Isolation and Identification of a Pathogen Causing Guava Black Spot

GUO Xiyuan; LAN Heying; LIN Yan; LIU Haoliang; CHEN Tuanwei

doi:10.13386/j.issn1002-0306.2021080223

Volume 43 Issue 10

May 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(10): 158-164. > DOI: 10.13386/j.issn1002-0306.2021080223

GUO Xiyuan, LAN Heying, LIN Yan, et al. Isolation and Identification of a Pathogen Causing Guava Black Spot[J]. Science and Technology of Food Industry, 2022, 43(10): 158−164. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080223.

Citation:

PDF (8485 KB)

Isolation and Identification of a Pathogen Causing Guava Black Spot

College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

More Information

Received Date: August 22, 2021
Available Online: March 12, 2022

Graphical Abstract

Abstract

Abstract

Objective: In order to provide scientific basis for further clarifying the species and characteristics of dominant pathogens causing guava black spot. Method: A dominant pathogen, named S1, was isolated and purified from the guava fruit infected with black spot disease, and subjected to morphology observation, pathogenicity determination, growth characteristics study and polymerase chain reaction (PCR) sequencing comparison analysis were conducted for comprehensive identification of the dominant pathogen S1. Results: According to the morphological observation, the aerial hyphae of the S1 colony were fluffy and dense, and the color was dark green or black. The spore morphology was monospore and transparent, and the size was uniform, about (6~10) μm×(6~7) μm, round or elliptical, compared with the "Fungus Identification Manual", it was preliminarily determined to belong to Phyllanthus spp., and once again inoculated to healthy guava fruits for pathogenicity determination. The results showed that S1 was the dominant pathogen causing guava black spot disease. Research on growth characteristics showed that S1 was more suitable for growth under alkaline, dark and normal temperature conditions. Moreover, S1 was identified as Phyllosticta capitalensis by the comparison of the PCR sequencing results. Conclusion: The dominant pathogen causing guava black spot disease was Phyllosticta capitalensis.
- Psidium guajava,
- pathogenic bacteria,
- identification,
- Phyllosticta capitalensis

FullText(HTML)

References (34)

