Effects of Extraction Methods on the Physicochemical Properties and Bioactivities <i>in Vitro</i> of Raspberry (<i>Rubus idaeus</i> L.) Polysaccharides

TIAN Yingpeng; CHEN Jie; WANG Lei; XU Fei

doi:10.13386/j.issn1002-0306.2021090060

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 1-10. > DOI: 10.13386/j.issn1002-0306.2021090060

TIAN Yingpeng, CHEN Jie, WANG Lei, et al. Effects of Extraction Methods on the Physicochemical Properties and Bioactivities in Vitro of Raspberry (Rubus idaeus L.) Polysaccharides[J]. Science and Technology of Food Industry, 2022, 43(8): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090060.

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Effects of Extraction Methods on the Physicochemical Properties and Bioactivities in Vitro of Raspberry (Rubus idaeus L.) Polysaccharides

College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China

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Received Date: September 05, 2021
Available Online: February 20, 2022

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Abstract

Abstract

To determine the effects of extraction on the physicochemical properties and bioactivities in vitro of raspberry polysaccharides (raspberry polysaccharides, RAPs), water extraction, alkali extraction, acid extraction and enzyme extraction methods were studied. The microstructure of the polysaccharides and function groups were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The rheological properties of the polysaccharide were determined by HR-1 Discovery mixed rheometer. Additionally, the antioxidant activities were evaluated by DPPH, ABTS⁺ and hydroxyl radical scavenging activities, and the hypoglycemic activities were analyzed by α-amylase and α-glucoside inhibition. Furthermore, their hypidemic activities were evaluated by their oil, cholesterol and sodium cholate binding activities. The results showed that extraction yields, total polysaccharides and their molecular weights, constituent monosaccharides, proteins and phenolic compounds, rheological properties and binding capacities in vitro of the RAPs varied depending on the extraction method employed. RAPs obtained by enzyme extraction exhibited the highest molecular weight (5.80×10⁵ Da) and apparent viscosity. Moreover, in terms of biological activity, they showed strong antioxidant activities and significant α-amylase and α-glucosidase inhibitory effects. And the raspberry polysaccharides obtained by enzyme extraction exhibited higher cholesterol binding capacity (22.92±0.41mg/g) and bile acid-binding capacity (83.55%±0.16%). In conclusion, compared with the other three methods, polysaccharides extracted by enzyme extraction showed stronger biological activity in vitro. The results from this study might provide a scientific basis for the isolation and application of RAPs in the pharmaceutical and functional food industries.
- : Rubus idaeus L.,
- polysaccharide,
- extraction methods,
- physicochemical properties,
- bioactivities in vitro

