DOU Yanli, CHEN Jin'ao. Effects of Ganoderma lingzhi Polysaccharides Combined with Aerobic Exercise on Lipid-lowering and Antioxidant Levels in Hyperlipidemia Mice[J]. Science and Technology of Food Industry, 2022, 43(9): 372−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090111.
Citation: DOU Yanli, CHEN Jin'ao. Effects of Ganoderma lingzhi Polysaccharides Combined with Aerobic Exercise on Lipid-lowering and Antioxidant Levels in Hyperlipidemia Mice[J]. Science and Technology of Food Industry, 2022, 43(9): 372−380. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090111.

Effects of Ganoderma lingzhi Polysaccharides Combined with Aerobic Exercise on Lipid-lowering and Antioxidant Levels in Hyperlipidemia Mice

More Information
  • Received Date: September 07, 2021
  • Available Online: March 01, 2022
  • To explore the effects of Ganoderma lingzhi fruiting body polysaccharide combined with aerobic exercise on the lipid-lowering and antioxidant levels of high-fat mice. The crude polysaccharide of Ganoderma lingzhi fruit body was extracted by water extraction and alcohol precipitation, and then purified by deproteinization, separation and purification to obtain pure Ganoderma lingzhi polysaccharide (GLP). The structural characteristics of GLP were analyzed by scanning electron microscope and Fourier infrared spectroscopy. A high-fat mouse model was constructed by feeding high-fat diets, including normal feeding group (CG), high-fat diet group (HD), Ganoderma lingzhi polysaccharide group (GLP), Aerobic exercise control group (AE) and Ganoderma lingzhi polysaccharide and aerobic exercise combined group (GLP/AE), GLP group and GLP/AE group were given Ganoderma lingzhi polysaccharide gavage at a dose of 2.0 g/kg/d, and the rest groups were given the same amount of normal saline, and the AE group and GLP/AE group were given aerobic treadmill exercise, 1 h/d, 5 d a week. After 8 weeks of aerobic treadmill training, the concentrations of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in the serum of each group of mice were measured. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxide dismutase (GSH-Px) and malondialdehyde (MDA) contents, and pathological observations were made on the liver tissues of mice in each group. The result showed Ganoderma lingzhi polysaccharide presented an irregular sheet structure, the surface was not smooth, there were spot-like protrusions and depressions, and its anomeric carbon was in the β configuration. After 8 weeks of high-fat diet, the weight of the HD group increased by 32.95% and the liver weight increased by 40.19% compared with the CG group. TG, TC, and LDL-C all increased significantly, while the endogenous resistance The activity of the oxidase system was significantly reduced, indicating that the oxidative stress caused by lipid peroxidation caused damage to the mice. The atherosclerosis index of the HD group was significantly higher than that of the CG group (P<0.05); GLP, AE and GLP/AE group, the serum levels of TC, TG, and LDL-C in mice were significantly reduced, while the content of HDL-C increased. Among them, aerobic exercise combined with Ganoderma lingzhi polysaccharide intervention could significantly reduce blood lipids, reducing the content of TG, TC and LDL-C by 55.79%, 22.54% and 62.52%, respectively. In addition, compared with the HD group, the Ganoderma lingzhi polysaccharide group, aerobic exercise group, and Ganoderma lingzhi polysaccharide combined with aerobic exercise group could reshape the activity of endogenous antioxidant enzymes, clear MDA, and reduce lipid droplets in liver cells and the degree of fatty degeneration. It could be seen that the combination of aerobic exercise and supplementation of Ganoderma lingzhi polysaccharides had a significant effect on lowering blood lipids in mice, and at the same time can enhance the resistance of mice to oxidative damage caused by lipid peroxidation. Ganoderma lingzhi polysaccharide was expected to be developed as a weight loss and lipid-lowering product. Ganoderma lingzhi polysaccharide combined with aerobic exercise intervention was a potential way to prevent and assist the treatment of hyperlipidemia.
