Analysis of Ultrasonic-Microwave Synergistic Enzyme Extraction of Soluble Dietary Fiber from Millet Bran and Its Antioxidant Activity

YAN Feixiang; ZHU Libin; ZHU Dan; MIAO Xinyue; NIU Guangcai; WEI Wenyi

doi:10.13386/j.issn1002-0306.2021080297

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 163-172. > DOI: 10.13386/j.issn1002-0306.2021080297

YAN Feixiang, ZHU Libin, ZHU Dan, et al. Analysis of Ultrasonic-Microwave Synergistic Enzyme Extraction of Soluble Dietary Fiber from Millet Bran and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(12): 163−172. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080297.

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Analysis of Ultrasonic-Microwave Synergistic Enzyme Extraction of Soluble Dietary Fiber from Millet Bran and Its Antioxidant Activity

YAN Feixiang^{1, 2,},
ZHU Libin^{1, 2},
ZHU Dan^3, ,,
MIAO Xinyue^{1, 2},
NIU Guangcai^{1, 2, ,},
WEI Wenyi^{1, 2}

1.
Food College, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2.
Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing 163319, China
3.
College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China

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Received Date: August 25, 2021
Available Online: April 11, 2022

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Abstract

Ultrasonic-microwave synergistic enzyme method was used to study the effects of liquid-material ratio, synergistic time, extraction temperature and compound enzyme addition on the yield of soluble dietary fiber (SDF) from millet bran. Response surface methodology was used to optimize the process, and the antioxidant activity of soluble dietary fiber from millet bran were analyzed. The results showed that the optimum extraction conditions of SDF from millet bran were as follows: The liquid-material ratio was 31:1 mL/g, the synergistic time was 21 min, the extraction temperature was 56 ℃, and the compound enzyme addition was 1.4%. Under these conditions, the yield of the soluble dietary fiber was 6.35% and its purity was 91.27%. The antioxidant activity showed that when the sample concentration was 14 mg/mL, the SDF reducing power of millet bran was 1.219, and its IC₅₀ values for DPPH radical scavenging rate, ABTS⁺ radical scavenging rate and hydroxyl radical scavenging rate were 2.45, 26.15 and 5.98 mg/mL respectively. The results indicated that the SDF from millet bran had good antioxidant activity.
- millet bran,
- soluble dietary fiber,
- ultrasonic-microwave synergistic enzyme extraction,
- process optimization,
- antioxidant activity

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