Effect of Adding Glutamine Transaminase on the Quality of Set-style Yogurt

HUO Chenchen; HU Zhihe; LU Dingqiang; ZHU Yuting; XIAO Houdong; YI Wan; LI Mingrui

doi:10.13386/j.issn1002-0306.2021090097

Volume 43 Issue 11

May 2022

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Science and Technology of Food Industry > 2022 > 43(11): 83-95. > DOI: 10.13386/j.issn1002-0306.2021090097

HUO Chenchen, HU Zhihe, LU Dingqiang, et al. Effect of Adding Glutamine Transaminase on the Quality of Set-style Yogurt[J]. Science and Technology of Food Industry, 2022, 43(11): 83−95. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090097.

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Effect of Adding Glutamine Transaminase on the Quality of Set-style Yogurt

Tianjin Key Laboratory of Food and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

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Received Date: September 07, 2021
Available Online: March 29, 2022

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Abstract

Abstract

The objective of this work was to study the difference of physical and chemical indexes and sensory indexes between set-style yogurt with TG enzyme and plain set-style yogurt (without TG enzyme) in the fermentation process. The changes of acidity, viscosity, hardness, water holding capacity and viable count of yogurt during fermentation were measured by phenolphthalein indicator method, rheometer, texture analyzer, centrifugation and plate counting method, respectively. The changes of color, taste and smell were measured by electronic eye, electronic tongue and electronic nose, respectively, and sensory evaluation was carried out. The results showed that adding 0~5 U TG enzyme per gram of milk protein, the acidity of yogurt at the end of fermentation was within the range of 71.12~73.64 ^oT, and there was no significant difference in the acidity of yogurt (P>0.05). During the fermentation process, the viscosity and hardness increased significantly after 3 h (P<0.05), and the viscosity and hardness of set-style yogurt with TG enzyme increased significantly at the end of fermentation (P<0.05). With the increase of TG enzyme content, the water holding capacity increased significantly (P<0.05). Adding different concentrations of TG enzyme had no significant effect on the number of viable bacteria in set-style yogurt (P>0.05). The main color number of TG enzyme set-style yoghurt and common set-style yoghurt were both 4095, and there was no significant difference in color at the end of fermentation (P>0.05). By principal component analysis, adding different concentrations of TG enzyme after fermentation had no significant effect on the taste and smell of yogurt (P>0.05). According to sensory evaluation, yogurt that 1 g milk protein supplemented with 2 U TG enzyme had the best sensory. Therefore, compared with the plain set-style yogurt without TG enzyme, the coagulated yogurt with TG enzyme was not significantly different in acidity, live bacteria count, color, taste and odor (P>0.05); it was significantly higher in viscosity, hardness and water-holding capacity (P<0.05), and the addition of appropriate amount of TG enzyme was beneficial to the improvement of the quality of coagulated yogurt.
- set-style yoghurt,
- glutamine transaminase,
- physical and chemical indicators,
- sensory index,
- comparison of differences