  • [1]
    杨栩, 纪海玉, 于娟, 等. 一种新型复合多糖提取优化和降血脂活性的研究及其胆固醇结合力预测模型的建立[J]. 食品安全质量检测学报,2021,12(10):4026−4033. [YANG X, JI H Y, YU J, et al. Study on the extraction optimization and hypolipidemic activities of a novel compound polysaccharides and the establishment of prediction model of cholesterol-binding capacity[J]. Journal of Food Safety and Quality,2021,12(10):4026−4033.
    [2]
    王倩倩, 王晓航, 邱山虎, 等. 运动对肥胖相关代谢异常的作用[J]. 中国实用内科杂志,2022,42(2):102−106. [WANG Q Q, WANG X H, QIU S H, et al. Effects of exercise on obesity-related metabolic abnormalities[J]. Chinese Journal of Practical Internal Medicine,2022,42(2):102−106.
    [3]
    梁有峰, 郭增, 程宝山, 等. 非高密度脂蛋白胆固醇与载脂蛋白A-I比值与冠状动脉慢血流相关性分析[J]. 中国心血管病研究,2022,20(2):161−165. [LIANG Y F, GUO Z, CHENG B S, et al. Correlation analysis between non-high-density lipoprotein cholesterol and apolipoprotein A-I ratio and coronary slow blood flow[J]. Chinese Cardiovascular Research,2022,20(2):161−165.
    [4]
    ZHAO L Y, HUANG W, YUAN Q X, et al. Hypolipidaemic effects and mechanisms of the main component of Opuntia dillenii Haw. polysaccharides in high-fat emulsion-induced hyperlipidaemic rats[J]. Food Chem,2012,134(2):964−971. doi: 10.1016/j.foodchem.2012.03.001
    [5]
    JIANG C, WANG Q, WEI Y J, et al. Cholesterol-lowering effects and potential mechanisms of different polar extracts from Cyclocarya paliurus leave in hyperlipidemic mice[J]. J Ethnopharmacol,2015,176(March):17−26.
    [6]
    MENG L Z, XIE J, LV G P, et al. A comparative study on immunomodulatory activity of polysaccharides from two official species of Ganoderma (Lingzhi)[J]. Nutr Cancer,2014,66(7):1124−1131. doi: 10.1080/01635581.2014.948215
    [7]
    CAO Y, YUAN H S. Ganoderma mutabile sp. nov. from southwestern China based on morphological and molecular data[J]. Mycol Prog,2013,12(1):121−126.
    [8]
    戴玉成, 曹云, 周丽伟, 等. 中国灵芝学名之管见[J]. 菌物学报,2013,32(6):947−952. [DAI Y C, CAO Y, ZHOU L W, et al. Notes on the nomenclature of the most widely cultivated Ganoderma species in China[J]. Mycosystema,2013,32(6):947−952.
    [9]
    GOWRIE S U, CHATHURDEVI G, RANI K. Evaluation of bioactive potential of basidiocarp extracts of Ganoderma lucidum[J]. Int J Pharm Res All Sci,2014,3(1):36−46.
    [10]
    周鸣. 有氧运动对肥胖大学生部分心血管疾病危险因素的影响[D]. 南昌: 华东交通大学, 2017.

    ZHOU M. The effect of aerobic exercise on some risk factors of cardiovascular disease in obese college students[D]. Nanchang: East China Jiaotong University, 2017.
    [11]
    MEYER A A, KUNDT G, LENSCHOW U, et al. Improvement of early vascular changes and cardiovascular risk factors in obese children after a six-month exercise program[J]. J Am Coll Cardiol,2006,48(9):1865−1870. doi: 10.1016/j.jacc.2006.07.035
    [12]
    高思垚, 吕万刚, 聂应军. 不同运动方式对中国超重或肥胖人群代谢指标影响的元分析[J]. 中国体育科技,2021,57(10):46−54,69. [GAO S Y, LV W G, NIE Y J. Meta-analysis of the effects of different exercise methods on metabolic indicators in Chinese overweight or obese people[J]. China Sports Science and Technology,2021,57(10):46−54,69.
