Simultaneous Determination of Nine Components in Ligustri Lucidi Fructus from Different Habitats by UPLC-ECD

CHUN Zeli; ZHU Ya; CHEN Rongxiang

doi:10.13386/j.issn1002-0306.2021090005

Volume 43 Issue 8

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(8): 305-311. > DOI: 10.13386/j.issn1002-0306.2021090005

CHUN Zeli, ZHU Ya, CHEN Rongxiang. Simultaneous Determination of Nine Components in Ligustri Lucidi Fructus from Different Habitats by UPLC-ECD[J]. Science and Technology of Food Industry, 2022, 43(8): 305−311. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090005.

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Simultaneous Determination of Nine Components in Ligustri Lucidi Fructus from Different Habitats by UPLC-ECD

CHUN Zeli^{1, 2,},
ZHU Ya³,
CHEN Rongxiang^{1, 2, ,}

1.
Analytical and Testing Center, Zunyi Medical University, Zunyi 563000, China
2.
Zunyi Engineering Technology Research Center of Physical and Chemical Analysis, Zunyi 563000, China
3.
School of Pharmacy, Zunyi Medical University, Zunyi 563000, China

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Received Date: September 01, 2021
Available Online: February 13, 2022

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Abstract

Abstract

A method was devoloped for the determination of hydroxytyrosol, protocatechuic acid, tyrosol, salidroside, verbascoside, specnuezhenide, luteolin, oleuropein and rutin in Ligustri Lucidi Fructus from different habitats by ultra-high performance liquid chromatography-electrochemical detection (UPLC-ECD). Ligustri Lucidi Fructus powder (0.25 g) was extracted ultrasonically for 20 min with 10 mL of 80% methanol. The extract was filtered through microporous membrane of 0.22 μm and then separeted by a Waters ACQUITY UPLC BEH shield RP 18 (2.1 mm×100 mm, 1.7 μm) chromatographic column. Methanol−50 mmol·L⁻¹ potassium citrate solution with gradient elution was employed as the mobile phase. The flow rate was 0.4 mL·min⁻¹, the column temperature was maintained at 45 ℃ and the injection volume was 1 μL. The potential of electrochemical detector was set at 450 mV in the first channel and 700 mV in the second channel. The results showed that the linearity of nine constituents was good in the range of their mass concentration (R²≥0.9993). RSD of precision, repeatability, and stability (12 h) were less than or equal to 2.5%. The average recovery rates were from 97.9% to 102.2% and the RSD was in the range of 2.4%～4.6%. The method in this study was rapid, simple, sensitive, stable and reliable. It could be applied for simultaneous determination of nine components in Ligustri Lucidi Fructus, which would provide a reference for the quality control of Ligustri Lucidi Fructus.