References

[1]	刘建林, 夏明忠, 袁颖. 番石榴的综合利用现状及发展前景[J]. 中国林副特产,2005,4(6):60−62. [LIU Jianlin, XIA Mingzhong, YUAN Ying. Psidium guajava integrated utlizaiton and its development prospects in China[J]. Forest By-product and Speciality in China,2005,4(6):60−62. doi: 10.3969/j.issn.1001-6902.2005.06.036 LIU Jianlin, XIA Mingzhong, YUAN Ying. Psidium guajava integrated utlizaiton and its development prospects in China[J]. Forest By-product and Speciality in China, 2005, 4(6): 60-62. doi: 10.3969/j.issn.1001-6902.2005.06.036
[2]	彭燕, 张敏, 崔小丽, 等. 珍珠番石榴果实中的营养成分与活性物质分析[J]. 食品与机械,2020,36(8):36−40. [PENG Yan, ZHANG Min, CUI Xiaoli, et al. Analysis of nutrients and bioactive compounds in fruits of Psidium guajava l. cv. pearl[J]. Food & Machinery,2020,36(8):36−40. PENG Yan, ZHANG Min, CUI Xiaoli, et al. Analysis of nutrients and bioactive compounds in fruits of Psidium guajava l. cv. pearl[J]. Food & Machinery, 2020, 36(8): 36-40.
[3]	张朝坤, 黄婉莉, 陈洪彬, 等. 番石榴果实生长发育和营养品质变化规律分析[J]. 热带作物学报,2021,42(4):1035−1040. [ZHANG Chaokun, HUANG Wanli, CHEN Hongbin, et al. Analysis on the law of Psidium guajava L. fruit growth and nutritional quality[J]. Chinese Journal of Tropical Crops,2021,42(4):1035−1040. doi: 10.3969/j.issn.1000-2561.2021.04.018 ZHANG Chaokun, HUANG Wanli, CHEN Hongbin, et al. Analysis on the law of Psidium guajava L. fruit growth and nutritional quality[J]. Chinese Journal of Tropical Crops, 2021, 42(4): 1035-1040. doi: 10.3969/j.issn.1000-2561.2021.04.018
[4]	孙锐, 孙蕾, 赵登超, 等. 不同石榴品种果实的营养成分比较分析[J]. 食品工业科技,2015,36(2):358−361. [SUN Rui, SUN Lei, ZHAO Dengchao, et al. Comparative analysis of nutritional ingredients in different kinds of pomegranate fruits[J]. Science and Technology of Food Industry,2015,36(2):358−361. SUN Rui, SUN Lei, ZHAO Dengchao, et al. Comparative analysis of nutritional ingredients in different kinds of pomegranate fruits[J]. Science and Technology of Food Industry, 2015, 36(2): 358-361.
[5]	LI P Y, HSU C C, YIN M C, et al. Protective effects of red guava on inflammation and oxidative stress in streptozotocin-induced diabetic mice[J]. Molecules,2015,20(12):22341−22350. doi: 10.3390/molecules201219831
[6]	HIRUDKAR J R, PARMAR K M, PRASAD R S, et al. The antidiarrhoeal evaluation of Psidium guajava L. against enteropathogenic Escherichia coli induced infectious diarrhoea[J]. Journal of Ethnopharmacology,2020,251:112561. doi: 10.1016/j.jep.2020.112561
[7]	宋银雪, 高烨, 许静, 等. 番石榴叶总三萜对脓毒症大鼠急性肾损伤的治疗及对PI3K/AKT通路的调控[J]. 河北医药,2021,43(15):2270−2274. [SONG Yinxue, GAO Ye, XU Jing, et al. Therapeutic effects of total triterpenoids in guava leaves on acute kidney injury in septic rats and its regulation of PI3K/AKT pathway[J]. Hebei Medical Journal,2021,43(15):2270−2274. doi: 10.3969/j.issn.1002-7386.2021.15.006 SONG Yinxue, GAO Ye, XU Jing, et al. Therapeutic effects of total triterpenoids in guava leaves on acute kidney injury in septic rats and its regulation of PI3K/AKT pathway[J]. Hebei Medical Journal, 2021, 43(15): 2270-2274. doi: 10.3969/j.issn.1002-7386.2021.15.006
[8]	REZAEI M, LIU B. Food loss and waste in the food supply chain[J]. International Nut and Dried Fruit Council: Reus, Spain,2017:26−27.
[9]	HWANG S G, LI Y Y, LIN H L. Excellent nutritional value in fruits of three guava cultivars in Taiwan[J]. Acta Horticulturae,2017,1166:209−213.
[10]	周浓, 杨锡洪, 解万翠, 等. “珍珠”番石榴的营养成分与挥发性风味特征分析[J]. 食品与机械,2016,32(2):37−40. [ZHOU Nong, YANG Xihong, XIE Wangcui, et al. Analysis of nutrition and valitile flavor of guava fruit (Psidium guajava L.)[J]. Food & Machinery,2016,32(2):37−40. ZHOU Nong, YANG Xihong, XIE Wangcui, et al. Analysis of nutrition and valitile flavor of guava fruit (Psidium guajava L. )[J]. Food & Machinery, 2016, 32(2): 37-40.