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References

[1]	CÁCERES P J, PEÑAS E, MARTÍNEZ-VILLALUENGA C, et al. Development of a multifunctional yogurt-like product from germinated brown rice[J]. LWT,2019,99:306−312. doi: 10.1016/j.lwt.2018.10.008
[2]	贾迪, 吴秀英, 李树森, 等. 谷氨酰胺转氨酶在乳制品中应用的研究进展[J]. 食品科技,2019,44(5):14−18. [JIA Di, WU Xiuying, LI Shusen, et al. Research progress on the application of transglutaminase in dairy products[J]. Food Science and Technology,2019,44(5):14−18.
[3]	李寅. “微生物发酵法生产谷氨酰胺转胺酶”通过鉴定[J]. 工业微生物,2000(4):44. [LI Yin. "Production of transglutaminase by microbial fermentation" passed the appraisal[J]. Industrial Microorganism,2000(4):44.
[4]	ONER Z, KARAHAN A G, AYDEMIR S, et al. Effect of transglutaminase on physicochemical properties of set-style yogurt[J]. International Journal of Food Properties,2008,11(1):196−205. doi: 10.1080/10942910701286346
[5]	DEMIRKAYA A K, CEYLAN Z G. The effect of microbial transglutaminase on microbiological, chemical, textural and sensory properties of yogurt[J]. Australian Journal of Dairy Technology,2009,64(2):171−176.
[6]	ANLI T, SEZGIN E, DEVECI O, et al. Effect of using transglutaminase on physical, chemical and sensory properties of set-type yoghurt[J]. Food Hydrocolloids,2010,25(6):1477−1481.
[7]	JOOYANDEH H, MAHMOODI R, HOJJATI M. Effect of cold enzymatic treatment of milk by transglutaminase on textural properties of yogurt[J]. 2015, 13(1): 23.
[8]	PAVUNC AL, BEGANOVIĆ J, KOS B, et al. Influence of microencapsulation and transglutaminase on viability of probiotic strain Lactobacillus helveticus M92 and consistency of set yoghurt[J]. 2011, 64(2) : 254−261.
[9]	SHIEIKIN A G, ZIPAEV D V, ZHILINSKAYA N T, et al. Structure properties of stirred yoghurt made with transglutaminase and Amaranth[J]. Carpathian Journal of Food Science & Technology,2016,8(2):71−80.
[10]	ABDRABO F H R, ELDIEB S M, ABDELFATTAH A M, et al. Characteristics of set-style yoghurt manufactured from transglutaminase treated milk[J]. Egyptian Journal of Dairy Science,2014,42(1):37−50.
[11]	NOGHANI V F, MOFIDI A, ZAREI M. Effect of using microbial transglutaminase as a substitute for part of milk protein concentrate on the selected physicochemical and sensory properties of spinach yoghurt[J]. Iranian Journal of Nutrition Sciences & Food Technology,2014,9(3):93−100.
[12]	FADAEI N V, MOFIDI A, ZAREI M. Using of microbial transglutaminase as a substitute for part of skim milk powder in spinach yoghurt[J]. 2016, 13(1): 17−24.
[13]	LORENZEN P C, NEVE H, MAUTNER A, et al. Effect of enzymatic cross-linking of milk proteins on functional properties of set-style yoghurt[J]. Interational Joural of Dairy Technology,2002,55(3):152−157. doi: 10.1046/j.1471-0307.2002.00065.x
[14]	ROMEIH EA, ABDEL-HAMID M, AWAD A A. The addition of buttermilk powder and transglutaminase improves textural and organoleptic properties of fat-free buffalo yogurt[J]. Dairy Science & Technology,2014,94(3):297−309.
[15]	YÜKSEL Z, ERDEM Y K. The influence of transglutaminase treatment on functional properties of set yoghurt[J]. International Journal of Dairy Technology,2010,63(1):86−97. doi: 10.1111/j.1471-0307.2009.00539.x
[16]	PARAMBAN R M, KUMAR R, MANN B, et al. Enzymatic modification of milk proteins for the preparation of low fat dahi[J]. Journal of Food Processing & Preservation,2016,40(5):1038−1046.
