Effects of Acidic/Alkali Pretreatment on Physicochemical Properties and Gelation Characteristics of Bone Gelatin from American Eel (<i>Anguilla rostrata</i>)

SUN Lechang; ZHAO Ayun; DU Han; WENG Ling; MIAO Song; CAO Minjie

doi:10.13386/j.issn1002-0306.2021080130

Volume 43 Issue 9

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(9): 71-78. > DOI: 10.13386/j.issn1002-0306.2021080130

SUN Lechang, ZHAO Ayun, DU Han, et al. Effects of Acidic/Alkali Pretreatment on Physicochemical Properties and Gelation Characteristics of Bone Gelatin from American Eel (Anguilla rostrata)[J]. Science and Technology of Food Industry, 2022, 43(9): 71−78. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080130.

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Effects of Acidic/Alkali Pretreatment on Physicochemical Properties and Gelation Characteristics of Bone Gelatin from American Eel (Anguilla rostrata)

SUN Lechang^{1, 2, 3,},
ZHAO Ayun^{1, 2, 3},
DU Han^{1, 4},
WENG Ling^{1, 2, 3},
MIAO Song^{1, 4},
CAO Minjie^{1, 2, 3, ,}

1.
College of Marine Food and Bioengineering, Jimei University, Xiamen 361021, China
2.
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China
3.
National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Jimei University, Xiamen 361021
4.
Teagasc Food Research Centre, Ministry of Agriculture, Cork 999014, Ireland

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Received Date: August 11, 2021
Available Online: March 06, 2022

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Abstract

Fish bone gelatins were prepared from Anguilla rostrata by acidic or alkali pretreatment combined with hot water extraction. The physicochemical properties and gel properties of fish bone gelatins were investigated via the yield determination, gel strength measurement, SDS-PAGE analysis, ultraviolet full-wavelength scanning, infrared spectrum scanning, dynamic rheology measurement and scanning electron microscopy. As results, the yields of acid pretreated gelatin (AG60) and alkaline pretreated gelatin (BG60) were 13.6% and 6.88%, with the corresponding gel strengths of 101.95 g and 78.74 g, respectively. The hydroxyproline contents of AG60 and BG60 were 3.2 g/100 g and 2.7 g/100 g. Both gelatins were mainly consisted of β and α₁, α₂ chains, the contents of α₁ and α₂ chains in AG60 was significantly higher than those in BG60. Both AG60 and BG60 possessed the characteristic absorption peak of gelatin without any absorption peaks for other proteins. The AG60 possessed higher gelling temperature and melting temperature, as well as a shorter gelling time. Scanning electron microscopy analysis showed that AG60 showed a more dense and uniform gel network structure as compared with BG60. Present study suggested that the eel bone gelatin prepared by acidic pretreatment had better gel properties than that of gelatin prepared by alkali pretreatment.
- American eel,
- fish bone gelatin,
- acidic pretreatment,
- alkali pretreatment,
- gelation characteristics

