Immunomodulatory Effect of Triple Probiotics and Fructo-Oligosaccharide Compound Powder in Mice

SU Shuonan; HOU Linzhong

doi:10.13386/j.issn1002-0306.2021090063

Volume 43 Issue 11

May 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(11): 363-368. > DOI: 10.13386/j.issn1002-0306.2021090063

SU Shuonan, HOU Linzhong. Immunomodulatory Effect of Triple Probiotics and Fructo-Oligosaccharide Compound Powder in Mice[J]. Science and Technology of Food Industry, 2022, 43(11): 363−368. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090063.

Citation:

PDF (2058 KB)

Immunomodulatory Effect of Triple Probiotics and Fructo-Oligosaccharide Compound Powder in Mice

SU Shuonan,
HOU Linzhong^,

Tangshan Workers Hospital, Tangshan 063003, China

More Information

Received Date: September 05, 2021
Available Online: March 24, 2022

Graphical Abstract

Abstract

Abstract

Objective: The present study aimed to evaluate immunomodulatory effects of triple probiotics (Bifidobacterium lactis V9/Lactobacillus plantarum P-8/Lactobacillus casei Zhang) and fructo-oligosaccharide (BLLF) in mice and its mechanism. Methods: C57BL/6J mice received intragastric administration of distilled water (control group 0.1 mL/10 g) and different doses of BLLF (triple probiotics 0.01 mg/kg+fructo-oligosaccharide 0.1 g/kg, triple probiotics 0.02 mg/kg+fructo-oligosaccharide 0.1 g/kg, and triple probiotics 0.06 mg/kg+fructo-oligosaccharide 0.1 g/kg) 0.1 mL/10 g once daily for 30 days. The immunity of mice was evaluated by organ ratios. The function of cellular immunity, monocyte macrophage, humoral immunity, and NK cell activities of the mice were further evaluated. Results: Compared with the control group, low, medium and high dose BLLF showed no significant effects on body weights and organ ratios of the mice(P>0.05). BLLF showed no significant effects on ConA-induced mouse splenic lymphocyte proliferations(P>0.05). Medium and high dose BLLF significantly increased the delayed hypersensitivity induced by dinitrofluorobenzene in mice (P < 0.05). The macrophage carbon clearance function of mice in high dose BLLF group was significantly increased compared with the control group (P < 0.05). Medium and high dose BLLF significantly increased the mouse peritoneal macrophage phagocytosis of chicken red blood cells（P < 0.05). Medium and high dose BLLF significantly increased hemolytic activity and number of antibody producing cells in mice (P < 0.05). Medium and high dose BLLF significantly increased NK cell activities in mice (P < 0.05). Conclusions: BLLF exhibited potent immunomodulatory effects by increasing cellular immunity, humoral immunity, function of monocyte macrophage, and NK cell activity.
- probiotics,
- Lactobacillus,
- fructo-oligosaccharide,
- mouse,
- immunomodulatory effect

FullText(HTML)

References (35)

