Extraction of Melanin in Apricot Kernel Shell and the Optimization of Chelating Process of Its Metal Chelates

GAO Li; LIU Linlin; LIU Panpan; XU Hongyu; WANG Haibin; LIU Haiying; YANG Liu

doi:10.13386/j.issn1002-0306.2021100255

Volume 43 Issue 13

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(13): 225-234. > DOI: 10.13386/j.issn1002-0306.2021100255

GAO Li, LIU Linlin, LIU Panpan, et al. Extraction of Melanin in Apricot Kernel Shell and the Optimization of Chelating Process of Its Metal Chelates[J]. Science and Technology of Food Industry, 2022, 43(13): 225−234. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100255.

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Extraction of Melanin in Apricot Kernel Shell and the Optimization of Chelating Process of Its Metal Chelates

School of Chemical Engineering and Technology, North University of China, Taiyuan 030051, China

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Received Date: October 25, 2021
Available Online: April 29, 2022

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Abstract

Abstract

In this study, single factor and response surface methodology were used to optimize the extraction parameters of melanin from waste apricot kernel shell. The structure of the obtained melanin was identified by UV-vis/IR absorption spectrum and scanning electron microscope. Then, the chelating effect of extracted melanin with Fe³⁺, Cu²⁺ and Zn²⁺ metal ions were used as the index, and the single factor experiment was used to optimize the chelating process. In order to provide theoretical basis for further development and utilization of the chelate as functional food, the structure of the chelate was identified by UV-vis absorption spectrum, infrared absorption spectrum, scanning electron microscope and energy spectrum scanning. The results showed that the order of influence of the selected factors on the yield of melanin was: ultrasonic time, alkali concentration, pH, solid-liquid ratio. According to the results of response surface model, the optimal extraction conditions were as follows: solid-liquid ratio 1:10 g/mL, sodium hydroxide concentration 1.5 mol/L, acid precipitation pH value 1, ultrasonic time 35 min, under this condition, the yield was 4.78%±0.23% . The optimum chelating process of iron melanin chelate was as follows: iron ion concentration 5 mmol/L, pH5.5, reaction for 8 h; The optimum chelating conditions of copper melanin chelate was copper ion concentration 4 mmol/L, pH7.5, reaction for 6 h. The optimal chelating process of zinc melanin chelate was as follows: zinc ion concentration 3 mmol/L, pH7.5, reaction 4 h. Under the same conditions, the chelating rate of iron melanin reached 63.86%. Ultraviolet and infrared results showed that melanin chelated with metal ions mainly through amino, carbonyl or carboxyl groups. Scanning electron microscopy and energy spectrum results showed that the three metal chelates were successfully prepared. This study provided a theoretical basis for the development of functional food additives and metal element supplements and had a wide range of practical value and market prospects.

