Antioxidant and Anti-aging Activity of <i>Ziziphus jujuba </i>Extract

YE Xiaonan; ZENG Huihui; LIU Siqi; LI Yixiang; HUANG Lili; YU Shuang; LI Tingli; WANG Yanyan

doi:10.13386/j.issn1002-0306.2021090192

Volume 43 Issue 9

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(9): 389-395. > DOI: 10.13386/j.issn1002-0306.2021090192

YE Xiaonan, ZENG Huihui, LIU Siqi, et al. Antioxidant and Anti-aging Activity of Ziziphus jujuba Extract[J]. Science and Technology of Food Industry, 2022, 43(9): 389−395. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090192.

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Antioxidant and Anti-aging Activity of Ziziphus jujuba Extract

1.
Heilongjiang University of Chinese Medicine, Harbin 150040, China
2.
Second Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, China

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Received Date: September 14, 2021
Available Online: March 03, 2022

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Abstract

Abstract

Objective:To illustrate the antioxidant and anti-aging activity of Ziziphus jujuba extract in vivo and in vitro. Methods: The antioxidant activity of Ziziphus jujuba extract (0.05、0.10、0.20、0.40、0.60、0.80、1.00 mg/mL) was determined by DPPH free radical scavenging rate, ·OH radical clearance rate and Fe³⁺ reducing antioxidant power assayed in vitro. Three different doses of Ziziphus jujuba extract (0.1%、0.5%、1.0%) were given to the Drosophila melanogaster through the culture to study the in vivo antioxidant and anti-aging activity by testing the lifespans, negative geotaxis ability, SOD activity, MAD content and CAT activity. Results: The results of in vitro antioxidant activity showed that the Ziziphus jujuba extract could scavenge DPPH and OH free radicals in vitro, and its IC₅₀ were 0.44 and 1.40 mg/mL, respectively. It also could reduce iron in vitro. The in vivo antioxidant and anti-aging activity results showed that the average life span of female and male D. melanogasterwere prolonged by 9.61% and 13.91% in 1.0% Ziziphus jujuba extract group compared with the control group (P<0.01). Also Ziziphus jujuba extract treatment improved the negative geotaxis ability in older female and male D. melanogaster (P<0.05 or P<0.01), decreased the MAD content (P<0.05 or P<0.01) and increased the SOD and CAT activity (P<0.05 or P<0.01). Conclusion: The Ziziphus jujuba extract has good antioxidant and anti-aging capacity in vivo and in vitro.
- Ziziphus jujuba extract,
- antioxidant activity,
- anti-aging,
- Drosophila melanogaster

