pharmacology. Mouse cough inducing models were constructed by ammonia water to evaluate the cough suppressant activity of the compound. Multiple online databases and literature searches were then used to collect the active ingredients, targets of action and disease targets of the compound. Construction of compound-target-disease networks and protein interaction networks using Cytoscape and STRING, and GO and KEGG enrichment analysis of target genes using Meatscape. The results showed that the low and high dose groups significantly reduced the number of coughs in mice (P<0.001) and decreased the levels of three inflammatory factors, tumor necrosis factor, interleukin 1α and interleukin 1β in mice serum, with significant differences in the high dose group (P<0.05, P<0.01, P<0.05). The results of the network pharmacological analysis showed that this compound could act on 78 targets including transcription factor AP-1, interleukin 6 and tumor necrosis factor, regulating 575 GO entries and 272 signalling pathways to exert cough suppressant effects, of which cordycepic acid, cordycepin, apigenin, beta-sitosterol and naringenin were the important material basis. The results indicated that the combination of Citri grandis Exocarpium-Cordyceps militaris has cough suppressant activity, which was the result of the combined action of multiple substances in the combination on multiple targets and pathways.
- Citri grandis Exocarpium,
- Cordyceps militaris,
- cough,
- network pharmacology

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References

[1]	PLEVKOVA J, BROZMANOVA M, MATLOOBI A, et al. Animal models of cough[J]. Respiratory Physiology & Neurobiology,2021,290:103656.
[2]	高龙霞, 阎玥, 包海鹏, 等. 咳嗽变异性哮喘现代研究进展[J]. 中华中医药杂志,2021,34(9):4171−4174. [GAO L X, YAN Y, BAO H P, et al. Modern research progress of cough variant asthma[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2021,34(9):4171−4174. GAO L X, YAN Y, BAO H P, et al. Modern research progress of cough variant asthma[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2021, 34(9): 4171-4174.
[3]	王艳慧. 化橘红的研究进展[J]. 世界科学技术-中医药现代化,2017,19(6):1076−1082. [WANG Y H. Research progress of Citri grandis Exocarpium[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2017,19(6):1076−1082. WANG Y H. Research Progress of Citri grandis Exocarpium[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2017, 19(6): 1076-1082.
[4]	CHEN Y, WU Y, LI S, et al. Large-scale isolation and antitumor mechanism evaluation of compounds from the traditional Chinese medicine Cordyceps militaris[J]. European Journal of Medicinal Chemistry,2021,212:113142. doi: 10.1016/j.ejmech.2020.113142
[5]	嵇冰. 平喘固本汤治疗稳定期慢性阻塞性肺病的临床研究[D]. 苏州: 苏州大学, 2012. JI B. Clinical study of Pingchuanguben Tang on stable chronic obstructive pulmonary disease (COPD)[D]. Suzhou: Soochow University, 2012.
[6]	杜雨茂. 痰饮咳嗽(慢性支气管炎并间质性感染)一例治验[J]. 陕西中医学院学报,1978(2):23−25. [DU Y M. A case of cough due to phlegm retention (Bronchitis and Interstitial Infection)[J]. Journal of Shaanxi College of Traditional Chinese Medicine,1978(2):23−25. DU Y M. A case of cough due to phlegm retention (Bronchitis and Interstitial Infection)[J]. Journal of Shaanxi College of Traditional Chinese Medicine, 1978(2): 23-25.
[7]	毛树贵. 浅谈天降镇压胶囊治高血压与天降镇咳胶囊的独特功效[J]. 中华养生保健,2004(10):50. [MAO S G. Discussion on the unique efficacy of Tianjiang Zhenke capsule and Tianjiang Zhenke capsule in treating hypertension[J]. China Health Care,2004(10):50. MAO S G. Discussion on the unique efficacy of Tianjiang Zhenke capsule and Tianjiang Zhenke capsule in treating hypertension[J]. China Health Care, 2004(10) : 50.