[11]	PRUSKY D. Reduction of the incidence of postharvest quality losses, and future prospects[J]. Food Security,2011,3(4):463−474. doi: 10.1007/s12571-011-0147-y
[12]	张慧丽, 徐瑛, 段维军, 等. 番石榴黑斑病病原菌的分离鉴定[J]. 安徽农业科学,2011,39(28):17310−17311. [ZHANG Huili, XU Ying, DUAN Weijun, et al. Isolation and identification of black spot pathogen in Psidium guajava[J]. Journal of Anhui Agricultural Sciences,2011,39(28):17310−17311. doi: 10.3969/j.issn.0517-6611.2011.28.073 ZHANG Huili, XU Ying, DUAN Weijun, et al. Isolation and identification of black spot pathogen in Psidium guajava[J]. Journal of Anhui Agricultural Sciences, 2011, 39(28): 17310-17311. doi: 10.3969/j.issn.0517-6611.2011.28.073
[13]	赵志常, 黄建峰. 番石榴炭疫病[J]. 世界热带农业信息,2019(8):43. [ZHAO Zhichang, HUANG Jianfeng. Guava anthracnose[J]. World Tropical Agriculture Information,2019(8):43. ZHAO Zhichang, HUANG Jianfeng. Guava Anthracnose[J]. World Tropical Agriculture Information, 2019, (8): 43.
[14]	SOLARTE F, MUÑOZ C G, MAHARACHCHIKUMBURA S S N, et al. Diversity of Neopestalotiopsis and Pestalotiopsis spp., causal agents of guava scab in colombia[J]. Plant Disease,2018,102(1):49−59. doi: 10.1094/PDIS-01-17-0068-RE
[15]	高新明, 李本金, 兰成忠, 等. 番石榴焦腐病菌的ITS分析及PCR检测[J]. 植物保护学报,2011,38(3):227−232. [GAO Xinming, LI Benjin, LAN Chengzhong, et al. Analysis of ITS sequence and PCR detection of Botryosphaeria rhodina[J]. Journal of Plant Protection,2011,38(3):227−232. GAO Xinming, LI Benjin, LAN Chengzhong, et al. Analysis of ITS sequence and PCR detection of Botryosphaeria rhodina[J]. Journal of Plant Protection, 2011, 38(3): 227-232.
[16]	董汉松. 植病研究法[M]. 北京: 中国农业出版社, 2012 DONG Hansong. Plant disease research method[M]. Beijing: China Agriculture Press, 2012.
[17]	SANGER F, COULSON A R, BARRELL B G, et al. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing[J]. Journal of Molecular Biology,1980,143(2):161−178. doi: 10.1016/0022-2836(80)90196-5
[18]	ARAFAT K. A novel isolate of Phyllosticta capitalensis causes black spot disease on guava fruit in Egypt[J]. Asian Journal of Plant Pathology,2018,12(1):27−37.
[19]	SRISKULTEIW N. 番石榴黑星病之鉴定及感染源[D]. 台中: 中兴大学, 2014. SRISKULTEIW N. Identification and infection source of guava scab[D]. Taizhong: Zhongxing University, 2014.
[20]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979 WEI Jingchao. Fungus identification manual[M]. Shanghai: Shanghai Science and Technology Press, 1979.
[21]	孙俊. 榛子叶斑病病原菌生物学特性[J]. 江苏农业科学,2017,45(7):101−103. [SUN Jun. Biological characteristics of the pathogen of hazelnut leaf spot[J]. Jiangsu Agricultural Sciences,2017,45(7):101−103. SUN Jun. Biological characteristics of the pathogen of hazelnut leaf spot[J]. Jiangsu Agricultural Sciences, 2017, 45(7): 101-103.
[22]	王晓梅, 黄琦, 李玉. 桂花叶斑病病原鉴定及其生物学特性研究[J]. 吉林农业大学学报,2011,33(2):151−157. [WANG Xiaomei, HUANG Qi, LI Yu. Pathogeny identification and biological characteristics of the leaf spot lesions of Osmamthus fragrans[J]. Journal of Jilin Agricultural University,2011,33(2):151−157. WANG Xiaomei, HUANG Qi, LI Yu. Pathogeny identification and biological characteristics of the leaf spot lesions of Osmamthus fragrans[J]. Journal of Jilin Agricultural University, 2011, 33(2): 151-157.
[23]	张桥. 桂花叶枯病病原菌生物学特性研究[J]. 现代农业科技,2016(11):153−154. [ZHANG Qiao. Study on the biological characteristics of the pathogen of Osmanthus fragrans leaf blight[J]. Modern Agricultural Science and Technology,2016(11):153−154. doi: 10.3969/j.issn.1007-5739.2016.11.097 ZHANG Qiao. Study on the biological characteristics of the pathogen of Osmanthus fragrans leaf blight[J]. Modern Agricultural Science and Technology, 2016(11): 153-154. doi: 10.3969/j.issn.1007-5739.2016.11.097