    [13]
    张蕾, 王瑛, 朱惠照, 等. 赤灵芝新菌株“仙芝2号”的选育[J]. 食用菌学报,2014,21(1):15−20. [ZHANG L, WANG Y, ZHU H Z, et al. Breeding of a new strain of Ganoderma lucidum "Xianzhi 2"[J]. Acta Edible Fungi,2014,21(1):15−20. doi: 10.3969/j.issn.1005-9873.2014.01.003
    [14]
    熊川, 罗强, 金鑫, 等. 人工栽培灵芝中多糖的部分理化性质及免疫调节作用[J]. 微生物学通报,2018,45(4):825−835. [XIONG C, LUO Q, JIN X, et al. Physiochemical properties immunoregulatory effects of the polysaccharides from Ganodema lingzhi[J]. Microbiol China,2018,45(4):825−835.
    [15]
    孙恬, 郭爱玲. 蓝白光膜对灵芝田间生长、多糖含量及三萜含量的影响[J]. 生物化工,2019,5(1):70−72,76. [SUN T, GUO A L. Effect of blue and white light film on the field growth, polysaccharide content and triterpene content of Ganoderma lucidum[J]. Biochemical Industry,2019,5(1):70−72,76.
    [16]
    秦利鸿, 曹建波, 易伟松. 绿茶多糖的扫描电镜制样新方法及原子力显微镜观察[J]. 电子显微学报,2009,28(2):162−167. [TAI L H, CAO J B, YI W S. Polysaccharides distilled from green tea and the observation of ultrafine structure of them with atomic force microscope[J]. J Chin Electron Microsc Soc,2009,28(2):162−167. doi: 10.3969/j.issn.1000-6281.2009.02.014
    [17]
    彭天祥, 张娟, 张利, 等. 梯棱羊肚菌多糖的理化性质及神经保护活性研究[J]. 现代食品科技,2019,35(9):87−95. [PENG T X, ZHANG J, ZHANG L, et al. Study on the physicochemical properties and neuroprotective activity of polysaccharides from Morchella typhimurium[J]. Mod Food Sci Techno,2019,35(9):87−95.
    [18]
    曹智. 翅果油联合有氧运动对高脂饮食小鼠的降脂减肥作用[D]. 太原: 山西大学, 2019.

    CAO Z. Effect of decreasing obesity and lipid-lowering of Elaeagnus mollis oil with aerobic exercise in high fat diet-fed mice[D]. Taiyuan: Shanxi University, 2019.
    [19]
    张军, 段杉, 全丽金, 等. 连续相变萃取灵芝多糖动力学球状模型的建立及其结构特征[J]. 食品工业科技,2020,41(17):114−120. [ZHANG J, DUAN S, QUAN L J, et al. Establishment of a kinetic spherical model of Ganoderma lucidum polysaccharide extraction by continuous phase change and its structural characteristics[J]. Sci Techno Food Ind,2020,41(17):114−120.
    [20]
    金鑫, 熊川, 李萍, 等. 三株海南岛野生灵芝的鉴定、多糖组成及其抗氧化活性研究[J]. 天然产物研究与开发,2020,32(2):190−199. [JIN X, XIONG C, LI P, et al. Identification, polysaccharide composition and antioxidant activity of three wild Ganoderma lucidum from Hainan Island[J]. Natural Products Res Dev,2020,32(2):190−199.
    [21]
    冯峰, 杨淑玲, 张奇, 等. 霸王花不同部位粗多糖的结构组成及体外抗氧化活性[J]. 中国现代应用药学,2021,38(2):189−195. [FENG F, YANG S L, ZHANG Q, et al. Structural composition and in vitro antioxidant activity of crude polysaccharides from different parts of Polygonum sibiricum[J]. The Chinese Journal of Modern Applied Pharmacy,2021,38(2):189−195.
