Study on Extraction Process of Corn Steep Liquor Protein by Response Surface Methodology and Single Cell Protein Fermentation

LUO Yangqing; WU Zenglin; LI Shengfu; ZHANG Junjie; DUAN Rui

doi:10.13386/j.issn1002-0306.2021080230

Volume 43 Issue 10

May 2022

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Science and Technology of Food Industry > 2022 > 43(10): 231-237. > DOI: 10.13386/j.issn1002-0306.2021080230

LUO Yangqing, WU Zenglin, LI Shengfu, et al. Study on Extraction Process of Corn Steep Liquor Protein by Response Surface Methodology and Single Cell Protein Fermentation[J]. Science and Technology of Food Industry, 2022, 43(10): 231−237. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080230.

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Study on Extraction Process of Corn Steep Liquor Protein by Response Surface Methodology and Single Cell Protein Fermentation

LUO Yangqing^{1, 2,},
WU Zenglin³,
LI Shengfu^{1, 4},
ZHANG Junjie^{1, 4},
DUAN Rui^{1, 4, ,}

1.
College of Food Science & Technology, Jiangsu Ocean University, Lianyungang 222005, China
2.
Lianyungang Huanhai Chemical Co., Ltd., Lianyungang 222066, China
3.
Roquette China Co., Ltd., Lianyungang 222005, China
4.
Jiangsu Provincial Key Laboratory of Marine Biotechnology, Lianyungang 222005, China

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Received Date: August 22, 2021
Available Online: April 12, 2022

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Abstract

Abstract

The method of extracting protein from corn steep liquor by isoelectric point method was studied. The supernatant after extracting crude protein was used to produce single cell protein. Single factor experiment was used to analyze the influence of pH, temperature and time on protein extraction. Response surface methodology was used to determine the optimal process of isoelectric point extraction of protein. The conditions for the production of single cell protein from the supernatant of Saccharomyces cerevisiae and Candida utilis were studied. The results showed that the optimum conditions for crude protein extraction by isoelectric point method were as follows: pH 7.6, extraction temperature 26.4 ℃ and extraction time 20 min. The extraction rate was 77.5%. The conditions for producing single cell protein from the supernatant after extracting crude protein were as follows: Candida utilis was better than Saccharomyces cerevisiae. When the dry matter concentration in the supernatant was 8% and pH was 5.5, the colony number obtained by Candida utilis fermentation reached 1.6×10⁹ CFU/mL.
- corn steep liquor,
- protein,
- isoelectric point,
- response surface,
- Candida utilis

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References

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