Optimization of Fermentation and <i>in Vitro</i> Digestion of Fermented Mixed-Bean Milk by Response Surface Methodology

LÜ Mingshou; GAO Yixin; SHI Yanguo; LIU Linlin; SUN Bingyu; ZHU Xiuqing

doi:10.13386/j.issn1002-0306.2021080234

Volume 43 Issue 10

May 2022

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Science and Technology of Food Industry > 2022 > 43(10): 238-245. > DOI: 10.13386/j.issn1002-0306.2021080234

LÜ Mingshou, GAO Yixin, SHI Yanguo, et al. Optimization of Fermentation and in Vitro Digestion of Fermented Mixed-Bean Milk by Response Surface Methodology[J]. Science and Technology of Food Industry, 2022, 43(10): 238−245. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080234.

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Optimization of Fermentation and in Vitro Digestion of Fermented Mixed-Bean Milk by Response Surface Methodology

College of Food Engineering of Harbin University of Commerce, Key Laboratory of Food Science and Engineering of Heilongjiang Province/Key Laboratory of Grain Food and Comprehensive Processing of Grain Resource of Heilongjiang Province, Harbin 150028, China

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Received Date: August 22, 2021
Available Online: March 18, 2022

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Abstract

In this study, red beans, black beans and soybeans were utilized to produce fermented mixed-bean milk. The changes of total phenols, total flavonoid and antioxidant capacity of the product were investigated by single factor experiment and response surface methodology. The results showed that the optimum fermentation process of fermented mixed-bean milk was sucrose addition of 7.2%, transglutaminase addition of 0.63 g/L and fermentation for 10 h accompanying the sensory score of 88.1 points. After digested with gastric juice and intestinal juice, the total phenols content in fermented mixed-bean milk were increased by 25% and 61%, while total flavonoid were increased by 51% and 22%. Before digestion, DPPH, ABTS, hydroxyl radical clearance and reducing power were 47.71%, 20.53%, 41.45% and 0.22 respectively. After gastrointestinal juice digestion, the antioxidant indexes were increased by 35.0%, 48.1%, 43.1% and 27.3% respectively. After intestinal juice digestion, the reducing power of fermented mixed-bean milk was 10% lower than that of gastric juice digestion, but still higher than that of undigested sample. The total phenols content after digestion was positively correlated with DPPH radical scavenging rate, ABTS and hydroxyl radical scavenging rate (P < 0.05) and there was a positive correlation between total flavonoid and reducing power (P < 0.05). Consequently, the results could provide a reference for the future research and development of fermented mixed-bean milk.

