Study on Extraction Technology and Pharmacological Activities of Polysaccharide from <i>Ziziphus jujube</i>

GONG Pin; WANG Peipei; TONG Meilin; LONG Hui; MAO Gennian; CHEN Fuxin

doi:10.13386/j.issn1002-0306.2021100105

Volume 43 Issue 13

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(13): 198-207. > DOI: 10.13386/j.issn1002-0306.2021100105

GONG Pin, WANG Peipei, TONG Meilin, et al. Study on Extraction Technology and Pharmacological Activities of Polysaccharide from Ziziphus jujube[J]. Science and Technology of Food Industry, 2022, 43(13): 198−207. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100105.

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Study on Extraction Technology and Pharmacological Activities of Polysaccharide from Ziziphus jujube

1.
School of Food and Bioengineering, Shaanxi University of Science & Technology, Xi’an 710021, China
2.
College of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China

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Received Date: October 12, 2021
Available Online: April 29, 2022

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Abstract

Abstract

Polysaccharide from Ziziphus jujube Mill. (ZJP) was extracted by ultrasound-assisted extraction. Taking the extraction rate as the evaluation index, the extraction process of polysaccharide was optimized by Design-Expert8.0 and orthogonal experiment on the basis of single-factor test. Subsequently, DPPH free radical and hydroxyl free radical scavenging abilities were used as indicators to evaluate the antioxidant effect of polysaccharides, and the inhibitory rates of α-amylase and α-glucosidase were used as indicators to prove that jujube polysaccharides had the potential of reducing blood sugar. Finally, an external model was established to explore its hypoglycemic mechanism. The optimum parameters were as follows: ultrasonic time of 40 min, solid-liquid ratio of 1:20, ultrasonic power of 80 W, water-bath incubation time of 30 min. Under these conditions the extraction rate of ZJP was 6.58%, which was consistent with the theoretical prediction value of 6.71%. Moreover, the antioxidant activity of ZJP was determined by DPPH free radical and hydroxyl free radical scavenging system and iron-reducing power. In addition, when the polysaccharide concentration reached 14 mg/mL, the inhibition rate of α-glucosidase was 51.56%, and when the concentration was 4 mg/mL, the inhibition rate of α-amylase was 28.43%, respectively, confirming that jujube polysaccharide had obvious hypoglycemic activity. On this basis, the insulin resistance HepG2 cell model was used to detect the glucose consumption effect of different concentrations of jujube polysaccharides by glucose kit and MTT method. The results showed that when the concentration of the drug was 1.0 mg/mL, the cell's consumption of glucose was the largest, GT/MTT reached 154.2% of the model group. Western blotting was used to detect the protein expression level of PI3K/Akt pathway in insulin-resistant HepG2 cells, and it was determined that ZJP could alleviate insulin resistance and exert cytoprotective effect by activating PI3K/Akt pathway. In summary, the extraction method of jujube polysaccharide was optimized, and it was confirmed that jujube polysaccharide had anti-oxidant activity and hypoglycemic activity in vitro, and could be used as a potential anti-oxidant or functional food with a homology of medicine and food.
- Ziziphus jujube Mill.,
- response surface methodology,
- polysaccharide,
- antioxidant activity,
- hypoglycemic activity

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References (34)

