Non-targeted Metabolomics Analysis of the Effects of Arabinogalactan on the Metabolites of <i>Akkermansia muciniphila</i>

FENG Xirui; MA Yanshi; WU Xiaoling; KONG Xiangli; ZHANG Tianyang; LOKHOV Dmitrii; SERKOVA Olga; XU Xiaoxi

doi:10.13386/j.issn1002-0306.2021100007

Volume 43 Issue 7

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(7): 21-34. > DOI: 10.13386/j.issn1002-0306.2021100007

FENG Xirui, MA Yanshi, WU Xiaoling, et al. Non-targeted Metabolomics Analysis of the Effects of Arabinogalactan on the Metabolites of Akkermansia muciniphila[J]. Science and Technology of Food Industry, 2022, 43(7): 21−34. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100007.

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Non-targeted Metabolomics Analysis of the Effects of Arabinogalactan on the Metabolites of Akkermansia muciniphila

1.
College of Food Science Key Laboratory of Dairy Science and Education, North East Agricultural University, Harbin 150030, China
2.
Ametis of Russia, Amurskaya Oblast 675000, Russia
3.
Mendeleev Chemical Society of Russia, Moscow 105005, Russia

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Received Date: October 10, 2021
Accepted Date: January 24, 2022
Available Online: February 11, 2022

Graphical Abstract

Abstract

Abstract

Among many plant polysaccharides, arabinogalactan had the best growth-promoting effect on Akkermansia muciniphila (Akk) model strain (DSM 22959). In order to further explore the influence of arabinogalactan on Akk metabolites, arabinogalactan was used as part of the carbon source to ferment Akk in vitro for the first time, and determined the supplementary amount of arabinogalactan to facilitate the Akk fermentation broth. In the subsequent analysis of the samples, high-resolution non-targeted metabolomics technology was used to detect and analyze the differential metabolites in the Akk fermentation broth samples. The results showed that there were 85 different metabolites (VIP>1, P<0.05) and 32 different metabolic pathways. This study confirmed that the arabinogalactan could affect metabolic pathways, biosynthesis of amino acids, 2-oxocarboxylic acid metabolism, ABC transporters and other metabolic pathways, promoting Akk to produce more metabolites with special effects, such as hypoxanthine, mohanone phenol, propionic acid, etc., which had potential of weight loss, anti-cancer and blood pressure lowering, respectively. The results of this study will provide a theoretical basis for the use of arabinogalactan as a prebiotics of Akk or its biogenesis in the future.
- arabinogalactan,
- Akkermansia muciniphila (Akk),
- hypoxanthine,
- mohanone phenol,
- propionic acid,
- metabolic pathways

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