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[1]	BATTISTINI C, GULLÓN B, ICHIMURA E S, et al. Development and characterization of an innovative synbiotic fermented beverage based on vegetable soybean[J]. Brazilian Journal of Microbiology,2018,49:303−309. doi: 10.1016/j.bjm.2017.08.006
[2]	刘静, 李湘利, 朱九滨, 等. 芡实黄豆复合发酵乳酸饮料的工艺优化及抗氧化性研究[J]. 食品科技,2017,42(12):116−120. [LIU J, LI X L, ZHU J B, et al. Process optimization and antioxidant activity of lactic acid beverage fermented by euryale ferox and soybean[J]. Food Science and Technology,2017,42(12):116−120. LIU J, LI X L, ZHU J B, et al. Process optimization and antioxidant activity of lactic acid beverage fermented by Euryale ferox and Soybean [J]. Food Science and Technology, 2017, 42 (12): 116-120.
[3]	SARKER A, CHAKRABORTY S, ROY M. Dark red kidney bean (Phaseolus vulgaris L.) protein hydrolysates inhibit the growth of oxidizing substances in plain yogurt[J]. Journal of Agriculture and Food Research,2020:100062.
[4]	LEE J H, HWANG C E, CHO E J, et al. Improvement of nutritional components and in vitro antioxidative properties of soy-powder yogurts using Lactobacillus plantarum[J]. Journal of Food and Drug Analysis,2018,26(3):1054−1065. doi: 10.1016/j.jfda.2017.12.003
[5]	李彤, 彭珍, 熊涛. 乳酸菌发酵对复合豆乳饮料营养成分、香气成分及抗氧化活性的影响[J]. 食品与发酵工业,2018,44(4):111−118. [LI T, PENG Z, XIONG T. Effects of lactic acid bacteria fermentation on nutritional components, aroma componets and antioxidant activity of compound soybean milk beverage[J]. Food and Fermentation Industry,2018,44(4):111−118. LI T, PENG Z, XIONG T. Effects of lactic acid bacteria fermentation on nutritional components, aroma componets and antioxidant activity of compound soybean milk beverage [J]. Food and Fermentation Industry, 2018, 44 (4): 111-118.
[6]	FERNANDES S, LIMA F S, RODRIGUES D, et al. Evaluation of the isoflavone and total phenolic contents of kefir fermented soymilk storage and after the in vitro digestive system simulation[J]. Food Chemistry,2017,229:373−380. doi: 10.1016/j.foodchem.2017.02.095
[7]	LEE J H, KIM B, HWANG C E, et al. Changes in conjugated linoleic acid and isoflavone contents from fermented soymilks using Lactobacillus plantarum P1201 and screening for their digestive enzyme inhibition and antioxidant properties[J]. Journal of Functional Foods,2018,43:17−28. doi: 10.1016/j.jff.2018.01.022
[8]	MORELL P, FISZMAN S, LLORCA E, et al. Designing added-protein yogurts: Relationship between in vitro digestion behavior and structure[J]. Food Hydrocolloids,2017,72:27−34. doi: 10.1016/j.foodhyd.2017.05.026
[9]	中华人民共和国国家卫生和计划生育委员会. GB 5009.239-2016食品安全国家标准食品酸度的测定[S]. 北京: 中国标准出版社, 2016. State Health and Family Planning Commission of the people’s Republic of China. GB 5009.239-2016 National food safety standard Determination of acidity of food[S]. Beijing: China Standards Press, 2016.
[10]	孙世鑫, 李科, 骆鹏飞, 等. 双菌种混合发酵制备富含γ-氨基丁酸酸奶工艺优化[J]. 食品工业科技,2021,42(16):129−137. [SUN S X, LI K, LUO P F, et al. Preparation of enriched by mixed fermentation of two strains γ-process optimization of aminobutyric acid yoghurt[J]. Science and Technology of Food Industry,2021,42(16):129−137. SUN S X, LI K, LUO P F, et al. Preparation of enriched by mixed fermentation of two strains γ-Process optimization of aminobutyric acid yoghurt [J]. Food Industry Science and Technology, 2021, 42 (16): 129-137.
[11]	陈怡, 刘洋, 蒋立文, 等. 浏阳豆豉发酵过程中抗氧化成分及活性变化研究[J]. 食品工业科技,2020,41(19):273−278. [CHEN Y, LIU Y, JIANG L W, et al. Study on changes of antioxidant components and activity during Liuyang Douchi fermentation[J]. Science and Technology of Food Industry,2020,41(19):273−278. CHEN Y, LIU Y, JIANG L W, et al. Study on changes of antioxidant components and activity during Liuyang Douchi fermentation [J]. Food Industry Science and Technology, 2020, 41 (19): 273-278.