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[1]	CHU M, HAI W, ZHANG Z, et al. Melanin nanoparticles derived from a homology of medicine and food for sentinel lymph node mapping and photothermal in vivo cancer therapy[J]. Biomaterials,2016,91:182−199. doi: 10.1016/j.biomaterials.2016.03.018
[2]	DISCHIA M, WAKAMATSU K, CICOIRA F, et al. Melanins and melanogenesis: From pigment cells to human health and technological applications[J]. Pigment Cell & Melanoma Research,2015,28(5):520−544.
[3]	ELOBEID A, ALHARBI S, ALJOMAH N, et al. Herbal melanin modulates tumor necrosis factor alpha (TNF-α), interleukin 6(IL-6) and vascular endothelial growth factor (VEGF) production[J]. Phytomedicine,2006,13(5):324−333. doi: 10.1016/j.phymed.2005.03.007
[4]	DIMAURO E , CAMAGGI M, VANDOOREN N, et al. Eumelanin for nature-inspired UV-absorption enhancement of plastics. Polymer International, 2019, 68(5): 984−991.
[5]	MURIUKI E, HALL C, SASANYA J J, et al. Purification, analysis and antimicrobial activity of Fusarium graminearum pigment[J]. American Chemical Society Southeast Regional Meeting,2008,24(2):37−43.
[6]	李杰, 贾栩超, 张瑞芬, 等. 黑芝麻黑色素的分离纯化, 结构表征及体外抗氧化活性[J]. 中国农业科学,2020(12):2477−2492. [LI J, JIA Y C, ZHANG R F, et al. Isolation, purification, structure characterization and in vitro antioxidant activity of melanin from black sesame[J]. Scientia Agricultura Sinica,2020(12):2477−2492. doi: 10.3864/j.issn.0578-1752.2020.12.014 LI J, JIA Y C, ZHANG R F, et al. Isolation, purification, structure characterization and in vitro antioxidant activity of melanin from black sesame[J]. Scientia Agricultura Sinica, 2020(12): 2477-2492. doi: 10.3864/j.issn.0578-1752.2020.12.014
[7]	HUNG Y C, SAVA V M, BLAGODARSKY V A, et al. Protection of tea melanin on hydrazine-induced liver injury[J]. Life Sciences,2003,72(9):1061−1071. doi: 10.1016/S0024-3205(02)02348-2
[8]	QI C, FU L H, XU H, et al. Melanin/polydopamine-based nanomaterials for biomedical applications[J]. Science China Chemis-try,2019,62(2):162−88. doi: 10.1007/s11426-018-9392-6
[9]	郭玉华, 郁有祝, 牛永生. 木犀草素-Fe³⁺物及其自由基清除活性的研究[J]. 安阳工学院学报,2021(4):28−29. [GUO Y H, YU Y Z, NIU Y S. Studies on luteolin-Fe³⁺ complex and its free radical scavenging activity[J]. Journal of Anyang Institute of Technology,2021(4):28−29. GUO Y H, YU Y Z, NIU Y S. Studies on luteolin-Fe³⁺ complex and its free radical scavenging activity[J]. Journal of Anyang Institute of Technology, 2021(4): 28-29.
[10]	唐春丽, 李振宏, 朱志仁等. 一种罗汉果叶黄酮Cu配合物的表征及活性研究[J]. 安徽医科大学学报,2012,47(12):1427−1431. [TANG C L, LI Z H, ZHU Z R, et al. Characterization and activity of Cu complex with a flavonoid from Siraitia grosvenorii (Swingle) C. Jeffrey leaves[J]. Journal of Anhui Medical University,2012,47(12):1427−1431. TANG C L, LI Z H, ZHU Z R, et al. Characterization and activity of Cu complex with a flavonoid from Siraitia grosvenorii (Swingle) C. Jeffrey leaves[J]. Journal of Anhui Medical University, 2012, 47(12): 1427-1431.
[11]	朱湛, 王亚利, 杨艳萍, 等. 几种新透明质酸金属配合物的合成及生物活性[J]. 北京理工大学学报,2012,32(11):1200−1204. [ZHU Z, WANG Y L, YANG Y P, et al. Synthesis of new hyaluronic metal complexes and investigation of biological activity[J]. Transactions of Beijing Institute of Technology,2012,32(11):1200−1204. doi: 10.3969/j.issn.1001-0645.2012.11.022 ZHU Z, WANG Y L, YANG Y P, et al. Synthesis of New Hyaluronic Metal Complexes and Investigation of Biological Activity[J]. Transactions of Beijing Institute of Technology, 2012, 32(11): 1200-1204. doi: 10.3969/j.issn.1001-0645.2012.11.022
[12]	SHENG S, LIU Y, MING Y, et al. Antioxidant activity of a Lachnum YM226 melanin-iron complex and its influence on cytokine production in mice with iron deficiency anemia[J]. Food & Function,2016,7(3):1508−1514.
[13]	WANG F R, XIE Z G, YE X Q, et al. Effectiveness of treatment of iron deficiency anemia in rats with squid ink melanin-Fe[J]. Food & Function,2013,5(1):123−128.
