Protective Effect and Mechanism of D-ribose on Doxorubicin-induced Cardiotoxicity

XIAO Aiai; WANG Xueyan; WEN Min; WANG Zhengping

doi:10.13386/j.issn1002-0306.2021100074

Volume 43 Issue 13

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(13): 359-366. > DOI: 10.13386/j.issn1002-0306.2021100074

XIAO Aiai, WANG Xueyan, WEN Min, et al. Protective Effect and Mechanism of D-ribose on Doxorubicin-induced Cardiotoxicity[J]. Science and Technology of Food Industry, 2022, 43(13): 359−366. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100074.

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Protective Effect and Mechanism of D-ribose on Doxorubicin-induced Cardiotoxicity

XIAO Aiai^1,,
WANG Xueyan¹,
WEN Min^1, ,,
WANG Zhengping^{1, 2, 3, ,}

1.
Institute of Biopharmaceutical, Liaocheng University, Liaocheng 252000, China
2.
Liaocheng High-tech Biotechnology Co., Ltd., Liaocheng 252000, China
3.
Haimen Pharmaceutical Technology Co., Ltd., Nantong 226133, China

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Received Date: October 12, 2021
Available Online: April 21, 2022

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Abstract

Abstract

Objective: To study the protective effect and mechanism of D-ribose on Doxorubicin (DOX)-induced cardiotoxicity in mice. Methods: Eight-week-old male ICR mice were randomly divided into normal group (Con), model (DOX) group, D-ribose low-dose group (LDR) and D-ribose high-dose group (HDR), with 10 mice in each group. DOX acute cardiotoxicity mouse model was established by a single intraperitoneal injection of high dose doxorubicin (15 mg/kg). Lactate dehydrogenase (LDH) activity levels in serum and adenosine triphosphate (ATP) content in heart tissue were detected by the commercial kits. The pathological changes of myocardial tissue were observed by hematoxylin-eosin stain (HE) staining. Cardiac oxidative stress was assessed by measuring the activities of total superoxide dismutase (T-SOD), catalase (CAT) and malondialdehyde (MDA) in myocardial tissue. The levels of silent mating type information regulation 2 homolog 1 (Sirt1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteine-containing aspartate specific protease 3 (Caspase-3) were detected by Western blotting. Results: DOX could significantly reduce the body weight of mice (P<0.05), serum LDH activity was significantly increased (P<0.05), antioxidant enzyme activity significantly decreased (P<0.05); Oral D-ribose significantly decreased LDH levels (P<0.05) and increased antioxidant enzyme activity (P<0.05), increased Sirt1, PGC-1α, Bcl-2 protein expression levels (P<0.05), decreased Caspase-3, Bax protein expression levels (P<0.05). Conclusion: D-ribose could alleviate the DOX-induced acute cardiotoxicity by activating Sirt1/PGC-1α pathway, inhibiting oxidative stress and apoptosis.
- D-ribose,
- doxorubicin,
- cardiotoxicity,
- apoptosis,
- oxidative stress

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References

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