[8]	陈志华. 橘红痰咳液治疗小儿急性支气管炎的临床疗效分析[J]. 中国处方药,2019,17(1):86−87. [CHEN Z C. Clinical analysis of Juhong Tanke liquid in treating acute bronchitis in children[J]. Journal of China Prescription Drug,2019,17(1):86−87. doi: 10.3969/j.issn.1671-945X.2019.01.056 CHEN Z C. Clinical analysis of Juhong Tanke liquid in treating acute bronchitis in children[J]. Journal of China Prescription Drug, 2019, 17(1) : 86-87. doi: 10.3969/j.issn.1671-945X.2019.01.056
[9]	孟泽彬, 陈林会, 韩近雨, 等. 蛹虫草化学活性成分的研究进展[J]. 分子植物育种,2015,13(9):2147−2154. [MENG Z M, CEHN L H, HAN J Y, et al. Research advances of the chemical active constituents of Cordyceps militaris[J]. Molecular Plant Breeding,2015,13(9):2147−2154. MENG Z M, CEHN L H , HAN J Y, et al. Research advances of the chemical active constituents of Cordyceps militaris[J]. Molecular Plant Breeding, 2015, 13(9) : 2147-2154.
[10]	谢伟娜, 王宝娟, 张海波, 等. 肺炎颗粒成型工艺的优化及其对小鼠的镇咳作用[J]. 中成药,2021,43(5):1123−1128. [XIE W N, WANG B J, SHANG H B, et al. Optimization of forming process for granules for pneumonia and its antitussive effect on mice[J]. Chinese Traditional Patent Medicine,2021,43(5):1123−1128. doi: 10.3969/j.issn.1001-1528.2021.05.002 XIE W N, WANG B J, SHANG H B, et al. Optimization of forming process for granules for pneumonia and its antitussive effect on mice[J]. Chinese Traditional Patent Medicine, 2021, 43(5): 1123-1128. doi: 10.3969/j.issn.1001-1528.2021.05.002
[11]	王翰华, 杨晓春, 陈云. 宁海白枇杷花醇提物不同极性部位的止咳化痰抗炎活性[J]. 中国老年学杂志,2019,39(6):1431−1434. [WANG H H, YANG X C, CHEN Y. Antitussive, expectorant and anti-inflammatory activities of ethanol extracts from the flower of Eriobotrya japonica in Ninghai County[J]. Chinese Journal of Gerontology,2019,39(6):1431−1434. doi: 10.3969/j.issn.1005-9202.2019.06.048 WANG H H, YANG X C, CHEN Y. Antitussive, expectorant and anti-inflammatory activities of ethanol extracts from the flower of Eriobotrya japonica in Ninghai County[J]. Chinese Journal of Gerontology, 2019, 39(6) : 1431-1434. doi: 10.3969/j.issn.1005-9202.2019.06.048
[12]	盖国忠, 金顺姬, 王波, 等. 蛹虫草菌粉胶囊和金水宝胶囊对照治疗慢性支气管炎[J]. 中国新药杂志,2004(2):169−171. [GAI G Z, JIN S J, WANG B, et al. The efficacy of Cordyceps militaris capsules in treatment of chronic bronchitis in comparison with Jinshuibao capsules[J]. Chinese New Drugs Journal,2004(2):169−171. doi: 10.3321/j.issn:1003-3734.2004.02.024 GAI G Z, JIN S J, WANG B, et al. The efficacy of Cordyceps militaris capsules in treatment of chronic bronchitis in comparison with Jinshuibao capsules[J]. Chinese New Drugs Journal, 2004(2): 169-171. doi: 10.3321/j.issn:1003-3734.2004.02.024
[13]	蔡鸿彦, 刘志成, 逄建伟, 等. 蛹虫草菌粉胶囊治疗慢性支气管炎510例[J]. 中国新药杂志,2004(2):171−174. [CAI H Y, LIU Z C, PANG J W, et al. Efficacy of Cordyceps militaris capsules in treatment of chronic bronchitis[J]. Chinese New Drugs Journal,2004(2):171−174. doi: 10.3321/j.issn:1003-3734.2004.02.025 CAI H Y, LIU Z C, PANG J W, et al. Efficacy of Cordyceps militaris capsules in treatment of chronic bronchitis[J]. Chinese New Drugs Journal, 2004(2): 171-174. doi: 10.3321/j.issn:1003-3734.2004.02.025
[14]	侯秀娟, 沈勇根, 徐明生, 等. 化州橘红多糖对小鼠消炎、止咳及化痰功效的影响研究[J]. 现代食品科技,2013,29(6):1227−1229. [HOU C J, SHEN Y G, XU M S, et al. Dephlogisticate, antitussive and expectorant effects of polysaccharides from Citri grandis Exocarpium on mice[J]. Modern Food Science and Technology,2013,29(6):1227−1229. HOU C J, SHEN Y G, XU M S, et al. Dephlogisticate, antitussive and expectorant effects of polysaccharides from Citri grandis Exocarpium on mice[J]. Modern Food Science and Technology, 2013, 29(6): 1227-1229.
