LIANG Yunxia, XIE Suya, LI Hongbo, et al. Analysis of Lipid Composition and Physicochemical Properties of Yak Bone[J]. Science and Technology of Food Industry, 2022, 43(13): 251−257. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100010.
Citation: LIANG Yunxia, XIE Suya, LI Hongbo, et al. Analysis of Lipid Composition and Physicochemical Properties of Yak Bone[J]. Science and Technology of Food Industry, 2022, 43(13): 251−257. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100010.

Analysis of Lipid Composition and Physicochemical Properties of Yak Bone

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  • Received Date: October 10, 2021
  • Available Online: April 30, 2022
  • In order to improve yak bone utilization rate and realize high-value utilization, the lipid composition and physicochemical parameters of yak bone were determined and analyzed by GC-MS and IUPAC method. A total of 20 fatty acids were detected by lipidomic analysis, of which SFA (Saturated fatty acid) content accounted for 41.75%, FA n:1 (Monounsaturated fatty acid) 53.01%, PUFA (Polyunsaturated fatty acid) 1.46%, and others 3.79%. A total of four lipids were detected in negative ion mode: Car (Acetyl L-Carnitine), SM (Sphingomyelines), PC (Phosphatidylcholines) and TAG (Triacylglycerols), and the contents were PC>SM>TAG>Car in order. The results of physical and chemical indexes showed that the fat of yak leg bone had better stability and more saturated fatty acids. Two substances, α-trienol and γ-trienol, were detected in yak leg bones. The oxidation stability of oil showed that the induction time of yak bone oil was 5.56 h, the oxidation stability of yak bone oil was high, and it had strong anti-oxidation effect. Fatty acids are closely related to bone health. Yak bone is rich in fatty acids. Therefore, yak bone can be used as a potential nutrient to maintain bone health.
  • [1]
    陈攀攀, 柴志欣, 钟金城. 牦牛线粒体基因的研究进展及应用[J]. 黑龙江畜牧兽医,2015(19):54−56. [CHEN P P, CHAI Z X, ZHONG J C. Yak the research progress and application of mitochondrial genes[J]. Heilongjiang Animal Husbandry and Veterinary,2015(19):54−56.

    CHEN P P, CHAI Z X, ZHONG J C. Yak the research progress and application of mitochondrial genes[J]. Heilongjiang Animal Husbandry and Veterinary, 2015(19): 54-56.
    [2]
    倪蕴琪, 唐静仪, 刘怀高, 等. 牦牛骨资源综合利用的研究现状与展望[J]. 农产品加工,2020(22):87−92. [NI Y Q, TANG J Y, LIU H G, et al. Yak bone research status quo and outlook of resources comprehensive utilization[J]. Agricultural Products Processing,2020(22):87−92.

    NI Y Q, TANG J Y, LIU H G, et al. Yak bone research status quo and outlook of resources comprehensive utilization[J]. Agricultural Products Processing, 2020(22): 87-92.
    [3]
    曹兵海, 张越杰, 李俊雅, 等. 2020年度肉牛牦牛产业技术发展报告[J]. 中国畜牧杂志,2021,57(3):240−245. [CAO H B, ZHANG Y J, LI J Y, et al. The beef cattle yak industry technology development report 2020[J]. Chinese Journal of Animal Husbandry,2021,57(3):240−245.

    CAO H B, ZHANG Y J, LI J Y, et al. The beef cattle yak industry technology development report 2020[J]. Chinese Journal of Animal Husbandry, 2021, 57(3): 240-245.
    [4]
    刘宇, 史梦妮, 付骞卉, 等. 牦牛骨及其功效活性研究进展[J]. 中央民族大学学报(自然科学版),2021,30(1):67−73. [LIU Y, SHI M N, FU Q H, et al. The research of progress of the yak bone and its effect[J]. Journal of Central University for Nationalities(Natural Science Edition),2021,30(1):67−73.

