Bioactive Composition, Antioxidant, and <i>α</i>-Glucosidase Inhibition of Edible <i>Areca catechu</i> Waste Seed

ZHANG Peiyue; TANG Minmin; SONG Fei; LIU Can; CHEN Hua

doi:10.13386/j.issn1002-0306.2021090023

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 253-260. > DOI: 10.13386/j.issn1002-0306.2021090023

ZHANG Peiyue, TANG Minmin, SONG Fei, et al. Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed[J]. Science and Technology of Food Industry, 2022, 43(12): 253−260. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090023.

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Bioactive Composition, Antioxidant, and α-Glucosidase Inhibition of Edible Areca catechu Waste Seed

ZHANG Peiyue^{1, 2,},
TANG Minmin^{2, 3},
SONG Fei^{2, 3},
LIU Can^{1, 2},
CHEN Hua^{1, 2, 3, ,}

1.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2.
Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China
3.
Hainan Engineering Research Center of Arecanut Industry, Wenchang 571339, China

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Received Date: September 02, 2021
Available Online: April 10, 2022

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Abstract

Abstract

In view of the problem that edible Areca catechu seeds treated as waste and cannot be massively used after processing, in this study, the waste seeds after edible Areca catechu processing (PAS) are used. 75% ethanol extract of PAS (processed seed extraction, PSE) was extracted assisted by ultrasonic with a 75% ethanol solution as the extraction solvent. The contents of total polyphenols, total flavonoids and proanthocyanidins in the PSE were determined. Three methods (radical scavenging activities and Fe³⁺ reduction) were used to evaluate the antioxidant activity of PSE. Then the inhibitory of PSE on the activity of α-glucosidase was studied. The result showed that PSE was rich in polyphenols and flavonoid. The total contents of polyphenols, flavonoids and proanthocyanidins were (127.29±5.16) milligram gallic acid, (421.84±13.82) milligram rutin, and (87.83±6.60) milligram epicatechin in per gram of PSE, respectively. PSE contains 44 flavonoids were detected in PSE, in which the relative content of catechins was up to 77.27%. The IC₅₀ for scavenging DPPH free radical and ABTS⁺ free radical of PSE were (310.10±0.62) and (150.10±11.57) μg/mL, respectively. In addition, PSE had significant inhibitory effect on α-glucosidase, and its ability to inhibit α-glucosidase (IC₅₀=(90.10±1.89) μg/mL) was significantly stronger (P<0.05) than acabosose as a positive control (IC₅₀=(453.60±4.02) μg/mL). Therefore, it is necessary to recycle the PAS.
- adible Areca catechu,
- waste seed,
- total polyphenols,
- total flavonoids,
- proanthocyanidins,
- antioxidant activity,
- α-glucosidase

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