FANG Huanle, ZHANG Jie, ZHAO Yuwen, et al. Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen[J]. Science and Technology of Food Industry, 2022, 43(14): 378−384. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100200.
Citation: FANG Huanle, ZHANG Jie, ZHAO Yuwen, et al. Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen[J]. Science and Technology of Food Industry, 2022, 43(14): 378−384. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100200.

Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen

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  • Received Date: October 20, 2021
  • Available Online: May 05, 2022
  • Objective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) group and VC group. The blank control group was intraperitoneally injected with normal saline every day, and the other groups were intraperitoneally injected with D-galactose (120 mg/kg) every day to induce the aging model. At the same time, the drug group was gavaged with high, medium and low doses of broken wall rape pollen suspension and VC solution, weighed every week, and the liver, spleen, kidney and thymus of mice were taken after 6 weeks to weigh the organ mass and calculate the organ index. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-PX) in serum and liver were determined by biochemical method. HE staining was used to observe the morphological changes of liver in aging mice. The expressions of Bax, Sirt1 and p53 proteins in the liver of aging mice were detected by Western Blot. Results: Compared with the aging model group, the medium and high dose groups of broken wall rape pollen could significantly improve the body mass, organ index of thymus, liver, spleen and kidney (P<0.05, P<0.01), significantly increase the contents of SOD, CAT and GSH-Px in serum and liver, reduce the content of MDA (P<0.05, P<0.01), improve hepatocyte swelling, make the expression of the apoptotic protein Bax and pro-aging protein p53 decrease, and the expression of anti-aging protein Sirt1 increase (P<0.05, P<0.01). Conclusion: Broken wall rape pollen can improve the aging of the body by anti-oxidation, anti apoptosis, regulating the content of aging protein.
  • [1]
    KCP M, CAV F, TB F, et al. Antiallergic effect of bee poleen phenolic extract and myricetin in ovalbuminsensitized mice[J]. Journal of Ethnopharmacology,2008,119(1):41−46. doi: 10.1016/j.jep.2008.05.036
    [2]
    郝转. 油菜花粉的功效及研究进展[J]. 粮食与油脂,2020,33(8):4−6. [HAO Z. Efficacy and research progress of rape pollen[J]. Grain and Oil,2020,33(8):4−6. doi: 10.3969/j.issn.1008-9578.2020.08.002

    HAO Z. Efficacy and research progress of rape pollen[J]. Grain and Oil, 2020, 33(8): 4-6. doi: 10.3969/j.issn.1008-9578.2020.08.002
    [3]
    CHEN H, WANG Z B, CHANG Q Y, et al. Determination of trace elements in rape honey and its corresponding rape flower and stem by ICP-MS[J]. Guang Pu Xue Yu Guang Pu Fen Xi,2014,34(2):548−552.
    [4]
    郭雪微, 卢梃, 鲍艳江, 等. 油菜蜂花粉黄酮类化合物抗氧化等作用的临床观察[J]. 北京中医药,2011,30(7):488−492. [GUO X L, LU T, BAO Y J, et al. Clinical observation on antioxidant effects of flavonoids from rape bee pollen[J]. Beijing Traditional Chinese Medicine,2011,30(7):488−492.

    GUO X L, LU T, BAO Y J, et al. Clinical observation on antioxidant effects of flavonoids from rape bee pollen[J]. Beijing Traditional Chinese Medicine, 2011, 30(7): 488-492.
    [5]
    郭雪微, 卢挺, 鲍艳江, 等. 油菜蜂花粉破壁前后黄酮类化合物及其它营养成分的比较[J]. 北京中医药,2010,29(9):700−702. [GUO X W, LU T, BAO Y J, et al. Comparison of flavonoids and other nutrients before and after wall breaking of rape bee pollen[J]. Beijing Traditional Chinese Medicine,2010,29(9):700−702.

