Protective Effect of EGCG on Hydrogen Peroxide-induced Apoptosis of Human Cardiomyocyte via Regulating miR-16-5p/AOC1 Axis

BI Yingxin; LIU Xianjun; MENG Xianglong; LIU Yue; WANG Mengyuan; ZHANG Yan; LI Zhandong; YIN Yuhe; LI Hao

doi:10.13386/j.issn1002-0306.2021080256

Volume 43 Issue 7

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(7): 376-383. > DOI: 10.13386/j.issn1002-0306.2021080256

BI Yingxin, LIU Xianjun, MENG Xianglong, et al. Protective Effect of EGCG on Hydrogen Peroxide-induced Apoptosis of Human Cardiomyocyte via Regulating miR-16-5p/AOC1 Axis[J]. Science and Technology of Food Industry, 2022, 43(7): 376−383. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080256.

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Protective Effect of EGCG on Hydrogen Peroxide-induced Apoptosis of Human Cardiomyocyte via Regulating miR-16-5p/AOC1 Axis

1.
School of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
2.
College of Food Engineering, Jilin Engineering Normal University, Changchun 130052, China
3.
Department of Burns Surgery, The First Hospital of Jilin University, Changchun 130021, China

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Received Date: August 24, 2021
Available Online: February 10, 2022

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Abstract

Abstract

Objective: To explore the molecular mechanism driving the tea polyphenol epigallocatechin-3-gallate (EGCG)-exerted protective effect on hydrogen peroxide (H₂O₂)-induced cell apoptosis of human cardiomyocyte HCM cell lines. Methods: HCM cells were stimulated with H₂O₂ to establish a vitro apoptosis model; the caspase-3 assay was used to assess the cell apoptosis of HCM cells; the CCK-8 assay was used to perform the cytotoxicity experiment; qPCR was used to detect the levels of AOC1 mRNA and miR-16-5p; Western blot was used to detect the protein level of AOC1; cells transfection was conducted to regulate the expression levels of AOC1 and miR-16-5p in HCM cells; TargetScan was used to predict the binding relationship and site between AOC1 mRNA 3’UTR and miR-16-5p, which was verified by the double luciferase reporter gene assay. Results: EGCG could significantly inhibit H₂O₂-induced apoptosis of HCM cells (P<0.05). In apoptotic cells, the expression of AOC1 was significantly decreased, and EGCG could significantly up-regulate the expression of AOC1, while knocking down AOC1 could significantly reverse the inhibition of EGCG on apoptosis (P<0.05). Meanwhile, the expression of miR-16-5p was significantly increased in apoptotic cells, and EGCG could significantly inhibit the expression of miR-16-5p, while up-regulation of miR-16-5p significantly reversed the inhibition of EGCG on apoptosis (P<0.05). Upregulation of miR-16-5p significantly reversed the promotion of AOC1 expression by EGCG in apoptotic cells. Finally, miR-16-5p can target inhibit the expression of AOC1, while over expression of AOC1 significantly reversed miR-16-5p-induced apoptosis of HCM cells. Conclusion: EGCG protected H₂O₂-induced apoptosis of HCM cells by regulating the miR-16-5p/AOC1 axis, which proposes AOC1 as a potential novel therapeutic target pro-tection against cardiomyocyte injury.
- epigallocatechin-3-gallate,
- human cardiomyocyte,
- miR-16-5p,
- AOC1,
- cell apoptosis,
- H₂O₂

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