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References

[1]	国家药典委员会. 中华人民共和国药典(2020版)[M]. 北京: 中国医药科技出版社, 2020: 47−48. Chinese Pharmacopoeia Commission. Pharmacopoeia of the people’s republic of China (2020 edition)[M]. Beijing: China Pharmaceutical Science and Technology Press, 2020: 47−48.
[2]	李昌勤, 姚辰, 朱荣遥, 等. 基于DPPH、ABTS和FRAP的中药女贞子抗氧化谱效关系研究[J]. 中国中药杂志,2016,4(9):1670−1677. [LI Changqin, YAO Chen, ZHU Rongyao, et al. Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays[J]. China Journal of Chinese Materia Medica,2016,4(9):1670−1677.
[3]	陈进芳, 翁育才. 三种不同炮制方法对女贞子中7种化学成分变化的影响[J/OL]. 中国现代中药, 2020. https://doi.org/10.13313/j.issn.1673-4890.20200618010. CHEN Jinfang, WENG Yucai. Effects of three processing methods on changes of seven chemical constituents in Ligustri Lucidi Fructus[J/OL]. Modern Chinese Medicine, 2020. https://doi.org/10.13313/j.issn.1673-4890.20200618010.
[4]	张丹捷, 赵盼, 栾茹乔, 等. 酒蒸对女贞子活性成分体内分布的影响[J]. 中药材,2021,44(7):1605−1610. [ZHANG Danjie, ZHAO Pan, LUAN Ruqiao, et al. Effect of wine steaming on the distribution in vivo of active compounds in Ligustri Lucidi Fructus[J]. Journal of Chinese Medicinal Materials,2021,44(7):1605−1610.
[5]	纪鑫, 刘晓谦, 高陆, 等. 女贞子中三萜类成分的定性定量研究[J]. 中国中药杂志,2021,46(5):1168−1178. [JI Xin, LIU Xiaoqian, GAO Lu, et al. Qualitative and quantitative analysis on triterpenoids in Ligustri Lucidi Fructus[J]. China Journal of Chinese Materia Medica,2021,46(5):1168−1178.
[6]	杜亚朋, 王美, 李璐遥, 等. 基于化合物稳定性探讨炮制对含环烯醚萜类成分中药药性及功效影响的研究进展[J]. 中草药,2021,52(16):5039−5051. [DU Yapeng, WANG Mei, LI Luyao, et al. Research progress on effect of processing on properties and efficacy of traditional Chinese medicine containing iridoid terpenoids based on stability of compounds[J]. Chinese Traditional and Herbal Drugs,2021,52(16):5039−5051. doi: 10.7501/j.issn.0253-2670.2021.16.029
[7]	LIU R H, KANG X, XU L P, et al. Effects of the combined extracts of Herba Epimedii and Fructus Ligustri Lucidi on bone mineral content and bone turnover in osteoporotic rats[J]. BMC Complementary and Alternative Medicine,2015(15):112.
[8]	刘静, 赵海霞, 袁丁, 等. 女贞子及其有效成分延缓衰老作用的研究进展[J]. 时珍国医国药,2016,27(12):2974−2977. [LIU Jing, ZHAO Haixia, YUAN Ding, et al. Research advances of Fructus Ligustri Lucidi and its effective components on delaying aging[J]. Lishizhen Medicine and Materia Medica Research,2016,27(12):2974−2977.
[9]	覃小燕, 韩晓玲, 张媛, 等. 基于网络药理学的女贞子抗骨质疏松作用机制研究[J]. 中国药学杂志,2021,56(5):368−378. [QIN Xiaoyan, HAN Xiaoling, ZHANG Yuan, et al. Mechanism of Ligustrum lucidum in treatment of osteoporosis based on network pharmacology[J]. Chinese Pharmaceutical Journal,2021,56(5):368−378.
[10]	LI L, CHEN B B, ZHU R Y, et al. Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice[J]. Aging,2019,11(21):9348−9368. doi: 10.18632/aging.102376
[11]	战兴晓, 刘希光, 刘祥龙, 等. 女贞子多糖相对分子质量与组成分析[J]. 中草药,2019,50(18):488−4292. [ZHAN Xingxiao, LIU Xiguang, LIU Xianglong, et al. Molecular weight and composition determination of polysaccharide from Ligustrum lucidum[J]. Chinese Traditional and Herbal Drugs,2019,50(18):488−4292.
[12]	刘婵婵, 杨薪正, 钱卫东, 等. 中药女贞子的化学成分及药理作用研究进展[J]. 海峡药学,2021,33(2):37−39. [LIU Chanchan, YANG Xinzheng, QIAN Weidong, et al. Advances in research on chemical constituents and pharmacological effects ofLigustrum lucidum[J]. Strait Pharmaceutical Journal,2021,33(2):37−39. doi: 10.3969/j.issn.1006-3765.2021.02.012
[13]	刘亭亭, 王萌. 女贞子化学成分与药理作用研究进展中国实验方剂学[J]. 中国实验方剂学,2014,20(14):228−234. [LIU Tingting, WANG Meng. Research progress of chemical composition and pharmacological effects of Fructus Figustri Lucidi[J]. Chinese Journal of Experimental Traditional Medical Formulae,2014,20(14):228−234.
[14]	JU H Y, CHEN S C, WU K J, et al. Antioxidant phenolic profifile from ethyl acetate fraction of Fructus Ligustri Lucidi with protection against hydrogen peroxide-induced oxidative damage in SH-SY5Y cells[J]. Food and Chemical Toxicology,2012(50):492−502.
[15]	CHEN B B, WANG L L, LI L, et al. Fructus Ligustri Lucidi in osteoporosis: a review of its pharmacology, phytochemistry, pharmacokinetics and safety[J]. Molecules,2017(22):1469.
[16]	廖晓燕, 洪圆, 姜雯文, 等. HPLC-荧光法测定酒女贞子中羟基酪醇、红景天苷和酪醇[J]. 中国现代应用药学,2019,36(24):3064−3067. [LIAO Xiaoyan, HONG Yuan, JIANG Wenwen, et al. Determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus by HPLC with fluorescence detection[J]. Chinese Journal of Modern Applied Pharmacy,2019,36(24):3064−3067.