References

[1]	柯程仁, 叶兰仙. 肠道菌群及益生菌对青少年抑郁症影响的研究进展[J]. 解放军医学杂志,2021,46(9):855−859. [KE C R, YE L X. Characteristics of intestinal flora and antidepressant effect of probiotics on adolescents[J]. Medical Journal of Chinese People's Liberation Army,2021,46(9):855−859. doi: 10.11855/j.issn.0577-7402.2021.09.02
[2]	YOUSEFI B, ESLAMID M, GHASEMIAN A, et al. Probiotics importance and their immunomodulatory properties[J]. J Cell Physiol,2019,234(6):8008−8018. doi: 10.1002/jcp.27559
[3]	PIQUE N, BERLANGA M, MINANA-GALBIS D. Health benefits of heat-killed (Tyndallized) probiotics: An overview[J]. Int J Mol Sci,2019,20(10):2534. doi: 10.3390/ijms20102534
[4]	陈莹, 江剑平. 益生菌在保健食品中的应用研究进展[J]. 保健医学研究与实践,2020,17(6):89−92. [CHEN Y, JIANG J P. Progress in applied research of probioticsin health care food[J]. Health Med Res and Prac,2020,17(6):89−92. doi: 10.11986/j.issn.1673-873X.2020.06.020
[5]	王淑梅, 邸维, 妥彦峰, 等. 益生菌的免疫调控作用研究进展[J]. 粮食与油脂,2021,34(5):23−26. [WANG S M, DI W, TUO F, et al. Research progress on immune regulation of probiotics[J]. Cereals & Oils,2021,34(5):23−26. doi: 10.3969/j.issn.1008-9578.2021.05.008
[6]	BRUCH-BERTANI J P, URIBE-CRUZ C, PASQUALOTTO A, et al. Hepatoprotective effect of probiotic Lactobacillus rhamnosus GG through the modulation of gut permeability and inflammasomes in a model of alcoholic liver disease in zebrafish[J]. J Am Coll Nutr,2020,39(2):163−170. doi: 10.1080/07315724.2019.1627955
[7]	XU H, HIRAISHI K, KURAHARA LH, et al. Inhibitory effects of breast milk-derived Lactobacillus rhamnosus Probio-M9 on colitis associated carcinogenesis by restoration of the gut microbiota in a mouse model[J]. Nutrients,2021,13(4):1143. doi: 10.3390/nu13041143
[8]	CUNA A, YU W, MENDEN H L, et al. NEC-like intestinal injury is ameliorated by Lactobacillus rhamnosus GG in parallel with SIGIRR and A20 induction in neonatal mice[J]. Pediatr Res,2020,88(4):546−555. doi: 10.1038/s41390-020-0797-6
[9]	YAN Y, LIU C, ZHAO S, et al. Probiotic Bifidobacterium lactis V9 attenuates hepatic steatosis and inflammation in rats with non-alcoholic fatty liver disease[J]. AMB Express,2020,10(1):101. doi: 10.1186/s13568-020-01038-y
[10]	SONG Y, HE Q, ZHANG J, et al. Genomic variations in probiotic Lactobacillus plantarum P-8 in the human and rat gut[J]. Front Microbiol,2018,5(9):893.
[11]	托娅, 张和平. 一株分离自内蒙古传统酸马奶中的乳酸杆菌Lactobacillus casei. Zhang对小鼠免疫功能的影响[J]. 中外医疗,2008,11:39−42. [TUO Y, ZHANG H P. Effect of Lactobacillus casei. Zhang isolated from traditional milk in Neimenggu on immunologic function of mice[J]. China Foreign Medical Treatment,2008,11:39−42. doi: 10.3969/j.issn.1674-0742.2008.17.026
[12]	白梅, 黄天, 郭帅, 等. 益生菌干酪乳杆菌Zhang和乳双歧杆菌V9发酵乳胞外多糖含量对流变学特性、质构和稳定性的影响[J]. 中国食品学报,2021,21(4):193−202. [BAI M, HUANG T, GUO S, et al. Effects of extracellular polysaccharide in yogurt produced by the probiotic bacteria, Lactobacillus casei Zhang and Bifidobacterium animalis subsp. lactis V9 on rheological propertiestexture and stability[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(4):193−202.
[13]	DAN T, CHEN H, LI T, et al. Influence of Lactobacillus plantarum P-8 on fermented milk flavor and storage stability[J]. Front Microbiol,2019,9:3133. doi: 10.3389/fmicb.2018.03133