References

[1]	GAO Q H, WU C S, WANG M. The jujube (Ziziphus jujuba Mill.) fruit: A review of current knowledge of fruit composition and health benefits[J]. J Agric Food Chem,2013,61(14):51−63.
[2]	JI X L, PENG Q, YUAN Y P, et al. Isolation, structures and bioactivities of the polysaccharides from jujube fruit (Ziziphus jujuba Mill.): A review[J]. Food Chemistry,2017,227:49−57.
[3]	I X, LIU F, PENG Q, et al. Purification, structural characterization, and hypolipidemic effects of a neutral polysaccharide from Ziziphus jujuba cv. Muzao[J]. Food Chem,2018,245:24−30.
[4]	LIN X, JI X, WANG M, et al. An alkali-extracted polysaccharide from Zizyphus jujuba cv. Muzao: Structural characterizations and antioxidant activities[J]. Int J Biol Macromol,2019,136:7−15.
[5]	WANG B, HUI Y, LIU L, et al. Optimized extraction of phenolics from jujube peel and their anti-inflammatory effects in LPS-stimulated murine macrophages[J]. J Agric Food Chem,2019,67(6):66−73.
[6]	KHAN M N, UL HAQ F, RAHMAN S, et al. Metabolite distribution and correlation studies of Ziziphus jujuba and Ziziphus nummularia using LC-ESI-MS/MS[J]. Journal of Pharmaceutical and Biomedical Analysis,2020,178:18.
[7]	KAWABATA K, KITAMURA K, IRIE K, et al. Triterpenoids isolated from Ziziphus jujuba enhance glucose uptake activity in skeletal muscle cells[J]. Journal of Nutritional Science and Vitaminology,2017,63(3):193−199. doi: 10.3177/jnsv.63.193
[8]	LI Y, GUO S, HUA T, et al. Comparative pharmacokinetics of triterpenic acids in normal and immunosuppressed rats after oral administration of Jujuba fructus extract by UPLC-MS/MS[J]. J Chromatogr B Analyt Technol Biomed Life Sci,2018,1077-1078:13−21. doi: 10.1016/j.jchromb.2018.01.026
[9]	GUO S, DUAN J A, ZHANG Y, et al. Contents changes of triterpenic acids, nucleosides, nucleobases, and saccharides in jujube (Ziziphus jujuba) fruit during the drying and steaming process[J]. Molecules,2015,20(12):29−40.
[10]	朱星宇, 郭东起. 红枣关键功能成分及其生物活性的研究进展[J]. 食品研究与开发,2021,42(8):197−201. [ZHU Xingyu, GUO Dongqi. Research advancement on key functional components and biological activities of red jujubes[J]. Food Research and Development,2021,42(8):197−201. ZHU Xingyu, GUO Dongqi. Research advancement on key functional components and biological activities of red jujubes[J]. Food Research and Development, 2021, 42(8): 197-201.
[11]	ZHAO Z, LIU M, TU P. Characterization of water soluble polysaccharides from organs of Chinese Jujube (Ziziphus jujuba Mill. cv. Dongzao)[J]. European Food Research and Technology,2008,26(5):985−989.
[12]	LIANG Q, WANG X, YANG S, et al. Characterization of the antioxidative polysaccharides from Ziziphus jujube cv. Goutouzao and its tumor-inhibitory effects on human colorectal carcinoma LoVo cells via immunocyte activation[J]. J Food Biochem,2020,44(11):62.
[13]	YUE Y, WU S, LI Z, et al. Wild jujube polysaccharides protect against experimental inflammatory bowel disease by enabling enhanced intestinal barrier function[J]. Food Funct,2015,6(8):68−77.
[14]	LIU Y, ZHAO X, LIN T, et al. Molecular mechanisms of polysaccharides from Ziziphus jujuba Mill var. spinosa seeds regulating the bioavailability of spinosin and preventing colitis[J]. Int J Biol Macromol,2020,163:1393−1402. doi: 10.1016/j.ijbiomac.2020.07.229
[15]	ZHANG L, LIU X, WANG Y, et al. In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv. Muzao[J]. Int J Biol Macromol,2017,95:19−25.
[16]	HONG S, KIM Y, SUNG J, et al. Jujube (Ziziphus jujuba Mill.) protects hepatocytes against alcohol-induced damage through Nrf2 activation[J]. Evid Based Complement Alternat Med,2020,2020:31.
[17]	WANG J A, HUANG L F, REN Q, et al. Polysaccharides of Scrophularia ningpoensis Hemsl.: Extraction, antioxidant, and anti-inflammatory evaluation[J]. Evid-Based Compl Alt,2020,2020:1−13.
[18]	HU J, LIU Y, CHENG L, et al. Comparison in bioactivity and characteristics of Ginkgo biloba seed polysaccharides from four extract pathways[J]. Int J Biol Macromol,2020,159:56−64.
[19]	CHEN Z, YIN C, FAN X, et al. Characterization of physicochemical and biological properties of Schizophyllum commune polysaccharide extracted with different methods[J]. Int J Biol Macromol,2020,156:25−34.
[20]	JI X, PENG Q, YUAN Y, et al. Extraction and physicochemical properties of polysaccharides from Ziziphus Jujuba cv. Muzao by ultrasound-assisted aqueous two-phase extraction[J]. Int J Biol Macromol,2018,8:41−49.