[15]	CHAE H, KIM S Y, PEL P, et al. Standardized extract of Atractylodis Rhizoma Alba and Fructus Schisandrae ameliorates coughing and increases expectoration of phlegm[J]. Molecules,2020,25(13):3064. doi: 10.3390/molecules25133064
[16]	YANG E J, LEE J, SONG B B, et al. Anti-inflammatory effects of ethanolic extract from Lagerstroemia indica on airway inflammation in mice[J]. Journal of Ethnopharmacology,2011,136(3):422−427. doi: 10.1016/j.jep.2010.05.066
[17]	ZHONG S, NIE Y, GAN Z, et al. Effects of Schisandra chinensis extracts on cough and pulmonary inflammation in a cough hypersensitivity guinea pig model induced by cigarette smoke exposure[J]. Journal of Ethnopharmacology,2015,165:73−82. doi: 10.1016/j.jep.2015.02.009
[18]	王春燕, 隆红艳. 黄龙止咳口服液对咳嗽变异性哮喘模型小鼠的作用机制研究[J]. 天津中医药大学学报,2020,39(3):330−335. [WANG C W, LONG H Y. Study on the mechanism of Huanglong Zhike oral liquid on mouse with cough variant asthma model[J]. Journal of Tianjin University of Traditional Chinese Medicine,2020,39(3):330−335. YAN C W, YAN H L. Study on the mechanism of Huanglong Zhike oral liquid on mouse with cough variant asthma model[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2020, 39(3): 330-335.
[19]	王红霞, 郭烁, 郭雨晴, 等. 百杏润肺止咳方对肺阴亏虚型咳嗽变异性哮喘患者炎症因子及肺功能的影响[J]. 中国中医基础医学杂志,2019,25(10):1377−1379. [WANG H X, GUO S, GUO Y Q, et al. Effect of Baixing Runfei Zhike recipe on inflammatory factors and lung function in patients with cough variant asthma of deficiency of Lung Yin typet[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2019,25(10):1377−1379. WANG H X, GUO S, GUO Y Q, et al. Effect of Baixing Runfei Zhike recipe on inflammatory factors and lung function in patients with cough variant asthma of deficiency of lung yin typet[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine, 2019, 25(10): 1377-1379.
[20]	陈欲云, 马清萍, 王涛, 等. 柚皮素止咳化痰平喘作用的研究[J]. 食品工业科技,2014,35(19):355−358. [CHEN Y Y, MA Q P, WANG T, et al. Experimental study on antitussive, expectorant and anti-asthmatic effects of naringenin[J]. Science and Technology of Food Industry,2014,35(19):355−358. CHEN Y Y, MA Q P, WANG T, et al. Experimental study on antitussive, expectorant and anti-asthmatic effects of naringenin[J]. Science and Technology of Food Industry, 2014, 35(19): 355-358.