[24]	王正贤, 朱文倩, 黄奕蔓, 等. 广西玉林市番石榴焦腐病的病原菌鉴定[J]. 植物病理学报,2021,51(5):813−816. [WANG Zhengxian, ZHU Wenqian, HUANG Yiman, et al. Identification of the pathogen causing guava black-rot in Yulin, Guangxi[J]. Acta Phytopathologica Sinica,2021,51(5):813−816. WANG Zhengxian, ZHU Wenqian, HUANG Yiman, et al. Identification of the pathogen causing guava black-rot in Yulin, Guangxi[J]. Acta Phytopathologica Sinica, 2021, 51(5): 813-816.
[25]	刘任, 戚佩坤. 广州地区番石榴病害鉴定[J]. 仲恺农业技术学院学报,1991,4(1):38−46. [LIU Ren, QI Peikun. Identification on the diseases of guava in Guangzhou District[J]. Journal of Zhongkai Agrotechnical College,1991,4(1):38−46. LIU Ren, QI Peikun. Identification on the diseases of guava in Guangzhou District[J]. Journal of Zhongkai Agrotechnical College, 1991, 4(1): 38-46.
[26]	LAN C, YAO J, YANG X, et al. Specific and sensitive detection of the guava fruit anthracnose pathogen (Colletotrichum gloeosporioides) by loop-mediated isothermal amplification (LAMP) assay[J]. Microbiology,2020,66(1):17−24.
[27]	张志华, 洪葵. 核酸序列直接分析在真菌鉴定方面的应用[J]. 华南热带农业大学学报,2006,12(2):39−43. [ZHANG Zhihua, HONG Kui. Application of nucleic ACID sequence analysis in fungi taxonmy[J]. Journal of South China University of Tropical Agrjculture,2006,12(2):39−43. ZHANG Zhihua, HONG Kui. Application of nucleic ACID sequence analysis in fungi taxonmy[J]. Journal of South China University of Tropical Agrjculture, 2006, 12(2): 39-43.
[28]	SAOWANEE W, LORENZO L, PEDRO W, et al. Phylosticta capialensis, a widespread endophyte of plants[J]. Fungal Diversity,2013,60(1):91−105. doi: 10.1007/s13225-013-0235-8
[29]	赵杏利, 胡镇杰, 侯典云, 等. 牡丹内生真菌MD76的鉴定及次级代谢产物研究[J]. 中国药学杂志,2018,53(21):1826−1830. [ZHAO Xingli, HU Zhenjie, HOU Dianyun, et al. Identification and secondary metabolites of endophytic fungus MD76 from Paeonia suffruticosa[J]. Chinese Pharmaceutical Journal,2018,53(21):1826−1830. ZHAO Xingli, HU Zhenjie, HOU Dianyun, et al. Identification and secondary metabolites of endophytic fungus MD76 from Paeonia suffruticosa[J]. Chinese Pharmaceutical Journal, 2018, 53(21): 1826-1830.
[30]	张建芬, 林璟, 徐好仪, 等. 华泽兰内生真菌的分离及其抑菌、抗肿瘤活性[J]. 中成药,2020,42(1):133−138. [ZHANG Jianfen, LIN Jing, XU Haoyi, et al. Isolation of endophytic fungi from eupatorium Chinense and their antimicrobial, antitumor activities[J]. Chinese Traditional Patent Medicine,2020,42(1):133−138. doi: 10.3969/j.issn.1001-1528.2020.01.029 ZHANG Jianfen, LIN Jing, XU Haoyi, et al. Isolation of endophytic fungi from eupatorium Chinense and their antimicrobial, antitumor activities[J]. Chinese Traditional Patent Medicine, 2020, 42(1): 133-138. doi: 10.3969/j.issn.1001-1528.2020.01.029
[31]	DUAN C H, CHANG C M, SU C C, et al. Phyllosticta capitalensis causes black spot on persimmon (Diospyros kaki) fruit in Taiwan[J]. Australasian Plant Disease Notes,2017,12(1):1−4. doi: 10.1007/s13314-016-0226-1
[32]	PURBAJANTI E D, SETIADI A, ROESSALI W. Variability and nutritive compounds of guava (Psidium guajava L.)[J]. Indian Journal of Agricultural Research,2016,50(3):273−277.
[33]	SOARES-COLLETTI A R, FISCHER I H, LOURENÇO S D A. The effects of temperature and wetness duration on the development of Guignardia psidii in guava fruit naturally infected[J]. Australasian Plant Pathology,2015,44(4):413−418. doi: 10.1007/s13313-014-0327-2
[34]	肖倩莼, 陈永强, 余卓桐, 等. 主要热带果树煤烟病的为害性及病原菌种类研究[J]. 热带作物学报,2000,21(1):25−30. [XIAO Qianchun, CHEN Yongqiang, YU Zhuodong, et al. A study on sooty moulds of tropical fruit crops and their pathogens[J]. Chinese Journal of Tropical Crops,2000,21(1):25−30. doi: 10.3969/j.issn.1000-2561.2000.01.005 XIAO Qianchun, CHEN Yongqiang, YU Zhuodong, et al. A Study on sooty moulds of tropical fruit crops and their pathogens[J]. Chinese Journal of Tropical Crops, 2000, 21(1): 25-30. doi: 10.3969/j.issn.1000-2561.2000.01.005