    LIU Y, SHI M N, FU Q H, et al. The research of progress of the yak bone and its effect[J]. Journal of Central University for Nationalities(Natural Science Edition), 2021, 30(1): 67-73.
    [5]
    周利兵. 青海牦牛骨主要矿物质元素的主成分分析[J]. 云南民族大学学报(自然科学版),2006,15(2):141−143. [ZHOU L B. Qinghai yak bone mineral element main principal component analysis[J]. Journal of Yunnan University for Nationalities(Natural Science Edition),2006,15(2):141−143.

    ZhOU L B. Qinghai yak bone mineral element main principal component analysis[J]. Journal of Yunnan University for Nationalities(Natural Science Edition), 2006, 15(2): 141-143.
    [6]
    张春晖, 叶孟亮, 郭玉杰, 等. 骨肽干预治疗骨质疏松中的生物标志物、筛选方法及用途[P]. 北京市: CN111007170B, 2021-06-29.

    ZHANG C H, YE M L, GUO Y J, et al. Biomarkers of bone peptide intervention in the treatment of osteoporosis, screening methods and purposes[P]. The Beijing Municipal: CN111007170B, 2021-06-29.
    [7]
    瞿瑗, 李诚, 程乐涛, 等. 牦牛骨免疫活性肽的酶解制备研究[J]. 食品工业科技,2016,37(3):271−274,278. [QU Y, LI C, CHENG L T, et al. Yak bone immune active peptide enzyme hydrolysis of research[J]. Science and Technology of Food Industry,2016,37(3):271−274,278.

    QU Y, LI C, CHENG L T, et al. Yak bone immune active peptide enzyme hydrolysis of research[J]. Science and Technology of Food Industry, 2016, 37(3): 271-274, 278.
    [8]
    吴婷, 贾伟, 管声, 等. 5个品种牛骨的营养组成及其含量差异分析[J]. 食品工业科技,2017,38(2):342−348. [WU T, JIA W, GUAN S, et al. Five varieties of bone nutrition composition and its content variance analysis[J]. Science and Technology of Food Industry,2017,38(2):342−348.

    WU T, JIA W, GUAN S, et al. Five varieties of bone nutrition composition and its content variance analysis[J]. Science and Technology of Food Industry, 2017, 38(2): 342-348.
    [9]
    姜海洋, 张周莉, 孙敏, 等. 牦牛骨抗氧化肽的分离纯化及鉴定[J]. 基因组学与应用生物学,2019,38(8):3479−3485. [JIANG H Y, ZHANG Z L, SUN M, et al. Yak bone antioxidant peptide purification and identification[J]. Genomics and Applied Biology,2019,38(8):3479−3485.

    JIANG H Y, ZHANG Z L, SUN M, et al. Yak bone antioxidant peptide purification and identification[J]. Genomics and Applied Biology, 2019, 38(8): 3479-3485.
    [10]
    刘静, 李诚, 胡迤萧, 等. 酶解法制备牦牛骨胶原多肽及其加工性能评价[J]. 食品与生物技术学报,2018,37(5):547−554. [LIU J, LI C, HU Y X, et al. Enzyme hydrolysis yak collagen peptide and its processing and performance evaluation[J]. Journal of Food and Aiological Technology,2018,37(5):547−554.

    LIU J, LI C, HU Y X, et al. Enzyme hydrolysis yak collagen peptide and its processing and performance evaluation[J]Journal of Food and Aiological Technology, 2018, 37(5): 547-554.
    [11]
    刘静. 牦牛骨胶原多肽螯合钙的制备研究[D]. 成都: 四川农业大学, 2016.

    LIU J. Yak collagen peptides chelating calcium preparation research[D]. Chengdu: Sichuan Agricultural University, 2016.
    [12]
    秦晓洁. 牦牛骨粉制备及其抗骨质疏松活性研究[D]. 北京: 中国农业科学院, 2019.