[14]	蒙月月, SATHI C, SHUVAN K S, 等. 植物乳杆菌KLDS 1.0318对小鼠免疫调节作用初步研究[J]. 食品工业科技,2018,39(7):303−308. [MENG Y Y, SATHI C, SHUVAN K S, et al. Preliminary study on the immunomodulatory function of Lactobacillus plantarum KLDS 1.0318 in mice[J]. Science and Technology of Food Industry,2018,39(7):303−308.
[15]	CARLA P, GEORGIOS S, ALESSIO T, et al. Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis[J]. Pharmacological Research,2021:105795.
[16]	FM A, PYDA A, AWB B, et al. A four-strain probiotic exerts positive immunomodulatory effects by enhancing colonic butyrate production in vitro[J]. International Journal of Pharmaceutics,2019,555:1−10. doi: 10.1016/j.ijpharm.2018.11.020
[17]	HS A, YK B, ED B. Bifidogenic effect and in vitro immunomodulatory roles of melibiose-derived oligosaccharides produced by the acceptor reaction of glucansucrase E81[J]. Process Biochemistry,2020,91:126−131. doi: 10.1016/j.procbio.2019.12.003
[18]	张金, 王美华, 杨永龙, 等. 低聚果糖和低聚半乳糖的研究进展及在乳制品中的应用[J]. 中国食品添加剂,2020,31(10):129−134. [ZHANG J, WANG M H, YANG Y L, et al. Research progress and application in dairy products of fructo-oligosaccharide and galacto-oligosaccharide[J]. China Food Additives,2020,31(10):129−134.
[19]	张慧, 贺延苓, 李琼, 等. 低聚果糖的生物药学效用及应用研究进展[J]. 药物生物技术,2019,26(5):446−448. [ZHANG H, HE Y L, LI Q, et al. Research advances of function effects and application of fructo-oligosaccharide[J]. Pharmaceutical Biotechnology,2019,26(5):446−448.
[20]	朱自平, 汪振炯, 朱华. 嗜酸乳杆菌复合益生菌片对小鼠免疫功能的影响及机制研究[J]. 食品研究与开发,2021,42(22):44−50. [ZHU Z P, WANG Z J, ZHU H. The effect and mechanism of Lactobacillus acidophilus probiotic compound tablets on immune function in mice[J]. Food Research and Development,2021,42(22):44−50.
[21]	刘冬英, 刘臻, 张丽婧, 等. 免疫防御蛋白粉对小鼠免疫功能的影响[J]. 食品安全质量检测学报,2021,12(5):1945−1949. [LIU D Y, LIU Z, ZHANG L J, et al. Effect of immune defence protein powder on immune function in mice[J]. Journal of Food Safety and Quality,2021,12(5):1945−1949.
[22]	吴琼, 张丽艳, 黄颖, 等. 参芪茯苓片拆方对小鼠免疫功能的影响[J]. 贵州中医药大学学报,2021,43(1):29−33. [WU Q, ZHANG L Y, HUANG Y, et al. Effect of Shenqifuling tablet on immune function in mice[J]. Journal of Guizhou University of Traditional Chinese Medicine,2021,43(1):29−33.
[23]	沙玥彤, 蔡胡强, 綦振亮, 等. 常青素对小鼠免疫功能的影响及机制研究[J]. 哈尔滨医科大学学报,2020,54(6):569−574. [SHA Y T, CAI H Q, QI Z L, et al. Effects of Changqingsu on immune function in mice and its mechanism[J]. Journal of Harbin Medical University,2020,54(6):569−574. doi: 10.3969/j.issn.1000-1905.2020.06.001
[24]	顾晓龙, 罗明友. 黄精对小鼠免疫调节作用的影响研究[J]. 四川畜牧兽医,2019,46(4):24−26. [GU X L, LUO M Y. Study on the Immunomodulation effect of Polygonatum sibircum on mice[J]. Sichuan Animal & Veterinary Sciences,2019,46(4):24−26.
[25]	檀新珠, 吴晓晴, 吕明其, 等. 太子参茎叶多糖对小鼠免疫器官指数和血清免疫球蛋白、补体含量的影响[J]. 福建农林大学学报(自然科学版),2017,46(5):590−594. [TAN X Z, WU X Q, LV M Q, et al. Effect of Radix pseudostellariae stem and leaf polysaccharides on immune organ index and immunoglobulin complement in serum of mice[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2017,46(5):590−594.
[26]	FU H, LIN X, FU H, et al. Effects of compound Phellinus igniarius(L. ex Fr.) Quel. oral liquids polysaccharide on the immunologic function of mice[J]. Agricultural Science & Technology,2015,16(6):1290−1294.