[12]	LEE M K, KIM J K, LEE S Y. Effects of fermentation on SDS-PAGE patterns, total peptide, isoflavone contents and antioxidant activity of freeze-thawed tofu fermented with Bacillus subtilis[J]. Food Chemistry,2018,249:60−65. doi: 10.1016/j.foodchem.2017.12.045
[13]	XU Y, ZHAO H, YAN X, et al. Preparation of a probiotic rice tablet: Sensory evaluation and antioxidant activity during gastrointestinal digestion[J]. LWT-Food Science and Technology,2019,124(1):108911.
[14]	任婷婷, 岳田利, 魏欣, 等. 益生菌发酵苹果浆工艺优化及发酵前后挥发性风味成分分析[J]. 食品科学,2019,40(8):87−93. [REN T T, YUE T L, WEI X, et al. Process optimization of apple pulp fermented by probiotics and analysis of volatile flavor components before and after fermentation[J]. Food Science,2019,40(8):87−93. doi: 10.7506/spkx1002-6630-20180423-290 REN T T, YUE T L, WEI X, et al. Process optimization of apple pulp fermented by probiotics and analysis of volatile flavor components before and after fermentation [J]. Food science, 2019, 40 (8): 87-93. doi: 10.7506/spkx1002-6630-20180423-290
[15]	KLOST M, DRUSCH S. Structure formation and rheological properties of pea protein-based gels[J]. Food Hydrocolloids,2019,94:622−630. doi: 10.1016/j.foodhyd.2019.03.030
[16]	XING G, GIOSAFATTO C, RUI X, et al. Microbial transglutaminase-mediated polymerization in the presence of lactic acid bacteria affects antigenicity of soy protein component present in bio-tofu[J]. Journal of Functional Foods,2019,53:292−298. doi: 10.1016/j.jff.2018.12.035
[17]	张珺, 喻婷, 许浩翔, 等. 乳酸菌发酵刺梨-猴头菇饮料的工艺优化[J]. 现代食品科技,2020,36(11):202−211. [ZHANG J, YU T, XU H X, et al. Process optimization of Rosa roxburghii erinaceus beverage fermented by lactic acid bacteria[J]. Modern Food Science and Technology,2020,36(11):202−211. ZHANG J, YU T, XU H X, et al. Process optimization of Rosa roxburghii erinaceus beverage fermented by lactic acid bacteria [J]. Modern food science and technology, 2020, 36 (11): 202-211.
[18]	MM A, AH A, ADG B, et al. Optimization of the viability of Lactobacillus acidophilus and physico-chemical, textural and sensorial characteristics of flaxseed-enriched stirred probiotic yogurt by using response surface methodology[J]. LWT-Food Science and Technology,2019,102:80−88. doi: 10.1016/j.lwt.2018.12.023
[19]	FERNANDES S, LIMA F S, RODRIGUES D, et al. Evaluation of the isoflavone and total phenolic contents of kefir-fermented soymilk storage and after the in vitro digestive system simulation[J]. Food Chemistry,2017:229−373.
[20]	王迪, 王颖, 张艳莉, 等. 芸豆酵素发酵过程中组分及抗氧化功能研究[J]. 食品工业科技,2021,42(18):18−24. [WANG D, WANG Y, ZHANG Y L, et al. Study on components and antioxidant function in kidney bean enzyme fermentation[J]. Science and Technology of Food Industry,2021,42(18):18−24. WANG D, WANG Y, ZHANG Y L, et al. Study on components and antioxidant function in kidney bean enzyme fermentation [J]. Food industry science and technology, 2021, 42 (18): 18-24.
[21]	DAI C, MA H, HE R, et al. Improvement of nutritional value and bioactivity of soybean meal by solid-state fermentation with Bacillus subtilis[J]. LWT-Food Science and Technology,2017,86:1−7. doi: 10.1016/j.lwt.2017.07.041
[22]	RODRIGUEZ-ROQUE M J, ROJAS-GRAUE M A, ELEZ-MARTINEZ P, et al. Soymilk phenolic compounds, isoflavones and antioxidant activity as affected by in vitro gastrointestinal digestion[J]. Food Chemistry,2013,136(1):206−212. doi: 10.1016/j.foodchem.2012.07.115
[23]	SUO H, QIAN Y, FENG X, et al. Free radical scavenging activity and cytoprotective effect of soybean milk fermented with L actobacillus fermentum Zhao[J]. Journal of Food Biochemistry,2016,40(3):294−303. doi: 10.1111/jfbc.12223
[24]	SANJUKTA S, RAI A K. Production of bioactive peptides during soybean fermentation and their potential health benefits[J]. Trends in Food Science & Technology,2016,50:1−10.