    QING X J. Yak bone powder preparation and research of the anti osteoporosis[D]. Beijing: The Chinese Academy of Agricultural Sciences, 2019.
    [13]
    YE M L, JIA W, ZHANG C H, et al. Valorization of yak (Bos grunniens) bones as sources of functional ingredients[J]. Waste and Biomass Valorization,2021,12(3):1553−1564. doi: 10.1007/s12649-020-01078-2
    [14]
    张志平, 白晓春, 金大地. n-3多不饱和脂肪酸与骨代谢[J]. 中国骨质疏松杂志,2011,17(6):556−560. [ZHANG Z P, BAI X C, JIN D D. n-3 Polyunsaturated fatty acids and bone metabolism[J]. Chinese Journal of Osteoporosis,2011,17(6):556−560. doi: 10.3969/j.issn.1006-7108.2011.06.022

    ZHANG Z P, BAI X C, JIN D D. n-3 polyunsaturated fatty acids and bone metabolism. [J]. Chinese Journal of Osteoporosis, 2011, 17(6): 556-560. doi: 10.3969/j.issn.1006-7108.2011.06.022
    [15]
    陈昭莹, 简盟月, 郑金宝. 骨质健康与营养[J]. 物理治疗学会杂志,2016,41(1):20−27. [CHEN S Y, JIAN Y Y, ZHENG J B. Bone health and nutrition[J]. Journal of the Society of Physiotherapy,2016,41(1):20−27.

    CHEN S Y, JIAN Y Y, ZHENG J B. Bone health and nutrition[J]. Journal of the Society of Physiotherapy, 2016, 41(1): 20-27.
    [16]
    BAO M, ZHANG K, WEI Y, et al. Therapeutic potentials and modulatory mechanisms of fatty acids in bone[J]. Cell Proliferation,2019,53(2):e12735.
    [17]
    BRUCE A WATKINS, LI Y, MARK F Seifert. Nutraceutical fatty acids as biochemical and molecular modulators of skeletal biology[J]. Journal of the American College of Nutrition,2001,20(5):410−416.
    [18]
    WATKINS B A. Regulatory effects of polyunsaturates on bone modeling and cartilage function[J]. World Review of Nutrition and Dietetics,1998(83):38−51.
    [19]
    MCMAHON MARK S. Beneficial effect of omega-3 fatty acids on bone metabolism[J]. Orthopedics,2012,35(9):735. doi: 10.3928/01477447-20120822-42
    [20]
    ELAGIZI A, LAVIE CARL J, O'KEEFE Evan, et al. An update on omega-3 polyunsaturated fatty acids and cardiovascular health[J]. Nutrients,2021,13(1):204. doi: 10.3390/nu13010204
    [21]
    FOLCH J. A simple method for the isolation and purification of total lipids from animal tissues[J]. J Biol Chem,1957,226(1):497−509. doi: 10.1016/S0021-9258(18)64849-5
    [22]
    WANG M, WANG C Y, HAN X L. Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why?[J]. Mass Spectrometry Reviews,2017,36(6):693−714. doi: 10.1002/mas.21492
    [23]
    HAN X, YANG K, GROSS R W. Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: Development of an automated high-throughput platform for shotgun lipidomics[J]. Rapid Communications in Mass Spectrometry,2010,22(13):2115−2124.
    [24]
    YANG K, CHENG H, GROSS R W, et al. Automated lipid identification and quantification by multidimensional mass spectrometry based shotgun lipidomics[J]. Analytical Chemistry,2009,81(11):4356−4368. doi: 10.1021/ac900241u
    [25]
    PAQUOT C, HAUTFENNE A. Standard methods for the analysis of oils, fats and derivatives, 7th edn. : Blackwell, Oxford, 1987(ISBN 0632-015861). xvii+347 pp. Price £41.25[J]. Elsevier,1987,201(1):373−373.
    [26]
    FIRESTINE D. Official methods and recommended practices of the AOCS/editor of analytical methods[M]. 1998.
    [27]
    LIANG S, YANG G, MA Y. Chemical characteristics and fatty acid profile of foxtail millet bran oil[J]. Journal of the American Oil Chemists Society,2010,87(1):63−67. doi: 10.1007/s11746-009-1475-3
    [28]
    PROESTOS C, BOZIARIS I S, NYCHAS G J E, et al. Analysis of flavonoids and phenolic acids in Greek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity[J]. Food Chemistry,2006,95(4):664−671. doi: 10.1016/j.foodchem.2005.01.049
    [29]
    刘金凯, 高远, 王振宇, 等. 氧化羊骨油脂肪酸组成及挥发性风味物质分析[J]. 现代食品科技,2014,30(11):240−245,169. [LIU J K, GAO Y, WANG Z Y, et al. Analysis of fatty acid composition and volatile flavor compounds in oxidized sheep bone oil[J]. Modern Food Science and Technology,2014,30(11):240−245,169.