[21]	黎云龙, 于震宇, 郜海燕, 等. 骏枣多糖提取工艺优化及其抗氧化活性[J]. 食品科学,2015,36(4):45−49. [LI Yunlong, YU Zhenyu, DAO Haiyan, et al. Optimization of extraction process and antioxidant capacity of polysaccharides from Zizyphus jujuba cv. Junzao[J]. Food Science,2015,36(4):45−49. doi: 10.7506/spkx1002-6630-201504009 LI Yunlong, YU Zhenyu, DAO Haiyan, et al. Optimization of extraction process and antioxidant capacity of polysaccharides from Zizyphus jujuba cv. Junzao[J]. Food Science, 2015, 36(4): 45-49. doi: 10.7506/spkx1002-6630-201504009
[22]	李栋, 薛瑞婷. 响应面耦合遗传算法优化超声辅助复合酶提取红枣多糖工艺[J]. 食品研究与开发,2021,42(6):96−103. [LI Dong, XUE Ruiting. Optimization of ultrasonic-assisted complex enzyme extraction polysaccharides from jujube by response surface methodology coupled with genetic algorithm[J]. Food Research and Development,2021,42(6):96−103. LI Dong, XUE Ruiting. Optimization of ultrasonic-assisted complex enzyme extraction polysaccharides from jujube by response surface methodology coupled with genetic algorithm[J]. Food Research and Development 2021, 42(6): 96-103.
[23]	LI J, AI L, HANG F, et al. Composition and antioxidant activity of polysaccharides from jujuba by classical and ultrasound extraction[J]. Int J Biol Macromol,2014,63:150−153. doi: 10.1016/j.ijbiomac.2013.10.043
[24]	杨海涛, 曹小燕. 超声辅助浸提拐枣中多糖及抗氧化性研究[J]. 应用化工,2017,46(11):74−77. [YANG Haitao, CAO Xiaoyan. Study on ultrasonic wave assisted extraction of polysaccharide from Hovenia dulcis and its antioxidant activity[J]. Applied Chemical Industry,2017,46(11):74−77. YANG Haitao, Cao Xiaoyan. Study on ultrasonic wave assisted extraction of polysaccharide from Hovenia dulcis and its antioxidant activity[J]. Applied Chemical Industry, 2017, 46(11): 74-77.
[25]	QU C, YU S, LUO L, et al. Optimization of ultrasonic extraction of polysaccharides from Ziziphus jujuba Mill. by response surface methodology[J]. Chem Cent J,2013,7(1):160. doi: 10.1186/1752-153X-7-160
[26]	刘晓茵, 赵帅, 路芳, 等. 半枝莲多糖的Sevage法除蛋白工艺研究[J]. 中国食物与营养,2016,22(3):56−58. [LIU Xiaoyin, ZHAO Shuai, LU Fang, et al. Deproteinization process of Barbata polysaccharide with Sevage method[J]. Food and Nutrition in China,2016,22(3):56−58. LIU Xiaoyin, ZHAO Shuai, LU Fang, et al. Deproteinization process of Barbata polysaccharide with Sevage method[J]. Food and Nutrition in China 2016, 22(3): 56-58.
[27]	陈凤, 赵万岭, 王星仪, 等. 苯酚-硫酸法测定明日叶根中多糖含量[J]. 化工管理,2019,4(26):11−13. [CHEN Feng, ZHAO Wanling, WNG Xingyi, et al. Phenol-sulfuric acid method for determination of polysaccharides in Ashitaba root[J]. Chemical Enterprise Management,2019,4(26):11−13. CHEN Feng, Zhao Wanling, WNG Xingyi, et al. Phenol-sulfuric acid method for determination of polysaccharides in Ashitaba root[J]. Chemical Enterprise Management 2019, 4(26): 11-13.
[28]	王文平, 郭祀远, 李琳, 等. 考马斯亮蓝法测定野木瓜多糖中蛋白质的含量[J]. 食品研究与开发,2008(1):115−117. [WANG Zhiwen, GUO Siyuan, LI Lin, et al. Determination of protein content in polysaccharides from Stanuntonia chinensis with Coomassie brilliant blue method[J]. Food Research and Development,2008(1):115−117. WANG Zhiwen, GUO Siyuan, LI Lin, et al. Determination of protein content in polysaccharides from Stanuntonia chinensis with Coomassie brilliant blue method[J]. Food Research and Development 2008(1): 115-117.
[29]	王金凤, 王芳, 王国玉, 等. 胰岛素抵抗HepG2细胞模型建立的正交实验研究[J]. 中南药学,2021,19(1):67−72. [WANG Jinfeng, WANG Fang, WANG Guoyu, et al. Establishment of insulin resistant HepG2 cell model by orthogonal test[J]. Central South Pharmacy,2021,19(1):67−72. WANG Jinfeng, WANG Fang, WANG Guoyu, et al. Establishment of insulin resistant HepG2 cell model by orthogonal test[J]. Central South Pharmacy, 2021, 19(1): 67-72.
[30]	方飞, 吴新荣, 罗明俐, 等. HepG2细胞胰岛素抵抗模型的建立及在筛选桑叶有效部位中的应用[J]. 医药导报,2012,31(6):691−694. [FANG Fei, WU Xinrong, LUO Mingli, et al. Establishment of insulin resistant HepG2 cell model and its application in screening bioactive components of mulberry leaves[J]. Herald of Medicine,2012,31(6):691−694. FANG Fei, WU Xinrong, LUO Mingli, et al. Establishment of insulin resistant HepG2 cell model and its application in screening bioactive components of mulberry leaves[J]. Herald of Medicine 2012, 31(6): 691-694.
[31]	CHEN L, ZHANG Y, JIN L, et al. Preparation, characterization and antioxidant activity of polysaccharide from Fallopia multiflora (Thunb.) Harald[J]. Int J Biol Macromol,2018,108:259−262. doi: 10.1016/j.ijbiomac.2017.12.020
[32]	WU Q, LUO M, YAO X, et al. Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis tangshen Oliv[J]. Carbohydr Polym,2020,236:20.
[33]	REAVEN G M. Insulin resistance: The link between obesity and cardiovascular disease[J]. Endocrinology and Metabolism Clinics of North America,2008,37(3):581−601. doi: 10.1016/j.ecl.2008.06.005
[34]	STEINBERG G R, KEMP B E. AMPK in health and disease[J]. Physiological Reviews,2009,89(3):1025−1078. doi: 10.1152/physrev.00011.2008