[17]	刘颖新, 冯传平, 刘利利, 等. 一测多评法同时测定二至丸中8个成分[J]. 药物分析杂志,2021,4(2):210−218. [LIU Yingxin, FENG Chuanping, LIU Lili, et al. Simultaneous determination of 8 components in Erzhi pills by QAMS method[J]. Chinese Journal of Pharmaceutical Analysis,2021,4(2):210−218.
[18]	张柏生, 李斌, 李茜, 等. 不同产地酒女贞子红景天苷、特女贞苷含量测定及聚类分析[J]. 世界中医药,2021,16(3):429−429. [ZHANG Bosheng, LI Bin, LI Qian, et al. Determination of salidroside and nuezhenide in different producing areas and cluster analysis[J]. World Chinese Medicine,2021,16(3):429−429.
[19]	刘美, 张娟, 肖炯昌, 等. HPLC 测定女贞子中特女贞苷的含量及指纹图谱的建立[J]. 中国现代中药,2019,21(12):1647−1652. [LIU Mei, ZHANG Juan, XIAO Jiongchang, et al. Determination of content of specnuezhenide and establishment of fingerprint for Ligustri Lucidi Fructus by HPLC[J]. Modern Chinese Medicine,2019,21(12):1647−1652.
[20]	YAO W F, DAI J, ZHENG C Z, et al. Quality assessment of Ligustri Lucidi Fructus by the simultaneous determination of six compounds and chemometric analysis[J]. Journal of Separation Science,2015,38(11):1822−1827. doi: 10.1002/jssc.201500094
[21]	赵丽敏, 卢广英, 杨书娟, 等. 基于酚类成分特征图谱的女贞子质量表征研究[J]. 北京中医药大学学报,2016,39(6):480−488. [ZHAO Limin, LU Guangying, YANG Shujuan, et al. Quality representation and correlation analysis of Ligustrum lucidum Ait. based on characteristic spectrum of drug system[J]. Journal of Beijing University of Traditional Chinese Medicine,2016,39(6):480−488. doi: 10.3969/j.issn.1006-2157.2016.06.009
[22]	ZHANG D J, SUN L L, LI H F, et al. Pharmacokinetic comparison of nine bioactive components in rat plasma following oral administration of raw and wine-processed Ligustri Lucidi Fructus by ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry[J]. Journal of Separation Science,2020,43(21):3995−4005. doi: 10.1002/jssc.202000625
[23]	胡雁萍, 赵迪, 李焕茹, 等. UPLC-Q-Exactive Orbitrap高分辨质谱定量分析不同产地、炮制前后女贞子中9种成分的含量[J/OL]. 天然产物研究与开发, 2021. https://kns.cnki.net/kcms/detail/51.1335.Q.20210416.1706.002.html. HU Yanping, ZHAO Di, LI Huanru, et al. UPLC-Q Exactive-Orbitrap high-resolution mass spectrometry quantitative analysis of the content of 9 components in Ligustri Lucidi Fructus from different producing areas and before and after processing[J/OL]. Natural Product Research and Development, 2021. https://kns.cnki.net/kcms/detail/51.1335.Q.20210416.1706.002.html.
[24]	LI H, YAO W F, LIU G N, et al. Application of UHPLC-ESI-Q-TOF-MS to identify multiple constituents in processed products of the Herbal Medicine Ligustri Lucidi Fructus[J]. Molecules,2017(22):689.
[25]	LI Z Y, LI Q, LU J, et al. LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of suspensions of traditional Chinese medicine Erzhi Wan and Ligustri Lucidi Fructus[J]. Journal of Pharmaceutical Analysis,2011,1(1):8−12. doi: 10.1016/S2095-1779(11)70002-8
[26]	周逸芝, 邹立思, 刘毅, 等. 胶束毛细管电泳测定女贞子饮片中6种指标成分的含量[J]. 中国药学杂志,2014,49(11):994−998. [ZHOU Yizhi, ZOU Lisi, LIU Yi, et al. Simultaneous determination of six index components in Ligustri Lucidi Fructus by MEKC-DAD[J]. Chinese Pharmaceutical Journal,2014,49(11):994−998.
[27]	程军, 刘延. 高效液相色谱-电化学检测法在药物分析中的应用[J]. 中国药学杂志,2017,52(20):1753−1771. [CHENG Jun, LIU Yan. Electrochemical Detection in high performance liquid chromatography for analysis of pharmaceutical drugs[J]. Chinese Pharmaceutical Journal,2017,52(20):1753−1771.
[28]	ZHU Z Y, ZHANG Y, WANG J H, et al. Sugaring-out assisted liquid-liquid extraction coupled with high performance liquid chromatography-electrochemical detection for the determination of 17 phenolic compounds in honey[J]. Journal of Chromatography A,2019,1601:104−114. doi: 10.1016/j.chroma.2019.06.023
[29]	罗敏, 顾雯, 何婷, 等. 超高效液相色谱-电化学检测法测定女贞子中的3种成分[J]. 中国药学杂志,2020,55(24):2006−2011. [LUO Min, GU Wen, HE Ting, et al. Determination of three constituent in Ligustri Lucidi Fructus by ultra-high performance liquid chromatography-electrochemical detection[J]. Chinese Pharmaceutical Journal,2020,55(24):2006−2011.
[30]	SCHIMPF K J, MEEK C C, LEFF R D, et al. Quantification of myo-inositol, 1, 5-anhydro- D-sorbitol, and D-chiro-inositol using high-performance liquid chromatography with electrochemical detection in very small volume clinical samples[J]. Biomedical Chromatography,2015,29(11):1629−1636. doi: 10.1002/bmc.3470