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[1]	KARIM A A, BHAT R. Fish gelatin: Properties, challenges, and prospects as an alternative to mammalian gelatins[J]. Food Hydrocolloids,2009,23(3):563−576. doi: 10.1016/j.foodhyd.2008.07.002
[2]	KIM S K, MENDIS E. Bioactive compounds from marine processing byproducts-A review[J]. Food Research International,2006,39(4):383−393. doi: 10.1016/j.foodres.2005.10.010
[3]	BORAN G, REGENSTEIN J M. Optimization of gelatin extraction from silver carp skin[J]. Journal of Food Science,2009,74(8):E432−E441. doi: 10.1111/j.1750-3841.2009.01328.x
[4]	DJABOUROV M, LECHAIRE J P, GAILL F. Structure and pheology of gelatin and collagen gels[J]. Biorheology,1993,30(3-4):191−205. doi: 10.3233/BIR-1993-303-405
[5]	HAO S, LI L, YANG X, et al. The characteristics of gelatin extracted from sturgeon (Acipenser baeri) skin using various pretreatments[J]. Food Chemistry,2009,115(1):124−128. doi: 10.1016/j.foodchem.2008.11.080
[6]	ALI A M M, KISHIMURA H, BENJAKUL S. Physicochemical and molecular properties of gelatin from skin of golden carp (Probarbus jullieni) as influenced by acid pretreatment and prior-ultrasonication[J]. Food Hydrocolloids,2018,82:164−172. doi: 10.1016/j.foodhyd.2018.03.052
[7]	于淑池, 赖卓慧. 金鲳鱼骨明胶的提取工艺及性质研究[J]. 食品研究与开发,2020,41(3):159−165. [YU Shuchi, LAI Zhuohui. Study on extraction technology and properties of bone gelatin from pomfret[J]. Food Research and Development,2020,41(3):159−165.
[8]	MUYONGA J H, COLE C, DUODU K G. Extraction and physico-chemical characterisation of nile perch (Lates niloticus) skin and bone gelatin[J]. Food Hydrocolloids,2004,18(4):581−592. doi: 10.1016/j.foodhyd.2003.08.009
[9]	MI H, WANG C, CHEN J, et al. Characteristic and functional properties of gelatin from the bones of alaska pollock (Theragra chalcogramma) and yellowfin sole (Limanda aspera) with papain-aided process[J]. Journal of Aquatic Food Product Technology,2019,28(3):287−297. doi: 10.1080/10498850.2019.1577933
[10]	葛蕾丹, 王琰婷, 乔雯. 中国烤鳗出口现状与竞争力分析[J]. 对外经贸,2020(1):28−30. [GE Leidan, WANG Yanting, QIAO Wen. Export status and competitiveness analysis of roast eel in China[J]. Foreign Economic Relations Trade,2020(1):28−30. doi: 10.3969/j.issn.2095-3283.2020.01.007
[11]	BANDEIRA S F, SILVA R S G, MOURA J M, et al. Characterization and film-forming properties of gelatins from whitemouth croaker (Micropogonias furnieri) skin and bones[J]. Journal of Aquatic Food Product Technology,2017,26(4):447−456. doi: 10.1080/10498850.2016.1210268
[12]	ABEDINIA A, ARIFFIN F, HUDA N, et al. Extraction and characterization of gelatin from the feet of Pekin duck (Anas platyrhynchos domestica) as affected by acid, alkaline, and enzyme pretreatment[J]. International Journal of Biological Macromolecules,2017,98:586−594. doi: 10.1016/j.ijbiomac.2017.01.139
[13]	LADISLAUS K M. 鱼皮、鱼骨中明胶的提取研究[D]. 无锡: 江南大学, 2006 LADISLAUS K M. Study on the extraction of gelatin from fish skin and fish bone[D]. Wuxi: Jangnan University, 2006.
[14]	WOESSNER J F J. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid[J]. Archives of Biochemistry and Biophysics,1961,93:440−447. doi: 10.1016/0003-9861(61)90291-0
[15]	TAN C, KARIM A A, UTHUMPORN U, et al. Effect of extraction temperature on the physicochemical properties of gelatin from the skin of black tilapia (Oreochromis mossambicus)[J]. Journal of Physical Science,2019,30(Supp.1):1−21. doi: 10.21315/jps2019.30.s1.1
[16]	LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227(5259):680−685. doi: 10.1038/227680a0
[17]	王珊珊, 卢玉坤, 杨霞, 等. 提取温度与提取时间对真鳕鱼骨明胶理化性质的影响[J]. 食品工业科技,2013,34(13):53−57. [WANG Shanshan, LU Yukun, YANG Xia, et al. Effect of extraction temperature and time on physicochemical properties of cod bone gelatin[J]. Science and Technology of Food Industry,2013,34(13):53−57.
[18]	KITTIPHATTANABAWON P, BENJAKUL S, VISESSANGUAN W, et al. Comparative study on characteristics of gelatin from the skins of brownbanded bamboo shark and blacktip shark as affected by extraction conditions[J]. Food Hydrocolloids,2010,24(2-3):164−171. doi: 10.1016/j.foodhyd.2009.09.001
[19]	郑惠彬. 鱼鳞明胶的提取及其成膜性能的研究[D]. 厦门: 集美大学, 2014 ZHENG Huibin. Extraction and film-forming properties of fish scale gelatin [D]. Xiamen: Jimei University, 2014.