[17]	金洪伟, 张毅, 高源, 等. 谷氨酰胺转胺酶的功能性质及在乳制品中的应用[J]. 中国乳品工业,2013,41(2):37−40. [JIN Hongwei, ZHANG Yi, GAO Yuan, et al. Fuctions of transglutaminase and its application in dairy product[J]. China Dairy Industry,2013,41(2):37−40. doi: 10.3969/j.issn.1001-2230.2013.02.010
[18]	李思宁, 唐善虎, 毛濛兰, 等. TG酶和漆酶对双歧杆菌益生菌酸奶品质的影响[J]. 食品工业科技,2018,39(8):119−125, 135. [LI Sining, TANG Shanhu, MAO Menglan, et al. Effect of TGase and laccase on the quality of probiotics yogurt[J]. Science and Technology of Food Industry,2018,39(8):119−125, 135.
[19]	LI H B, LI C S, LIU T T, et al. Textural performance of Zea mays transglutaminase modified milk protein concentrate in stirred yoghurt[J]. LWT, 2021, 147(S1): 111625.
[20]	杨洋, 刘奕伟, 周方, 等. TG酶与稳定剂复配改善搅拌型酸奶生产特性的研究[J]. 中国乳品工业,2013,41(4):26−31. [YANG Yang, LIU Yiwei, ZHOU Fang, et al. Applications of transglutaminase plus a stabilizer in improving the processing characteristics of stirred yogurt product[J]. China Dairy Industry,2013,41(4):26−31. doi: 10.3969/j.issn.1001-2230.2013.04.007
[21]	王银. 谷氨酰胺转氨酶对酸羊乳凝乳特性及贮存期品质的影响[D]. 西安: 陕西师范大学, 2018. WANG Yin. Effect of glutamine transaminase on curd characteristics and storage quality of sour goat milk[D]. Xi’an: Shaanxi Normal University, 2018.
[22]	李洪波, 张君颜, 李卿, 等. 谷氨酰胺转氨酶在酸奶中的应用研究[J]. 安徽农业科学,2017,45(18):86−88. [LI Hongbo, ZHANG Junyan, LI Qing, et al. Application of transglutaminase in yoghurt[J]. Journal of Anhui Agricultural Sciences,2017,45(18):86−88. doi: 10.3969/j.issn.0517-6611.2017.18.027
[23]	张莉丽, 韩雪, 张兰威, 等. 茂原链霉菌谷氨酰胺转氨酶在酸奶中的应用研究[J]. 食品工业科技,2014,35(12):210−212, 217. [ZHANG Lili, HAN Xue, ZHANG Lanwei, et al. Application of transglutaminase from Streptomyces mobaraense in yoghurt[J]. Science and Technology of Food Industry,2014,35(12):210−212, 217.
[24]	郭颖. 谷氨酰胺转氨酶对酸羊乳凝胶特性的影响分析[J]. 中国乳业,2020(7):70−72. [GUO Ying. Effect of glutamine transaminase on gel properties of sour goat milk[J]. China Dairy,2020(7):70−72.
[25]	DOMAGAłA J, WSZOłEK M, TAMIME A Y, et al. The effect of transglutaminase concentration on the texture, syneresis and microstructure of set-type goat's milk yoghurt during the storage period[J]. Small Ruminant Research,2013,112(1-3):154−161. doi: 10.1016/j.smallrumres.2012.12.003
[26]	张艳, 张富新, 王毕妮, 等. 谷氨酰胺转氨酶浓度对巴氏杀菌酸羊奶品质的影响[J]. 食品与发酵工业,2019,45(7):201−206. [ZHANG Yan, ZHANG Fuxin, WANG Bini, et al. Effects of transglutaminase concentration on quality of pasteurized goat yogurt[J]. Food and Fermentation Industries,2019,45(7):201−206.
[27]	王文超, 袁海娜, 赵广生, 等. 谷氨酰胺转氨酶对酸奶乳清蛋白抗氧化活性的影响[J]. 中国食品学报,2017,17(8):80−85. [WANG Wenchao, YUAN Haina, ZHAO Guangsheng, et al. Effect of transglutaminase on antioxidant activity of whey protein in yoghurt[J]. Journal of Chinese Institute of Food Science and Technology,2017,17(8):80−85.
[28]	吴彤, 姜淑娟, 妥彦峰, 等. 热处理和转谷氨酰胺酶对凝固型酸乳品质的影响[J]. 食品工业科技,2020,41(18):33−37. [WU Tong, JIANG Shujuan, TUO Yanfeng, et al. Effects of heat treatment and transglutaminase on the quality of set yoghurt[J]. Science and Technology of Food Industry,2020,41(18):33−37.
[29]	ZHANG L, ZHANG L, YI H, et al. Enzymatic characterization of transglutaminase from Streptomyces mobaraensis DSM 40587 in high salt and effect of enzymatic cross-linking of yak milk proteins on functional properties of stirred yogurt[J]. Journal of Dairy Science,2012,95(7):3559−3568. doi: 10.3168/jds.2011-5125