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References

[1]	尚志钧. 神农本草经辑校[M]. 北京: 学苑出版社, 2014: 41. SHANG Z J. Edited Shennong's herbal classic[M]. Beijing: Xueyuan Press, 2014: 41.
[2]	WU Y, CHEN M, DU M B, et al. Chemical constituents from the fruit of Zizyphus jujuba Mill. var. spinosa[J]. Biochemical Systematics & Ecology,2014,57:6−10.
[3]	田秀红, 黎梅, 张慧姣, 等. 河北酸枣总黄酮提取工艺研究[J]. 食品研究与开发,2015,36(13):76−78. [TIAN X H, LI M, ZHANG H J, et al. Study on the extraction technology of total flavonoids of Hebei wild jujube[J]. Food Research and Development,2015,36(13):76−78. doi: 10.3969/j.issn.1005-6521.2015.13.021
[4]	阮凯华, 唐志书, 刘红波, 等. 不同产地酸枣果肉的品质评价研究[J]. 中南药学,2021,19(5):896−901. [RUAN K H, TANG Z S, LIU H B, et al. Quality evaluation of sour jujube pulp from different origins[J]. Central South Pharmacy,2021,19(5):896−901.
[5]	解玉军, 李泽, 崔小芳, 等. 酸枣化学成分及药理作用研究进展[J]. 中成药,2021,43(5):1269−1275. [XIE Y J, LI Z, CUI X F, et al. Research progress on chemical constituents and pharmacological effects of wild jujube[J]. Chinese Traditional Patent Medicine,2021,43(5):1269−1275. doi: 10.3969/j.issn.1001-1528.2021.05.030
[6]	张蕾, 何晓曦, 徐淑萍, 等. 酸枣果肉的镇静催眠作用[J]. 中药材,2011,34(8):1263−1266. [ZHANG L, HE X X, XU S P, et al. Sedative and hypnotic effects of Zizyphus jujuba pulp[J]. Journal of Chinese Medicinal Materials,2011,34(8):1263−1266.
[7]	郎杰, 崔娜, 张立斌. 酸枣果肉多糖保健功能的初步研究[J]. 西北农林科技大学学报(自然科学版),2014,42(2):162−166. [LANG J, CUI N, ZHANG L B. Preliminary study on healthcare effects of wild jujube pulp polysaccharides[J]. Journal of Northwest A& F University (Nat. Sci. Ed. ),2014,42(2):162−166.
[8]	YUE Y, WU S, ZHANG H, et al. Characterization and hepatoprotective effect of polysaccharides from Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou sarcocarp[J]. Food & Chemical Toxicology,2014,74:76−84.
[9]	薛晓芳, 弓桂花, 赵爱玲, 等. 枣和酸枣不同器官黄酮含量比较及抗氧化能力分析[J]. 中国果树,2020(1):59−63. [XUE X F, GONG G H, ZHAO A L, et al. Comparison of flavonoid contents and antioxidant capacities in different organs of Chinese jujube and wild jujube[J]. Journal of China fruiter,2020(1):59−63.
[10]	王相, 杨梦婷, 高乐, 等. 酸枣不同部位提取物指标成分测定及体外抗氧化活性研究[J]. 现代中医药,2020,40(4):13−19. [WANG X, YANG M T, GAO L, et al. A research on the index component measurement of the extracts fromdifferent parts of wild jujube and their antioxidant activities in vitro[J]. Modern Traditional Chinese Medicine,2020,40(4):13−19.
[11]	施利奇, 张彦青, 戚务勤, 等. 酸枣水提物不同提取工艺优化及抗氧化活性研究[J]. 食品与机械,2019,35(11):182−190. [SHI L Q, ZHANG Y Q, QI W Q, et al. Optimization of different extraction processes of sour jujube juice and study on its antioxidant activity[J]. Food & Machinery,2019,35(11):182−190.
[12]	杜晨晖, 解玉军, 申晨曦, 等. 干燥方式对酸枣果肉活性物质及抗氧化能力的影响[J]. 食品科学,2020,41(15):140−147. [DU C H, XIE Y J, SHENG C X, et al. Effects of different drying methods on bioactive compounds and antioxidant capacity of Ziziphi spinosae sarcocarp[J]. Food Science,2020,41(15):140−147. doi: 10.7506/spkx1002-6630-20190701-012
[13]	解玉军. 基于山西道地中药酸枣的综合利用与功能果酒研发[D]. 太原: 山西中医药大学, 2021. XIE Y J. Comprehensive utilization and functional wine development based on Shanxi genuine traditional Chinese medicine Ziziphus jujube Minn. var. spinosa[D]. Taiyuan: Shanxi University of Chinese Medicine, 2021.
[14]	毛丽衡, 毛永民, 王颉, 等. 酸枣果醋酒精发酵过程工艺的研究[J]. 食品工业科技,2013,34(16):200−202,206. [MAO L H, MAO Y M, WANG J, et al. Study on technology of alcohol fermentation in wild jujube vinegar[J]. Science and Technology of Food Industry,2013,34(16):200−202,206.
[15]	徐蕊, 李祥, 王慧, 等. 酸枣浓缩汁抗氧化和血脂调控的功能评价[J]. 食品研究与开发,2018,39(2):192−197. [XU R, LI X, WANG H, et al. Effect of wild jujube concentrate on antioxidant and lipid metabolism[J]. Food Research and Development,2018,39(2):192−197. doi: 10.3969/j.issn.1005-6521.2018.02.035