    LIU J K, GAO Y, WANG Z Y, et al. Analysis of fatty acid composition and volatile flavor compounds in oxidized sheep bone oil[J]. Modern Food Science and Technology, 2014, 30(11): 240-245, 169.
    [30]
    杨铭铎, 龙志芳, 赵岩, 等. 鸡骨泥的酶解工艺及其酶解液中游离氨基酸和脂肪酸分析的研究[J]. 食品科学,2008(5):162−166. [YANG M D, LONG Z F, ZHAO Y, et al. Study on enzymatic hydrolysis of chicken bone paste and analysis of free amino acids and fatty acids in its hydrolysate[J]. Food Science,2008(5):162−166. doi: 10.3321/j.issn:1002-6630.2008.05.029

    YANG M D, LONG Z F, ZHAO Y, et al. Study on enzymatic hydrolysis of chicken bone paste and analysis of free amino acids and fatty acids in its hydrolysate[J]. Food Science, 2008(5): 162-166. doi: 10.3321/j.issn:1002-6630.2008.05.029
    [31]
    李兰杰, 魏子翔, 张静静, 等. 驴骨、驴皮与阿胶中脂肪酸组成的比较[J]. 现代食品科技,2020,36(4):82−87. [LI L J, WEI Z X, ZHANG J J, et al. The fatty acid composition of donkey, donkey skin and bone gelatin[J]. Modern Food Science and Technology,2020,36(4):82−87.

    LI L J, WEI Z X, ZHANG J J, et al. The fatty acid composition of donkey, donkey skin and bone gelatin[J]. Modern Food Science and Technology, 2020, 36(4): 82-87.
    [32]
    陈银基, 鞠兴荣, 周光宏. 饱和脂肪酸分类与生理功能[J]. 中国油脂,2008(3):35−39. [CHEN Y J, JU X R, ZHOU H G. Classification of saturated fatty acids and the physiological function[J]. China's Oil,2008(3):35−39. doi: 10.3321/j.issn:1003-7969.2008.03.010

    CHEN Y J, JU X R, ZHOU H G. Classification of saturated fatty acids and the physiological function[J]. China's Oil, 2008(3): 35-39. doi: 10.3321/j.issn:1003-7969.2008.03.010
    [33]
    胡燕, 陈忠杰. 不饱和脂肪酸与人体健康关系探讨[J]. 肉类研究,2011,25(1):17−20. [HU Y, CHEN Z J. Unsaturated fatty acid and study on human health[J]. Meat Research,2011,25(1):17−20. doi: 10.3969/j.issn.1001-8123.2011.01.004

    HU Y, CHEN Z J. Unsaturated fatty acid and study on human health[J]. Meat Research, 2011, 25(1): 17-20. doi: 10.3969/j.issn.1001-8123.2011.01.004
    [34]
    谭锦辉, 黄志祥, 李天旺. 多不饱和脂肪酸在骨质疏松症中的研究进展[J]. 临床医学工程,2012,19(7):1218−1220. [TAN J H, HUANG Z X, LI T W. Polyunsaturated fatty acids in the research development of osteoporosis[J]. The Clinical Medical Engineering,2012,19(7):1218−1220. doi: 10.3969/j.issn.1674-4659.2012.07.1218

