Antioxidant and Anti-aging Mechanism of Wall Broken Rape Pollen

Objective: To investigate the antioxidant and anti-aging effects of wall broken rape pollen on aging model mice induced by D-galactose. Methods: 48 mice were randomly divided into blank control group, aging model group, wall broken rape pollen high (560 mg/kg), medium (280 mg/kg), low (140 mg/kg) group and V_C group. The blank control group was intraperitoneally injected with normal saline every day, and the other groups were intraperitoneally injected with D-galactose (120 mg/kg) every day to induce the aging model. At the same time, the drug group was gavaged with high, medium and low doses of broken wall rape pollen suspension and V_C solution, weighed every week, and the liver, spleen, kidney and thymus of mice were taken after 6 weeks to weigh the organ mass and calculate the organ index. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-PX) in serum and liver were determined by biochemical method. HE staining was used to observe the morphological changes of liver in aging mice. The expressions of Bax, Sirt1 and p53 proteins in the liver of aging mice were detected by Western Blot. Results: Compared with the aging model group, the medium and high dose groups of broken wall rape pollen could significantly improve the body mass, organ index of thymus, liver, spleen and kidney (P<0.05, P<0.01), significantly increase the contents of SOD, CAT and GSH-Px in serum and liver, reduce the content of MDA (P<0.05, P<0.01), improve hepatocyte swelling, make the expression of the apoptotic protein Bax and pro-aging protein p53 decrease, and the expression of anti-aging protein Sirt1 increase (P<0.05, P<0.01). Conclusion: Broken wall rape pollen can improve the aging of the body by anti-oxidation, anti apoptosis, regulating the content of aging protein.