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References

[1]	YU Z, Liu L, XU Y, WANG L, et al. Characterization and biological activities of a novel polysaccharide isolated from raspberry (Rubus idaeus L.) fruits[J]. Carbohydr Polymers,2015,132:180−186. doi: 10.1016/j.carbpol.2015.06.068
[2]	GAO J, LIN L, SUN B, et al. A comparison study on polysaccharides extracted from Laminaria japonica using different methods: Structural characterization and bile acid-binding capacity[J]. Food Function,2017,8(9):3043−3052. doi: 10.1039/C7FO00218A
[3]	WANG L, ZHANG B, XIAO J, et al. Physicochemical, functional, and biological properties of water-soluble polysaccharides from Rosa roxburghii Tratt fruit[J]. Food Chemistry,2018,249(May30):127−135.
[4]	DINA R, SERGIO S, ALINE S, et al. Impact of enzyme and ultrasound-assisted extraction methods on biological properties of red, brown, and green seaweeds from the central west coast of Portugal[J]. Food Chemistry,2015,12:3177−3188.
[5]	李超. 药食同源夏枯草多糖的分离纯化、结构鉴定及生物活性研究[D]. 广州: 华南理工大学, 2015 LI C. Isolation, purification and structural identification of polysaccharides from Prunella vulgaris Linn and their biological activities[D]. Guangzhou: South China University of Technology, 2015.
[6]	NIE X R, LI H Y, DU G, et al. Structural characteristics, rheological properties, and biological activities of polysaccharides from different cultivars of okra (Abelmoschus esculentus) collected in China[J]. International Journal of Biological Macromolecules,2019,139:459−467. doi: 10.1016/j.ijbiomac.2019.08.016
[7]	汪磊. 刺梨多糖的分离纯化、降血糖作用及其对肠道微生态的影响[D]. 广州: 华南理工大学, 2019 WANG L. Isolation, purification and hypoglycemic activity of polysaccharides from Rosa roxburghii Tratt fruit and their effect on gut microflora[D]. Guangzhou: South China University of Technology, 2019.
[8]	DOU M D, CHEN C, HUANG Q, et al. Comparative study on the effect of extraction solvent on the physicochemical properties and bioactivity of blackberry fruit polysaccharides[J]. International Journal of Biological Macromolecules, 2021, 183: 1548-1599.
[9]	QIN Y, LI X, LI M, et al. Preparation of bioactive polysaccharide nanoparticles with enhanced radical scavenging activity and antimicrobial activity[J]. Journal of Agricultural Food Chemistry,2018,66:4373−4383. doi: 10.1021/acs.jafc.8b00388
[10]	RE R, PELLEGRINI N. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology Medicine,1999,26:1231−1237. doi: 10.1016/S0891-5849(98)00315-3
[11]	MENG Y, SU A, YUAN S, et al. Evaluation of total flavonoids, myricetin, and quercetin from Hovenia dulcis Thunb. as inhibitors of α-amylase and α-glucosidase[J]. Plant Foods for Human Nutrition,2016,71(4):1−6.
[12]	TONG T, LI J, KO D O, et al. In vitro antioxidant potential and inhibitory effect of seaweed on enzymes relevant for hyperglycemia[J]. Food Science Biotechnology,2014,23(6):2037−2044. doi: 10.1007/s10068-014-0277-z
[13]	LUO X L, QI W, DONG F, et al. Modification of insoluble dietary fibers from bamboo shoot shell: Structural characterization and functional properties[J]. International Journal of Biological Macromolecules, 2018, 120: 1461-1467.
[14]	曾红亮. 金桔多糖结构表征及降血脂机理的研究[D]. 福州: 福建农林大学, 2015. ZENG H L. Structure characterization and hypolipidemic mechanism of polysaccharides from Fortunella margarita (Lour.) Swingle[D]. Fuzhou: Fujian Agriculture and Forest University, 2015
[15]	游丽君, 张云林, 温玲蓉, 等. 不同方法对龙须菜多糖性质的影响[J]. 现代食品科技, 2016, 32(6): 148-155, 182 YOU L J, ZHANG Y L, WEN L, et al. Effect of extraction method on the properties of polysaccharides from Gracilaria lemaneiformis[J]. Modern Food Science and Technology 2016, 32(6): 148-155, 182.
