Effect of Incubation Conditions on the Protease Activity and Protein Property of Wheat Germ

ZHANG Yao; SHEN Haijiong; YANG Tianyi; YU Yue; WANG Dongxu; GUO Yuanxin

doi:10.13386/j.issn1002-0306.2021090168

Volume 43 Issue 11

May 2022

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Science and Technology of Food Industry > 2022 > 43(11): 192-199. > DOI: 10.13386/j.issn1002-0306.2021090168

ZHANG Yao, SHEN Haijiong, YANG Tianyi, et al. Effect of Incubation Conditions on the Protease Activity and Protein Property of Wheat Germ[J]. Science and Technology of Food Industry, 2022, 43(11): 192−199. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090168.

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Effect of Incubation Conditions on the Protease Activity and Protein Property of Wheat Germ

1.
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212100, China
2.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

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Received Date: September 12, 2021
Available Online: March 30, 2022

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Abstract

Abstract

Degreased wheat germ, after microwave treatment (600 W, 10 s), was incubated by different conditions for enhancing the activity of its endogenous proteases and increasing the level of polypeptides, including the incubation temperature and time, pH value of incubation solutions and ratio of the solution volume to the wheat germ weight. The results showed that enzymatic activities and polypeptides contents were both significantly affected by the temperature and time, pH value and ratio (P<0.05). A response surface optimization experiment was then conducted by using a Box-Behnken assay, revealing that the highest protease activity and polypeptide level, 3812.34 U/g and 303.12 mg/g, were obtained by a combination of the incubation temperature of 50 ℃ and duration of 6 h, pH of 4.5 and ration of 10:1 mL/g. These results revealed that optimization of culture conditions of wheat germ after degreasing and microwave treatments could effectively enhance the protease activity and polypeptide amount.
- wheat germ,
- endogenous protease,
- incubation,
- protease activity,
- polypeptide content

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References (34)

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