[20]	SOCKALINGAM K, ABDULLAH H Z. Extraction of high value added gelatin biopolymer from black tilapia (Oreochromis mossambicus) head bones [C]// AIP Conference Proceedings. 2015.
[21]	杜杰, 刘廷薇, 马良, 等. 微波-快速冻融耦合鱼皮明胶理化性质分析[J]. 食品科学,2021,42(11):108−115. [DU Jie, LIU Tingwei, MA Liang, et al. Analysis of physicochemical properties of microwave-fast freeze-thaw coupled fish skin gelatin[J]. Food Science,2021,42(11):108−115. doi: 10.7506/spkx1002-6630-20200612-161
[22]	ZHANG T, SUN R, DING M, et al. Effect of extraction methods on the structural characteristics, functional properties, and emulsion stabilization ability of tilapia skin gelatins[J]. Food Chemistry,2020,328(30):127114.
[23]	AYKIN-DINCER E, KOC A, ERBAS M. Extraction and physicochemical characterization of broiler (Gallus gallus domesticus) skin gelatin compared to commercial bovine gelatin[J]. Poultry Science,2017,96(11):4124−4131. doi: 10.3382/ps/pex237
[24]	AKITA M, NISHIKAWA Y, SHIGENOBU Y, et al. Correlation of proline, hydroxyproline and serine content, denaturation temperature and circular dichroism analysis of type I collagen with the physiological temperature of marine teleosts[J]. Food Chemistry,2020,329(1):126775.
[25]	詹丽, 陈雪岚. 鱼明胶特性及改性对其品质影响的研究进展[J]. 食品工业科技, 2021, 42(23): 385-393 ZHAN Li, CHEN Xuelan. Research progress on characteristics of fish gelatin and effects of modification on its quality[J]. Science and Technology of Food Industry, 2021, 42(23): 385-393.
[26]	TAN C, KARIM A A, UTHUMPORN U, et al. Effect extraction temperature on the emulsifying properties of gelatin from black tilapia (Oreochromis mossambicus) skin[J]. Food Hydrocolloids,2020,108(3):106024.
[27]	KITTIPHATTANABAWON P, BENJAKUL S, SINTHUSAMRAN S, et al. Gelatin from clown featherback skin: Extraction conditions[J]. LWT-Food Science and Technology,2016,66:186−192. doi: 10.1016/j.lwt.2015.10.029
[28]	蔡成魁, 滕军. 明胶浸灰促进剂对浸灰周期的影响[J]. 明胶科学与技术,1999(4):205−208. [CAI Chengkui, TENG Jun. Effect of gelatin leaching promoter on leaching cycle[J]. The Science and Technology of Gelatin,1999(4):205−208.
[29]	YAN M, LI B, ZHAO X, et al. Characterization of acid-soluble collagen from the skin of walleye pollock (Theragra chalcogramma)[J]. Food Chemistry,2008,107(4):1581−1586. doi: 10.1016/j.foodchem.2007.10.027
[30]	钟朝辉, 李春美, 顾海峰, 等. 温度对鱼鳞胶原蛋白二级结构的影响[J]. 光谱学与光谱分析,2007(10):1970−1976. [ZHONG Chaohui, LI Chunmei, GU Haifeng, et al. Effect of temperature on secondary structure of fish scale collagen[J]. Spectroscopy and Spectral Analysis,2007(10):1970−1976.
[31]	PLEPIS A, GOISSIS G, Das GUPTA D K. Dielectric and pyroelectric characterization of anionic and native collagen[J]. Polymer Engineering and Science,1996,36(24):2932−2938. doi: 10.1002/pen.10694
[32]	DOYLE B B, BENDIT E G, BLOUT E R. Infrared spectroscopy of collagen and collagen-like polypeptides[J]. Biopolymers,1975,14(5):937−957. doi: 10.1002/bip.1975.360140505
[33]	LIU Y, XIA L, JIA H, et al. Physiochemical and functional properties of chum salmon (Oncorhynchus keta) skin gelatin extracted at different temperatures[J]. Journal of the Science of Food and Agriculture,2017,97(15):5406−5413. doi: 10.1002/jsfa.8431
[34]	CHIOU B, AVENA-BUSTILLOS R J, SHEY J, et al. Rheological and mechanical properties of cross-linked fish gelatins[J]. Polymer,2006,47(18):6379−6386. doi: 10.1016/j.polymer.2006.07.004
[35]	PARK J, CHOE J, KIM H, et al. Effects of various extraction methods on quality characteristics of duck feet gelatin[J]. Korean Journal for Food Science of Animal Resources,2013,33(2):162−169. doi: 10.5851/kosfa.2013.33.2.162
[36]	BENJAKUL S, OUNGBHO K, VISESSANGUAN W, et al. Characteristics of gelatin from the skins of bigeye snapper, Priacanthus tayenus and Priacanthus macracanthus[J]. Food Chemistry,2009,116(2):445−451. doi: 10.1016/j.foodchem.2009.02.063
[37]	满泽洲, 涂宗财, 王辉, 等. 加工条件对鱼鳞明胶凝胶性能和微观结构的影响[J]. 食品科学,2013,34(23):67−71. [MAN Zezhou, TU Zongcai, WANG Hui, et al. Effect of processing conditions on gel properties and microstructure of fish scale gelatin[J]. Food Science,2013,34(23):67−71.

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Effects of Acidic/Alkali Pretreatment on Physicochemical Properties and Gelation Characteristics of Bone Gelatin from American Eel (Anguilla rostrata)

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