    [22]
    冯蒙蒙, 王海鸣, 杨惠成, 等. 灵芝孢子粉多糖的分离纯化、结构表征及免疫活性初探[J]. 食品与生物技术学报,2018,37(5):502−508. [FENG M M, WANG H M, YANG H C, et al. Isolation, purification, structural characterization and preliminary study of immunological activity of polysaccharides from Ganoderma lucidum spore powder[J]. Journal of Food and Biotechnology,2018,37(5):502−508. doi: 10.3969/j.issn.1673-1689.2018.05.008
    [23]
    LIU W, LV X, HUANG W H, et al. Characterization and hypoglycemic effect of a neutral polysaccharide extracted from the residue of Codonopsis pilosula[J]. Carbohydrate Polymers,2018,198:215−226. doi: 10.1016/j.carbpol.2018.06.018
    [24]
    GUO L, XIE J H, RUAN Y Y, et al. Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum[J]. International Immunopharmacology,2009,9:1175−1182. doi: 10.1016/j.intimp.2009.06.005
    [25]
    TUNNICLIFFE J M, ELLER L K, REIMER R A, et al. Chlorogenic acid differentially affects postprandial glucose and glucose-dependent insulinotropic polypeptide response in rats[J]. Appl Physiol Nutr Metab,2011,36(5):650−659.
    [26]
    陈娟, 汪胡风, 邓军, 等. 丹红注射液对高脂血症大鼠肝脏AMPK/SREBP-1/ACC通路的影响[J]. 中国药理学通报,2018,34(4):528−532. [CHEN J, WANG H F, DENG J, et al. Effect of Danhong injection on AMPK/SREBP-1/ACC pathways of liver in hyperlipidemic rats[J]. Chin Pharmacol Bull,2018,34(4):528−532. doi: 10.3969/j.issn.1001-1978.2018.04.018
    [27]
    于航, 杨志洛, 张宁, 等. 外周动脉斑块与冠状动脉粥样硬化程度的相关性及其预测价值[J]. 中国循证心血管医学杂志,2021,13(7):847−850. [YU H, YANG Z L, ZHANG N, et al. Correlation between peripheral arterial plaque and coronary atherosclerosis and its predictive value[J]. China Journal of Evidence-Based Cardiovascular Medicine,2021,13(7):847−850. doi: 10.3969/j.issn.1674-4055.2021.07.20
    [28]
    GERACI G, ZAMUTO M, GAETANI R, et al. Relationship of a body shape index and body roundness index, with carotid atherosclerosis in arterial hypertension[J]. Nutrmetabcardiovas,2019,29(8):822−829.
    [29]
    MORRONE M, ASSIS A M D, ROCHA R, et al. Passiflora manicata (Juss.) aqueous leaf extract protects against reactive oxygen species and protein glycation in vitro and ex vivo models[J]. Food ChemToxicol,2013,60:45−51. doi: 10.1016/j.fct.2013.07.028
    [30]
    ZHU S, ZHAO L, FAN Y, et al. Interaction between TNF-α and oxidative stress status in first-episode drug-nave schizophrenia[J]. Psychoneuroendocrino,2020,114:104595. doi: 10.1016/j.psyneuen.2020.104595
    [31]
    FLEMMING N, BORG M B, BO N J, et al. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors[J]. Clin Chem,1997(7):1209−1214.
    [32]
    MURASE T, NAGASAWA A, SUZUKI J, et al. Beneficial effects of tea catechins on diet-induced obesity: Stimulation of lipid catabolism in the liver[J]. Int J Obes Relat Metab Disord,2002,26(11):1459−1464.
    [33]
    张群豪, 林志彬. 灵芝多糖 (GL-B) 对肿瘤坏死因子αγ干扰素产生及其mRNA表达的影响[J]. 北京医科大学学报,1999,31(2):179−183. [ZHANG Q H, LIN Z B. Effect of Ganoderma lingzhi polysaccharides B on TNFα and IFNγ production and their mRNA expression[J]. J Beijing Med Univ,1999,31(2):179−183.