[14]	MENTER, JULIAN M. Melanin from a physicochemical point of view[J]. Polymer International,2016,11(65):1300−1305.
[15]	SHANMUGANATHAN K, CHO J H, IYER P, et al. Thermooxidative stabilization of polymers using natural and synthetic melanins[J]. Macromolecules,2016,44(24):9499−9507.
[16]	JU K Y, LEE Y, LEE S, et al. Bioinspired polymerization of dopamine to generate melanin-like nanoparticles having an excellent free-radical-scavenging property[J]. Biomacromolecules,2011,12(3):625−632. doi: 10.1021/bm101281b
[17]	康静静. 黑芝麻黑色素的分离及其结构性质的研究[D]. 南昌: 南昌大学, 2015. KANG J J. Study of the extraction, purification, structural characteristics and physicochemical property of black pigment from semen Sesami nigrum[D]. Nanchang: Nanchang University, 2015.
[18]	王改利. 花椒籽种皮黑色素的提取纯化及性能研究[D]. 西安: 陕西科技大学, 2014. WANG G L. Extracting, purifying and performance of pepper seed melanin[D]. Xi'an: Shaanxi University of Science and Technology, 2014.
[19]	赵博. 出芽短梗霉黑色素提取及性质研究[D]. 天津: 天津科技大学, 2013. ZHAO B. Study on extraction and qualities of melanin from Aureobasidium pullulans[D]. Tianjin: Tianjin University of Science and Technology, 2013.
[20]	任燕玲, 杨柳, 高莉, 等. 黑色素金属螯合物的研究进展及应用[J]. 材料导报,2020,34(11):145−152. [REN Y L, YANG L, GAO L, et al. Research progress and application of melanin metal chelate[J]. Materials Reports,2020,34(11):145−152. REN Y L, YANG L, GAO L, et al. Research progress and application of melanin metal chelate[J]. Materials Reports, 2020, 34(11): 145-152.
[21]	任顺成, 王国良, 王鹏, 等. 十种天然黑色素稳定性研究[J]. 中国粮油学报,2009,24(6):137−142. [REN S C, WANG G L, WANG P, et al. Stability of ten natural melanins[J]. Journal of the Chinese Cereals and Oils Association,2009,24(6):137−142. REN S C, WANG G L, WANG P, et al. Stability of ten natural melanins[J]. Journal of the Chinese Cereals and Oils Association, 2009, 24(6): 137-142.
[22]	杨喆, 万山, 张乔会, 等. 响应面法优化山杏核壳总黄酮提取工艺及其抗氧化性的研究[J]. 食品工业科技,2015,36(6):279−284. [YANG Z, WAN S, ZHANG Q H, et al. Optimization of extraction process and antioxidant activity of total flavonoids from apricot kernel and shell by response surface methodology[J]. Science and Technology of Food Industry,2015,36(6):279−284. YANG Z, WAN S, ZHANG Q H, et al. Optimization of extraction process and antioxidant activity of total flavonoids from apricot kernel and shell by response surface methodology[J]. Science and Technology of Food Industry, 2015, 36(6): 279-284.
[23]	李红姣, 李巨秀, 赵忠. 酶法辅助提取山杏种皮黑色素工艺优化及其稳定性[J]. 食品科学,2016,37(10):69−75. [LI H J, LI J X, ZHAO Z. Optimization and stability of enzymatic assisted extraction of melanin from seed coat of apricot[J]. Food Science,2016,37(10):69−75. doi: 10.7506/spkx1002-6630-201610012 LI H J, LI J X, ZHAO Z. Optimization and stability of enzymatic assisted extraction of melanin from seed coat of apricot[J]. Food Science, 2016, 37(10): 69-75. doi: 10.7506/spkx1002-6630-201610012
[24]	杨柳. 山杏核壳黑色素纳米颗粒的制备及性能研究[D]. 太原: 中北大学, 2019. YANG L. Preparation and properties of melanin nanoparticles in apricot kernel shell[D]. Taiyuan: North University of China, 2019.
[25]	李红姣, 李巨秀, 赵忠. 响应面试验优化超声波辅助提取山杏种皮黑色素工艺及其抗氧化活性[J]. 食品科学,2016(18):26−33. [LI H J, LI J X, ZHAO Z. Response surface optimization of ultrasonic-assisted extraction of melanin from testae of wild apricots and its antioxidant activities[J]. Food Science,2016(18):26−33. doi: 10.7506/spkx1002-6630-201618005 LI H J, LI J X, ZHAO Z. Response surface optimization of ultrasonic-assisted extraction of melanin from testae of wild apricots and its antioxidant activities[J]. Food Science, 2016, (18): 26-33. doi: 10.7506/spkx1002-6630-201618005
[26]	汪燕. 粒毛盘菌YM-346黑色素的结构、氨基酸修饰及生物活性研究[D]. 合肥: 合肥工业大学, 2012. WANG Y. Study on structure, amino acid modification and biological activity of YM-346 melanin from Trichoderma granuloides[D]. Hefei: Hefei University of Technology, 2012.