[21]	LUO Y, ZHANG C, LI P, et al. Naringin attenuates enhanced cough, airway hyperresponsiveness and airway inflammation in a guinea pig model of chronic bronchitis induced by cigarette smoke[J]. International Immunopharmacology,2012,13(3):301−307. doi: 10.1016/j.intimp.2012.04.019
[22]	IWAMURA C, SHINODA K, YOSHIMURA M, et al. Naringenin chalcone suppresses allergic asthma by inhibiting the type-2 function of CD4 T cells[J]. Allergol Int,2010,59(1):67−73. doi: 10.2332/allergolint.09-OA-0118
[23]	YANG J, LI Q, ZHOU X D, et al. Naringenin attenuates mucous hypersecretion by modulating reactive oxygen species production and inhibiting NF-κB activity via EGFR-PI3K-Akt/ERK MAPKinase signaling in human airway epithelial cells[J]. Molecular and Cellular Biochemistry,2011,351(1-2):29−40. doi: 10.1007/s11010-010-0708-y
[24]	FEI X, ZHANG X, ZHANG G, et al. Cordycepin inhibits airway remodeling in a rat model of chronic asthma[J]. Biomedicine & Pharmacotherapy,2017,88:335−341.
[25]	LI R, PANG L, DU Q, et al. Apigenin inhibits allergen-induced airway inflammation and switches immune response in a murine model of asthma[J]. Immunopharmacology and Immunotoxicology,2010,32(3):364−370. doi: 10.3109/08923970903420566
[26]	LAMPRONTI I, DECHECCHI M C, RIMESSI A, et al. β-Sitosterol reduces the expression of chemotactic cytokine genes in cystic fibrosis bronchial epithelial cells[J]. Frontiers in Pharmacology,2017,8:e00236.
[27]	刘晓霞. 蛋白质交互网络中的复合物识别与应用研究[D]. 大连: 大连理工大学, 2018. LIU X X. Research on identification and application of protein complexes in protein-protein interaction networks[D]. Dalian: Dalian University of Technology, 2018.
[28]	PATIL R H, NAVEEN KUMAR M, KIRAN KUMAR K M, et al. Dexamethasone inhibits inflammatory response via down regulation of AP-1 transcription factor in human lung epithelial cells[J]. Gene,2018,645:85−94. doi: 10.1016/j.gene.2017.12.024
[29]	张青, 徐剑铖, 毛宝龄, 等. 内毒素致伤大鼠肺组织TNF-α、IL-6的mRNA表达及NF-IL6活化研究[J]. 中国危重病急救医学,2001(9):523−526. [ZHANG Q, XU J C, MAO B L, et al. Studies on the expression of tumor necrosis factor-α and interleukin-6 mRNA and the activity of nuclear factor in acute lung injury induced by lipopolysaccharide[J]. Chinese Critical Care Medicine,2001(9):523−526. ZHANG Q, XU J C, MAO B L, et al. Studies on the expression of tumor necrosis factor-α and interleukin-6 mRNA and the activity of nuclear factor in acute lung injury induced by lipopolysaccharide[J]. Chinese Critical Care Medicine, 2001(9): 523-526.
[30]	SONG X, QIAN Y. IL-17 family cytokines mediated signaling in the pathogenesis of inflammatory diseases[J]. Cellular Signalling,2013,25(12):2335−2347. doi: 10.1016/j.cellsig.2013.07.021
[31]	JAROSZ-GRIFFITHS H H, HOLBROOK J, LARA-REYNA S, et al. TNF receptor signalling in autoinflammatory diseases[J]. International Immunology,2019,31(10):639−648. doi: 10.1093/intimm/dxz024
[32]	OHTSU A, SHIBUTANI Y, SENO K, et al. Advanced glycation end products and lipopolysaccharides stimulate interleukin-6 secretion via the RAGE/TLR4-NF-κB-ROS pathways and resveratrol attenuates these inflammatory responses in mouse macrophages[J]. Experimental and Therapeutic Medicine,2017,14(5):4363−4370.
[33]	YU W, TAO M, ZHAO Y, et al. 4’-Methoxyresveratrol alleviated AGE-induced inflammation via RAGE-mediated NF-κB and NLRP3 inflammasome pathway[J]. Molecules,2018,23(6):1447. doi: 10.3390/molecules23061447

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