    GUO X W, LU T, BAO Y J, et al. Comparison of flavonoids and other nutrients before and after wall breaking of rape bee pollen[J]. Beijing Traditional Chinese Medicine, 2010, 29(9): 700-702.
    [6]
    WU W, QIAO J T, XIAO X Y, et al. In vitro and in vivo digestion comparison of bee pollen with or without wall-disruption[J]. Journal of the Science of Food and Agriculture,2021,101(7):2744−2755. doi: 10.1002/jsfa.10902
    [7]
    游庭活, 温露, 刘凡. 衰老机制及延缓衰老活性物质研究进展[J]. 天然产物研究与开发,2015,27(11):1985−1990. [YOU T H, WEN L, LIU F. Research progress on aging mechanism and anti-aging active substances[J]. Research and Development of Natural Products,2015,27(11):1985−1990.

    YOU T H, WEN L, LIU F. Research progress on aging mechanism and anti-aging active substances[J]. Research and Development of Natural Products, 2015, 27(11): 1985-1990.
    [8]
    李凌娟, 胡庆亮. SIRT1与衰老分子机理研究进展[J]. 绿色科技,2013(5):292−295. [LI L J, HU Q L. Research progress of SIRT1 and molecular mechanism of aging[J]. Green Science and Technology,2013(5):292−295. doi: 10.3969/j.issn.1674-9944.2013.05.119

    LI L J, HU Q L. Research progress of SIRT1 and molecular mechanism of aging[J]. Green Science and Technology, 2013(5): 292-295. doi: 10.3969/j.issn.1674-9944.2013.05.119
    [9]
    王晶, 王勇, 李海龙, 等. 党参水提物对D-半乳糖致衰老小鼠肝脾形态结构和Bax蛋白及VEGF表达的影响[J]. 西北师范大学学报(自然科学版),2016,52(4):72−77. [WANG J, WANG Y, LI H L, et al. Effects of aqueous extract of Codonopsis pilosula on the morphological structure of liver and spleen and the expression of Bax protein and VEGF in aging mice induced by D-galactose[J]. Journal of Northwest Normal University (Natural Science Edition),2016,52(4):72−77.

    WANG J, WANG Y, LI H L, et al. Effects of aqueous extract of Codonopsis pilosula on the morphological structure of liver and spleen and the expression of Bax protein and VEGF in aging mice induced by D-galactose[J]. Journal of Northwest Normal University (Natural Science Edition), 2016, 52(4): 72-77.
    [10]
    王伟, 张晓丽, 李姗姗. 破壁油菜花粉在体抗氧化作用及量效[J]. 辽宁科技大学学报,2020,43(6):478−480. [WANG W, ZHANG X L, LI S S. Antioxidant effect and dose effect of wall broken rape pollen in vivo[J]. Journal of Liaoning University of Science and Technology,2020,43(6):478−480.

    WANG W, ZHANG X L, LI S S. Antioxidant effect and dose effect of wall broken rape pollen in vivo[J]. Journal of Liaoning University of Science and Technology, 2020, 43(6): 478-480.
    [11]
    李姗姗, 赵鸿仁, 王伟. 破壁油菜花粉配伍的体内抗氧化活性研究[J]. 辽宁科技大学学报,2020,43(3):237−240. [LI S S, ZHAO H R, WANG W. Study on the in vivo antioxidant activity of the pollen compatibility of broken rapeseed[J]. Journal of Liaoning University of Science and Technology,2020,43(3):237−240.

    LI S S, ZHAO H R, WANG W. Study on the in vivo antioxidant activity of the pollen compatibility of broken rapeseed[J]. Journal of Liaoning University of Science and Technology, 2020, 43(3): 237-240.
    [12]
    赵凡凡, 周玉枝, 高丽, 等. D-半乳糖致衰老大鼠模型的研究进展[J]. 药学学报,2017(3):347−354. [ZHAO F F, ZHOU Y Z, GAO L, et al. Research progress of aging rat model induced by D-galactose[J]. Journal of Pharmacy,2017(3):347−354.

    ZHAO F F, ZHOU Y Z, GAO L, et al. Research progress of aging rat model induced by D-galactose[J]. Journal of Pharmacy, 2017(3): 347-354.
    [13]
    徐叔云. 药理实验方法学[M].第三版. 北京: 人民卫生出版社, 2002.