[30]	张春红, 孙婧, 许娇, 等. 利用固定化谷氨酰胺转氨酶生产搅拌型软枣猕猴桃果肉酸奶[J]. 食品与发酵工业,2011,37(5):209−212. [ZHANG Chunhong, SUN Jing, XU Jiao, et al. Research of immobilized transglutaminase in stirred actinidia arguta fruit yogurt[J]. Food and Fermentation Industries,2011,37(5):209−212.
[31]	GAUCHE C, TOMAZI T, BARRETO PLM, et al. Physical properties of yoghurt manufactured with milk whey and transglutaminase[J]. LWT-Food Science and Technology,2008,42(1):239−243.
[32]	TSEVDOU M S, ELEFTHERIOU E G, TAOUKIS P S, et al. Transglutaminase treatment of thermally and high pressure processed milk: Effects on the properties and storage stability of set yoghurt[J]. Innovative Food Science and Emerging Technologies,2013,17:144−152. doi: 10.1016/j.ifset.2012.11.004
[33]	FARGEMAND M, SORENSEN M V, JORGENSEN U, et al. Transglutaminase: Effect on instrumental and sensory texture of set style yoghurt[J]. Milchwissenschaft-milk Science Intemational,1999,54(10):563−566.
[34]	MOTOKI M, SEGURO K. Transglutaminase and its use for food processing[J]. Trends in Food Science & Technology,1998,9(5):204−210.
[35]	白丽娟, 张英龙, 刘青云, 等. 响应面法优化凝固型羊奶酸奶生产工艺[J]. 中国乳品工业,2020,48(1):56−60. [BAI Lijuan, ZHANG Yinglong, LIU Qingyun, et al. Optimized the production process of solidified goat’s milk yogurt[J]. China Dairy Industry,2020,48(1):56−60.
[36]	中华人民共和国国家卫生和计划生育委员会. GB 5009.239-2016食品安全国家标准食品酸度的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the PRC. GB 5009.239-2016 National standard for food safety determination of acidity of food[S]. Beijing: Standards Press of China, 2016.
[37]	中华人民共和国卫生部. GB 19302-2010 食品安全国家标准发酵乳[S]. 北京: 中国标准出版社, 2010. Ministry of Health, PRC. GB 19302-2010 National standard for food safety fermented milk[S]. Beijing: Standards Press of China, 2010.
[38]	王伟佳, 高晓夏月, 刘爱国, 等. 不同热处理无乳糖酸奶与普通酸奶品质的比较[J]. 食品与发酵工业,2021,47(5):99−104. [WANG Weijia, GAO Xiaoxiayue, LIU Aiguo, et al. Quality comparison of lactose free yoghurt and ordinary yoghurt with different heat treatment[J]. Food and Fermentation Industries,2021,47(5):99−104.
[39]	蔡静静, 张亚川, 李谞, 等. 新疆伊犁地区乳制品中乳酸菌发酵和益生特性及其复合发酵方案优化[J]. 食品科学,2020,41(18):172−179. [CAI Jingjing, ZHANG Yachuan, LI Xu, et al. Fermentation and probiotic properties of lactic acid bacteria isolated from dairy products in yili, xinjiang and optimization of their mixtures for mixed-culture fermentation[J]. Food Science,2020,41(18):172−179. doi: 10.7506/spkx1002-6630-20190724-320
[40]	陈南, 高浩祥, 何强, 等. 共发酵技术对番茄酸奶品质的影响[J]. 广东化工,2017,44(22):9−11. [CHEN Nan, GAO Haoxiang, HE Qiang, et al. Effect of co-fermentation on the quality of tomato yogurt[J]. Guangdong Chemical Industry,2017,44(22):9−11. doi: 10.3969/j.issn.1007-1865.2017.22.005
[41]	王婷, 张国权. 苦荞花生复合发酵酸奶加工工艺研究[J]. 食品工业,2013,34(9):17−21. [WANG Ting, ZHANG Guoquan. Research on processing technology of set tartary buckwheat-peanut yoghourt[J]. The Food Industry,2013,34(9):17−21.
[42]	孔凡平, 郎秀杰, 郭成宇. 凝固型双蛋白酸奶的发酵配方优化[J]. 食品工业,2021,42(1):47−52. [KONG Fanping, LANG Xiujie, GUO Chengyu. Optimization of fermentation formula of coagulated double protein yoghurt[J]. The Food Industry,2021,42(1):47−52.
[43]	赵岩岩, 赵圣明, 李帅, 等. 山楂叶多糖对发酵乳品质及抗氧化活性的影响[J]. 食品科学,2020,41(2):73−79. [ZHAO Yanyan, ZHAO Shengming, LI Shuai, et al. Effect of polysaccharides from hawthorn leaves on the quality and antioxidant activity of fermented milk[J]. Food Science,2020,41(2):73−79. doi: 10.7506/spkx1002-6630-20181213-159