[16]	YAN J K, DING Z C, GAO X L, et al. Comparative study of physicochemical properties and bioactivity of Hericium erinaceus polysaccharides at different solvent extractions[J]. Carbohydrate Polymers,2018,193:73−382. doi: 10.1016/j.carbpol.2018.03.086
[17]	赵晨淏, 刘钧发, 冯梦莹, 等. 不同提取方法对龙眼多糖性质的影响[J]. 现代食品科技, 2012, 28(10): 1298-1302. ZHAO C H, LIU J F, FENG M Y, et al. Effect of different extraction methods on the properties of longan polysaccharides[J]. Modern Food Science and Technology, 2012, 28(10): 1298-1302.
[18]	YUAN Q, HE Y, XIANG P Y, et al. Influences of different drying methods on the structural characteristics and multiple bioactivities of polysaccharides from okra (Abelmoschus esculentus)[J]. International Journal of Biological Macromolecules,2020,147:1053−1063. doi: 10.1016/j.ijbiomac.2019.10.073
[19]	LI Q S, WANG L, Li J, et al, Characterization and antioxidant activities of degraded polysaccharides from two marine Chrysophyta[J]. Food Chemistry, 2014, 160: 1-7.
[20]	PAN C, ZHU Y M, ZHU Z Y, et al. Chemical structure and effects of antioxidation and against α-glucosidase of natural polysaccharide from Glycyrrhiza inflata Batalin[J]. International Journal of Biological Macromolecules, 2020, 155: 560-571.
[21]	石德玲, 齐俊华, 卢海燕, 等. 海参硫酸多糖的高温高压降解工艺及其降解机制[J]. 中国海洋生物, 2019, 38(1): 1−10. SHI D L, QI J H, LU H Y, et al. Hydrothermal degradation process and mechanism of sulfated polysaccharides from sea cucmber[J]. Chinese Journal Marine Drugs, 2019, 38(1): 1−10.
[22]	郭元亨, 张利军, 曹丽丽, 等. 植物多糖中单糖组成分析技术的研究进展[J]. 食品科学,2018,39(1):326−332. [[GUO Y H, ZHANG L J, CAO L L, et al. Recent advances in analytical techniques for monosaccharide composition of plant polysaccharides[J]. Food Science,2018,39(1):326−332. doi: 10.7506/spkx1002-6630-201801049
[23]	苏平, 孙昕, 宋思圆, 等. 提取方法对黄秋葵花多糖的结构组成及抗氧化活性的影响[J]. 食品科学,2018,39(15):1002−6630. [SU P, SUN X, SONG S Y, et al. Effect of extraction method on structure and antioxidant activity of polysaccharides from okra flowers[J]. Food Science,2018,39(15):1002−6630.
[24]	LI W, LIU H M, QIN G Y, et al. Structure characterization and antioxidant activity of polysaccharides from Chinese quince seed meal[J]. Food Chemitry,2017,234:314−322. doi: 10.1016/j.foodchem.2017.05.002
[25]	WANG R, CHEN P, JIA F, et al. Optimization of polysaccharides from Panax japonicus C. A. Meyer by RSM and its anti-oxidant activity[J]. International Journal of Biological Macromolecules,2012,50(2):331−336. doi: 10.1016/j.ijbiomac.2011.12.023
[26]	QIN Y, LIN S, FU Y, et al. Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus)[J]. International Journal of Biological Macromolecules,2019,127:178−186. doi: 10.1016/j.ijbiomac.2019.01.042
[27]	聂琳然, 郝利民, 王滔滔, 等. 不同来源红缘拟层孔菌粗多糖的抗氧化活性[J]. 食品科学,2019,40(19):1002−6630. [NIE L R, HE L M, WANG T T, et al. Antioxidant activity of crude polysaccharides from Fomitopsis pinicola from different geographical origins[J]. Food Science,2019,40(19):1002−6630.
[28]	周洋, 杨得坡, 钱纯果, 等. 阳春砂根茎多糖分离纯化、结构表征及抗氧化活性[J]. 食品与发酵工业,2021,47(16):52−58. [ZHOU Y, YANG D P, QIAN D G, et al. Purification, structural characterization and antioxidant activity of polysaccharide from the rhizome of Amomum villosum Lour[J]. Food Science,2021,47(16):52−58.
[29]	LIU J, ZHAO Y P, WU Q X, et al. Structure characterisation of polysaccharides in vegetable "okra" and evaluation of hypoglycemic activity[J]. Food Chemistry,2018,242:211−216. doi: 10.1016/j.foodchem.2017.09.051
[30]	GONG L, ZHANG, H, NIU, Y, C, et al. A novel alkali extractable polysaccharide from Plantago asiatic L. seeds and its radical-scavenging and bile acid-binding activities[J]. Journal of Agricultural and Food Chemistry, 63: 569-577.
[31]	YAN J K, LI X W, QIAO Z R, et al. Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices[J]. Food Chemistry,2019,271:588−596. doi: 10.1016/j.foodchem.2018.08.012