  • Cited by

    Periodical cited type(35)

    1. 李秀清,何江龙,李盼盼,纪宝玉,裴莉昕,陈随清,董诚明. 麦冬不同部位红外光谱分析及成分含量的测定. 饲料工业. 2025(03): 112-117 .
    2. 罗欣,胡杨,倪兴婷,谈小飞,黄涅,赖富丽,李强,杨强. 九蒸九制对九华山多花黄精品质的影响. 中国酿造. 2025(02): 144-149 .
    3. 袁怡菁,王秋红. 黄精化学成分、药理作用研究进展及质量标志物预测分析. 中医药信息. 2024(02): 72-80+86 .
    4. 张倜培,李青,包鸿慧,沈正兴,石娟,唐前勇,程一方,周睿. 干燥方式对绿碎茶多酚提取物体外抗氧化和抗糖尿病及乙酰胆碱酯酶抑制活性的影响. 食品与发酵工业. 2024(04): 77-84 .
    5. 罗亚惠,宿珠琳,马永生,邹建. 超声辅助法提取柚子皮多酚的工艺优化. 现代食品. 2024(01): 51-56 .
    6. 黄素艳,崔柯鑫,周德庆,王珊珊,王明丽,王大军. 植物多酚改善肠道屏障及糖脂代谢的研究进展. 食品研究与开发. 2024(06): 218-224 .
    7. 关洋莹,唐守方,彭雨鑫,李梦媛,刘赢心,蒋璥远,冯淑婷,张新民,武毅. 桦褐孔菌抗糖尿病的研究进展. 长春中医药大学学报. 2024(04): 463-467 .
    8. 王爱灵,张梅,申娜娜,徐梦文,章骏伟,李瑞龙,兰伟. 黑果腺肋花楸果酒生产工艺研究进展. 滁州学院学报. 2024(02): 21-28 .
    9. 符兵,周东来,李庆荣,邢东旭. 桑叶及其活性物质对动物肠道微生物的调控作用研究进展. 中国畜牧兽医. 2024(06): 2460-2470 .
    10. 王菁,乔勇进,柳洪入,王晓,叶章颖,林思敏,王春芳. 超高压在植物活性物质提取中的应用研究进展. 农业工程学报. 2024(18): 272-282 .
    11. 陈睿,李佳轩,王旭,谢双龙,覃继肖,许一清,卢琦,田兴舟. 刺梨籽对肉兔消化代谢、血浆生化指标、屠宰性能及肉品质的影响. 动物营养学报. 2024(10): 6673-6684 .
    12. 郭子斌,魏静,范蓓,王凤忠,蔡如玉,孙晶. 辅助降血糖茄皮含片制备. 热带农业科学. 2024(08): 65-74 .
    13. 薛惠丹,张未希,邓诏隆,董瑞杰,陈瑞飞,安靖. 徐香猕猴桃果皮多酚提取工艺的优化及其单体抗氧化活性研究. 陕西科技大学学报. 2024(06): 31-39 .
    14. 那治国,余爽,贺书珍,初众. 低GI杂粮可可冲调粉辅助降血糖作用. 食品工业科技. 2023(01): 28-37 . 本站查看
    15. 陈燕萌,魏金兰,陈凤,黄宇扬,李珊珊,郭松. 假蒟叶多酚提取工艺及其抗氧化活性研究. 中国食品添加剂. 2023(02): 76-84 .
    16. 邱建强,黄文,王玉玺,彭凯. 单宁对动物肠道微生物调控的研究进展. 动物营养学报. 2023(02): 772-781 .
    17. 陈晓晨,杜希萍,周莉鹃,伍菱,李志朋,杨远帆. 坛紫菜多酚工艺优化及降血糖和抗氧化活性研究. 食品与机械. 2023(02): 140-146 .