    XU S Y. Methodology of pharmacological experiments[M]. Third Edition. Beijing: People’s Medica Publishing House, 2002.
    [14]
    卢春雪, 杨绍杰, 陶荟竹, 等. 衰老机制研究进展[J]. 中国老年学杂志,2018,38(1):248−250. [LU C X, YANG S J, TAO H Z, et al. Research progress on aging mechanism[J]. Chinese Journal of Gerontology,2018,38(1):248−250. doi: 10.3969/j.issn.1005-9202.2018.01.101

    LU C X, YANG S J, TAO H Z, et al. Research progress on aging mechanism[J]. Chinese Journal of Gerontology, 2018, 38(1): 248-250. doi: 10.3969/j.issn.1005-9202.2018.01.101
    [15]
    许梦然, 王迦琦, 高婧雯, 等. 北柴胡多糖对D-半乳糖致衰老模型小鼠的保护作用及其机制[J]. 吉林大学学报(医学版),2020,46(6):1215−1220. [XU M R, WANG J Q, GAO J W, et al. Protective effect of Bupleurum polysaccharide on aging model mice induced by D-galactose and its mechanism[J]. Journal of Jilin University (Medical Edition),2020,46(6):1215−1220.

    XU M R, WANG J Q, GAO J W, et al. Protective effect of Bupleurum polysaccharide on aging model mice induced by D-galactose and its mechanism[J]. Journal of Jilin University (Medical Edition), 2020, 46(6): 1215-1220.
    [16]
    赵凡凡, 李肖, 高丽, 等. 甘草水提物干预D-半乳糖致衰老大鼠的肝脏代谢组学研究[J]. 中草药,2017,48(17):3545−3553. [ZHAO F F, LI X, GAO L, et al. Intervention of licorice aqueous extract on liver metabolomics in aging rats induced by D-galactose[J]. Chinese Herbal Medicine,2017,48(17):3545−3553. doi: 10.7501/j.issn.0253-2670.2017.17.015

    ZHAO F F, LI X, GAO L, et al. Intervention of licorice aqueous extract on liver metabolomics in aging rats induced by D-galactose[J]. Chinese Herbal Medicine, 2017, 48(17): 3545-3553. doi: 10.7501/j.issn.0253-2670.2017.17.015
    [17]
    田勇, 郅琦, 李福香, 等. 昆仑胎菊水溶性黄酮提取工艺优化及其对衰老小鼠肝组织SIRT1、p53表达的影响[J]. 食品科学,2019,40(14):296−303. [TIAN Y, ZHI Q, LI F X, et al. Optimization of extraction process of water-soluble flavonoids from Kunlun foetal chrysanthemum and its effect on theexpression of SIRT1 and p53 in liver tissue of aging mice[J]. Food Science,2019,40(14):296−303. doi: 10.7506/spkx1002-6630-20181101-001

    TIAN Y, ZHI Q, LI F X, et al. Optimization of extraction process of water-soluble flavonoids from Kunlun foetal chrysanthemum and its effect on theexpression of SIRT1 and p53 in liver tissue of aging mice[J]. Food Science, 2019, 40(14): 296-303. doi: 10.7506/spkx1002-6630-20181101-001
    [18]
    何源, 王露露, 张晶. 多糖抗衰老作用机制的研究进展[J]. 中成药,2022,44(2):528−531. [HE Y, WANG L L, ZHANG J. Research progress on anti-aging mechanism of polysaccharides[J]. Chinese Patent Medicine,2022,44(2):528−531. doi: 10.3969/j.issn.1001-1528.2022.02.035

    HE Y, WANG L L, ZHANG J. Research progress on anti-aging mechanism of polysaccharides[J]. Chinese Patent Medicine, 2022, 44(2): 528-531. doi: 10.3969/j.issn.1001-1528.2022.02.035
    [19]
    徐舜, 崔红晶, 赵炜, 等. 细胞衰老分子机制的研究进展[J]. 广东医科大学学报,2020,38(1):1−10. [XU S, CUI H J, ZHAO W, et al. Research progress on molecular mechanism of cell aging[J]. Journal of Guangdong Medical University,2020,38(1):1−10. doi: 10.3969/j.issn.1005-4057.2020.01.001

    XU S, CUI H J, ZHAO W, et al. Research progress on molecular mechanism of cell aging[J]. Journal of Guangdong Medical University, 2020, 38(1): 1-10. doi: 10.3969/j.issn.1005-4057.2020.01.001
    [20]
    刘玥辰, 阎星旭, 赵换, 等. 衰老的分子机制及相关代谢组学研究[J]. 天津中医药大学学报,2021,40(2):267−272. [LIU Y C, YAN X X, ZHAO H, et al. Molecular mechanism of aging and related metabolomics[J]. Journal of Tianjin University of Traditional Chinese Medicine,2021,40(2):267−272.