[16]	ZHAO Y J, LI J K, ZHANG X, et al. Study on antioxidant activity of flavonoids from leaves of Psidium guajava[J]. China Journal of Chinese Materia Medica,2018,43(4):760−765.
[17]	LUO Y H, LI X R, HE J, et al. Isolation, characterisation, and antioxidant activities of flavonoids from chufa (Eleocharis tuberosa) peels[J]. Food Chem,2014,164:30. doi: 10.1016/j.foodchem.2014.04.103
[18]	SEO J, LEE S, ELAM M L, et al. Study to find the best extraction solvent for use with guava leaves (Psidium guajava L.) for high antioxidant efficacy[J]. Food Sci Nutr,2014,2(2):174. doi: 10.1002/fsn3.91
[19]	陆雨芳, 刘庆庆, 朱成成, 等. 梵净山茶生物活性成分及其抗氧化能力的分析[J]. 食品研究与开发,2021,42(12):46−53. [LU Y F, LIU Q Q, ZHU C C, et al. Analysis of bio-active components and antioxidant capacity of Fanjing Mountain tea[J]. Food Research and Development,2021,42(12):46−53. doi: 10.12161/j.issn.1005-6521.2021.12.008
[20]	GRZEGORCZYK K I, KRZEMINSKA M, KISS A K, et al. Phytochemical profile and antioxidant activity of aerial and underground parts of Salvia bulleyana Diels. plants[J]. Metabolites,2020,10(12):497. doi: 10.3390/metabo10120497
[21]	卞宏生, 蔡江莹, 杨凡静暄. 刺五加总苷及刺五加苷B对持续黑暗条件下果蝇睡眠-觉醒节律的量效关系研究[J]. 中国药物依赖性杂志,2020,29(3):231−236. [BIAN H S, CAI J Y, YANG F J X. Study on the dose-effect relationship of Acanthopanax total glycoside and eleutheroside B onsleep-wake rhythm in Drosophila melanogaster under continuous darkness[J]. Chin J Drug Depend,2020,29(3):231−236.
[22]	秦永燕, 王妤婕, 李颖, 等. 黄芪多糖对果蝇寿命和抗氧化作用的影响[J]. 食品工业科技,2020,41(2):288−291. [QIN Y Y, WANG Y J, LI Y, et al. Effects ofAstragalus polysaccharide on life span and antioxidation of Drosophila melanogaster[J]. Science and Technology of Food Industry,2020,41(2):288−291.
[23]	王艳艳, 翟春梅, 怀雪, 等. 东北刺人参的抗氧化作用研究[J]. 食品研究与开发,2020,41(4):13−18. [WANG Y Y, ZHAI C M, HUAI X, et al. Study of antioxidant activity of Oplopanax elatus Nakai[J]. Food Researchand Development,2020,41(4):13−18.
[24]	刘冀婕, 黄玲艳, 曾照准, 等. 桂花提取物对雄性果蝇抗氧化及抗衰老作用的研究[J]. 食品研究与开发,2018,39(21):38−43. [LIU J J, HUANG L Y, ZENG Z Z, et al. Effects of Osmanthus fragrans alcohol extract on anti-oxidation and anti-aging for male fruit fly[J]. Food Research and Development,2018,39(21):38−43. doi: 10.3969/j.issn.1005-6521.2018.21.007
[25]	HMIDANI A, BOUHLALI E, AJEBLI M, et al. In vitro investigation of antioxidant and antihemolytic activities of three Lamiaceae species from Morocco[J]. Beni-Suef University Journal of Basic and Applied Sciences,2021,10(1):1−8. doi: 10.1186/s43088-020-00091-7
[26]	李萌茹, 周玉枝, 杜冠华, 等. 中药黄酮类化合物抗衰老作用及其机制研究进展[J]. 药学学报,2019,54(8):1382−1391. [LI M R, ZHOU Y Z, DU G H, et al. Research progress about the anti-aging effect and mechanism of flavonoids from traditional Chinese medicine[J]. Acta Pharmaceutica Sinica,2019,54(8):1382−1391.
[27]	TAORMINA G, FERRANTE F, VIENI S, et al. Longevity: Lesson from model organisms[J]. Genes,2019,10(7):518. doi: 10.3390/genes10070518
[28]	王艳艳, 王凤玲, 毛子玮, 等. 具有抗衰老作用中药有效成分及作用机制研究进展[J]. 辽宁中医药大学学报,2021,23(5):142−148. [WANG Y Y, WANG F L. MAO Z W, et al. Advances in research on active ingredients and mechanism of action of traditional Chinese medicine with anti-aging effects[J]. Journal of Liaoning University of TCM,2021,23(5):142−148.
[29]	TOBIASW W, ARIATHNEYC, THOMASE S, et al. Improving mitochondrial function significantly reduces metabolic, visual, motor and cognitive decline in aged Drosophila melanogaster[J]. Neurobiology of Aging,2017,60:34−43. doi: 10.1016/j.neurobiolaging.2017.08.016
[30]	MA C, YANG K, WANG Y, et al. Anti-aging effect of agar oligosaccharide on male Drosophila melanogaster and its preliminary mechanism[J]. Mar Drugs,2019,17(11):632. doi: 10.3390/md17110632
[31]	TANG R, CHEN X, DANG T, et al. Lycium barbarum polysaccharides extend the mean lifespan of Drosophila melanogaster[J]. Food Funct,2019,10(7):4231−4241. doi: 10.1039/C8FO01751D

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