[44]	中华人民共和国卫生部. GB 4789.35-2016食品安全国家标准食品微生物学检验乳酸菌检验[S]. 北京: 中国标准出版社, 2016. Ministry of Health, PRC. GB 4789.35-2016 National standard for food safety food microbiology test lactic acid bacteria test[S]. Beijing: Standards Press of China, 2016.
[45]	赵旭飞, 胡志和, 薛璐, 等. 超高压处理对脱脂乳感官特性的影响[J]. 食品科学,2019,40(19):172−184. [ZHAO Xufei, HU Zhihe, XUE Lu, et al. Effect of ultra-high pressure treatment on sensory quality of skim milk[J]. Food Science,2019,40(19):172−184. doi: 10.7506/spkx1002-6630-20190415-198
[46]	冯永强, 贾凌云, 胡志和, 等. 无乳糖酸乳的制备及与普通酸乳的比较研究[J]. 乳业科学与技术,2018,41(6):12−17. [FENG Yongqiang, JIA Lingyun, HU Zhihe, et al. Preparation of lactose-free yogurt and comparison with ordinary yogurt[J]. Journal of Dairy Science and Technology,2018,41(6):12−17.
[47]	贾凌云, 胡志和, 薛璐, 等. 不同温度发酵无乳糖酸奶与普通酸奶差异性比较[J]. 食品科学,2019,40(23):79−90. [JIA Lingyun, HU Zhihe, XUE Lu, et al. Comparison of lactose-free and plain yoghurt fermented at different temperatures[J]. Food Science,2019,40(23):79−90. doi: 10.7506/spkx1002-6630-20190515-167
[48]	中国乳制品工业协会. RHB 103-2004酸牛乳感官质量评鉴细则[S]. 北京: 中国标准出版社, 2004. China Dairy Industry Association. RHB 103-2004 Rules for sensory quality evaluation of sour milk[S]. Beijing: Standards Press of China, 2004.
[49]	王文超. 微生物谷氨酰胺转氨酶作用于酸奶蛋白凝胶特性及抗氧化机理研究[D]. 杭州: 浙江科技学院, 2017. WANG Wenchao. Study on gel properties and antioxidant mechanism of protein from yoghurt treated by mTG[D]. Hangzhou: Zhejiang University of Science and Technology, 2017.
[50]	李晨, 张国文, 赵云, 等. 酸奶后酸化中保加利亚乳杆菌关键基因表达分析[J]. 中国食品学报,2018,18(7):256−262. [LI Chen, ZHANG Guowen, ZHAO Yun, et al. Gene expression analysis of yogurt post-acidification in Lactobacillus delbrueckii subsp. bulgaricus[J]. Journal of Chinese Institute of Food Science and Technology,2018,18(7):256−262.
[51]	牟英杰, 林海君, 杨丽杰, 等. 谷氨酰胺转胺酶对搅拌型酸奶品质的影响[J]. 食品工业科技,2013,34(24):155−158, 172. [MU Yingjie, LIN Haijun, YANG Lijie, et al. Effect of transglutaminase on the quality of stirred yoghurt[J]. Science and Technology of Food Industry,2013,34(24):155−158, 172.
[52]	张艳. 巴氏杀菌酸羊奶生产技术研究[D]. 西安: 陕西师范大学, 2019. ZHANG Yan. Study on production technology of pasteurized yogurt milk[D]. Xi’an: Shaanxi Normal University, 2019.
[53]	付颖. 微生物谷氨酰胺转氨酶对酸奶品质的影响[D]. 上海: 华东师范大学, 2005. FU Ying. Effeets of MTG on yoghourt quality[D]. Shanghai: East China Normal University, 2005.
[54]	杨敏. 凝固型酸奶加工技术应用研究[D]. 武汉: 湖北工业大学, 2016. YANG Min. Study on processing technology of solidified yoghurt[D]. Wuhan: Hubei University of Technology, 2016.
[55]	ARDELEAN A I, OTTO C, JAROS D, et al. Transglutaminase treatment to improve physical properties of acid gels from enriched goat milk[J]. Small Ruminant Research,2012,106(1):47−53. doi: 10.1016/j.smallrumres.2012.05.002
[56]	苏海龙. 转谷氨酰胺酶在凝固型酸奶中的应用研究[D]. 保定: 河北农业大学, 2003. SU Hailong. The study of transgluminase's application in firm yoghurt[D]. Baoding: Hebei Agricultural University, 2003.
[57]	李东红, 贾先勇, 杨晋杰, 等. 碱性蛋白酶和谷氨酰胺转氨酶对无麸质谷物馒头发酵过程中风味特性的影响[J]. 食品工业科技,2020,41(22):62−69. [LI Donghong, JIA Xianyong, YANG Jinjie, et al. Effects of alkaline protease and transglutaminase on flavor characteristics of gluten-free grain steamed buns during fermentation[J]. Science and Technology of Food Industry,2020,41(22):62−69.