[27]	李军, 张乔会, 蓝晶晶, 等. 响应面法对山杏核壳黑色素提取工艺的研究[J]. 食品工业科技,2012(2):295−299. [LI J, ZHANG Q H, LAN J J, et al. Optimization of extraction process and antioxidant activity of total flavonoids from apricot kernel and shell by response surface methodology[J]. Science and Technology of Food Industry,2012(2):295−299. LI J, ZHANG Q H, LAN J J, et al. Optimization of extraction process and antioxidant activity of total flavonoids from apricot kernel and shell by response surface methodology[J]. Science and Technology of Food Industry, 2012, (2): 295-299.
[28]	徐利萍. 黑芝麻中黑色素的萃取、纯化及其性质的研究[D]. 无锡: 江南大学, 2006. XU L P. Study on extraction, purification and properties of melanin from black sesame[D]. Wuxi: Jiangnan University, 2006.
[29]	LONGO D L, STEFANIA R, CALLARI C, et al. Water soluble melanin derivatives for dynamic contrast enhanced photoacoustic imaging of tumor vasculature and response to antiangiogenic therapy[J]. Adv Healthc Mater,2017,6(1):1−6.
[30]	YE M, WANG Y, GUO G Y, et al. Physicochemical characteristics and antioxidant activity of arginine-modified melanin from Lachnum YM-346[J]. Food Chemistry,2012,135(4):2490−2497. doi: 10.1016/j.foodchem.2012.06.120
[31]	XIN C, MA J H, TAN C J, et al. Preparation of melanin from Catharsius molossus L. and preliminary study on its chemical structure[J]. Journal of Bioscience and Bioengineerin,2015,119(4):446−454. doi: 10.1016/j.jbiosc.2014.09.009
[32]	BUSZMAN E, PILAWA B, ZDYBEL M, et al. EPR examination of Zn²⁺, and Cu²⁺, effect on free radicals in DOPA-melanin-netilmicin complexes[J]. Chemical Physics Letters,2005,403(1−3):22−28. doi: 10.1016/j.cplett.2004.12.069
[33]	陈士国, 薛长湖, 薛勇, 等. 鱿鱼墨黑色素的自由基清除活性研究[J]. 中国海洋药物,2007,26(1):24−27. [CHEN S G, XUE C H, XUE Y, et al. Study on free radical scavenging activity of squid black pigment[J]. Chinese Journal of Marine Drugs,2007,26(1):24−27. doi: 10.3969/j.issn.1002-3461.2007.01.007 CHEN S G, XUE C H, XUE Y, et al. Study on free radical scavenging activity of squid black pigment[J]. Chinese Journal of Marine Drugs, 2007, 26(1): 24-27. doi: 10.3969/j.issn.1002-3461.2007.01.007
[34]	SZPOGANICZ B, GIDANIAN S, KONG P, et al. Metal chelating by melanins: Studies of colloidal dihydroxyindole-melanin, and its complexation by Cu(II) and Zn(II) ions[J]. Journal of Inorganic Biochemistry,2002,89(2):45−53.
[35]	汤陈鹏, 吕峰, 王蓉琳. 孔石莼多糖锌结构表征与体外降血糖活性[J]. 食品科学,2020,2020,41(7):52−58. [TANG C P, LÜ F, WANG R L. Structural characterization and hypoglycemic activity in vitro of ulva pertusa polysaccharides-zinc complex[J]. Food Science,2020,2020,41(7):52−58. doi: 10.7506/spkx1002-6630-20190320-263 TANG C P, LV F, WANG R L. Structural characterization and hypoglycemic activity in vitro of ulva pertusa polysaccharides-zinc complex[J]. Food Science, 2020, 2020, 41(7): 52-58. doi: 10.7506/spkx1002-6630-20190320-263
[36]	HONG L, SIMON J D. Current understanding of the chelating sites, capacity, affinity, and biological significance of metals in melanin[J]. Journal of Physical Chemistry B,2007,111(28):7938−7947. doi: 10.1021/jp071439h
[37]	姚增玉. 山杏种皮黑色素与Cu²⁺和Zn²⁺络合性质的研究[J]. 西北农林科技大学学报,2008,36(3):197−202. [YAO Z Y. Complexation of Cu²⁺ and Zn²⁺ with melanin in seed coat of apricot[J]. Journal of Northwest A & F University,2008,36(3):197−202. YAO Z Y. Complexation of Cu²⁺ and Zn²⁺ with melanin in seed coat of apricot[J]. Journal of Northwest A&F University, 2008, 36(3): 197-202.
[38]	雷敏. 鱿鱼墨黑色素及黑色素铁生物活性的研究[D]. 青岛: 中国海洋大学, 2008. LEI M. Study on free radical scavenging activity of squid black pigment[D]. Qingdao: Ocean University of China, 2008.
[39]	ATHIRA S, BIMLESH Mann, RAJAN Sharma, et al. Preparation and characterization of iron-chelating peptides from whey protein: An alternative approach for chemical iron fortification[J]. Food Research International,2021,141(3):110133.
[40]	ZHANG Y, DING X, LI M, et al. Preparation, characterization and in vitro stability of iron-chelating peptides from mung beans[J]. Food Chemistry,2021,349(1):129101.

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