[27]	李凤文, 覃辉艳, 赵鹏, 等. 黄芪胶囊增强免疫力的实验研究[J]. 应用预防医学,2008,14(6):330−333. [LI F W, TAN H Y, ZHAO P, et al. Experimental research of Huangqi capsule on enhancement of immunologic function[J]. Applied Preventive Medicine,2008,14(6):330−333. doi: 10.3969/j.issn.1673-758X.2008.06.004
[28]	钟媛媛, 杨晓涵, 张要武, 等. 三七多糖对小鼠免疫功能的影响[J]. 华西药学杂志,2016,31(6):573−576. [ZHONG Y Y, YANG X H, ZHANG Y W, et al. Effect of Panax notoginseng polysaccharide on immunologic function of mice[J]. West China Journal of Pharmaceutical Sciences,2016,31(6):573−576.
[29]	郑纯威, 陈宇, 丁华熳, 等. 复方氨基酸口服液对小鼠免疫功能影响的实验研究[J]. 中国中医药科技,2010,17(2):108−109. [ZHENG C W, CHEN Y, DING H M, et al. Effect of amino acid complex on immunologic function of mice[J]. Chinese Journal of Traditional Medical Science and Technology,2010,17(2):108−109. doi: 10.3969/j.issn.1005-7072.2010.02.010
[30]	陈冬金, 黄志坚, 陈强. 复方黄芩颗粒剂对小鼠免疫功能的影响[J]. 江西农业大学学报,2007,5:809−812. [CHEN D J, HUANG Z J, CHEN Q. Effect of compound Radix scutellariae granule on immunologic function of mice[J]. Acta Agriculturae Universitatis Jiangxiensis,2007,5:809−812. doi: 10.3969/j.issn.1000-2286.2007.05.025
[31]	朱艳, 姜盛, 李明亮, 等. 紫苏籽蛋白对免疫力低下小鼠免疫调节功能的研究[J]. 食品工业科技,2020,41(21):322−326, 332. [ZHU Y, JIANG S, LI M L, et al. Study on immune regulation of Perilla frutescens seed protein in mice with low immunity[J]. Science and Technology of Food Industry,2020,41(21):322−326, 332.
[32]	赵雯, 刘伟贤, 巴根纳, 等. 热灭活乳双歧杆菌 BL-99 缓解小鼠结肠炎及调节肠道菌群的功能研究[J]. 中国乳品工业,2020,48(11):4−10. [ZHAO Wen, LIU Weixian, BA Genna, et al. Function research of heat-inactivated Bifidobacterium lactis BL-99 in ameliorating colitis and regulating intestinal microbiota in mice[J]. China Dairy Industry,2020,48(11):4−10.
[33]	翟云, 王雅菲, 托娅. 乳双歧杆菌 M8 对免疫抑制模型大鼠免疫功能的影响[J]. 吉林大学学报(医学版),2020,46(3):582−588. [ZHAI Y, WANG Y F, TUO Y. Effect of Bifidobacterium lactis M8 on immune function of immunosuppressive model rats[J]. Journal of Jilin University (Medicine Edition),2020,46(3):582−588.
[34]	FINAMORE A, ROSELLI M, DONINI L, et al. Supplementation with Bifidobacterium longum Bar33 and Lactobacillus helveticus Bar13 mixture improves immunity in elderly humans (over 75 years) and aged mice[J]. Nutrition,2019,63(64):184−192.
[35]	DE W R, CLAASSEN E, BOKKEN G, et al. Enhanced immunological memory responses to listeria monocytogenes in rodents, as measured by delayed-type hypersensitivity (DTH), adoptive transfer of DTH, and protective immunity, following lactobacillus casei shirotaingestion[J]. Clinical and Vaccine Immunology,2003,10(1):59−65. doi: 10.1128/CDLI.10.1.59-65.2003

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	乔增辉，位璐璐，卢祺，史羽瑶，尹明雨，王锡昌. 保活方式对中华绒螯蟹活力和生理代谢的影响. 水产科学. 2025(01): 56-64 .
2.	张亮子，黄泽南，尤娟，刘茹，尹涛，马华威. 保活运输应激对水产动物肌肉品质影响的研究进展. 广东海洋大学学报. 2024(01): 35-43 .
3.	阮记明，钟智威，黄建珍，温娇萍，王朝，傅雪军，王润萍. 克氏原螯虾低温休眠保活技术研究. 中国水产科学. 2024(07): 810-819 .
4.	李彤彤，赵玲，王善宇，刘淇，曹荣. 太平洋牡蛎活体冷藏过程中脂质及挥发性物质变化. 肉类研究. 2024(10): 23-29 .

Other cited types(2)

Get Citation

PDF

XML

Article Metrics

Article views (247) PDF downloads (26) Cited by(6)

Science and Technology of Food Industry

Immunomodulatory Effect of Triple Probiotics and Fructo-Oligosaccharide Compound Powder in Mice

Abstract

References

Cited by

Periodical cited type(4)

Other cited types(2)

Catalog

Article Metrics

Export File

Citation

Format

Content