    18. 周爽,黄莉,王彦美,张韩杰,赵莉,贺子阳,孙素洁. 植物多酚的生物利用度及其降血糖活性研究进展. 食品研究与开发. 2023(06): 211-217 .
    19. 沈晓静,袁文娟,邵俊文,文午,赵品材,洪金琪,顾春燕,姜薇薇. 云南小粒咖啡生豆的抗氧化及α-淀粉酶抑制活性研究. 中国食品添加剂. 2023(05): 96-102 .
    20. 师英春,廖森泰,杨琼,邹宇晓,李倩. 植物多酚和多糖经肠道微生态途径互作调节糖脂代谢的研究进展. 食品安全质量检测学报. 2023(08): 109-118 .
    21. 杨淑珺,胡睿智,贺建华,伍树松. 植物多酚对脂肪酸代谢的调控作用及潜在机制. 中国畜牧杂志. 2023(07): 14-20 .
    22. 史凡,杨红. 纳米结构脂质载体制备及在功能食品中的应用研究进展. 食品工业科技. 2023(15): 1-6 . 本站查看
    23. 陈鑫,王晨,王黎明,赵抒娜,赵芸,刘孟涛,孟庆佳. L-阿拉伯糖复配糖对小鼠血糖的影响. 食品工业科技. 2023(17): 392-398 . 本站查看
    24. 李琳琳,王乐,尹卫,代爽,刘晓军,梁健,王煜伟. 紫皮大蒜多酚分离纯化及体外降血糖活性研究. 中国调味品. 2023(09): 18-23 .
    25. 王露,黄立新,刘磊. 食源性多酚对α-淀粉酶作用大米淀粉活性的影响. 中国粮油学报. 2023(08): 131-137 .
    26. 唐双庆,陈禅友,王亚珍,王红波. 食用豆类资源中酚类化合物研究进展. 中国调味品. 2023(12): 200-205+212 .
    27. 徐小云,伍舒华,赖敏成,廖延智,吴文,欧纯宜. 高效液相色谱测定龙眼核中多酚含量方法优化. 广州化工. 2023(22): 61-64 .
    28. 彭言劼,魏雪莲,周勇,胡强,胡霞,宋春草. 不同果袋对红富士葡萄果实品质的影响. 中国南方果树. 2022(02): 134-138 .
    29. 陈旭,徐楚炎,范露,石玉. 山楂叶多酚提取工艺及其降糖降脂应用研究. 饲料研究. 2022(05): 78-83 .
    30. 滕欢欢,王仁中,吴德玲,金传山,柳春风,唐旭,黄圣卓,许凤清. 多花黄精炮制前后不同极性部位抗氧化与降血糖活性研究. 食品与发酵工业. 2022(08): 70-75 .
    31. 吴卫成,忻晓庭,张程程,刘大群,卢立志,胡宏海,章检明,张治国,郭阳. 番薯叶多酚提取工艺优化及其生物活性研究. 中国食品学报. 2022(05): 189-199 .
    32. 胡德胜,李健,张岩,钟丹苗,方雪飞,廖营忠,苏干光. 林下栽培的大球盖菇多酚提取工艺条件优化. 中国食用菌. 2022(08): 65-70 .
    33. 张杰,党斌,杨希娟. 植物多酚的生理活性、抑菌机理及其在食品保鲜中的应用研究进展. 食品工业科技. 2022(24): 460-468 . 本站查看
    34. 马雪,赵丹,张瑞,琚艳君,赵多勇. 多酚类化合物检测分析方法研究进展. 食品安全质量检测学报. 2021(11): 4575-4582 .
    35. 高若容,王钿烈,黄雪松. 固相萃取-高效液相色谱法同时测定蒜皮中六种酚类物质. 食品与发酵工业. 2021(22): 266-272 .

    Other cited types(23)

Catalog

    Article Metrics

    Article views (165) PDF downloads (23) Cited by(58)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return