[25]	任海伟, 李志忠, 王鸣刚, 等. 超滤对芸豆蛋白酶解物抗氧化活性的影响[J]. 食品科学,2009(18):212−216. [REN H W, LI Z Z, WANG M G, et al. Effect of ultrafiltration on antioxidant activity of kidney bean protein hydrolysate[J]. Food Science,2009(18):212−216. doi: 10.3321/j.issn:1002-6630.2009.18.045 REN H W, LI Z Z, WANG M G, et al. Effect of ultrafiltration on antioxidant activity of kidney bean protein hydrolysate [J]. Food Science, 2009(18): 212-216. doi: 10.3321/j.issn:1002-6630.2009.18.045
[26]	BAN Q, LIU Z, YU C, et al. Physiochemical, rheological, microstructural, and antioxidant properties of yogurt using monk fruit extract as a sweetener[J]. Journal of Dairy Science,2020,103(11):10006−10014. doi: 10.3168/jds.2020-18703
[27]	KETNAWA S, OGAWA Y. Evaluation of protein digestibility of fermented soybeans and changes in biochemical characteristics of digested fractions[J]. Journal of Functional Foods,2019,52:640−647. doi: 10.1016/j.jff.2018.11.046
[28]	WANG M, LEI M, SAMINA N, et al. Impact of Lactobacillus plantarum 423 fermentation on the antioxidant activity and flavor properties of rice bran and wheat bran[J]. Food Chemistry,2020,330:127156. doi: 10.1016/j.foodchem.2020.127156
[29]	KENNAS A, AMELLAL-CHIBANE H, KESSAL F, et al. Effect of pomegranate peel and honey fortification on physicochemical, physical, microbiological and antioxidant properties of yoghurt powder[J]. Journal of the Saudi Society of Agricultural Sciences,2020,19(1):99−108. doi: 10.1016/j.jssas.2018.07.001
[30]	从彦丽, 彭梦雪, 王慧清, 等. 梨体外模拟胃肠消化过程中多酚、黄酮及抗氧化活性的变化规律[J]. 现代食品科技,2016,32(6):29−34. [CONG Y L, PENG M X, WANG H Q, et al. Changes of polyphenols, flavonoids and antioxidant activities in pear during gastrointestinal digestion in vitro[J]. Modern Food Science and Technology,2016,32(6):29−34. CONG Y L, PENG M X, WANG H Q, et al. Changes of polyphenols, flavonoids and antioxidant activities in pear during gastrointestinal digestion in vitro [J]. Modern Food Science and Technology, 2016, 32(6): 29-34.
[31]	陈晨, 贾才华, 赵思明, 等. 米发糕的体外模拟胃肠消化特性研究[J]. 中国粮油学报,2018,33(11):7−13. [CHEN C, JIA C H, ZHAO S M, et al. Study on simulated gastrointestinal digestion characteristics of rice cake in vitro[J]. Chinese Journal of grain and oil,2018,33(11):7−13. doi: 10.3969/j.issn.1003-0174.2018.11.003 CHEN C, JIA C H, ZHAO S M, et al. Study on simulated gastrointestinal digestion characteristics of rice cake in vitro [J]. Chinese Journal of grain and oil, 2018, 033 (11): 7-13. doi: 10.3969/j.issn.1003-0174.2018.11.003
[32]	ALENISAN M A, ALQATTAN H H, TOLBAH L S, et al. Antioxidant properties of dairy products fortified with natural additives: A review[J]. Journal of the Association of Arab Universities for Basic and Applied Sciences,2017,24:101−106.
[33]	陈树俊, 郑婕. 响应面优化开菲尔粒发酵复合果蔬汁工艺及体外消化特性分析[J]. 食品研究与开发,2020,41(3):150−158. [CHEN S J, ZHENG J. Response surface methodology optimization of kefir granule fermentation process and in vitro digestion characteristics of compound fruit and vegetable juice[J]. Food Research and Development,2020,41(3):150−158. CHEN S J, ZHENG J. Response surface methodology optimization of kefir granule fermentation process and in vitro digestion characteristics of compound fruit and vegetable juice [J]. Food research and development, 2020, 41 (3): 150-158.
[34]	HUSSEIN H, AWAD S, EL-SAYED I, et al. Impact of chickpea as prebiotic, antioxidant and thickener agent of stirred bio-yoghurt[J]. Annals of Agricultural Sciences,2020,65(1):49−58. doi: 10.1016/j.aoas.2020.03.001
[35]	STRATIL, KLEJDUS, KUBAN. Determination of phenolic compounds and their antioxidant activity in fruits and cereals[J]. Talanta,2007,71(4):1741−1751. doi: 10.1016/j.talanta.2006.08.012

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