Supplements (0)

Cited By

Cited by

Periodical cited type(24)

1.	骆春萍，蔡树芸，黄雅瑜，林米妮，郑海龙，杨光乐，张怡评. 岩藻多糖制备工艺及其在护肤品中的应用进展. 香料香精化妆品. 2025(02): 23-28+110 .
2.	刘松，刘红全，李慧敏，徐程浩，王燕姿，藏颖. 拟微绿球藻胞内多糖提取条件优化及其生物活性研究. 食品科技. 2024(03): 233-242 .
3.	刘欣雨，胡蔚然，李铮凯，吉玮，倪潇. 藻胶实验室：澎湃荧光海. 大学化学. 2024(05): 396-404 .
4.	沈康，郭瑞成，徐天旭，王伟华. DEAE-52纤维素柱层析纯化处理对西梅可溶性膳食纤维的影响. 食品与发酵工业. 2024(17): 209-217 .
5.	张田，黄雨洋，刘琳琳，吕铭守，朱颖，孙冰玉，朱秀清. 多糖对高水分挤压植物蛋白肉结构及品质影响的研究进展. 食品科学. 2024(22): 341-350 .
6.	Changhui YAN，Mingxuan PAN，Lihua GENG，Quanbin ZHANG，Yadong HU，Jing WANG，Sujuan YE. A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities. Journal of Oceanology and Limnology. 2024(06): 1998-2012 .
7.	唐天城，朱本伟，姚忠，孙芸，熊强. 石莼多糖及其寡糖结构、制备及活性的研究进展. 现代食品科技. 2023(05): 340-353 .
8.	唐翠娥，周培源，刘延照，李艳. 基于原子力显微镜研究黄原胶分子结构的云母片处理方法优化. 食品工业科技. 2023(14): 60-66 . 本站查看
9.	邱智超，勾宇春，张志鹏，王宗抗，孟品品，周进，姜玥璐. 藻类资源在农业种植业中的应用研究进展. 农业资源与环境学报. 2023(04): 840-851 .
10.	张琨霖，王贺琪，郭庆彬，刘欢欢，梁宏合，王乐，李贞景. 桑黄子实体多糖的提取工艺优化、结构解析及免疫活性分析. 食品工业科技. 2023(20): 93-100 . 本站查看
11.	黄卫红，董乐. 紫球藻胞外多糖酶解物的纯化、红外鉴定及其抗氧化活性. 泉州师范学院学报. 2023(05): 19-27 .
12.	曾凡珂，潘蕾蔓，张祎，赖富饶，吴晖，张猛猛. 荸荠皮多糖的理化性质及抗氧化活性. 现代食品科技. 2022(03): 82-88+81 .
13.	王朝群，杨燕，唐超，何希瑞. 天麻多糖提取分离方法和药理作用研究进展. 中国药事. 2022(04): 417-428 .
14.	杨斯惠，向月，曹亚楠，任远航，彭镰心，时小东. 植物多糖的益生作用及其影响因素研究进展. 食品科学. 2022(11): 301-310 .
15.	刘亚楠，李欢，蒋凡，傅玲琳，王彦波. 基于活性包装视角下的水产品保鲜机制研究进展. 食品科学. 2022(13): 285-291 .
16.	陈超，谭书明，王画，杨笙，代晓桐. 刺梨及其活性成分对2型糖尿病小鼠糖脂代谢的影响. 食品科学. 2022(13): 146-154 .
17.	郭建行，贾颂华，李博润，李珊珊，冀晓龙，刘延奇. 红海藻多糖提取、分离纯化及生物活性研究进展. 食品研究与开发. 2022(16): 216-224 .
18.	孙艳宾，李宁，梁君玲，张慧婧，景大为，张川. 海藻酸钠提取工艺研究进展. 食品科技. 2022(08): 201-206 .
19.	贾薇，余冬生，余养朝，冯占，胡明，张劲松，汪雯翰. 三相分离法制备金针菇提取物中多糖的理化性质及体外活性. 食用菌学报. 2022(05): 73-80 .
20.	孔丽，朱月霞，周冰雪，刘志，薛旺迁，石锦峰，郑家勤，李姣姣，吉敬，秦昆明，董自波，沈金阳. 海蒿子多糖提取、纯化及其药理活性研究进展. 大连海洋大学学报. 2022(05): 894-902 .
21.	高玥，许倩楠，蔡明刚，胡贤陈，田文静，陈海峰. 海洋来源药食同源品开发利用研究进展. 中草药. 2021(17): 5455-5464 .
22.	陆伊俏，池海波，厉桂宁，陈纤，邱家港，余辉，方旭波，陈小娥. 可口革囊星虫体腔液多糖碱提工艺优化及其抗氧化性研究. 食品工业科技. 2021(19): 204-210 . 本站查看
23.	刘小杰，倪辉. 海藻多糖在乳品工业中的应用研究进展. 乳业科学与技术. 2021(06): 31-38 .
24.	林晓娟，苏志琛，陈继承. 海带多糖的结构特征、生物活性及其应用. 现代食品. 2021(24): 49-52 .