    TAN J H, HUANG Z X, LI T W. Polyunsaturated fatty acids in the research development of osteoporosis[J]. The Clinical Medical Engineering, 2012, 19(7): 1218-1220. doi: 10.3969/j.issn.1674-4659.2012.07.1218
    [35]
    张志平. n-3多不饱和脂肪酸通过AA代谢通路促进骨折愈合[D]. 广州: 南方医科大学, 2012.

    ZHANG Z P. n-3 Polyunsaturated fatty acids promote fracture healing by AA metabolic pathways[D]. Guangzhou: Southern Medical University, 2012.
    [36]
    杨普煜, 张虹, 刘浚辰, 等. Sn-2棕榈酸酯对婴幼儿健康的促进作用[J]. 食品科学技术学报,2018,36(4):41−45,68. [YANG P Y, ZHANG H, LIU J C, et al. Sn-2 palmitate to infant health[J]. Journal of Food Science and Technology,2018,36(4):41−45,68. doi: 10.3969/j.issn.2095-6002.2018.04.006

    YANG P Y, ZHANG H, LIU J C, et al. Sn-2 palmitate to infant health[J]. Journal of Food Science and Technology, 2018, 36(4): 41-45, 68. doi: 10.3969/j.issn.2095-6002.2018.04.006
    [37]
    陈光华, 黄贵芝, 林瀚, 等. 生育三烯酚通过激活Wnt/β-catenin信号减少激素诱导骨质疏松大鼠骨量流失[J]. 中国骨质疏松杂志,2020,26(11):1618−1622,1684. [CHEN G H, HUANG G Z, LIN H, et al. Fertility triene phenol through the activation of Wnt/β-catenin signaling hormone induced osteoporosis rats bone mass loss reduction[J]. Chinese Journal of Osteoporosis,2020,26(11):1618−1622,1684. doi: 10.3969/j.issn.1006-7108.2020.11.011

    CHEN G H, HUANG G Z, LIN H, et al. Fertility triene phenol through the activation of Wnt/β-catenin signaling hormone induced osteoporosis rats bone mass loss reduction[J]. Chinese Journal of Osteoporosis, 2020, 26(11): 1618-1622, 1684. doi: 10.3969/j.issn.1006-7108.2020.11.011
    [38]
    RIWERO JA, SHAMJI O, KOLOKYTHAS A, 等. 放射性骨坏死: 病理生理学和己酮可可豆碱、α生育酚及氯膦酸盐类药物预防、治疗的回顾[J]. 中国口腔颌面外科杂志,2018,16(1):77. [RIVERO JA, SHAMJI O, KOLOKYTHAS A, et al. Osteoradionecrosis: Pathophysiology and hexanone cocoa alkali chlorine phosphonic acid salts, alpha tocopherol and drug prevention, treatment of the review[J]. Chinese Journal of Oral and Maxillofacial Surgery,2018,16(1):77.

    RIVERO JA, SHAMJI O, KOLOKYTHAS A, et al. Osteoradionecrosis: Pathophysiology and hexanone cocoa alkali chlorine phosphonic acid salts, alpha tocopherol and drug prevention, treatment of the review[J]. Chinese Journal of Oral and Maxillofacial Surgery, 2018, 16(1): 77.
    [39]
    孙曙庆. 油脂氧化稳定性的研究[J]. 食品与发酵工业,1999(3):22−24,37. [SUN Y Q. Study on oxidation stability of oils and fats[J]. Food and Fermentation Industries,1999(3):22−24,37.

    SUN Y Q. Study on oxidation stability of oils and fats[J]. Food and Fermentation Industries, 1999(3): 22-24, 37.
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