    LIU Y C, YAN X X, ZHAO H, et al. Molecular mechanism of aging and related metabolomics[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2021, 40(2): 267-272.
    [21]
    彭壮, 李弘, 彭莎, 等. 玉竹多糖对D-半乳糖诱导衰老小鼠认知障碍的改善[J]. 中华中医药学刊,2020,38(11):254−258. [PENG Z, LI H, PENG S, et al. Effect of Polygonatum odoratum polysaccharide on cognitive impairment in aging mice induced by D-galactose[J]. Chinese Journal of Traditional Chinese Medicine,2020,38(11):254−258.

    PENG Z, LI H, PENG S, et al. Effect of Polygonatum odoratum polysaccharide on cognitive impairment in aging mice induced by D-galactose[J]. Chinese Journal of Traditional Chinese Medicine, 2020, 38(11): 254-258.
    [22]
    冯慧, 焦士蓉, 刘佳, 等. 油菜花研究进展[J]. 食品工业,2011,12(9):108−111. [FENG H, JIAOS R, LIU J, et al. Research progress of rape flower[J]. Food Industry,2011,12(9):108−111.

    FENG H, JIAOS R, LIU J, et al. Research progress of rape flower[J]. Food Industry, 2011, 12(9): 108-111.
    [23]
    折丽婷, 赵彩虹, 陈春红, 等. 橄榄苦苷对D-半乳糖诱导衰老小鼠的抗衰老作用研究[J]. 中国中医药科技,2020,27(1):37−40. [ZHE L T, ZHAO C H, CHEN C H, et al. Study on anti-aging effect of oleopicroside on aging mice induced by D-galactose[J]. China Science and Technology of Traditional Chinese Medicine,2020,27(1):37−40.

    ZHE L T, ZHAO C H, CHEN C H, et al. Study on anti-aging effect of oleopicroside on aging mice induced by D-galactose[J]. China Science and Technology of Traditional Chinese Medicine, 2020, 1(27): 37-40.
    [24]
    柳江枫, 杨晔宏, 武乐, 等. 自然衰老C57BL/6J雄性小鼠肝脏的定量与乙酰化修饰蛋白质组学特征[J]. 中国医学科学院学报,2021,43(5):696−705. [LIU J F, YANG Y H, WU L, et al. Quantitative and acetylation modified proteomic characteristics of liver in naturally aging C57BL/6J male mice[J]. Journal of Chinese Academy of Medical Sciences,2021,43(5):696−705.

    LIU J F, YANG Y H, WU L, et al. Quantitative and acetylation modified proteomic characteristics of liver in naturally aging C57BL/6J male mice[J]. Journal of Chinese Academy of Medical Sciences, 2021, 43(5): 696-705.
    [25]
    薛亚兰, 许晶廷, 邱继红. 淫羊藿苷对抑郁症大鼠抑郁样行为和神经元损伤的影响[J]. 临床和实验医学杂志,2021,20(20):2152−2156. [XUE Y L, XU J T, QIU J H. Effects of Icariin on depression like behavior and neuronal damage in depressed rats[J]. Journal of Clinical and Experimental Medicine,2021,20(20):2152−2156. doi: 10.3969/j.issn.1671-4695.2021.20.007

    XUE Y L, XU J T, QIU J H. Effects of Icariin on depression like behavior and neuronal damage in depressed rats[J]. Journal of Clinical and Experimental Medicine, 2021, 20(20): 2152-2156. doi: 10.3969/j.issn.1671-4695.2021.20.007
    [26]
    LI Q S, ZENG J C, SU M L, et al. Acetylshikonin from Zicao attenuates cognitive impairment and hippocampus senescence in d-galactose-induced aging mouse model via upregulating the expression of SIRT1[J]. Brain Research Bulletin,2018,137:311−318. doi: 10.1016/j.brainresbull.2018.01.007
    [27]
    ZHU R Z, LI B S, GAO S S, et al. Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53[J]. Environmental Pollution,2021,25(4):297−305.
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